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The map label for this gene is nuoE [H]

Identifier: 57234507

GI number: 57234507

Start: 671263

End: 671742

Strand: Direct

Name: nuoE [H]

Synonym: DET0728

Alternate gene names: 57234507

Gene position: 671263-671742 (Clockwise)

Preceding gene: 57234508

Following gene: 57234506

Centisome position: 45.67

GC content: 47.5

Gene sequence:

>480_bases
ATGTGCACCGAAACTAGAGAAATAGTAGCGCCAAAAGTTAAAAATATTCTGGATAAATATGCCAAAGACAAGGGTATGCT
GGTAGCTATACTGCAGGATATCCAGACTGAGTTCAACTATCTTCCCCGTCCCGCTCTGGAGACCGTAAGCGAGGGCTTGG
GTGTTCCCATGAGCCAGGTTTACAGTGTGGCCACTTTCTTCAAGGCTTTCAGCCTGAAACCCAAGGGCAAACACTCTATT
CATGTATGTATGGGTACTGCCTGCCACGTACGCGGTGCTAATAAGATACTGGATAAACTGGTTGAAAAACTGGGTTGTTG
TGCCGGTGAAAATACCGCAGACATGAAATTCAGCCTTGATGCCGTAAACTGCGTGGGCGCCTGTGCTTTGGGGCCTGTGG
TGGTAGTGGACGGGCAGTATGTAGGCAATATGACTACTGAAAAGGTCAAACCTTTGATTGAGGGATGCCAGGATGATTAA

Upstream 100 bases:

>100_bases
CCAGTCTTCAGCGGCGGTTCCGCCACCTGACAGAAGAATCTCTGGACCTTATTCAGGAAAGAGTTCAGCTGGAATACAAT
AAGATTGCAGAGAGGGCGGC

Downstream 100 bases:

>100_bases
TACAGCAATTAAAAAGTTTAACAGTGTAGCCGAACTTGAGGCCAAACGGCAGGAAATCCTTAAAGACAGGAATTCCCAAA
AGACGGTTATTGCTATTTGC

Product: [Fe] hydrogenase, HymA subunit, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MCTETREIVAPKVKNILDKYAKDKGMLVAILQDIQTEFNYLPRPALETVSEGLGVPMSQVYSVATFFKAFSLKPKGKHSI
HVCMGTACHVRGANKILDKLVEKLGCCAGENTADMKFSLDAVNCVGACALGPVVVVDGQYVGNMTTEKVKPLIEGCQDD

Sequences:

>Translated_159_residues
MCTETREIVAPKVKNILDKYAKDKGMLVAILQDIQTEFNYLPRPALETVSEGLGVPMSQVYSVATFFKAFSLKPKGKHSI
HVCMGTACHVRGANKILDKLVEKLGCCAGENTADMKFSLDAVNCVGACALGPVVVVDGQYVGNMTTEKVKPLIEGCQDD
>Mature_159_residues
MCTETREIVAPKVKNILDKYAKDKGMLVAILQDIQTEFNYLPRPALETVSEGLGVPMSQVYSVATFFKAFSLKPKGKHSI
HVCMGTACHVRGANKILDKLVEKLGCCAGENTADMKFSLDAVNCVGACALGPVVVVDGQYVGNMTTEKVKPLIEGCQDD

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=145, Percent_Identity=33.7931034482759, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788621, Length=155, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17561328, Length=145, Percent_Identity=35.1724137931034, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18859877, Length=150, Percent_Identity=34, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21355353, Length=150, Percent_Identity=28.6666666666667, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17170; Mature: 17170

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTETREIVAPKVKNILDKYAKDKGMLVAILQDIQTEFNYLPRPALETVSEGLGVPMSQV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
YSVATFFKAFSLKPKGKHSIHVCMGTACHVRGANKILDKLVEKLGCCAGENTADMKFSLD
HHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECH
AVNCVGACALGPVVVVDGQYVGNMTTEKVKPLIEGCQDD
HHHHHHHHHCCCEEEECCCHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MCTETREIVAPKVKNILDKYAKDKGMLVAILQDIQTEFNYLPRPALETVSEGLGVPMSQV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
YSVATFFKAFSLKPKGKHSIHVCMGTACHVRGANKILDKLVEKLGCCAGENTADMKFSLD
HHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECH
AVNCVGACALGPVVVVDGQYVGNMTTEKVKPLIEGCQDD
HHHHHHHHHCCCEEEECCCHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA