Click here to switch to the map view.

The map label for this gene is nuoN [H]

Identifier: 57234289

GI number: 57234289

Start: 854028

End: 855473

Strand: Direct

Name: nuoN [H]

Synonym: DET0933

Alternate gene names: 57234289

Gene position: 854028-855473 (Clockwise)

Preceding gene: 57234290

Following gene: 57234288

Centisome position: 58.11

GC content: 48.76

Gene sequence:

>1446_bases
ATGCCTGAAATAATAATACTGATAACAGCCATTTTGGTTATAATAACTGACCTCTTTCTGACTAAAAGCAAGAGGTATCT
GGCCTATCTGTCTTTGCTGGGACTGGCAGCAGCGGCTGTAGCCACAGCTCTAAACTGGAATAACCCACCCGAAATGGCTT
TTGGCGGTATGCTGGCACTGGACAGCTACTCCAGTTTCTTCCGTATTCTTTTTATCTGCCTTTCAGGCCTGGTTATAATG
GCTTCTACTGATTATGTAACTAAGTTTAAACGTTTTCAGGGTGAATACTACGCTCTGGTACTGTTTGCCCTGCTGGGCAT
GATAATGATGGCCTCCACGGCCAACCTTATTACCATGTATCTTTCACTAGAGCTGACCGGACTGTCTTTTTACGTACTGG
TGGGTTTTTTAAAAGACCAGAATTCCACCGAATCAGCTCTCAAATACCTGCTTTTGGGCGGGGTGGCTTCGGCTATGCTG
GTGTTTGGCCTGGTGATTATTTACGGTTTCAGCGGCGAAACTAATCTGGGCGGTATTGTAAACTATATACAAACCCTGCC
CTCAGGCACAGATATAACCACTCATGCAGGGCTGCTGCTGGGTATAATACTGACCATTACAGGTCTGGGCTTTAAAGTAG
CCGCCGTGCCATTCCAATTTTGGGTGCCGGACGTTTATCAGGGCTCTCCCACCCCGATAACATTATACCTTTCCATAGCC
AGCAAAGCGGCCGGGTTTGCCCTTTTCCTACGTTTGTTTTACACCGTTTTTACCGACCCGCTGGCACTCAGCCAGGAATG
GGCACTGATTATTGCCATTCTGGCCGCTGTGGGTATGACACTGGGAAATGTACTGGCCATACCCCAGAAAAACATCAAAA
GAATGCTGGGTTACTCCAGCATAGCCCATGCCGGTTATATACTGGTGGCTCTGGCAGCGGTGGGAAACGCCCCGGAACTG
GCAGACGGGCGGATAAGCCTGCTCTTTTATCTGGTGGCTTTTGCCGTATCAGACCTGGCCGCCTTTATCTCTATCATAGC
CATCAGCCGTTCTACCGGCAGTGATGAAATATCCTCTTACGAAGGCTTAGCCAAGACCAACCCGGTATATGCTTCTGCCC
TGACACTGGCGCTGTTGTCTCTCACCGGTTTTCCGCCTCTGGCAGGTTTCCTTGCCAAGTATTATATTTTCAGCGCATCT
GTCCAGGCGGATATGCTCTGGCTGATGATTATCGCCGCCGTAAACACCGTGATTTCGGCCGTTTTTTACTTCAACGTAAT
ACGGGTAATGTGGCTAAGACCTGCCCGCCAGGATGTACGGGTACTGGCTTCCTGGCCGCTTAAACTGGCACTTGGTATTT
CGGGACTGGCTGTGTTAATATTCGGCATTGTACCTGAAACACTCTTAAACCTTATTGAAAAGGCCGCCGAGCTAATAATC
CATTAG

Upstream 100 bases:

>100_bases
CGGGGTTGGCCTTTATCCCCAGATTATAGTAGATGTGATTCAAACCGGAGTGGCCCCGCTGGCGGCCGTTTTCGGCGGAT
AGAGGCAGATGGATTTGTTT

Downstream 100 bases:

>100_bases
GACATTTGCCGTGCCGATTTATGAATATGTAGACACCCATTCCCATCTGGATATGCCTGAATTTGACGCTGACCGTCAGG
AAATGCTAAAGCGGGCTTTT

Product: proton-translocating NADH-quinone oxidoreductase, N subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MPEIIILITAILVIITDLFLTKSKRYLAYLSLLGLAAAAVATALNWNNPPEMAFGGMLALDSYSSFFRILFICLSGLVIM
ASTDYVTKFKRFQGEYYALVLFALLGMIMMASTANLITMYLSLELTGLSFYVLVGFLKDQNSTESALKYLLLGGVASAML
VFGLVIIYGFSGETNLGGIVNYIQTLPSGTDITTHAGLLLGIILTITGLGFKVAAVPFQFWVPDVYQGSPTPITLYLSIA
SKAAGFALFLRLFYTVFTDPLALSQEWALIIAILAAVGMTLGNVLAIPQKNIKRMLGYSSIAHAGYILVALAAVGNAPEL
ADGRISLLFYLVAFAVSDLAAFISIIAISRSTGSDEISSYEGLAKTNPVYASALTLALLSLTGFPPLAGFLAKYYIFSAS
VQADMLWLMIIAAVNTVISAVFYFNVIRVMWLRPARQDVRVLASWPLKLALGISGLAVLIFGIVPETLLNLIEKAAELII
H

Sequences:

>Translated_481_residues
MPEIIILITAILVIITDLFLTKSKRYLAYLSLLGLAAAAVATALNWNNPPEMAFGGMLALDSYSSFFRILFICLSGLVIM
ASTDYVTKFKRFQGEYYALVLFALLGMIMMASTANLITMYLSLELTGLSFYVLVGFLKDQNSTESALKYLLLGGVASAML
VFGLVIIYGFSGETNLGGIVNYIQTLPSGTDITTHAGLLLGIILTITGLGFKVAAVPFQFWVPDVYQGSPTPITLYLSIA
SKAAGFALFLRLFYTVFTDPLALSQEWALIIAILAAVGMTLGNVLAIPQKNIKRMLGYSSIAHAGYILVALAAVGNAPEL
ADGRISLLFYLVAFAVSDLAAFISIIAISRSTGSDEISSYEGLAKTNPVYASALTLALLSLTGFPPLAGFLAKYYIFSAS
VQADMLWLMIIAAVNTVISAVFYFNVIRVMWLRPARQDVRVLASWPLKLALGISGLAVLIFGIVPETLLNLIEKAAELII
H
>Mature_480_residues
PEIIILITAILVIITDLFLTKSKRYLAYLSLLGLAAAAVATALNWNNPPEMAFGGMLALDSYSSFFRILFICLSGLVIMA
STDYVTKFKRFQGEYYALVLFALLGMIMMASTANLITMYLSLELTGLSFYVLVGFLKDQNSTESALKYLLLGGVASAMLV
FGLVIIYGFSGETNLGGIVNYIQTLPSGTDITTHAGLLLGIILTITGLGFKVAAVPFQFWVPDVYQGSPTPITLYLSIAS
KAAGFALFLRLFYTVFTDPLALSQEWALIIAILAAVGMTLGNVLAIPQKNIKRMLGYSSIAHAGYILVALAAVGNAPELA
DGRISLLFYLVAFAVSDLAAFISIIAISRSTGSDEISSYEGLAKTNPVYASALTLALLSLTGFPPLAGFLAKYYIFSASV
QADMLWLMIIAAVNTVISAVFYFNVIRVMWLRPARQDVRVLASWPLKLALGISGLAVLIFGIVPETLLNLIEKAAELIIH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=500, Percent_Identity=32.4, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788829, Length=402, Percent_Identity=26.6169154228856, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788831, Length=316, Percent_Identity=23.1012658227848, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788614, Length=357, Percent_Identity=24.9299719887955, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788827, Length=362, Percent_Identity=20.9944751381215, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367154, Length=299, Percent_Identity=25.0836120401338, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 51815; Mature: 51684

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIIILITAILVIITDLFLTKSKRYLAYLSLLGLAAAAVATALNWNNPPEMAFGGMLAL
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHH
DSYSSFFRILFICLSGLVIMASTDYVTKFKRFQGEYYALVLFALLGMIMMASTANLITMY
HHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLELTGLSFYVLVGFLKDQNSTESALKYLLLGGVASAMLVFGLVIIYGFSGETNLGGIV
HHHEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
NYIQTLPSGTDITTHAGLLLGIILTITGLGFKVAAVPFQFWVPDVYQGSPTPITLYLSIA
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEEH
SKAAGFALFLRLFYTVFTDPLALSQEWALIIAILAAVGMTLGNVLAIPQKNIKRMLGYSS
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCHHH
IAHAGYILVALAAVGNAPELADGRISLLFYLVAFAVSDLAAFISIIAISRSTGSDEISSY
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EGLAKTNPVYASALTLALLSLTGFPPLAGFLAKYYIFSASVQADMLWLMIIAAVNTVISA
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VFYFNVIRVMWLRPARQDVRVLASWPLKLALGISGLAVLIFGIVPETLLNLIEKAAELII
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
H
C
>Mature Secondary Structure 
PEIIILITAILVIITDLFLTKSKRYLAYLSLLGLAAAAVATALNWNNPPEMAFGGMLAL
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHH
DSYSSFFRILFICLSGLVIMASTDYVTKFKRFQGEYYALVLFALLGMIMMASTANLITMY
HHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLELTGLSFYVLVGFLKDQNSTESALKYLLLGGVASAMLVFGLVIIYGFSGETNLGGIV
HHHEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
NYIQTLPSGTDITTHAGLLLGIILTITGLGFKVAAVPFQFWVPDVYQGSPTPITLYLSIA
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCEEEEEEEH
SKAAGFALFLRLFYTVFTDPLALSQEWALIIAILAAVGMTLGNVLAIPQKNIKRMLGYSS
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCHHH
IAHAGYILVALAAVGNAPELADGRISLLFYLVAFAVSDLAAFISIIAISRSTGSDEISSY
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EGLAKTNPVYASALTLALLSLTGFPPLAGFLAKYYIFSASVQADMLWLMIIAAVNTVISA
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VFYFNVIRVMWLRPARQDVRVLASWPLKLALGISGLAVLIFGIVPETLLNLIEKAAELII
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA