Click here to switch to the map view.

The map label for this gene is gidB [H]

Identifier: 57234428

GI number: 57234428

Start: 713595

End: 714356

Strand: Direct

Name: gidB [H]

Synonym: DET0776

Alternate gene names: 57234428

Gene position: 713595-714356 (Clockwise)

Preceding gene: 57234432

Following gene: 57234427

Centisome position: 48.55

GC content: 47.24

Gene sequence:

>762_bases
TTGTCAAATGCAAAAATAAAGCCTGAAATCTGCCTTTATGCTATATTACTATCCATGAATAAACTTATCTGCGGTCTATC
CTCTTTGGGCATTTCGCTAAGTTCTGTCCAGCTTGAACAGTTTGAAACCTATTACCGTGAACTGGTGGACTATAACAGCC
GGGTTAATCTGACGGCTATAACCGGATACGAAGATATTCAGTTAAAGCACTTTTTAGATTCGGCTTCGCTAGCACTGGCT
GGTATTAAGGGTGATGAAAAACTGCTGGATGTAGGCAGCGGAGCCGGTTTCCCAGGTCTGCCGCTAAAGATAATTTTTCC
AGGTATGAAGCTTGGTTTACTGGAAGCCACTCAGAAAAAGGCCCGCTTTTTAAGCGAAATTACTGCCAAACTGGGTTTAA
CCGGTGTTGAAATAATATCCCAAAGGGCGGAAGATGCCGCCCAAAACCCCCTTTACCGCCAGAAATATACTCTGGTCACC
TCACGGGCAGTGGCGGATATGGCAACTCTCGCCGAACTGACTTTGCCGTTTTGTGCCGTAGGCGGGCGGGTTGTTGCCCC
CAAAAAAGGTGACATAGAGGAAGAGATGGACAGGGCTGCCTCCGCCATTAAAAAGATGGGCGGGCGGGTGTCTGAAATAA
TAAAAGTGGATTTGCCGGGGCTGCAAGACGGCCGTAAACTGGTAGTACTGGAAAAAATATCAAATACCCTGTCCCAGTAT
CCCAGACGGGCGGGAATGCCGGCTAAAAATCCCCTTATTTAG

Upstream 100 bases:

>100_bases
ACGCTCGCATTACCGAGTTCAGGTTTTAGGGTTGGCTATGTGCCTCTCAGCCTGGAGCACCCCTAGCGAGTTTATGTAAA
TAAATGATAACACGGTTAAA

Downstream 100 bases:

>100_bases
CTTGTTGGAGCGCGGTATAAATAGTAAAATATACTAAAGATAGGCAGGGGAAATATTTGTCCGGGTTACTAATTAGATAG
TATGCATATTATTCCGGAGG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSNAKIKPEICLYAILLSMNKLICGLSSLGISLSSVQLEQFETYYRELVDYNSRVNLTAITGYEDIQLKHFLDSASLALA
GIKGDEKLLDVGSGAGFPGLPLKIIFPGMKLGLLEATQKKARFLSEITAKLGLTGVEIISQRAEDAAQNPLYRQKYTLVT
SRAVADMATLAELTLPFCAVGGRVVAPKKGDIEEEMDRAASAIKKMGGRVSEIIKVDLPGLQDGRKLVVLEKISNTLSQY
PRRAGMPAKNPLI

Sequences:

>Translated_253_residues
MSNAKIKPEICLYAILLSMNKLICGLSSLGISLSSVQLEQFETYYRELVDYNSRVNLTAITGYEDIQLKHFLDSASLALA
GIKGDEKLLDVGSGAGFPGLPLKIIFPGMKLGLLEATQKKARFLSEITAKLGLTGVEIISQRAEDAAQNPLYRQKYTLVT
SRAVADMATLAELTLPFCAVGGRVVAPKKGDIEEEMDRAASAIKKMGGRVSEIIKVDLPGLQDGRKLVVLEKISNTLSQY
PRRAGMPAKNPLI
>Mature_252_residues
SNAKIKPEICLYAILLSMNKLICGLSSLGISLSSVQLEQFETYYRELVDYNSRVNLTAITGYEDIQLKHFLDSASLALAG
IKGDEKLLDVGSGAGFPGLPLKIIFPGMKLGLLEATQKKARFLSEITAKLGLTGVEIISQRAEDAAQNPLYRQKYTLVTS
RAVADMATLAELTLPFCAVGGRVVAPKKGDIEEEMDRAASAIKKMGGRVSEIIKVDLPGLQDGRKLVVLEKISNTLSQYP
RRAGMPAKNPLI

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=213, Percent_Identity=30.5164319248826, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.- [C]

Molecular weight: Translated: 27444; Mature: 27312

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAKIKPEICLYAILLSMNKLICGLSSLGISLSSVQLEQFETYYRELVDYNSRVNLTAI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TGYEDIQLKHFLDSASLALAGIKGDEKLLDVGSGAGFPGLPLKIIFPGMKLGLLEATQKK
CCCCHHHHHHHHHHHHHEEECCCCCHHHEECCCCCCCCCCCCEEEECCCHHHHHHHHHHH
ARFLSEITAKLGLTGVEIISQRAEDAAQNPLYRQKYTLVTSRAVADMATLAELTLPFCAV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRVVAPKKGDIEEEMDRAASAIKKMGGRVSEIIKVDLPGLQDGRKLVVLEKISNTLSQY
CCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCEEEEHHHHHHHHHHH
PRRAGMPAKNPLI
HHHCCCCCCCCCC
>Mature Secondary Structure 
SNAKIKPEICLYAILLSMNKLICGLSSLGISLSSVQLEQFETYYRELVDYNSRVNLTAI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TGYEDIQLKHFLDSASLALAGIKGDEKLLDVGSGAGFPGLPLKIIFPGMKLGLLEATQKK
CCCCHHHHHHHHHHHHHEEECCCCCHHHEECCCCCCCCCCCCEEEECCCHHHHHHHHHHH
ARFLSEITAKLGLTGVEIISQRAEDAAQNPLYRQKYTLVTSRAVADMATLAELTLPFCAV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRVVAPKKGDIEEEMDRAASAIKKMGGRVSEIIKVDLPGLQDGRKLVVLEKISNTLSQY
CCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCCCCEEEEHHHHHHHHHHH
PRRAGMPAKNPLI
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA