Click here to switch to the map view.

The map label for this gene is metG

Identifier: 57234814

GI number: 57234814

Start: 368114

End: 369775

Strand: Direct

Name: metG

Synonym: DET0388

Alternate gene names: 57234814

Gene position: 368114-369775 (Clockwise)

Preceding gene: 57234815

Following gene: 57234813

Centisome position: 25.05

GC content: 51.14

Gene sequence:

>1662_bases
ATGAGCGAACGAATATTTATAGGTGTGGCCTGGCCTTACGCCAACAGCCAGTTGCATCTGGGTCATGTTGCCGGCGCTTA
TCTGCCGGCAGATATTTTTGCCCGTTACCACCGCACCCGCGGGGATGAAGTGCTTATGGTATCCGGTTCGGATATGCATG
GCACTCCCATTACCATAAGGGCGGAGCAGGAGGGCATTACGGCTGCCGAGGTGGCAGAGCGTTATCACCGGCTGTTTATG
GCCTCGTGGCCGAAGCTGGGTATAAGCTGGGATTGCTATACCTCAACCGCCACTGCCAACCACGCCCGCACCGCTCAGCA
GATGTTCCTCAGCCTGTATGAAAAGGGTTATATTTATAAAGATACGGTTTGCCAGCCGTTTTGCCCCCACTGCAACCGCT
TTTTGCCTGACCGCTATGTAGAGGGGACTTGCCCCCACTGCAAATATGAAGGCGCCCGCGGTGACCAGTGTGACAACTGC
GGCAAACCCTTAAATGCGGCCGAACTGTTAAATTTCCGCTGTAAAAACTGCGGCAATCCTCCTGAATTCCGTGAGACCGA
GCATTTTTTCCTGAAACTTTCGGCATTTGAAGAAGAACTTACCCACTGGGTGGAAACTAAGACCTACTGGCGTACCAATG
TGCTGAATTTCACCCTGCGTTATTTAAAAGAGGGGCTTAAAGACCGGGCTATTACCCGTGATTTGGACTGGGGCGTGCCG
TTGCCGCTTCCGGGTTATGAGGGCAAACGGCTGTATGTCTGGTTTGAAGCGGTAATCGGTTATCTGTCTGCCAGTATAGA
GTGGGCGGCATCTAAAGGCCAGCCGGATGAATGGCAGAAATACTGGGGCGGAGATACCAAAAGCTACTATTTTATAGGCA
AGGACAATATCCCGTTCCATACTATTATCTGGCCGGCTATGCTGATGGGTAGGGGCGGGCTTGATTTGCCGTATGACGTC
CCCTCAAACGAATACCTTACTATAGAAGCCCAGAAGTTTTCCAAGAGCCGTAATAAAGCAATCTGGGTGGATGATGTTCT
TTCACGCTACAGTGTGGATACCCTGCGTTACCTTCTTTCGGCTAATATGCCTGAAAGTTCTGATATGGATTTCTCCTGGA
GGGAATTTGTCCGCCGCAATAATGACGAACTGGTAGCCACTTACGGCAATCTGGCCCAGCGCGTTTTGACTATGGTCTGC
CGTAATTTTGACAACAAAGTACCCGAATACGGTGAGCTGGACGAACGTTCCCTGAACCTTATTGAGAAAACCAGCGCCAT
GCTGTTTGAAACAGACAAGGCTTTGCACGGCTGTAATTTCCGTGAGGCTATTAAACTGGCTATGTCACTGGCCCAGGAGG
CAAACCGCTATCTGGATGAAAAAGCCCCCTGGAAAGAAATAAAGGTGGACAAAGCTGCCGCTGCCCGCAGTTTATACGTG
GCCATGGCGGCTCTTTCCGGGCTTCGGGTGGCTTTTTATCCGTTTTTGCCGGAAAGTTCAAGCAGGCTCAGTACTTACCT
GGGTTTCGGCTCTGAAATAGAAAAAGACGGCTGGGTGCTTAAAATGCCGGTAGCCGGGCAGGAAATGATACCGCCGGAGC
CGCTTTTCAAGAAACTGGAAGATTCGGTTGTAGAAGAAGAAACCGCCCGCATGGGGCTTTAG

Upstream 100 bases:

>100_bases
CCGTTCATTACTGTGTGGAATGCGCCCTGAAGAAAGGGTATGCCAGCTACAAAGAAGAAAAAGGCGAGAGAATTCTAACC
TTTCTGCCCTGAGGATATAA

Downstream 100 bases:

