Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 57236906

GI number: 57236906

Start: 1767403

End: 1768938

Strand: Reverse

Name: leuA [H]

Synonym: CJE1889

Alternate gene names: 57236906

Gene position: 1768938-1767403 (Counterclockwise)

Preceding gene: 57236908

Following gene: 57236905

Centisome position: 99.5

GC content: 34.7

Gene sequence:

>1536_bases
ATGAAAGATAATAAAATCATAATCTTTGATACCACTTTACGAGATGGAGAACAAGCTTTAGGTAGTTCTTTAGGTATCAA
TCAAAAATTACAAATTGCATTAGCGCTTGAAAATTTAGGCGTAGACGTTATAGAAGCAGGTTTTCCGGTATCTTCACAAG
GGGATTTTAAAGCGGTGCAAAAGATCGCTTCTAAGGTGAAAAACTCAACTATTTGTGCACTTTCTAGAGCATTGGATAAA
GATATAGATATGGCTTATGAAGCTTTAAAAGTGGCAAAGCATTTTAGAATTCATACTTTTATAGCCACTTCGACTTTGCA
TATGCAAGATAAATTAAAAAAAGATTTTGATGAAATTTTGTCTATGGCAAAAAGAGCGATTATAAGAGCAAGATCTTATA
CTGATGATGTAGAATTCTCATGCGAAGATGCAGGAAGAACTCCGATAGATAATCTTTGTTTTATGGTGGAAAATGCGATA
AAAGCAGGCGCTAAAACTATAAATATCCCAGATACCGTAGGCTATACCTTGCCAAGTGAATTTGCTAATATCATCAAAAT
TTTATTTAACAAAGTGCCAAATATAGACAAAGCTATCATTTCTGTGCATTGTCACAATGACTTAGGTATGGCAACGGGCA
ATAGCTTAAGTGCTATTTTACAAGGTGCAAGACAGATAGAGTGCACTATAAATGGACTTGGCGAAAGGGCGGGCAATTGT
GCTTTAGAAGAAGTGGTTATGGCGATAAAAACTAGGAAAGATTATTTAAAAGGTTTTTATACTGATATTAAATGTGAAAA
TATCTTTAAAACTTCAAAATTAGTTTCAGCTATCACTAATGAAAGCATACCTTCGCATAAAGCTATAGTGGGTAGCAATG
CGTTTTCTCATAGTTCAGGCATTCATCAAGATGGAGTGCTTAAAAATAGGCAAACTTACGAGATCATAAGCCCTAGTGCC
ATAGGAATTCATGACAATCGTATGTTAATGACGGCAAGAAGTGGAAGGGCTATGATAAAAACTTGCCTTGAAAATTTAGG
CTATGATGAAAATACTTATAATTTAGATGATGTTTATGAGCGTTTTTTACGCCTTGCGGATAAAAAGGGTCAAGTATATG
ATTATGATTTGGAAGCTTTGATGTTTTTAAGCTATGAAAATGAAGAAGAAAATGAATTTGTGATTGAAAAATTAAGCGTG
ATTAGTGGCAATATCCCAACCGCTTGTGTTTGTATGCGTATCAAAGAAGAGCTAAAAACTGAAGCTTGCACAGGAAATGG
TCCTGTTGAAGCAGTTTTTAACTGCATTGCTAGGATAACAAATTTAAAGCCTGTGTTAAAAGCTTATAGTATCAATGCAA
AAAGTTCAGGAGTAGATGCTCAAGGGCAAGTGGATGTGGATTTGGAATTTAAAGGGAGAAAATTCCATGGAAAAGGACTT
TCAACAGATGTTATAGAAGCTTCAGCACAAGCTTTTGCGAGTGCTTACAATGCGATTTATCGCTCTTTAAAAGTAGAAGA
AAGGAAAATGGCATGA

Upstream 100 bases:

>100_bases
TTATTTTTAATCATTTTTTACATTTTTAATTTTTTATCATTGTTGTTAAGTTTTTGAATTTTTAATTCATTCAGTCTGTT
TGGTTTTAAAGGATTAAATC

Downstream 100 bases:

>100_bases
AAGCTTATAAAGTAGCTGTTTTAGCAGGAGATGGCATAGGGCCTTTGGTGATGAAAGAAGCTTTAAAAATTTTAACTTTT
ATCTCACAAAAATACAATTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKDNKIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQKIASKVKNSTICALSRALDK
DIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEILSMAKRAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAI
KAGAKTINIPDTVGYTLPSEFANIIKILFNKVPNIDKAIISVHCHNDLGMATGNSLSAILQGARQIECTINGLGERAGNC
ALEEVVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSGIHQDGVLKNRQTYEIISPSA
IGIHDNRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYERFLRLADKKGQVYDYDLEALMFLSYENEEENEFVIEKLSV
ISGNIPTACVCMRIKEELKTEACTGNGPVEAVFNCIARITNLKPVLKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGL
STDVIEASAQAFASAYNAIYRSLKVEERKMA

Sequences:

>Translated_511_residues
MKDNKIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQKIASKVKNSTICALSRALDK
DIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEILSMAKRAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAI
KAGAKTINIPDTVGYTLPSEFANIIKILFNKVPNIDKAIISVHCHNDLGMATGNSLSAILQGARQIECTINGLGERAGNC
ALEEVVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSGIHQDGVLKNRQTYEIISPSA
IGIHDNRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYERFLRLADKKGQVYDYDLEALMFLSYENEEENEFVIEKLSV
ISGNIPTACVCMRIKEELKTEACTGNGPVEAVFNCIARITNLKPVLKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGL
STDVIEASAQAFASAYNAIYRSLKVEERKMA
>Mature_511_residues
MKDNKIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQKIASKVKNSTICALSRALDK
DIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEILSMAKRAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAI
KAGAKTINIPDTVGYTLPSEFANIIKILFNKVPNIDKAIISVHCHNDLGMATGNSLSAILQGARQIECTINGLGERAGNC
ALEEVVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSGIHQDGVLKNRQTYEIISPSA
IGIHDNRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYERFLRLADKKGQVYDYDLEALMFLSYENEEENEFVIEKLSV
ISGNIPTACVCMRIKEELKTEACTGNGPVEAVFNCIARITNLKPVLKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGL
STDVIEASAQAFASAYNAIYRSLKVEERKMA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=505, Percent_Identity=59.6039603960396, Blast_Score=620, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6320019, Length=365, Percent_Identity=29.5890410958904, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=361, Percent_Identity=29.3628808864266, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=547, Percent_Identity=25.0457038391225, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=564, Percent_Identity=26.241134751773, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56368; Mature: 56368

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNKIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQ
CCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEEHHHCCCCEEECCCCCCCCCCHHHHH
KIASKVKNSTICALSRALDKDIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEIL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHH
SMAKRAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAIKAGAKTINIPDTVGYTLPSE
HHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHH
FANIIKILFNKVPNIDKAIISVHCHNDLGMATGNSLSAILQGARQIECTINGLGERAGNC
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEECHHCCCCCH
ALEEVVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
IHQDGVLKNRQTYEIISPSAIGIHDNRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYE
CCCCCCCCCCCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHH
RFLRLADKKGQVYDYDLEALMFLSYENEEENEFVIEKLSVISGNIPTACVCMRIKEELKT
HHHHHHCCCCCEEEECHHEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EACTGNGPVEAVFNCIARITNLKPVLKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGL
HCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEECCCC
STDVIEASAQAFASAYNAIYRSLKVEERKMA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDNKIIIFDTTLRDGEQALGSSLGINQKLQIALALENLGVDVIEAGFPVSSQGDFKAVQ
CCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEEHHHCCCCEEECCCCCCCCCCHHHHH
KIASKVKNSTICALSRALDKDIDMAYEALKVAKHFRIHTFIATSTLHMQDKLKKDFDEIL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHH
SMAKRAIIRARSYTDDVEFSCEDAGRTPIDNLCFMVENAIKAGAKTINIPDTVGYTLPSE
HHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHH
FANIIKILFNKVPNIDKAIISVHCHNDLGMATGNSLSAILQGARQIECTINGLGERAGNC
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEECHHCCCCCH
ALEEVVMAIKTRKDYLKGFYTDIKCENIFKTSKLVSAITNESIPSHKAIVGSNAFSHSSG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
IHQDGVLKNRQTYEIISPSAIGIHDNRMLMTARSGRAMIKTCLENLGYDENTYNLDDVYE
CCCCCCCCCCCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHH
RFLRLADKKGQVYDYDLEALMFLSYENEEENEFVIEKLSVISGNIPTACVCMRIKEELKT
HHHHHHCCCCCEEEECHHEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EACTGNGPVEAVFNCIARITNLKPVLKAYSINAKSSGVDAQGQVDVDLEFKGRKFHGKGL
HCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEECCCC
STDVIEASAQAFASAYNAIYRSLKVEERKMA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA