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The map label for this gene is recG [H]

Identifier: 57233949

GI number: 57233949

Start: 1155331

End: 1157553

Strand: Direct

Name: recG [H]

Synonym: DET1268

Alternate gene names: 57233949

Gene position: 1155331-1157553 (Clockwise)

Preceding gene: 57233958

Following gene: 57233948

Centisome position: 78.61

GC content: 52.54

Gene sequence:

>2223_bases
GTGCTGGCATTTCTGCCCAAACTGGAAAACCAGCCTGCCGGCATACCTGCACCCCGCAAAACAGTCAAGGCAAAGCCCGC
CGCTAAAACTGCCAGACCGAAAACAGACGGCTGCCCACCAGCCCTTGACGGGGATATTTACTTAGTAAAAGGCATAAGTG
CCGCTACCGCCCCCAAATTTCACAAAATGGGCATCAACACAGTACGGGACCTGCTTTATTATTTCCCAAACCGCCATCTG
GATTACAGCCGCCTGAAAAAAATATCCCAGCTGGAAGCAGGGTCTGAACAGACCATAATTGCCAATGTCTGGCAGTCCAA
GGTGAACTATATGGGAGGACGCCGCTCAACCGAGGCGGTACTGGGGGACGATACCGGCAATATGCGGGCGGTCTGGTTTA
ACAACCCCTATATGGTGCGTAACCTGAAACCAAATGCCAGAGTAGTCCTTTCGGGGCGGGTTTCCATATTTAATGGCAGG
CCTGTATTTGAATCCCCCGAATGGGAGGAACTGCCTGACGAAGCTGACCTTATTCATACCGGGCGTTTAGTGCCGGTTTA
CCCCCTGACCGCCGGTCTCCACCAGCGAAGCCTGCGCCGCCTGATGAAAAACTTTATAGATATTTCCACCCCCGGTATTT
CAGATTTTCTGCCGGAAGATACCCTTAAAAGAACCCGCCTGCTGCCGCTTGCCGAAGCTATCCGGCAGGCACATTTTCCT
GATACCGAAGAATTGAAAGACGCCGCCCGCAACAGGCTGGCATTTGATGAGCTGTTTATCCTCCAGCTGGGAGTGCTGGC
TAAGAAAAAACGCTGGCAAGAACAAACCGGACGGGCTTTAAAGATAAACCTGCCCGCTATAGACCATTTTATTTCACAGT
TGCCCTTTAAACTGACTGATGCCCAGACCAAATGTCTGGCTGATATAAAAGCCGATATTTCTAAGAGTGTACCCATGTCC
CGCCTGCTTCAAGGGGAAGTGGGCTCGGGAAAGACCATAGTCGCCGTTATTTCCCTGTTTACTGCCGCTGCCAACGGGCT
TCAGGGAGCATTTATGGCACCTACCGAGATACTGGCTGAACAGCATTTTAAAAGTGTCACCCGCCTGTTTGCCTCTATTG
CCAAAGTGAGCACCCTTCTGGACGGGGTGTATACCTTTGAAGGGTTGCTAGACCGCCCGCTCAGGGTAGCTTTGATGATA
AGCGATATGAAATCCAGCCAGAAAGATATCCTGAAGGAAAAGATTAAAAAAGGCGAGATTGATATAGCCATTGGCACCCA
TGCCCTTATCCAGAAGGAAATCCGCTTCAAGTCCCTGGGGCTGGCTGTTATAGACGAGCAGCACCGCTTCGGGGTGGAAC
AACGCTCTGCCCTTAGGAGCAAGGGGCTGAACCCCCACATACTTATCATGACCGCCACCCCCATACCCCGCACTTTGGCT
TTAACCCTATACGGAGAGCTTGACCTTTCAGTTATAGATGAGCTGCCGCCCGGACGCCAGAGCATAAAAACCCGCTGGCT
GAAACCCGAACAGCGTAACAGCGCTTATAACTTTATACGCAAACAGATTGAGGCAGGACGGCAGGCATTTATAATCTGCC
CTTTGGTAGAAGAATCTGAGGCTATACAGGCCAAAGCCGCCACCGCCGAATATGAAACCCTGTCTTCAGAGGTGTTTCCG
GAATCCAAAGTAGCTTTGCTGCACGGGCGGATGAATGCCGCTGAAAAAGAAAGTATCATGAAGCACTTTAACGAAGGCAA
AATGGACATACTGGTTTCCACCCCGGTTGTAGAGGTGGGTATAGACATACCCAACGCCACCGTAATGCTGGTGGAAAGCG
CCGACCGTTTCGGCCTTTCCCAGCTGCACCAGTTCCGGGGGCGGGTGGGCAGAGGAACAGAACAGAGCTACTGCATGTTT
CTGGCCGAAAATCCGTCTCTGCTGGGGCAGGAACGCCTGTCTATCATAGAAAGCACCCAGGACGGCTTTAAGCTGGCGGA
GGAAGACCTGCGGCTGCGGGGGCCGGGTGAATTTTTCGGCACCCGCCAGAGCGGCCTGCCTGACCTGCGCATGGCCAGCA
TATCTGACGTGGGTTTGCTGGAACAGGCACGCAGGGAAGCTACCCGTTTGTTTGAGTCTGACCCTGAACTAAACCTGCCT
GAAAACAAGGCTTTGGAAGAGGAAATAAGGCGGGTATGGCCCAAAAAGAGCGAGTGGAGCTAG

Upstream 100 bases:

>100_bases
AAATAACCCTGATGACCTGCTTATTTTTCACAAACTGAAACTCTCTGACCCGGGTTATGCCCGAATGACAGAAGATAAAC
GCCGTAAGTGGGTAACCGAG

Downstream 100 bases:

>100_bases
CTGCAAAATGACAGATGAGATTAAACCGGGACAGGTAATATCCTATCTTGATATGTGCCAAGCAGAGTCAGTCAATCTAC
AAAAAGGGATGAATTTCAAA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 740; Mature: 740

Protein sequence:

>740_residues
MLAFLPKLENQPAGIPAPRKTVKAKPAAKTARPKTDGCPPALDGDIYLVKGISAATAPKFHKMGINTVRDLLYYFPNRHL
DYSRLKKISQLEAGSEQTIIANVWQSKVNYMGGRRSTEAVLGDDTGNMRAVWFNNPYMVRNLKPNARVVLSGRVSIFNGR
PVFESPEWEELPDEADLIHTGRLVPVYPLTAGLHQRSLRRLMKNFIDISTPGISDFLPEDTLKRTRLLPLAEAIRQAHFP
DTEELKDAARNRLAFDELFILQLGVLAKKKRWQEQTGRALKINLPAIDHFISQLPFKLTDAQTKCLADIKADISKSVPMS
RLLQGEVGSGKTIVAVISLFTAAANGLQGAFMAPTEILAEQHFKSVTRLFASIAKVSTLLDGVYTFEGLLDRPLRVALMI
SDMKSSQKDILKEKIKKGEIDIAIGTHALIQKEIRFKSLGLAVIDEQHRFGVEQRSALRSKGLNPHILIMTATPIPRTLA
LTLYGELDLSVIDELPPGRQSIKTRWLKPEQRNSAYNFIRKQIEAGRQAFIICPLVEESEAIQAKAATAEYETLSSEVFP
ESKVALLHGRMNAAEKESIMKHFNEGKMDILVSTPVVEVGIDIPNATVMLVESADRFGLSQLHQFRGRVGRGTEQSYCMF
LAENPSLLGQERLSIIESTQDGFKLAEEDLRLRGPGEFFGTRQSGLPDLRMASISDVGLLEQARREATRLFESDPELNLP
ENKALEEEIRRVWPKKSEWS

Sequences:

>Translated_740_residues
MLAFLPKLENQPAGIPAPRKTVKAKPAAKTARPKTDGCPPALDGDIYLVKGISAATAPKFHKMGINTVRDLLYYFPNRHL
DYSRLKKISQLEAGSEQTIIANVWQSKVNYMGGRRSTEAVLGDDTGNMRAVWFNNPYMVRNLKPNARVVLSGRVSIFNGR
PVFESPEWEELPDEADLIHTGRLVPVYPLTAGLHQRSLRRLMKNFIDISTPGISDFLPEDTLKRTRLLPLAEAIRQAHFP
DTEELKDAARNRLAFDELFILQLGVLAKKKRWQEQTGRALKINLPAIDHFISQLPFKLTDAQTKCLADIKADISKSVPMS
RLLQGEVGSGKTIVAVISLFTAAANGLQGAFMAPTEILAEQHFKSVTRLFASIAKVSTLLDGVYTFEGLLDRPLRVALMI
SDMKSSQKDILKEKIKKGEIDIAIGTHALIQKEIRFKSLGLAVIDEQHRFGVEQRSALRSKGLNPHILIMTATPIPRTLA
LTLYGELDLSVIDELPPGRQSIKTRWLKPEQRNSAYNFIRKQIEAGRQAFIICPLVEESEAIQAKAATAEYETLSSEVFP
ESKVALLHGRMNAAEKESIMKHFNEGKMDILVSTPVVEVGIDIPNATVMLVESADRFGLSQLHQFRGRVGRGTEQSYCMF
LAENPSLLGQERLSIIESTQDGFKLAEEDLRLRGPGEFFGTRQSGLPDLRMASISDVGLLEQARREATRLFESDPELNLP
ENKALEEEIRRVWPKKSEWS
>Mature_740_residues
MLAFLPKLENQPAGIPAPRKTVKAKPAAKTARPKTDGCPPALDGDIYLVKGISAATAPKFHKMGINTVRDLLYYFPNRHL
DYSRLKKISQLEAGSEQTIIANVWQSKVNYMGGRRSTEAVLGDDTGNMRAVWFNNPYMVRNLKPNARVVLSGRVSIFNGR
PVFESPEWEELPDEADLIHTGRLVPVYPLTAGLHQRSLRRLMKNFIDISTPGISDFLPEDTLKRTRLLPLAEAIRQAHFP
DTEELKDAARNRLAFDELFILQLGVLAKKKRWQEQTGRALKINLPAIDHFISQLPFKLTDAQTKCLADIKADISKSVPMS
RLLQGEVGSGKTIVAVISLFTAAANGLQGAFMAPTEILAEQHFKSVTRLFASIAKVSTLLDGVYTFEGLLDRPLRVALMI
SDMKSSQKDILKEKIKKGEIDIAIGTHALIQKEIRFKSLGLAVIDEQHRFGVEQRSALRSKGLNPHILIMTATPIPRTLA
LTLYGELDLSVIDELPPGRQSIKTRWLKPEQRNSAYNFIRKQIEAGRQAFIICPLVEESEAIQAKAATAEYETLSSEVFP
ESKVALLHGRMNAAEKESIMKHFNEGKMDILVSTPVVEVGIDIPNATVMLVESADRFGLSQLHQFRGRVGRGTEQSYCMF
LAENPSLLGQERLSIIESTQDGFKLAEEDLRLRGPGEFFGTRQSGLPDLRMASISDVGLLEQARREATRLFESDPELNLP
ENKALEEEIRRVWPKKSEWS

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=708, Percent_Identity=37.5706214689266, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787357, Length=417, Percent_Identity=37.8896882494005, Blast_Score=259, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82672; Mature: 82672

