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The map label for this gene is ndhF [H]

Identifier: 57234291

GI number: 57234291

Start: 850542

End: 852506

Strand: Direct

Name: ndhF [H]

Synonym: DET0931

Alternate gene names: 57234291

Gene position: 850542-852506 (Clockwise)

Preceding gene: 57234300

Following gene: 57234290

Centisome position: 57.87

GC content: 51.09

Gene sequence:

>1965_bases
ATGACCCAAACCGGACTCTGGCTCATAATGCTGCTCCCCCTTATCTGCGGGGCTTTGATAGCCCTGGGGTACTCCTTTTT
CTGCAAAAAACCGGCTTTAAGCGGATACCTAGGCATAGCCGGTGTAGGTGGTTCGCTGCTACTTTCTATCTGGGGGCTGA
CAGGCCTGCTGGGAAAGGAAGGGGCTTCTCACTATGCCTCCGGCTTCAGCTGGTTTTCAATTGGTAATTCGGTTGATATC
AGCCTTAACCTAAACTTTGACCCGCTGGCCGCCATAATGTGCTTTGTAATCCTTTTTGTCAGCCTGATGGTACACATATA
TTCACAGGGCTATATGCACGGTGATAAAGGGTATCCCCGTTACTATGCCTTTTTGTCTTTCTTTACCGCCTCTATGCTGG
GGCTGGTGCTCTCTGACAACCTGCTTTTTACCTTCTTCTTTTGGGAGTTGGTGGGTCTGGCATCATATCTTCTTATCGGC
TTCTGGTTTACCCGCCCGGCCGCCGCCAATGCCGCTAAAAAGGCGTTCATTGTAACCCGTATAGGTGATGTGGGCTTTTT
AGCCGCTATACTCATTTTGTTTGCGAATACCGGCACGCTGGATATAAACAGCCTGAACGGCATGGCCGAAATGGGGCTTA
TAGGTGCCGGCACAGTAACTGTGGCCGCTCTGGGCATATTTGCCGGTGCGGTGGGTAAATCAGCCCAGTTCCCCCTGCAT
GTATGGCTGCCTGATGCTATGGAAGGCCCTACCCCCGTTTCCGCCCTTATCCATGCCGCCACCATGGTTGCTGCCGGTGT
ATTTCTGGTTGCCCGCACCTACCCTATATTTGAAAGCTCGGCAACAGCCATGCTTTGGGTATCTATAATCGGTGCGGTTA
CCGCTATATTTGCCGCTACCATGGCGCTGGTTATGAACGATATGAAGCGTATACTGGCCTATTCCACAGTCAGCCAGCTG
GGTTATATGATGCTGGGGCTGGGTACAGGCGGCATTGCTATCGGTATATTCCACCTGTTTAATCATGCTTTTTTCAAGAG
TTTGCTCTTTTTAGGTTCGGGCAGCGTAAATCACGCTACCGGCACATTTGATATCCGCGAAATGGGCGGCCTGTCCAAAC
CCATGCCTGTTACCAGCAAGACTTTTCTTATTGCCTCACTCAGTCTGGCAGGTATCTGGCCGCTTTCAGGCTTTTTCAGC
AAGGACGAGATACTTGCCGGGGCTATGGACGGGCAATTTATTCTGTTCATTCTGGCACTCATCACCGTATTCCTGACCGC
CTTTTATATGTTCCGCCTGTACTTCGTGGCTTTCGGCGGCACTTACCGCGGCAAGGGGCATCCCCATGAATCCCCCAAGG
TCATGTCATGGCCGCTTCTGATACTGGTTGTGCCTTCGGTAATCAGCGGTTTGCTGAATGCCGGCGGCAGTTTCAGCGGC
TTTTTGGGCGAAGAGGTGCATACCGGCTTTTTTGAGGGATTATTCGGCATTTTGCTGCACCCGCTGGCCCTGGTTTCACT
GGCCGTAGCCGGCGGCGGTATATATCTGGCTTTCCTGATGTATAAAAAGAAGAGCCTTTCACCCGCTGTATTTTCAGAGC
GTTTTAAATGGCTTTATATGATATTTTCCAAGAAATACTGGATGGATGAGCTGTACGAAGGTGTTATATCCCGCACGCTG
CTTATGAAGGGACTGTTTGCTTTTGCGGCTTTCTTTGACAAAAAGGTGGTAGACGGGGGCTTAAACGGCTTCTTCATTCA
AAAAGTATTATTTTCCCGTCTTTTCAGCCGCTTCCGCACCGCTGATGAACGCATAGTGGACGGGGCGGTAAACGGGGTTG
CCGCTATAACCGTAGAGAGCGGCAAGGTGGGACGCAAAGCCCAGACCGGGCAGCTCCAATCATACGGAATTTATCTTGCC
GCCGGGGTGGTTGTACTGGTGATGGCGGCTCTTATTATCTGGTAA

Upstream 100 bases:

>100_bases
ACTATTGGTCTGGCCATAATAATATCCATTTACAAAAACCGGGATACTATTGATGCCACCAAGATAGATTTGATGAAGTG
GTAAAGAGGAAGAATAAGCG

Downstream 100 bases:

>100_bases
GGGGGGAGTATGAGCATACCATACTTAACACTAATAACTTTCCTGCCCGCAGCGGGAGCTATCTTGATGGCTCTGTGGCC
GCGTTTGTCCGGGCGGGCAA

Product: proton-translocating NADH-quinone oxidoreductase, L subunit

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MTQTGLWLIMLLPLICGALIALGYSFFCKKPALSGYLGIAGVGGSLLLSIWGLTGLLGKEGASHYASGFSWFSIGNSVDI
SLNLNFDPLAAIMCFVILFVSLMVHIYSQGYMHGDKGYPRYYAFLSFFTASMLGLVLSDNLLFTFFFWELVGLASYLLIG
FWFTRPAAANAAKKAFIVTRIGDVGFLAAILILFANTGTLDINSLNGMAEMGLIGAGTVTVAALGIFAGAVGKSAQFPLH
VWLPDAMEGPTPVSALIHAATMVAAGVFLVARTYPIFESSATAMLWVSIIGAVTAIFAATMALVMNDMKRILAYSTVSQL
GYMMLGLGTGGIAIGIFHLFNHAFFKSLLFLGSGSVNHATGTFDIREMGGLSKPMPVTSKTFLIASLSLAGIWPLSGFFS
KDEILAGAMDGQFILFILALITVFLTAFYMFRLYFVAFGGTYRGKGHPHESPKVMSWPLLILVVPSVISGLLNAGGSFSG
FLGEEVHTGFFEGLFGILLHPLALVSLAVAGGGIYLAFLMYKKKSLSPAVFSERFKWLYMIFSKKYWMDELYEGVISRTL
LMKGLFAFAAFFDKKVVDGGLNGFFIQKVLFSRLFSRFRTADERIVDGAVNGVAAITVESGKVGRKAQTGQLQSYGIYLA
AGVVVLVMAALIIW

Sequences:

>Translated_654_residues
MTQTGLWLIMLLPLICGALIALGYSFFCKKPALSGYLGIAGVGGSLLLSIWGLTGLLGKEGASHYASGFSWFSIGNSVDI
SLNLNFDPLAAIMCFVILFVSLMVHIYSQGYMHGDKGYPRYYAFLSFFTASMLGLVLSDNLLFTFFFWELVGLASYLLIG
FWFTRPAAANAAKKAFIVTRIGDVGFLAAILILFANTGTLDINSLNGMAEMGLIGAGTVTVAALGIFAGAVGKSAQFPLH
VWLPDAMEGPTPVSALIHAATMVAAGVFLVARTYPIFESSATAMLWVSIIGAVTAIFAATMALVMNDMKRILAYSTVSQL
GYMMLGLGTGGIAIGIFHLFNHAFFKSLLFLGSGSVNHATGTFDIREMGGLSKPMPVTSKTFLIASLSLAGIWPLSGFFS
KDEILAGAMDGQFILFILALITVFLTAFYMFRLYFVAFGGTYRGKGHPHESPKVMSWPLLILVVPSVISGLLNAGGSFSG
FLGEEVHTGFFEGLFGILLHPLALVSLAVAGGGIYLAFLMYKKKSLSPAVFSERFKWLYMIFSKKYWMDELYEGVISRTL
LMKGLFAFAAFFDKKVVDGGLNGFFIQKVLFSRLFSRFRTADERIVDGAVNGVAAITVESGKVGRKAQTGQLQSYGIYLA
AGVVVLVMAALIIW
>Mature_653_residues
TQTGLWLIMLLPLICGALIALGYSFFCKKPALSGYLGIAGVGGSLLLSIWGLTGLLGKEGASHYASGFSWFSIGNSVDIS
LNLNFDPLAAIMCFVILFVSLMVHIYSQGYMHGDKGYPRYYAFLSFFTASMLGLVLSDNLLFTFFFWELVGLASYLLIGF
WFTRPAAANAAKKAFIVTRIGDVGFLAAILILFANTGTLDINSLNGMAEMGLIGAGTVTVAALGIFAGAVGKSAQFPLHV
WLPDAMEGPTPVSALIHAATMVAAGVFLVARTYPIFESSATAMLWVSIIGAVTAIFAATMALVMNDMKRILAYSTVSQLG
YMMLGLGTGGIAIGIFHLFNHAFFKSLLFLGSGSVNHATGTFDIREMGGLSKPMPVTSKTFLIASLSLAGIWPLSGFFSK
DEILAGAMDGQFILFILALITVFLTAFYMFRLYFVAFGGTYRGKGHPHESPKVMSWPLLILVVPSVISGLLNAGGSFSGF
LGEEVHTGFFEGLFGILLHPLALVSLAVAGGGIYLAFLMYKKKSLSPAVFSERFKWLYMIFSKKYWMDELYEGVISRTLL
MKGLFAFAAFFDKKVVDGGLNGFFIQKVLFSRLFSRFRTADERIVDGAVNGVAAITVESGKVGRKAQTGQLQSYGIYLAA
GVVVLVMAALIIW

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=380, Percent_Identity=40, Blast_Score=248, Evalue=1e-65,
Organism=Escherichia coli, GI1788614, Length=474, Percent_Identity=43.4599156118143, Blast_Score=329, Evalue=4e-91,
Organism=Escherichia coli, GI1788829, Length=422, Percent_Identity=36.2559241706161, Blast_Score=208, Evalue=1e-54,
Organism=Escherichia coli, GI1788827, Length=426, Percent_Identity=29.81220657277, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788831, Length=409, Percent_Identity=29.8288508557457, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI2367154, Length=491, Percent_Identity=27.9022403258656, Blast_Score=119, Evalue=9e-28,
Organism=Escherichia coli, GI1788613, Length=467, Percent_Identity=25.0535331905782, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI145693160, Length=355, Percent_Identity=27.3239436619718, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 70416; Mature: 70285

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTGLWLIMLLPLICGALIALGYSFFCKKPALSGYLGIAGVGGSLLLSIWGLTGLLGKE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GASHYASGFSWFSIGNSVDISLNLNFDPLAAIMCFVILFVSLMVHIYSQGYMHGDKGYPR
CCHHHHCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHH
YYAFLSFFTASMLGLVLSDNLLFTFFFWELVGLASYLLIGFWFTRPAAANAAKKAFIVTR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHEEEEEE
IGDVGFLAAILILFANTGTLDINSLNGMAEMGLIGAGTVTVAALGIFAGAVGKSAQFPLH
CCHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
VWLPDAMEGPTPVSALIHAATMVAAGVFLVARTYPIFESSATAMLWVSIIGAVTAIFAAT
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MALVMNDMKRILAYSTVSQLGYMMLGLGTGGIAIGIFHLFNHAFFKSLLFLGSGSVNHAT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GTFDIREMGGLSKPMPVTSKTFLIASLSLAGIWPLSGFFSKDEILAGAMDGQFILFILAL
CCEEHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHH
ITVFLTAFYMFRLYFVAFGGTYRGKGHPHESPKVMSWPLLILVVPSVISGLLNAGGSFSG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
FLGEEVHTGFFEGLFGILLHPLALVSLAVAGGGIYLAFLMYKKKSLSPAVFSERFKWLYM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IFSKKYWMDELYEGVISRTLLMKGLFAFAAFFDKKVVDGGLNGFFIQKVLFSRLFSRFRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ADERIVDGAVNGVAAITVESGKVGRKAQTGQLQSYGIYLAAGVVVLVMAALIIW
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQTGLWLIMLLPLICGALIALGYSFFCKKPALSGYLGIAGVGGSLLLSIWGLTGLLGKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GASHYASGFSWFSIGNSVDISLNLNFDPLAAIMCFVILFVSLMVHIYSQGYMHGDKGYPR
CCHHHHCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHH
YYAFLSFFTASMLGLVLSDNLLFTFFFWELVGLASYLLIGFWFTRPAAANAAKKAFIVTR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHEEEEEE
IGDVGFLAAILILFANTGTLDINSLNGMAEMGLIGAGTVTVAALGIFAGAVGKSAQFPLH
CCHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
VWLPDAMEGPTPVSALIHAATMVAAGVFLVARTYPIFESSATAMLWVSIIGAVTAIFAAT
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
MALVMNDMKRILAYSTVSQLGYMMLGLGTGGIAIGIFHLFNHAFFKSLLFLGSGSVNHAT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GTFDIREMGGLSKPMPVTSKTFLIASLSLAGIWPLSGFFSKDEILAGAMDGQFILFILAL
CCEEHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHH
ITVFLTAFYMFRLYFVAFGGTYRGKGHPHESPKVMSWPLLILVVPSVISGLLNAGGSFSG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
FLGEEVHTGFFEGLFGILLHPLALVSLAVAGGGIYLAFLMYKKKSLSPAVFSERFKWLYM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IFSKKYWMDELYEGVISRTLLMKGLFAFAAFFDKKVVDGGLNGFFIQKVLFSRLFSRFRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ADERIVDGAVNGVAAITVESGKVGRKAQTGQLQSYGIYLAAGVVVLVMAALIIW
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]