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The map label for this gene is thiE [H]

Identifier: 57234415

GI number: 57234415

Start: 718823

End: 719881

Strand: Direct

Name: thiE [H]

Synonym: DET0782

Alternate gene names: 57234415

Gene position: 718823-719881 (Clockwise)

Preceding gene: 57234416

Following gene: 57234414

Centisome position: 48.91

GC content: 49.58

Gene sequence:

>1059_bases
GTGGATAATCTTGATAAACTGTGGCGGATAGTTGATGTCAACCAGAACCGCTTAAGCGAAGGTTTACGGATACTGGAAGA
GATTGCCCGTTTGTACCTTGAAGATGAAACGCTGACTTCGCGTTTAAAAAACCTGCGTCACTCACTTACTTTACAGGATA
TCACCTCTAATTCCCGCCTTTTATTTTCCCGCCAGGCGGATACGGATATCGGCGCTCAGTTAGAAACAGCTGACCAGTCC
AAACCGGAAACCTTATTTTCGGTTGTAAGTGCCAATGCCAAGCGGGCAGAACAATCCCTGCGTGTTTTAGAAGAGTTTGC
CGGTTTATCTGAGACGGGTCTTGATGCCGCTCTCTACAGCCGCGGGCGTTTTGAACTTTATACCCTTGAAAAAGACCTGG
CGGCCAGGCTTTTGCGTAAACACCGCCGGGATATGATAACGGGGTTGTATGTGGCTATAGATGCAGATTATCTGGCCGGG
CGGGATATACCTGCAGTTACCAGAGAAGCCCTTGAAGGCGGTTGCCGCCTTATCCAGCTTAGGGCTAAAACGGCCTCTAC
CCGAAAGTTTCTGGCACTGGCCGTAAACTTGAAGGAAATTTGCCTTGAATACGGTGCGCTTTTTATTGTAAATGACCGCC
TGGATATTGCCCTAGCCTGCGGTGCGGACGGGCTTCATCTGGGGCAGACAGATATGCCGCTTTCGCAAGCCCGCCGCTTT
CTGCCGCCGGATAGCATTATAGGTATTTCTGCAGATACGCCCGAACAGGCTGTTTCAGCTCAAAACGAAGGGGCGGACTA
TGTGGCGGCCGGGGCGGTTTTTCCCACCCAAACTAAACCAGATGTCCTTTTCGGCGGTCTTTCAGGTTTAAAGGCCATTC
ACCAGGTGGTAAAAATACCTCTGGTGGCTATTGGCGGCATAAACAAGTCAAATTTTTATGAAGCCATGCAGGCAGGTGCG
GACAGCCTCTGTCTGATAAGTGCGGTCTTAGGTGCGCCTGATATTAAAAAGGCTACTTCAGAATTTATAACCCTTATGGA
GGCAGCAAAAATTGACTAG

Upstream 100 bases:

>100_bases
CAGTAGACCTTAGCCAGCCTGAACCTAAACTGGAACTTATTAAAAACGCACTGGGTGAAGAATAAAGACGAAAGATACGG
GTTTGTGTTAAAATCTTGGC

Downstream 100 bases:

>100_bases
TATCAGTCTCAAAATGGAACAGATATCTGACAGCATACGGGAAAATTTTAAAACCCGTGATGCCGCCCGTGAAAAATCCC
TGCCTGTCAGCCGGGAAGCT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MDNLDKLWRIVDVNQNRLSEGLRILEEIARLYLEDETLTSRLKNLRHSLTLQDITSNSRLLFSRQADTDIGAQLETADQS
KPETLFSVVSANAKRAEQSLRVLEEFAGLSETGLDAALYSRGRFELYTLEKDLAARLLRKHRRDMITGLYVAIDADYLAG
RDIPAVTREALEGGCRLIQLRAKTASTRKFLALAVNLKEICLEYGALFIVNDRLDIALACGADGLHLGQTDMPLSQARRF
LPPDSIIGISADTPEQAVSAQNEGADYVAAGAVFPTQTKPDVLFGGLSGLKAIHQVVKIPLVAIGGINKSNFYEAMQAGA
DSLCLISAVLGAPDIKKATSEFITLMEAAKID

Sequences:

>Translated_352_residues
MDNLDKLWRIVDVNQNRLSEGLRILEEIARLYLEDETLTSRLKNLRHSLTLQDITSNSRLLFSRQADTDIGAQLETADQS
KPETLFSVVSANAKRAEQSLRVLEEFAGLSETGLDAALYSRGRFELYTLEKDLAARLLRKHRRDMITGLYVAIDADYLAG
RDIPAVTREALEGGCRLIQLRAKTASTRKFLALAVNLKEICLEYGALFIVNDRLDIALACGADGLHLGQTDMPLSQARRF
LPPDSIIGISADTPEQAVSAQNEGADYVAAGAVFPTQTKPDVLFGGLSGLKAIHQVVKIPLVAIGGINKSNFYEAMQAGA
DSLCLISAVLGAPDIKKATSEFITLMEAAKID
>Mature_352_residues
MDNLDKLWRIVDVNQNRLSEGLRILEEIARLYLEDETLTSRLKNLRHSLTLQDITSNSRLLFSRQADTDIGAQLETADQS
KPETLFSVVSANAKRAEQSLRVLEEFAGLSETGLDAALYSRGRFELYTLEKDLAARLLRKHRRDMITGLYVAIDADYLAG
RDIPAVTREALEGGCRLIQLRAKTASTRKFLALAVNLKEICLEYGALFIVNDRLDIALACGADGLHLGQTDMPLSQARRF
LPPDSIIGISADTPEQAVSAQNEGADYVAAGAVFPTQTKPDVLFGGLSGLKAIHQVVKIPLVAIGGINKSNFYEAMQAGA
DSLCLISAVLGAPDIKKATSEFITLMEAAKID

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=201, Percent_Identity=27.8606965174129, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325042, Length=222, Percent_Identity=31.0810810810811, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38434; Mature: 38434

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLDKLWRIVDVNQNRLSEGLRILEEIARLYLEDETLTSRLKNLRHSLTLQDITSNSRL
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHCCCCCCEE
LFSRQADTDIGAQLETADQSKPETLFSVVSANAKRAEQSLRVLEEFAGLSETGLDAALYS
EEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
RGRFELYTLEKDLAARLLRKHRRDMITGLYVAIDADYLAGRDIPAVTREALEGGCRLIQL
CCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEECHHHCCCCCHHHHHHHHHCCCEEEEE
RAKTASTRKFLALAVNLKEICLEYGALFIVNDRLDIALACGADGLHLGQTDMPLSQARRF
ECHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHC
LPPDSIIGISADTPEQAVSAQNEGADYVAAGAVFPTQTKPDVLFGGLSGLKAIHQVVKIP
CCCCCEEECCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
LVAIGGINKSNFYEAMQAGADSLCLISAVLGAPDIKKATSEFITLMEAAKID
HHEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDNLDKLWRIVDVNQNRLSEGLRILEEIARLYLEDETLTSRLKNLRHSLTLQDITSNSRL
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHCCCCCCEE
LFSRQADTDIGAQLETADQSKPETLFSVVSANAKRAEQSLRVLEEFAGLSETGLDAALYS
EEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHC
RGRFELYTLEKDLAARLLRKHRRDMITGLYVAIDADYLAGRDIPAVTREALEGGCRLIQL
CCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEECHHHCCCCCHHHHHHHHHCCCEEEEE
RAKTASTRKFLALAVNLKEICLEYGALFIVNDRLDIALACGADGLHLGQTDMPLSQARRF
ECHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHC
LPPDSIIGISADTPEQAVSAQNEGADYVAAGAVFPTQTKPDVLFGGLSGLKAIHQVVKIP
CCCCCEEECCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
LVAIGGINKSNFYEAMQAGADSLCLISAVLGAPDIKKATSEFITLMEAAKID
HHEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]