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The map label for this gene is nfi [H]

Identifier: 57234654

GI number: 57234654

Start: 521809

End: 522474

Strand: Direct

Name: nfi [H]

Synonym: DET0569

Alternate gene names: 57234654

Gene position: 521809-522474 (Clockwise)

Preceding gene: 57234666

Following gene: 57234653

Centisome position: 35.5

GC content: 52.7

Gene sequence:

>666_bases
GTGAAAATTAAAAAGCTTCATGATTGGGATATGACGCCCTCTCAGGCAATAGCTCTCCAGCGTGAGCTGGCCCAGAAAGT
GTCTGTTTGCGGTACTCTTCCGGATATACATCTGGTTGCCGGAGCAGATGTCTGGCACAGCCGCACTTCAGGTATGGGCA
GGGCAGCAGTAGTGGTGCTTAGTTATCCCGACATGCAGCTGGTGGAAGTGAGCCGTTCCGAAGGGCATTGCCATTTCCCG
TATATACCCGGCTTGCTCTCATTCCGGGAGATGCCCCTTCTGCTTTCTGCCTTTGAGGGGCTGGAAAATATACCTGATTT
AATTCTGATGGACGGGCAGGGTTTAGCTCACCCCAGGCGGCTGGGTATTGCCTCGCATCTGGGGCTTTTTTTAAATAAAC
CGGTTATCGGTTGCGCCAAAAGCCGTTTGATAGGTGAATATACCGCTCTGGACGAAAGTGCCGGCAGTTTTTCAGATTTG
TATCATAACACAGAGCTGATAGGGCGGGTTATGCGCACCCGCCAGGGGGTAAACCCTTTGTTTATCTCTGTGGGGCACAA
AATTTGCCTTGAGGAAGCCTGCAGTATGGTTGCAGAATGCTGCCGGGGATACCGTCTGCCCGAACCCACCCGTCAGGCTC
ACCTGGCGGCTGCCGAGCTGATTTAA

Upstream 100 bases:

>100_bases
AGCTGTCAAATAAGCCGTTAGTGTTGCTTAGCCCCAGGAAGGTATAAATATATACAAATTATCTCTGTAGTTATCTAAAA
ATATTAGATATAATGTGAAT

Downstream 100 bases:

>100_bases
CCCTCCTTCCGGTATTATCATATCCGCCCATAAATGGTATTCTATATAATGCGGGTTCGGGTATGCAGCCCCTAACCCTA
GTTTGTTTTCAGCGTTATAT

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKIKKLHDWDMTPSQAIALQRELAQKVSVCGTLPDIHLVAGADVWHSRTSGMGRAAVVVLSYPDMQLVEVSRSEGHCHFP
YIPGLLSFREMPLLLSAFEGLENIPDLILMDGQGLAHPRRLGIASHLGLFLNKPVIGCAKSRLIGEYTALDESAGSFSDL
YHNTELIGRVMRTRQGVNPLFISVGHKICLEEACSMVAECCRGYRLPEPTRQAHLAAAELI

Sequences:

>Translated_221_residues
MKIKKLHDWDMTPSQAIALQRELAQKVSVCGTLPDIHLVAGADVWHSRTSGMGRAAVVVLSYPDMQLVEVSRSEGHCHFP
YIPGLLSFREMPLLLSAFEGLENIPDLILMDGQGLAHPRRLGIASHLGLFLNKPVIGCAKSRLIGEYTALDESAGSFSDL
YHNTELIGRVMRTRQGVNPLFISVGHKICLEEACSMVAECCRGYRLPEPTRQAHLAAAELI
>Mature_221_residues
MKIKKLHDWDMTPSQAIALQRELAQKVSVCGTLPDIHLVAGADVWHSRTSGMGRAAVVVLSYPDMQLVEVSRSEGHCHFP
YIPGLLSFREMPLLLSAFEGLENIPDLILMDGQGLAHPRRLGIASHLGLFLNKPVIGCAKSRLIGEYTALDESAGSFSDL
YHNTELIGRVMRTRQGVNPLFISVGHKICLEEACSMVAECCRGYRLPEPTRQAHLAAAELI

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=204, Percent_Identity=33.8235294117647, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI257467548, Length=128, Percent_Identity=35.9375, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI257467550, Length=141, Percent_Identity=35.4609929078014, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI87082357, Length=206, Percent_Identity=43.6893203883495, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71981509, Length=231, Percent_Identity=26.4069264069264, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 24298; Mature: 24298

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKKLHDWDMTPSQAIALQRELAQKVSVCGTLPDIHLVAGADVWHSRTSGMGRAAVVVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCEEEEEE
SYPDMQLVEVSRSEGHCHFPYIPGLLSFREMPLLLSAFEGLENIPDLILMDGQGLAHPRR
ECCCCEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
LGIASHLGLFLNKPVIGCAKSRLIGEYTALDESAGSFSDLYHNTELIGRVMRTRQGVNPL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
FISVGHKICLEEACSMVAECCRGYRLPEPTRQAHLAAAELI
EEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIKKLHDWDMTPSQAIALQRELAQKVSVCGTLPDIHLVAGADVWHSRTSGMGRAAVVVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCEEEEEE
SYPDMQLVEVSRSEGHCHFPYIPGLLSFREMPLLLSAFEGLENIPDLILMDGQGLAHPRR
ECCCCEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
LGIASHLGLFLNKPVIGCAKSRLIGEYTALDESAGSFSDLYHNTELIGRVMRTRQGVNPL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
FISVGHKICLEEACSMVAECCRGYRLPEPTRQAHLAAAELI
EEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA