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The map label for this gene is tmk

Identifier: 57234426

GI number: 57234426

Start: 716102

End: 716725

Strand: Direct

Name: tmk

Synonym: DET0778

Alternate gene names: 57234426

Gene position: 716102-716725 (Clockwise)

Preceding gene: 57234427

Following gene: 57234425

Centisome position: 48.72

GC content: 49.2

Gene sequence:

>624_bases
ATGTCTTTGTTTATAACATTTGAGGGCGGCGAAGGCTGCGGCAAAAGCACCCAGTCCAAGGCATTGTACAGGTATCTGAA
AAAACTGGGGCTGGGGTGTGTGCTTACTCACGAACCCGGCGGTACCCGCTCAGGTGATAAAATAACCCGTTTGTTGAAGT
GGTCTGAAGAAGAAAATATTTCCCCTCTTGCGGAGTTGTTTCTTTTCAACGCCTCGCGTTCTATCCTTATAGACAATGTG
ATTAAACCGGCCTTGCTGGATGGCAATATAGTTATATGTGACCGGTATACAGATTCCACCTTGGCATACCAGGGGTACGG
GCGGGGGCTTGAGCTGGATACGGTAAAATGTATAAACAGCCTGGCAAGCGGCGGTCTGGTACCTGACTTAACCATCTGGC
TGGATATGGATGACAAAGCCGCCCTCCTCCGCAAAGGAAAGTTGCCGCCTGACCGCTTTGAAAGTGAAAATAATGGCTTC
CACCAAAGGGTTCGTGACGGTTTCGGGGTTATTGCCGCTGCCGAACCAAACAGGTTTCTGAAGCTGGATGCTTCACTCTC
CCAGTCAGAGCTTACCAAATGTATTAAGCAAAGGGTTAACGCTCTTTTGAAGCTGCCGCAATGA

Upstream 100 bases:

>100_bases
AAGGCGCTTTCAGCACATAATAGCTGAAAAAGAGCATCTTTCTTCCCATAGTTTGGGCAAAGAGCCGCACCGCCGGGTAA
GAATATCAAGGGGTTAAAAT

Downstream 100 bases:

>100_bases
ATGACACTTCCGAATTAATAATGGTAGCTATGAGCGGCGGGGTGGATTCCTCGGTTGCGGCCATGCTCCTTCTGGAGCAG
GGATACGATGTAGCCGGGGT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSLFITFEGGEGCGKSTQSKALYRYLKKLGLGCVLTHEPGGTRSGDKITRLLKWSEEENISPLAELFLFNASRSILIDNV
IKPALLDGNIVICDRYTDSTLAYQGYGRGLELDTVKCINSLASGGLVPDLTIWLDMDDKAALLRKGKLPPDRFESENNGF
HQRVRDGFGVIAAAEPNRFLKLDASLSQSELTKCIKQRVNALLKLPQ

Sequences:

>Translated_207_residues
MSLFITFEGGEGCGKSTQSKALYRYLKKLGLGCVLTHEPGGTRSGDKITRLLKWSEEENISPLAELFLFNASRSILIDNV
IKPALLDGNIVICDRYTDSTLAYQGYGRGLELDTVKCINSLASGGLVPDLTIWLDMDDKAALLRKGKLPPDRFESENNGF
HQRVRDGFGVIAAAEPNRFLKLDASLSQSELTKCIKQRVNALLKLPQ
>Mature_206_residues
SLFITFEGGEGCGKSTQSKALYRYLKKLGLGCVLTHEPGGTRSGDKITRLLKWSEEENISPLAELFLFNASRSILIDNVI
KPALLDGNIVICDRYTDSTLAYQGYGRGLELDTVKCINSLASGGLVPDLTIWLDMDDKAALLRKGKLPPDRFESENNGFH
QRVRDGFGVIAAAEPNRFLKLDASLSQSELTKCIKQRVNALLKLPQ

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=210, Percent_Identity=35.7142857142857, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_DEHE1 (Q3Z8D9)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181510.1
- HSSP:   P65248
- ProteinModelPortal:   Q3Z8D9
- SMR:   Q3Z8D9
- STRING:   Q3Z8D9
- GeneID:   3229893
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0778
- NMPDR:   fig|243164.3.peg.876
- TIGR:   DET0778
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- PhylomeDB:   Q3Z8D9
- ProtClustDB:   CLSK837283
- BioCyc:   DETH243164:DET_0778-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 22836; Mature: 22704

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFITFEGGEGCGKSTQSKALYRYLKKLGLGCVLTHEPGGTRSGDKITRLLKWSEEENI
CEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
SPLAELFLFNASRSILIDNVIKPALLDGNIVICDRYTDSTLAYQGYGRGLELDTVKCINS
CHHHHHHHHCCCCHHHHHHHCCCEEECCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHH
LASGGLVPDLTIWLDMDDKAALLRKGKLPPDRFESENNGFHQRVRDGFGVIAAAEPNRFL
HHCCCCCCCEEEEEECCCHHHHHHCCCCCHHHCCCCCCCHHHHHHCCCEEEEECCCCCEE
KLDASLSQSELTKCIKQRVNALLKLPQ
EECCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLFITFEGGEGCGKSTQSKALYRYLKKLGLGCVLTHEPGGTRSGDKITRLLKWSEEENI
EEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
SPLAELFLFNASRSILIDNVIKPALLDGNIVICDRYTDSTLAYQGYGRGLELDTVKCINS
CHHHHHHHHCCCCHHHHHHHCCCEEECCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHH
LASGGLVPDLTIWLDMDDKAALLRKGKLPPDRFESENNGFHQRVRDGFGVIAAAEPNRFL
HHCCCCCCCEEEEEECCCHHHHHHCCCCCHHHCCCCCCCHHHHHHCCCEEEEECCCCCEE
KLDASLSQSELTKCIKQRVNALLKLPQ
EECCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA