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The map label for this gene is sbcD [H]

Identifier: 57234402

GI number: 57234402

Start: 725205

End: 726452

Strand: Direct

Name: sbcD [H]

Synonym: DET0788

Alternate gene names: 57234402

Gene position: 725205-726452 (Clockwise)

Preceding gene: 57234403

Following gene: 57234401

Centisome position: 49.34

GC content: 48.64

Gene sequence:

>1248_bases
TTGAAAATCCTCCACTTTGCAGACCTTCACATAGGGGTGGAGAATTACGGCCGTTTTGACAGTGCAACCGGGCTTTCCTC
CCGGCTGGCTGATTTCTTGGCTGCTTTTGACCGTTTGGTGGCTTATGCCATTGAGAACAAAGTGGATTTGGTGGTTTTCA
GCGGTGATGCTTACAAAAGCCGTGACCCTTCCCAAACTCAGCAGCGGGAGTTCGCCCGTCGGATAAGTACCCTGGCAAAT
AACGGCATACAGGTATTCCTGCTAGTAGGAAACCATGACCTGCCGAATGCCACCGGCAGGGCTACCACAACTGAAATATT
TGACACACTTAATATTGCCAATGTGCATGTTTCCGCCAAGGCGGAAGTGCGGCTGATAAATACCCGCAGCGGTCTTATTC
AGGTCGCCTCCCTGCCGTGGTTACGCCGTTCCTCTGTTCTTTCGGTCTCAAACCAGAAAGAAGAAAAAAGCCTGAGCATT
GAGGAACTGAACCAGAGGGTGGAACATTATCTGGCCGGTATTATAGAAAATCTGGCAGGCCAGCTTGATAAAGATATCCC
CGCCATCCTTTCCGCCCATCTTTCGGTCAATACCGCCAAACTGGGCTCCGAGCGGAATATCTCAATAGGTCACGAGCCTA
CAGTTATGTTAAGTAATATTGCCAACCCTGCTTTTGATTATATTGCTCTGGGGCATATCCATAAGCAGCAGGTATTGTCT
TCTTCCCCTCCGGTAGTGTATCCCGGCAGTCTGGAAAGGCTGGATTTCGGTGAGGAAAAAGACGATAAAGGTTTTTATCT
GGTAGAGATAGACTCCCTCAGCCGTAAAACTGAATTTGAGTTTATAAAACTGGAAGGCCGCCGTTTTTTGACTATAGAGC
ATACTGTAACGGCAGACAGCCTTGACCCTACTCAGGAAGTAATAAATATATTACAATCACGCCAGCCGGAAATAAAAGAT
GCTATAGTCCGCCTGAAACTGGAGCTGCCGGCAGAGATTGGCGGGCAGCTGCGGGATAATGATATACGGGAAGCTTTGCG
TGAGGCCCATAACTTCACTGTTTCCAAAAATTTCACCCGCTCAAGCCGCCAAAGAGAGGGCGGTTTCAAAGCGGAGGGGC
TTGACCCTGTTCAGGCTTTGGAACATTATTTGGATGCCAGGCAGACTTCGGCACAGGAGAAAAAGACTCTGCTGGAATAT
GGGCGCAGGCTGTATACTTCACTGGATAGCAAGCAGACAAAAGTTTAA

Upstream 100 bases:

>100_bases
GATAGCATGGATCAAATACGGGTAAAAGTATCCGAACTTAGCTGCATTCTGGCTGCCAAGCTTTCGGGTGAGCCGTTTAA
CAGCTGATTTGGAGGCGAAA

Downstream 100 bases:

>100_bases
TACCCATTTAAAGGAATTTTAATGTCATATAATGCATCTTCAGGTACTCCGTTGGTGAAAGTTTTAGTGGAAGCCAGGCT
TGGTTCACGCCGCAAAATGG

Product: nuclease SbcCD, D subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKILHFADLHIGVENYGRFDSATGLSSRLADFLAAFDRLVAYAIENKVDLVVFSGDAYKSRDPSQTQQREFARRISTLAN
NGIQVFLLVGNHDLPNATGRATTTEIFDTLNIANVHVSAKAEVRLINTRSGLIQVASLPWLRRSSVLSVSNQKEEKSLSI
EELNQRVEHYLAGIIENLAGQLDKDIPAILSAHLSVNTAKLGSERNISIGHEPTVMLSNIANPAFDYIALGHIHKQQVLS
SSPPVVYPGSLERLDFGEEKDDKGFYLVEIDSLSRKTEFEFIKLEGRRFLTIEHTVTADSLDPTQEVINILQSRQPEIKD
AIVRLKLELPAEIGGQLRDNDIREALREAHNFTVSKNFTRSSRQREGGFKAEGLDPVQALEHYLDARQTSAQEKKTLLEY
GRRLYTSLDSKQTKV

Sequences:

>Translated_415_residues
MKILHFADLHIGVENYGRFDSATGLSSRLADFLAAFDRLVAYAIENKVDLVVFSGDAYKSRDPSQTQQREFARRISTLAN
NGIQVFLLVGNHDLPNATGRATTTEIFDTLNIANVHVSAKAEVRLINTRSGLIQVASLPWLRRSSVLSVSNQKEEKSLSI
EELNQRVEHYLAGIIENLAGQLDKDIPAILSAHLSVNTAKLGSERNISIGHEPTVMLSNIANPAFDYIALGHIHKQQVLS
SSPPVVYPGSLERLDFGEEKDDKGFYLVEIDSLSRKTEFEFIKLEGRRFLTIEHTVTADSLDPTQEVINILQSRQPEIKD
AIVRLKLELPAEIGGQLRDNDIREALREAHNFTVSKNFTRSSRQREGGFKAEGLDPVQALEHYLDARQTSAQEKKTLLEY
GRRLYTSLDSKQTKV
>Mature_415_residues
MKILHFADLHIGVENYGRFDSATGLSSRLADFLAAFDRLVAYAIENKVDLVVFSGDAYKSRDPSQTQQREFARRISTLAN
NGIQVFLLVGNHDLPNATGRATTTEIFDTLNIANVHVSAKAEVRLINTRSGLIQVASLPWLRRSSVLSVSNQKEEKSLSI
EELNQRVEHYLAGIIENLAGQLDKDIPAILSAHLSVNTAKLGSERNISIGHEPTVMLSNIANPAFDYIALGHIHKQQVLS
SSPPVVYPGSLERLDFGEEKDDKGFYLVEIDSLSRKTEFEFIKLEGRRFLTIEHTVTADSLDPTQEVINILQSRQPEIKD
AIVRLKLELPAEIGGQLRDNDIREALREAHNFTVSKNFTRSSRQREGGFKAEGLDPVQALEHYLDARQTSAQEKKTLLEY
GRRLYTSLDSKQTKV

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=283, Percent_Identity=29.3286219081272, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46448; Mature: 46448

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILHFADLHIGVENYGRFDSATGLSSRLADFLAAFDRLVAYAIENKVDLVVFSGDAYKS
CCEEEEEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
RDPSQTQQREFARRISTLANNGIQVFLLVGNHDLPNATGRATTTEIFDTLNIANVHVSAK
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEEEECC
AEVRLINTRSGLIQVASLPWLRRSSVLSVSNQKEEKSLSIEELNQRVEHYLAGIIENLAG
EEEEEEECCCCEEEECCCCCHHCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QLDKDIPAILSAHLSVNTAKLGSERNISIGHEPTVMLSNIANPAFDYIALGHIHKQQVLS
HHCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHC
SSPPVVYPGSLERLDFGEEKDDKGFYLVEIDSLSRKTEFEFIKLEGRRFLTIEHTVTADS
CCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCEEEEEEEEEECCC
LDPTQEVINILQSRQPEIKDAIVRLKLELPAEIGGQLRDNDIREALREAHNFTVSKNFTR
CCHHHHHHHHHHCCCCHHHHHHEEEEEECCHHHCCCCCCHHHHHHHHHHHCCEEECCCHH
SSRQREGGFKAEGLDPVQALEHYLDARQTSAQEKKTLLEYGRRLYTSLDSKQTKV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKILHFADLHIGVENYGRFDSATGLSSRLADFLAAFDRLVAYAIENKVDLVVFSGDAYKS
CCEEEEEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
RDPSQTQQREFARRISTLANNGIQVFLLVGNHDLPNATGRATTTEIFDTLNIANVHVSAK
CCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEEEECC
AEVRLINTRSGLIQVASLPWLRRSSVLSVSNQKEEKSLSIEELNQRVEHYLAGIIENLAG
EEEEEEECCCCEEEECCCCCHHCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QLDKDIPAILSAHLSVNTAKLGSERNISIGHEPTVMLSNIANPAFDYIALGHIHKQQVLS
HHCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHC
SSPPVVYPGSLERLDFGEEKDDKGFYLVEIDSLSRKTEFEFIKLEGRRFLTIEHTVTADS
CCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCEEEEEEEEEECCC
LDPTQEVINILQSRQPEIKDAIVRLKLELPAEIGGQLRDNDIREALREAHNFTVSKNFTR
CCHHHHHHHHHHCCCCHHHHHHEEEEEECCHHHCCCCCCHHHHHHHHHHHCCEEECCCHH
SSRQREGGFKAEGLDPVQALEHYLDARQTSAQEKKTLLEYGRRLYTSLDSKQTKV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]