>100_bases
TATTTACCAGGAGGCAAGGTCTTGTTGAAACTTAGCTCTATCTACGCACCTATAAATGAGGGCCTCAAAGGGGTTGAGGA
CGAGTTTAAGCTGGTATCCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSERIFIGVAWPYANSQLHLGHVAGAYLPADIFARYHRTRGDEVLMVSGSDMHGTPITIRAEQEGITAAEVAERYHRLFM
ASWPKLGISWDCYTSTATANHARTAQQMFLSLYEKGYIYKDTVCQPFCPHCNRFLPDRYVEGTCPHCKYEGARGDQCDNC
GKPLNAAELLNFRCKNCGNPPEFRETEHFFLKLSAFEEELTHWVETKTYWRTNVLNFTLRYLKEGLKDRAITRDLDWGVP
LPLPGYEGKRLYVWFEAVIGYLSASIEWAASKGQPDEWQKYWGGDTKSYYFIGKDNIPFHTIIWPAMLMGRGGLDLPYDV
PSNEYLTIEAQKFSKSRNKAIWVDDVLSRYSVDTLRYLLSANMPESSDMDFSWREFVRRNNDELVATYGNLAQRVLTMVC
RNFDNKVPEYGELDERSLNLIEKTSAMLFETDKALHGCNFREAIKLAMSLAQEANRYLDEKAPWKEIKVDKAAAARSLYV
AMAALSGLRVAFYPFLPESSSRLSTYLGFGSEIEKDGWVLKMPVAGQEMIPPEPLFKKLEDSVVEEETARMGL

Sequences:

>Translated_553_residues
MSERIFIGVAWPYANSQLHLGHVAGAYLPADIFARYHRTRGDEVLMVSGSDMHGTPITIRAEQEGITAAEVAERYHRLFM
ASWPKLGISWDCYTSTATANHARTAQQMFLSLYEKGYIYKDTVCQPFCPHCNRFLPDRYVEGTCPHCKYEGARGDQCDNC
GKPLNAAELLNFRCKNCGNPPEFRETEHFFLKLSAFEEELTHWVETKTYWRTNVLNFTLRYLKEGLKDRAITRDLDWGVP
LPLPGYEGKRLYVWFEAVIGYLSASIEWAASKGQPDEWQKYWGGDTKSYYFIGKDNIPFHTIIWPAMLMGRGGLDLPYDV
PSNEYLTIEAQKFSKSRNKAIWVDDVLSRYSVDTLRYLLSANMPESSDMDFSWREFVRRNNDELVATYGNLAQRVLTMVC
RNFDNKVPEYGELDERSLNLIEKTSAMLFETDKALHGCNFREAIKLAMSLAQEANRYLDEKAPWKEIKVDKAAAARSLYV
AMAALSGLRVAFYPFLPESSSRLSTYLGFGSEIEKDGWVLKMPVAGQEMIPPEPLFKKLEDSVVEEETARMGL
>Mature_552_residues
SERIFIGVAWPYANSQLHLGHVAGAYLPADIFARYHRTRGDEVLMVSGSDMHGTPITIRAEQEGITAAEVAERYHRLFMA
SWPKLGISWDCYTSTATANHARTAQQMFLSLYEKGYIYKDTVCQPFCPHCNRFLPDRYVEGTCPHCKYEGARGDQCDNCG
KPLNAAELLNFRCKNCGNPPEFRETEHFFLKLSAFEEELTHWVETKTYWRTNVLNFTLRYLKEGLKDRAITRDLDWGVPL
PLPGYEGKRLYVWFEAVIGYLSASIEWAASKGQPDEWQKYWGGDTKSYYFIGKDNIPFHTIIWPAMLMGRGGLDLPYDVP
SNEYLTIEAQKFSKSRNKAIWVDDVLSRYSVDTLRYLLSANMPESSDMDFSWREFVRRNNDELVATYGNLAQRVLTMVCR
NFDNKVPEYGELDERSLNLIEKTSAMLFETDKALHGCNFREAIKLAMSLAQEANRYLDEKAPWKEIKVDKAAAARSLYVA
MAALSGLRVAFYPFLPESSSRLSTYLGFGSEIEKDGWVLKMPVAGQEMIPPEPLFKKLEDSVVEEETARMGL

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=563, Percent_Identity=35.5239786856128, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI24308436, Length=538, Percent_Identity=26.7657992565056, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788432, Length=550, Percent_Identity=31.6363636363636, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1786861, Length=208, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=560, Percent_Identity=33.9285714285714, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI212645096, Length=519, Percent_Identity=25.2408477842004, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=420, Percent_Identity=25, Blast_Score=112, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=34.0425531914894, Blast_Score=342, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6321610, Length=536, Percent_Identity=22.5746268656716, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655311, Length=565, Percent_Identity=35.3982300884956, Blast_Score=355, Evalue=5e-98,
Organism=Drosophila melanogaster, GI21358483, Length=514, Percent_Identity=26.4591439688716, Blast_Score=152, Evalue=8e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_DEHE1 (Q3Z9G6)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181133.1
- ProteinModelPortal:   Q3Z9G6
- STRING:   Q3Z9G6
- GeneID:   3230283
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0388
- NMPDR:   fig|243164.3.peg.525
- TIGR:   DET0388
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   WGIWIDE
- PhylomeDB:   Q3Z9G6
- ProtClustDB:   PRK12268
- BioCyc:   DETH243164:DET_0388-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 63317; Mature: 63185

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERIFIGVAWPYANSQLHLGHVAGAYLPADIFARYHRTRGDEVLMVSGSDMHGTPITIR
CCCEEEEEEECCCCCCEEEEHHHHCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
AEQEGITAAEVAERYHRLFMASWPKLGISWDCYTSTATANHARTAQQMFLSLYEKGYIYK
ECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEE
DTVCQPFCPHCNRFLPDRYVEGTCPHCKYEGARGDQCDNCGKPLNAAELLNFRCKNCGNP
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PEFRETEHFFLKLSAFEEELTHWVETKTYWRTNVLNFTLRYLKEGLKDRAITRDLDWGVP
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LPLPGYEGKRLYVWFEAVIGYLSASIEWAASKGQPDEWQKYWGGDTKSYYFIGKDNIPFH
CCCCCCCCCEEEEEHHHHHHHHHCCHHEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCH
TIIWPAMLMGRGGLDLPYDVPSNEYLTIEAQKFSKSRNKAIWVDDVLSRYSVDTLRYLLS
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
ANMPESSDMDFSWREFVRRNNDELVATYGNLAQRVLTMVCRNFDNKVPEYGELDERSLNL
CCCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
IEKTSAMLFETDKALHGCNFREAIKLAMSLAQEANRYLDEKAPWKEIKVDKAAAARSLYV
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHH
AMAALSGLRVAFYPFLPESSSRLSTYLGFGSEIEKDGWVLKMPVAGQEMIPPEPLFKKLE
HHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
DSVVEEETARMGL
HHHHHHHHHHCCC
>Mature Secondary Structure 
SERIFIGVAWPYANSQLHLGHVAGAYLPADIFARYHRTRGDEVLMVSGSDMHGTPITIR
CCEEEEEEECCCCCCEEEEHHHHCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
AEQEGITAAEVAERYHRLFMASWPKLGISWDCYTSTATANHARTAQQMFLSLYEKGYIYK
ECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEE
DTVCQPFCPHCNRFLPDRYVEGTCPHCKYEGARGDQCDNCGKPLNAAELLNFRCKNCGNP
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
PEFRETEHFFLKLSAFEEELTHWVETKTYWRTNVLNFTLRYLKEGLKDRAITRDLDWGVP
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LPLPGYEGKRLYVWFEAVIGYLSASIEWAASKGQPDEWQKYWGGDTKSYYFIGKDNIPFH
CCCCCCCCCEEEEEHHHHHHHHHCCHHEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCH
TIIWPAMLMGRGGLDLPYDVPSNEYLTIEAQKFSKSRNKAIWVDDVLSRYSVDTLRYLLS
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHH
ANMPESSDMDFSWREFVRRNNDELVATYGNLAQRVLTMVCRNFDNKVPEYGELDERSLNL
CCCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
IEKTSAMLFETDKALHGCNFREAIKLAMSLAQEANRYLDEKAPWKEIKVDKAAAARSLYV
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHH
AMAALSGLRVAFYPFLPESSSRLSTYLGFGSEIEKDGWVLKMPVAGQEMIPPEPLFKKLE
HHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
DSVVEEETARMGL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA