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The map label for this gene is rnjA [H]

Identifier: 57234760

GI number: 57234760

Start: 414099

End: 415754

Strand: Direct

Name: rnjA [H]

Synonym: DET0438

Alternate gene names: 57234760

Gene position: 414099-415754 (Clockwise)

Preceding gene: 57234761

Following gene: 57234759

Centisome position: 28.18

GC content: 50.6

Gene sequence:

>1656_bases
ATGGCAAAACCTAAACTTAAGATTGTACCTCTGGGTGGCCTGGGTGAGATAGGCAAGAATATGATGTCTGTAGAATATGG
GGAGGATATTATCCTCATAGACTGCGGGCTGATGTTCCCCGAAGAAGAAATGCTGGGTATAGATTTGGTTATACCTGACA
TTTCATACGTGCTGGAAAACCGCGAGAAAGTACGGGGTATTGTAATCACCCACGGGCATGAAGACCATATAGGGGCGTTA
CCTTATATACTTCCCCAGATAAATGTGCCTATTTATTGCACCAAACTAACACAGGGGCTTATTTCGGTAAAACTGAAAGA
AGCCAAGCTGCTGCACCAGACTAAGATAAATGTTATCCCGTCTGACGGCACTTTCAAGCTGGGTAAACTCAAAGTGGACT
TTTACCCGGTCTGCCACAGCCTGCCGGATTCGGTGGGGCTGGTTATTCAGACGCCTATCGGGGCGGTAGTCCACAGCGGG
GATTTCAAACTGGACTATACGCCGGTAGACGGCAAGCCCACCAATCTGTCACGTCTGGCTATGCTGGGCTCGCGTGGAGT
ACTGCTTTTGATGTCAGATTCCACCCATGTGGAGCTGCCGGGTTATACCCCTTCGGAAACTGTGGTGGGTGAAAATATTG
ACCGCATTATCGGGGCGGCTACGGGGCGGGTACTGGTAACCACTTTTGCTTCGCTTATTTCCCGCCAGCAGCAGGTGATT
GATGCCGCCGCCAAATACGGGCGGAAGCTCTTTTTTGCCGGCCGCAGCATGACTGAGATTCATAAAATGGCGGCTGATTT
GGGTTACCTTAAAGTGCCCGAAGGTCTGGTCTGCAATATAGACCAGCTTCAGCGTGTGCCTCCGGAAAAAGTGGTTTTGA
TGACCACCGGTTCTCAGGGTGAGCCTACTTCCGCTCTGGTGCGGATAGCCAACCGTGATTTCCGCCATGTCCATATAATG
AAAGGTGACACCGTAATTATTTCGGCTTCGCCCATACCCGGCAACGAGTCACTGGTGAGCCGTACTATAGACAGTTTGTT
CCGCCAGGGGGCTAAGGTATTTTATGACCGCATAGCCAAGGTGCATGTGCACGGGCATGCCAGCCAGGAAGAACTGAAGC
TGGTGCTTAATCTGGTTAAGCCCAAGTATTTTGTACCCGTACATGGCGAATACCGCCACCTGACCATGCATGCGGAGCTG
GCCCGGACTATGGGCGTTGCCCCGGAGAATACTTTTATCATGGAAGACGGGGATATACTGGAACTCAATACCAAGTCCGG
CCGTATTACCGGCAAAGTTCCTTCCGGCAATGTTTATGTAGACGGCCTGAGCGTGGGTGATGTGGGCGGGGTGGTACTCC
GCAACCGCCGTATGCTTTCGCAGGACGGTATTGTGGTGGCGATTGTAGCTATCAACAAGCAAACCGGTATGCTGGTAGGC
AGGCCGGATATTGTTTCCAGAGGTTTTGTTGATCCTGATGAATCTAAAGCCATGCTTGATGCCAGCCGTGATCTTATAAT
CAAGCTGCTTGACCATGACGGTAAGGATATTCCCGAAGGGGCTATGTATAATGACGTCAAAGAAACGCTGAACAAGTTTT
ACTACGAGCAGACTAAACGGCGTCCCATGGTTATCCCGGTTATGGTAAAAGTGTAG

Upstream 100 bases:

>100_bases
GCTCCGGATACCGGGGTTGCTTGAAGAACTGAACCGCGCTGAAGCGCCTTTAGTCCCCAAAGAACCTCCTGCCCGCCAGC
TAGGCCTGTTCTGACGAACT

Downstream 100 bases:

>100_bases
GTTTGCAGACGAGGTTAATATGCCTAAAAAAGAAGACCCCAGAGACACCCTGCGGAATGCCCGCCCCAGAGCTAAATCTG
CGGTTAAGGTAACTGCCCGT

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MAKPKLKIVPLGGLGEIGKNMMSVEYGEDIILIDCGLMFPEEEMLGIDLVIPDISYVLENREKVRGIVITHGHEDHIGAL
PYILPQINVPIYCTKLTQGLISVKLKEAKLLHQTKINVIPSDGTFKLGKLKVDFYPVCHSLPDSVGLVIQTPIGAVVHSG
DFKLDYTPVDGKPTNLSRLAMLGSRGVLLLMSDSTHVELPGYTPSETVVGENIDRIIGAATGRVLVTTFASLISRQQQVI
DAAAKYGRKLFFAGRSMTEIHKMAADLGYLKVPEGLVCNIDQLQRVPPEKVVLMTTGSQGEPTSALVRIANRDFRHVHIM
KGDTVIISASPIPGNESLVSRTIDSLFRQGAKVFYDRIAKVHVHGHASQEELKLVLNLVKPKYFVPVHGEYRHLTMHAEL
ARTMGVAPENTFIMEDGDILELNTKSGRITGKVPSGNVYVDGLSVGDVGGVVLRNRRMLSQDGIVVAIVAINKQTGMLVG
RPDIVSRGFVDPDESKAMLDASRDLIIKLLDHDGKDIPEGAMYNDVKETLNKFYYEQTKRRPMVIPVMVKV

Sequences:

>Translated_551_residues
MAKPKLKIVPLGGLGEIGKNMMSVEYGEDIILIDCGLMFPEEEMLGIDLVIPDISYVLENREKVRGIVITHGHEDHIGAL
PYILPQINVPIYCTKLTQGLISVKLKEAKLLHQTKINVIPSDGTFKLGKLKVDFYPVCHSLPDSVGLVIQTPIGAVVHSG
DFKLDYTPVDGKPTNLSRLAMLGSRGVLLLMSDSTHVELPGYTPSETVVGENIDRIIGAATGRVLVTTFASLISRQQQVI
DAAAKYGRKLFFAGRSMTEIHKMAADLGYLKVPEGLVCNIDQLQRVPPEKVVLMTTGSQGEPTSALVRIANRDFRHVHIM
KGDTVIISASPIPGNESLVSRTIDSLFRQGAKVFYDRIAKVHVHGHASQEELKLVLNLVKPKYFVPVHGEYRHLTMHAEL
ARTMGVAPENTFIMEDGDILELNTKSGRITGKVPSGNVYVDGLSVGDVGGVVLRNRRMLSQDGIVVAIVAINKQTGMLVG
RPDIVSRGFVDPDESKAMLDASRDLIIKLLDHDGKDIPEGAMYNDVKETLNKFYYEQTKRRPMVIPVMVKV
>Mature_550_residues
AKPKLKIVPLGGLGEIGKNMMSVEYGEDIILIDCGLMFPEEEMLGIDLVIPDISYVLENREKVRGIVITHGHEDHIGALP
YILPQINVPIYCTKLTQGLISVKLKEAKLLHQTKINVIPSDGTFKLGKLKVDFYPVCHSLPDSVGLVIQTPIGAVVHSGD
FKLDYTPVDGKPTNLSRLAMLGSRGVLLLMSDSTHVELPGYTPSETVVGENIDRIIGAATGRVLVTTFASLISRQQQVID
AAAKYGRKLFFAGRSMTEIHKMAADLGYLKVPEGLVCNIDQLQRVPPEKVVLMTTGSQGEPTSALVRIANRDFRHVHIMK
GDTVIISASPIPGNESLVSRTIDSLFRQGAKVFYDRIAKVHVHGHASQEELKLVLNLVKPKYFVPVHGEYRHLTMHAELA
RTMGVAPENTFIMEDGDILELNTKSGRITGKVPSGNVYVDGLSVGDVGGVVLRNRRMLSQDGIVVAIVAINKQTGMLVGR
PDIVSRGFVDPDESKAMLDASRDLIIKLLDHDGKDIPEGAMYNDVKETLNKFYYEQTKRRPMVIPVMVKV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 60504; Mature: 60372

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPKLKIVPLGGLGEIGKNMMSVEYGEDIILIDCGLMFPEEEMLGIDLVIPDISYVLEN
CCCCCEEEEEECCCHHHCCCCEEEECCCCEEEEECCCCCCCHHHEEEEEEECCHHHHHCC
REKVRGIVITHGHEDHIGALPYILPQINVPIYCTKLTQGLISVKLKEAKLLHQTKINVIP
CCCEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEHHHCCHHEEEEHHHHHHHHEEEEEEE
SDGTFKLGKLKVDFYPVCHSLPDSVGLVIQTPIGAVVHSGDFKLDYTPVDGKPTNLSRLA
CCCCEEEEEEEEEEEHHHHCCCCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCHHHHE
MLGSRGVLLLMSDSTHVELPGYTPSETVVGENIDRIIGAATGRVLVTTFASLISRQQQVI
EECCCCEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DAAAKYGRKLFFAGRSMTEIHKMAADLGYLKVPEGLVCNIDQLQRVPPEKVVLMTTGSQG
HHHHHCCCEEEECCCCHHHHHHHHHHCCCEECCCCCEECHHHHHCCCCCCEEEEEECCCC
EPTSALVRIANRDFRHVHIMKGDTVIISASPIPGNESLVSRTIDSLFRQGAKVFYDRIAK
CCHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
VHVHGHASQEELKLVLNLVKPKYFVPVHGEYRHLTMHAELARTMGVAPENTFIMEDGDIL
EEEECCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHHHHCCCCCCEEEEECCCEE
ELNTKSGRITGKVPSGNVYVDGLSVGDVGGVVLRNRRMLSQDGIVVAIVAINKQTGMLVG
EEECCCCEEEEECCCCCEEEEEEEECCCCCEEEECCEEECCCCEEEEEEEECCCCCEEEC
RPDIVSRGFVDPDESKAMLDASRDLIIKLLDHDGKDIPEGAMYNDVKETLNKFYYEQTKR
CCCHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
RPMVIPVMVKV
CCEEEEEEEEC
>Mature Secondary Structure 
AKPKLKIVPLGGLGEIGKNMMSVEYGEDIILIDCGLMFPEEEMLGIDLVIPDISYVLEN
CCCCEEEEEECCCHHHCCCCEEEECCCCEEEEECCCCCCCHHHEEEEEEECCHHHHHCC
REKVRGIVITHGHEDHIGALPYILPQINVPIYCTKLTQGLISVKLKEAKLLHQTKINVIP
CCCEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEHHHCCHHEEEEHHHHHHHHEEEEEEE
SDGTFKLGKLKVDFYPVCHSLPDSVGLVIQTPIGAVVHSGDFKLDYTPVDGKPTNLSRLA
CCCCEEEEEEEEEEEHHHHCCCCCCCEEEECCCCEEEECCCCEEEEECCCCCCCCHHHHE
MLGSRGVLLLMSDSTHVELPGYTPSETVVGENIDRIIGAATGRVLVTTFASLISRQQQVI
EECCCCEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DAAAKYGRKLFFAGRSMTEIHKMAADLGYLKVPEGLVCNIDQLQRVPPEKVVLMTTGSQG
HHHHHCCCEEEECCCCHHHHHHHHHHCCCEECCCCCEECHHHHHCCCCCCEEEEEECCCC
EPTSALVRIANRDFRHVHIMKGDTVIISASPIPGNESLVSRTIDSLFRQGAKVFYDRIAK
CCHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
VHVHGHASQEELKLVLNLVKPKYFVPVHGEYRHLTMHAELARTMGVAPENTFIMEDGDIL
EEEECCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHHHHCCCCCCEEEEECCCEE
ELNTKSGRITGKVPSGNVYVDGLSVGDVGGVVLRNRRMLSQDGIVVAIVAINKQTGMLVG
EEECCCCEEEEECCCCCEEEEEEEECCCCCEEEECCEEECCCCEEEEEEEECCCCCEEEC
RPDIVSRGFVDPDESKAMLDASRDLIIKLLDHDGKDIPEGAMYNDVKETLNKFYYEQTKR
CCCHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
RPMVIPVMVKV
CCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]