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFLPKLENQPAGIPAPRKTVKAKPAAKTARPKTDGCPPALDGDIYLVKGISAATAPKF
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHCCCHH
HKMGINTVRDLLYYFPNRHLDYSRLKKISQLEAGSEQTIIANVWQSKVNYMGGRRSTEAV
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
LGDDTGNMRAVWFNNPYMVRNLKPNARVVLSGRVSIFNGRPVFESPEWEELPDEADLIHT
ECCCCCCEEEEEECCCEEEEECCCCCEEEEECCEEEECCCCCCCCCCHHHCCCCCCCEEC
GRLVPVYPLTAGLHQRSLRRLMKNFIDISTPGISDFLPEDTLKRTRLLPLAEAIRQAHFP
CCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCC
DTEELKDAARNRLAFDELFILQLGVLAKKKRWQEQTGRALKINLPAIDHFISQLPFKLTD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCEECC
AQTKCLADIKADISKSVPMSRLLQGEVGSGKTIVAVISLFTAAANGLQGAFMAPTEILAE
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
QHFKSVTRLFASIAKVSTLLDGVYTFEGLLDRPLRVALMISDMKSSQKDILKEKIKKGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCE
DIAIGTHALIQKEIRFKSLGLAVIDEQHRFGVEQRSALRSKGLNPHILIMTATPIPRTLA
EEEECHHHHHHHHHHHHHCCCCEECCHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCEEE
LTLYGELDLSVIDELPPGRQSIKTRWLKPEQRNSAYNFIRKQIEAGRQAFIICPLVEESE
EEEECCCCHHHHHCCCCCHHHHHHHCCCCHHCCHHHHHHHHHHHCCCEEEEEEEECCCCH
AIQAKAATAEYETLSSEVFPESKVALLHGRMNAAEKESIMKHFNEGKMDILVSTPVVEVG
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCEEEEE
IDIPNATVMLVESADRFGLSQLHQFRGRVGRGTEQSYCMFLAENPSLLGQERLSIIESTQ
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCC
DGFKLAEEDLRLRGPGEFFGTRQSGLPDLRMASISDVGLLEQARREATRLFESDPELNLP
HHHHHHHHCCEECCCCHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC
ENKALEEEIRRVWPKKSEWS
CCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLAFLPKLENQPAGIPAPRKTVKAKPAAKTARPKTDGCPPALDGDIYLVKGISAATAPKF
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHCCCHH
HKMGINTVRDLLYYFPNRHLDYSRLKKISQLEAGSEQTIIANVWQSKVNYMGGRRSTEAV
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
LGDDTGNMRAVWFNNPYMVRNLKPNARVVLSGRVSIFNGRPVFESPEWEELPDEADLIHT
ECCCCCCEEEEEECCCEEEEECCCCCEEEEECCEEEECCCCCCCCCCHHHCCCCCCCEEC
GRLVPVYPLTAGLHQRSLRRLMKNFIDISTPGISDFLPEDTLKRTRLLPLAEAIRQAHFP
CCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCC
DTEELKDAARNRLAFDELFILQLGVLAKKKRWQEQTGRALKINLPAIDHFISQLPFKLTD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCEECC
AQTKCLADIKADISKSVPMSRLLQGEVGSGKTIVAVISLFTAAANGLQGAFMAPTEILAE
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
QHFKSVTRLFASIAKVSTLLDGVYTFEGLLDRPLRVALMISDMKSSQKDILKEKIKKGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHCCCE
DIAIGTHALIQKEIRFKSLGLAVIDEQHRFGVEQRSALRSKGLNPHILIMTATPIPRTLA
EEEECHHHHHHHHHHHHHCCCCEECCHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCEEE
LTLYGELDLSVIDELPPGRQSIKTRWLKPEQRNSAYNFIRKQIEAGRQAFIICPLVEESE
EEEECCCCHHHHHCCCCCHHHHHHHCCCCHHCCHHHHHHHHHHHCCCEEEEEEEECCCCH
AIQAKAATAEYETLSSEVFPESKVALLHGRMNAAEKESIMKHFNEGKMDILVSTPVVEVG
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCEEEEE
IDIPNATVMLVESADRFGLSQLHQFRGRVGRGTEQSYCMFLAENPSLLGQERLSIIESTQ
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCC
DGFKLAEEDLRLRGPGEFFGTRQSGLPDLRMASISDVGLLEQARREATRLFESDPELNLP
HHHHHHHHCCEECCCCHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCC
ENKALEEEIRRVWPKKSEWS
CCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]