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The map label for this gene is leuC [H]

Identifier: 57234733

GI number: 57234733

Start: 425080

End: 426333

Strand: Direct

Name: leuC [H]

Synonym: DET0448

Alternate gene names: 57234733

Gene position: 425080-426333 (Clockwise)

Preceding gene: 57234734

Following gene: 57234732

Centisome position: 28.92

GC content: 53.91

Gene sequence:

>1254_bases
ATGGGGAAAACACTAGCAGAAAAAATACTCAGCCTGAAGTCTGGCAGTGATGCCAGCGCCGGAGACATAGTAGTCTCCAA
GGTGGACCTGGCCTTTGTTCAGGATACCACCGGTCCTCTGACTGTCAGGGAATTTTGGGACAACGGCTTTACCAAACTGG
CTAATCCGTCACGGACAGCCCTTTTTCTGGACCATGCCGCACCCAGCCCCCAAAGACAGCTTTCAACAGACCATATCCTG
CTTCGCAAGTTTGCCCGGGATACCGGGGCGCTCATTTTTGATGTAGGCGAGGGTGTCTGTCACCAGCTGGTAGCCGAAAA
GCTGGCCAGACCGGGTGATGTGATTGTCGGGGCGGATTCCCATACGGTTACCGCCGGCGGTCTGGGCGCTTTTTCCACCG
GCATGGGGTCATCTGATATTGCGGTGGCCTTTGCCCTGGGCAAGACCTGGTTCCGGGTGCCGGAGACTATTAAAGTAGTA
GTCAGCGGGCGTTTTAAGCACGGGATTTATGCCAAAGACCTTATTTTGTATCTTATCGGGCTTATCGGGGCGGACGGGGC
TACCTATAAAGCTCTGGAGTTCTCCGGTAATGTGGTGAATAATATGACGATAGCTGAAAGGCTGACTATCGCCAATATGG
CGGTTGAGGCCGGGGCTAAAGTAGGGCTTTTCCCATCTGACCGCCAGACACTTGAATACCTGCGTTCGGTAGGGCGTGAG
GCTGACTACCAGCCTTTGGCGGCTGATGAAGATGCAGTGTATGAACGGGTGATAGAAATAGACGCAACCGCCCTTGAACC
TATGGTTGCCAAACCCCACACTGTAGACAATACCGCTACTGCCCGCGAGCTGAAAGGTACTAAATTAGACCAGGTGTTTA
TCGGTACCTGTACCGGGGGGCGTCTGGATGATTTGGCGGTGGCAGCGGCTATATTTAAGAACCGCAGACACCATCCCCAA
ACCCGCCTGATAGTCACGCCTGCTTCTCAGAAAGTATATCTGGAAGCTATCCGCCTGGGATATATAGAAATACTGGTGCA
GGCCGGGGCGAATGTTATGCCGCCCGGCTGCGGTGCCTGTCTGGGTGTCCATCAGGGTGTTTTGGGAGACGGCGAAGTCT
GTCTTTCCACTGCCAACCGCAATTTCAAAGGCCGGATGGGCAACCCCGAAGGGTTTATTTATCTGGCAAGTGCGGCAACG
GCTGCGGCATCAGCCATCAAGGGTGAAATTTCAGACCCCAGAGAGGTAATGTAA

Upstream 100 bases:

>100_bases
ATAATAAAAGCCCTGCGCTCTCTGGAAAACAAGGTGCTGGCAAATCCTCCTAAGAAACACGGGAATATACCTTTGTAAAG
GAAGGTTGCATAAACAAAAC

Downstream 100 bases:

>100_bases
TGCTTAAGGGCAAAGCTTTTAAGTTTGGTGATAGCATATCCACTGACCATATTGCACCCGGCCGTCTGGTGCACCTGCGG
AGCAATCTGCCGGAACTGGC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGKTLAEKILSLKSGSDASAGDIVVSKVDLAFVQDTTGPLTVREFWDNGFTKLANPSRTALFLDHAAPSPQRQLSTDHIL
LRKFARDTGALIFDVGEGVCHQLVAEKLARPGDVIVGADSHTVTAGGLGAFSTGMGSSDIAVAFALGKTWFRVPETIKVV
VSGRFKHGIYAKDLILYLIGLIGADGATYKALEFSGNVVNNMTIAERLTIANMAVEAGAKVGLFPSDRQTLEYLRSVGRE
ADYQPLAADEDAVYERVIEIDATALEPMVAKPHTVDNTATARELKGTKLDQVFIGTCTGGRLDDLAVAAAIFKNRRHHPQ
TRLIVTPASQKVYLEAIRLGYIEILVQAGANVMPPGCGACLGVHQGVLGDGEVCLSTANRNFKGRMGNPEGFIYLASAAT
AAASAIKGEISDPREVM

Sequences:

>Translated_417_residues
MGKTLAEKILSLKSGSDASAGDIVVSKVDLAFVQDTTGPLTVREFWDNGFTKLANPSRTALFLDHAAPSPQRQLSTDHIL
LRKFARDTGALIFDVGEGVCHQLVAEKLARPGDVIVGADSHTVTAGGLGAFSTGMGSSDIAVAFALGKTWFRVPETIKVV
VSGRFKHGIYAKDLILYLIGLIGADGATYKALEFSGNVVNNMTIAERLTIANMAVEAGAKVGLFPSDRQTLEYLRSVGRE
ADYQPLAADEDAVYERVIEIDATALEPMVAKPHTVDNTATARELKGTKLDQVFIGTCTGGRLDDLAVAAAIFKNRRHHPQ
TRLIVTPASQKVYLEAIRLGYIEILVQAGANVMPPGCGACLGVHQGVLGDGEVCLSTANRNFKGRMGNPEGFIYLASAAT
AAASAIKGEISDPREVM
>Mature_416_residues
GKTLAEKILSLKSGSDASAGDIVVSKVDLAFVQDTTGPLTVREFWDNGFTKLANPSRTALFLDHAAPSPQRQLSTDHILL
RKFARDTGALIFDVGEGVCHQLVAEKLARPGDVIVGADSHTVTAGGLGAFSTGMGSSDIAVAFALGKTWFRVPETIKVVV
SGRFKHGIYAKDLILYLIGLIGADGATYKALEFSGNVVNNMTIAERLTIANMAVEAGAKVGLFPSDRQTLEYLRSVGREA
DYQPLAADEDAVYERVIEIDATALEPMVAKPHTVDNTATARELKGTKLDQVFIGTCTGGRLDDLAVAAAIFKNRRHHPQT
RLIVTPASQKVYLEAIRLGYIEILVQAGANVMPPGCGACLGVHQGVLGDGEVCLSTANRNFKGRMGNPEGFIYLASAATA
AASAIKGEISDPREVM

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=422, Percent_Identity=29.3838862559242, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI8659555, Length=403, Percent_Identity=25.8064516129032, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=24.0506329113924, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=30.3418803418803, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI2367097, Length=427, Percent_Identity=28.3372365339578, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081781, Length=348, Percent_Identity=26.1494252873563, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787531, Length=393, Percent_Identity=24.9363867684478, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=418, Percent_Identity=29.1866028708134, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=412, Percent_Identity=29.3689320388349, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.9295774647887, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=404, Percent_Identity=26.980198019802, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320440, Length=449, Percent_Identity=34.075723830735, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6323335, Length=451, Percent_Identity=31.2638580931264, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321429, Length=444, Percent_Identity=29.5045045045045, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6322261, Length=358, Percent_Identity=30.1675977653631, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI28571643, Length=422, Percent_Identity=29.6208530805687, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI281365315, Length=451, Percent_Identity=29.0465631929047, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17864292, Length=451, Percent_Identity=29.0465631929047, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI161076999, Length=416, Percent_Identity=29.3269230769231, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137564, Length=409, Percent_Identity=26.4058679706601, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645686, Length=164, Percent_Identity=35.3658536585366, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44299; Mature: 44168

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTLAEKILSLKSGSDASAGDIVVSKVDLAFVQDTTGPLTVREFWDNGFTKLANPSRTA
CCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEHHHHHCCCCHHCCCCCCEE
LFLDHAAPSPQRQLSTDHILLRKFARDTGALIFDVGEGVCHQLVAEKLARPGDVIVGADS
EEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCC
HTVTAGGLGAFSTGMGSSDIAVAFALGKTWFRVPETIKVVVSGRFKHGIYAKDLILYLIG
CEEEECCCCHHHCCCCCCCEEEEEECCCHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LIGADGATYKALEFSGNVVNNMTIAERLTIANMAVEAGAKVGLFPSDRQTLEYLRSVGRE
HHCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCC
ADYQPLAADEDAVYERVIEIDATALEPMVAKPHTVDNTATARELKGTKLDQVFIGTCTGG
CCCCCCCCCHHHHHHHHHHHCHHHCCCHHCCCCCCCCCHHHHHHCCCEECEEEEEECCCC
RLDDLAVAAAIFKNRRHHPQTRLIVTPASQKVYLEAIRLGYIEILVQAGANVMPPGCGAC
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LGVHQGVLGDGEVCLSTANRNFKGRMGNPEGFIYLASAATAAASAIKGEISDPREVM
HHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCHHHCC
>Mature Secondary Structure 
GKTLAEKILSLKSGSDASAGDIVVSKVDLAFVQDTTGPLTVREFWDNGFTKLANPSRTA
CHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEHHHHHCCCCHHCCCCCCEE
LFLDHAAPSPQRQLSTDHILLRKFARDTGALIFDVGEGVCHQLVAEKLARPGDVIVGADS
EEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCC
HTVTAGGLGAFSTGMGSSDIAVAFALGKTWFRVPETIKVVVSGRFKHGIYAKDLILYLIG
CEEEECCCCHHHCCCCCCCEEEEEECCCHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LIGADGATYKALEFSGNVVNNMTIAERLTIANMAVEAGAKVGLFPSDRQTLEYLRSVGRE
HHCCCCCEEEEEEECCCEECCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCC
ADYQPLAADEDAVYERVIEIDATALEPMVAKPHTVDNTATARELKGTKLDQVFIGTCTGG
CCCCCCCCCHHHHHHHHHHHCHHHCCCHHCCCCCCCCCHHHHHHCCCEECEEEEEECCCC
RLDDLAVAAAIFKNRRHHPQTRLIVTPASQKVYLEAIRLGYIEILVQAGANVMPPGCGAC
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LGVHQGVLGDGEVCLSTANRNFKGRMGNPEGFIYLASAATAAASAIKGEISDPREVM
HHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA