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The map label for this gene is trpB2 [H]

Identifier: 57234212

GI number: 57234212

Start: 938654

End: 940018

Strand: Direct

Name: trpB2 [H]

Synonym: DET1035

Alternate gene names: 57234212

Gene position: 938654-940018 (Clockwise)

Preceding gene: 57234213

Following gene: 57234204

Centisome position: 63.87

GC content: 53.41

Gene sequence:

>1365_bases
ATGGCTCAAACCAAGTTTCTACTGCAAGAAAAGGATATGCCCCAGGTCTGGTACAATATCCAGCCTGATTTACCCGTACC
GCTGCCCCCGGTTCTGCACCCGGGTACCGGCCAGCCGGTAACTCCGGCTGATATGGAAGCCATATTCCCCATGAGCCTTA
TCATGCAGGAGTTCAGCCCCGAAAGGCATATACCCATCCCCGAAGAACTGCTGGAAATATATAAAATCTGGCGGCCTACC
CCCCTTTACCGCGCCCACAGGCTGGAAAAGGCCTTGCAGACCCCGGCCAAAATCTTTTACAAACTGGAAGATGTCAGCCC
GGCCGGTTCTCACAAACCCAATACTGCTCTGGCTCAGGCCTATTACAATAAAAAAGCCGGCATAAAGAGGATTTCCACCG
AAACCGGCGCCGGACAGTGGGGCTCTTCGCTGGCCATGGCCTGCAAATTTTTTGATATAGAGTGCAAGGTCTATATGGTA
AAGGTAAGCTACAATATGAAGCCTTACCGCCGGGTCATGATGGAAACCTGGGGTGCGTCAGTTGTACCCAGCCCCAGCCC
GGATACCAAGATAGGCCGTGAAATACTGGAACGTGACCCCGAATGCCCCGGCAGCCTGGGTATTGCTATAAGCGAAGCGG
TGGAAGACGCCGTAACCCATCCCGGCACCAAGTATGCTTTGGGCAGCGTACTTAACCATGTTCTGCTGCATCAGACTATT
ATCGGTCAGGAAGCCAAAAAACAGATGGCTATGGCAGACTGCTACCCGGATATAGTTATTGGCTGCGTAGGCGGCGGCTC
TAACTTCGGCGGTATTGCCCTGCCCTTTGTAGAAGACAAGCTCAATGGCACTCACAAGGATACCCGCATTATGGCTGTTG
AACCTGCCAGCTGCCCTTCACTGACCAAGGGGCTGGATGCCTATGACTTCGGTGACGAAGCCGGCATGACCCCCATGATG
AAAATGTTTACTCTGGGACATCAGTTTATGCCGCCTCCGGTACATGCCGGCGGTCTGCGCTATCACGGTATGTCTCCTTT
GCTGTCTCACCTCAGCCGCCTGAACCTGATTGAGGCCACAGCGGTCAACCAGATAGAGACCTTTGAGGCAGGCATACAGT
TTGCCCAGACCGAAGGTTTCATAAGCGCACCCGAAACCAACCATGCCATAAGGGCGACTATTGATGAAGCCCTGAAATGC
CGCGAAACCGGCGAAGCCAAGACTATACTCTTCTGCCACAGCGGCCATGGCCACGTAGACATGGCTTCCTATGACCTGTA
CCTGCGGGGCAAGCTGAGCAATTATGAATACCCTGCCGAACTGGTTAAAGAAGCCCAGAAAGGTTTGCCCAAAGTAAGCG
CTTAG

Upstream 100 bases:

>100_bases
GCTAAGGAGGCTTTAGATGGACTGGGGCTGACTGGCAACAGGGAAGTAAATACTAAACCAAGGCAAAACGGATACTAAAA
TAATCTCAAGGAGAACAGCC

Downstream 100 bases:

>100_bases
GCAAATACCTCAAAACTGTTTTCACAAGGGAGGGGCAAAAAACCCCTCCCTTTAGTTTATAAATAATTTCTACACAATTT
CCTGCTATAATAAAACCAAT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAQTKFLLQEKDMPQVWYNIQPDLPVPLPPVLHPGTGQPVTPADMEAIFPMSLIMQEFSPERHIPIPEELLEIYKIWRPT
PLYRAHRLEKALQTPAKIFYKLEDVSPAGSHKPNTALAQAYYNKKAGIKRISTETGAGQWGSSLAMACKFFDIECKVYMV
KVSYNMKPYRRVMMETWGASVVPSPSPDTKIGREILERDPECPGSLGIAISEAVEDAVTHPGTKYALGSVLNHVLLHQTI
IGQEAKKQMAMADCYPDIVIGCVGGGSNFGGIALPFVEDKLNGTHKDTRIMAVEPASCPSLTKGLDAYDFGDEAGMTPMM
KMFTLGHQFMPPPVHAGGLRYHGMSPLLSHLSRLNLIEATAVNQIETFEAGIQFAQTEGFISAPETNHAIRATIDEALKC
RETGEAKTILFCHSGHGHVDMASYDLYLRGKLSNYEYPAELVKEAQKGLPKVSA

Sequences:

>Translated_454_residues
MAQTKFLLQEKDMPQVWYNIQPDLPVPLPPVLHPGTGQPVTPADMEAIFPMSLIMQEFSPERHIPIPEELLEIYKIWRPT
PLYRAHRLEKALQTPAKIFYKLEDVSPAGSHKPNTALAQAYYNKKAGIKRISTETGAGQWGSSLAMACKFFDIECKVYMV
KVSYNMKPYRRVMMETWGASVVPSPSPDTKIGREILERDPECPGSLGIAISEAVEDAVTHPGTKYALGSVLNHVLLHQTI
IGQEAKKQMAMADCYPDIVIGCVGGGSNFGGIALPFVEDKLNGTHKDTRIMAVEPASCPSLTKGLDAYDFGDEAGMTPMM
KMFTLGHQFMPPPVHAGGLRYHGMSPLLSHLSRLNLIEATAVNQIETFEAGIQFAQTEGFISAPETNHAIRATIDEALKC
RETGEAKTILFCHSGHGHVDMASYDLYLRGKLSNYEYPAELVKEAQKGLPKVSA
>Mature_453_residues
AQTKFLLQEKDMPQVWYNIQPDLPVPLPPVLHPGTGQPVTPADMEAIFPMSLIMQEFSPERHIPIPEELLEIYKIWRPTP
LYRAHRLEKALQTPAKIFYKLEDVSPAGSHKPNTALAQAYYNKKAGIKRISTETGAGQWGSSLAMACKFFDIECKVYMVK
VSYNMKPYRRVMMETWGASVVPSPSPDTKIGREILERDPECPGSLGIAISEAVEDAVTHPGTKYALGSVLNHVLLHQTII
GQEAKKQMAMADCYPDIVIGCVGGGSNFGGIALPFVEDKLNGTHKDTRIMAVEPASCPSLTKGLDAYDFGDEAGMTPMMK
MFTLGHQFMPPPVHAGGLRYHGMSPLLSHLSRLNLIEATAVNQIETFEAGIQFAQTEGFISAPETNHAIRATIDEALKCR
ETGEAKTILFCHSGHGHVDMASYDLYLRGKLSNYEYPAELVKEAQKGLPKVSA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=27.8215223097113, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321412, Length=345, Percent_Identity=31.8840579710145, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 50013; Mature: 49882

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTKFLLQEKDMPQVWYNIQPDLPVPLPPVLHPGTGQPVTPADMEAIFPMSLIMQEFSP
CCCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
ERHIPIPEELLEIYKIWRPTPLYRAHRLEKALQTPAKIFYKLEDVSPAGSHKPNTALAQA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCCCCHHHHHH
YYNKKAGIKRISTETGAGQWGSSLAMACKFFDIECKVYMVKVSYNMKPYRRVMMETWGAS
HHHHHCCCEEECCCCCCCCCCHHHHHHHEEEEEEEEEEEEEEECCCCHHHHHHHHHCCCC
VVPSPSPDTKIGREILERDPECPGSLGIAISEAVEDAVTHPGTKYALGSVLNHVLLHQTI
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGQEAKKQMAMADCYPDIVIGCVGGGSNFGGIALPFVEDKLNGTHKDTRIMAVEPASCPS
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECEEHHHCCCCCCCCEEEEECCCCCCH
LTKGLDAYDFGDEAGMTPMMKMFTLGHQFMPPPVHAGGLRYHGMSPLLSHLSRLNLIEAT
HHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHH
AVNQIETFEAGIQFAQTEGFISAPETNHAIRATIDEALKCRETGEAKTILFCHSGHGHVD
HHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
MASYDLYLRGKLSNYEYPAELVKEAQKGLPKVSA
EEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQTKFLLQEKDMPQVWYNIQPDLPVPLPPVLHPGTGQPVTPADMEAIFPMSLIMQEFSP
CCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
ERHIPIPEELLEIYKIWRPTPLYRAHRLEKALQTPAKIFYKLEDVSPAGSHKPNTALAQA
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCCCCHHHHHH
YYNKKAGIKRISTETGAGQWGSSLAMACKFFDIECKVYMVKVSYNMKPYRRVMMETWGAS
HHHHHCCCEEECCCCCCCCCCHHHHHHHEEEEEEEEEEEEEEECCCCHHHHHHHHHCCCC
VVPSPSPDTKIGREILERDPECPGSLGIAISEAVEDAVTHPGTKYALGSVLNHVLLHQTI
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IGQEAKKQMAMADCYPDIVIGCVGGGSNFGGIALPFVEDKLNGTHKDTRIMAVEPASCPS
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECEEHHHCCCCCCCCEEEEECCCCCCH
LTKGLDAYDFGDEAGMTPMMKMFTLGHQFMPPPVHAGGLRYHGMSPLLSHLSRLNLIEAT
HHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHH
AVNQIETFEAGIQFAQTEGFISAPETNHAIRATIDEALKCRETGEAKTILFCHSGHGHVD
HHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEE
MASYDLYLRGKLSNYEYPAELVKEAQKGLPKVSA
EEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]