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The map label for this gene is gltX [H]

Identifier: 57233878

GI number: 57233878

Start: 1235401

End: 1236855

Strand: Direct

Name: gltX [H]

Synonym: DET1365

Alternate gene names: 57233878

Gene position: 1235401-1236855 (Clockwise)

Preceding gene: 57233879

Following gene: 57233876

Centisome position: 84.06

GC content: 52.03

Gene sequence:

>1455_bases
ATGACAAACGAAGTAAGAGTACGCTACGCCCCCAGTCCCACCGGCTACCCCCATCTGGGCAATATCCGCACTGCTATGTT
CAACTGGCTCTTTGCCAGACACAATGGCGGCAAGTTTATTGTCCGGATAGAAGATACTGACCGCGAACGCTACGTGGAAG
GGGCGGTGGAATCTATACTGGAAAGCCTGAACTGGCTGGGGCTTGACTGGGACGAAGGCCCGGACAAAGGCGGAGACTAC
GGCCCGTACTATCAGTCTGAAAGGCTTTTCCTTTACCGCCAGGCGGCTGAAAGGCTGGTTACCGAGGGCAAAGCCTACTA
CTGCCATTGTTCTTCCGAAAGGCTGGATAAAATGCGGGAAGAGCAGATAGCCCGCAAAGAACCCCCGGGTTATGACCGCT
GCTGCCGTGATTTATGCCTGGGACAAAAAGAGGGGGCGGTTATCCGCTTTAAAATACCCCTTGAAGGACAGACCACATTT
ATAGACCTGATACGCGGTGAAGTAACCTTTGACAACGCCAAACAGGATGACTTTGTTATCTTGAAATCAGACGGCTTTCC
CACCTACCATCTGGCCAGTGTGGTAGATGACCATGCCATGCAGATAAGCCATGTCCTGCGGGCGGAAGAATGGCTACCCT
CCACTCCCAAGCACCTTATGCTGTATAAGGCACTGGGCTATACCCCACCCCAATATGCCCATTTGCCCATGATACTGGGA
CCTGACCGCTCCAAACTTTCCAAGCGGCACGGGGCAACTTCTACTATAGAATACAAACAGGCAGGCTACCTGCCTGAAAC
TATGGTAAATTTCTTGTCCCTGCTAGGCTGGGCTTATGATGACAAGACCGAACTATTCAGCCGGAAGCAGCTGATAGAGT
ATTTCTGTCTGGAAAAGGTAAGCAAGACTGCCGCCATTTTCAACTATGAAAAGCTGGACTGGATGAACGGCATGTATATC
CGTACCCTTTCCGCCCCGGACCTTGCCAGCCGGGCTATACCCTTTCTGGAAAAAGACGAACGGATTGCCGCATCCGGGCG
TTTAAATTTTGACTATACCACCAAAGTAATGCCCCTGATACAGGAAAGGGTCAAAAAACTAAGTGAGCTGGCAGAGCTTT
GCTGGTTTATCTATTCGGACAATATCAGCTATGACCCGGCTTCGCTTATTGATAAGAAACTGACCAAAGATGAAAGCCTC
TGTGCCTTGAAAGCGGCCTTTGCCCGGCTGGAAGCCCTGGCAAACTTTGATGCCGTAAGTATGGAGGAACATATCCGTCC
GCTGGCTGCCGAACTGGAACTAAAACCGGGGCAGCTATTCGGTATGCTCCGCACCGCATCCACCGGCCAGCAGGTTGCCC
CTCCCCTTTTTCAGACAATGGAGGTGCTGGGCAGGGAAAGATGCCTGGGGCGGATAGCCATGGCTATTGCCAGACTGTCT
GAGCTGCCTTCTTAG

Upstream 100 bases:

>100_bases
TGCCCAAACCCCCTGAAGTGACCGCCCCCCAGGCTACCTGAAAATAAATATTTAAACAGCCGCAATACCCGGCAGGCTGT
ACTTATAAGGCAGGAACAAG

Downstream 100 bases:

>100_bases
CGGAGTAAAACAGATAGCAAAGGGTCAGGCCAAAAATGCTCCTGCCGGCTGTCATTTTGGATGCCTGCCGCCAAGAAAGC
GGGCTAAAAAGCATGGCCGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTNEVRVRYAPSPTGYPHLGNIRTAMFNWLFARHNGGKFIVRIEDTDRERYVEGAVESILESLNWLGLDWDEGPDKGGDY
GPYYQSERLFLYRQAAERLVTEGKAYYCHCSSERLDKMREEQIARKEPPGYDRCCRDLCLGQKEGAVIRFKIPLEGQTTF
IDLIRGEVTFDNAKQDDFVILKSDGFPTYHLASVVDDHAMQISHVLRAEEWLPSTPKHLMLYKALGYTPPQYAHLPMILG
PDRSKLSKRHGATSTIEYKQAGYLPETMVNFLSLLGWAYDDKTELFSRKQLIEYFCLEKVSKTAAIFNYEKLDWMNGMYI
RTLSAPDLASRAIPFLEKDERIAASGRLNFDYTTKVMPLIQERVKKLSELAELCWFIYSDNISYDPASLIDKKLTKDESL
CALKAAFARLEALANFDAVSMEEHIRPLAAELELKPGQLFGMLRTASTGQQVAPPLFQTMEVLGRERCLGRIAMAIARLS
ELPS

Sequences:

>Translated_484_residues
MTNEVRVRYAPSPTGYPHLGNIRTAMFNWLFARHNGGKFIVRIEDTDRERYVEGAVESILESLNWLGLDWDEGPDKGGDY
GPYYQSERLFLYRQAAERLVTEGKAYYCHCSSERLDKMREEQIARKEPPGYDRCCRDLCLGQKEGAVIRFKIPLEGQTTF
IDLIRGEVTFDNAKQDDFVILKSDGFPTYHLASVVDDHAMQISHVLRAEEWLPSTPKHLMLYKALGYTPPQYAHLPMILG
PDRSKLSKRHGATSTIEYKQAGYLPETMVNFLSLLGWAYDDKTELFSRKQLIEYFCLEKVSKTAAIFNYEKLDWMNGMYI
RTLSAPDLASRAIPFLEKDERIAASGRLNFDYTTKVMPLIQERVKKLSELAELCWFIYSDNISYDPASLIDKKLTKDESL
CALKAAFARLEALANFDAVSMEEHIRPLAAELELKPGQLFGMLRTASTGQQVAPPLFQTMEVLGRERCLGRIAMAIARLS
ELPS
>Mature_483_residues
TNEVRVRYAPSPTGYPHLGNIRTAMFNWLFARHNGGKFIVRIEDTDRERYVEGAVESILESLNWLGLDWDEGPDKGGDYG
PYYQSERLFLYRQAAERLVTEGKAYYCHCSSERLDKMREEQIARKEPPGYDRCCRDLCLGQKEGAVIRFKIPLEGQTTFI
DLIRGEVTFDNAKQDDFVILKSDGFPTYHLASVVDDHAMQISHVLRAEEWLPSTPKHLMLYKALGYTPPQYAHLPMILGP
DRSKLSKRHGATSTIEYKQAGYLPETMVNFLSLLGWAYDDKTELFSRKQLIEYFCLEKVSKTAAIFNYEKLDWMNGMYIR
TLSAPDLASRAIPFLEKDERIAASGRLNFDYTTKVMPLIQERVKKLSELAELCWFIYSDNISYDPASLIDKKLTKDESLC
ALKAAFARLEALANFDAVSMEEHIRPLAAELELKPGQLFGMLRTASTGQQVAPPLFQTMEVLGRERCLGRIAMAIARLSE
LPS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=489, Percent_Identity=35.9918200408998, Blast_Score=291, Evalue=8e-79,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=40.4166666666667, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI226510925, Length=288, Percent_Identity=34.7222222222222, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=37.7319587628866, Blast_Score=290, Evalue=9e-79,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=34.1317365269461, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6324742, Length=142, Percent_Identity=34.5070422535211, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321192, Length=235, Percent_Identity=27.2340425531915, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=36.5656565656566, Blast_Score=315, Evalue=4e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55257; Mature: 55126

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEVRVRYAPSPTGYPHLGNIRTAMFNWLFARHNGGKFIVRIEDTDRERYVEGAVESIL
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEECCCHHHHHHHHHHHHH
ESLNWLGLDWDEGPDKGGDYGPYYQSERLFLYRQAAERLVTEGKAYYCHCSSERLDKMRE
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
EQIARKEPPGYDRCCRDLCLGQKEGAVIRFKIPLEGQTTFIDLIRGEVTFDNAKQDDFVI
HHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEHHCEEEECCCCCCCEEE
LKSDGFPTYHLASVVDDHAMQISHVLRAEEWLPSTPKHLMLYKALGYTPPQYAHLPMILG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEC
PDRSKLSKRHGATSTIEYKQAGYLPETMVNFLSLLGWAYDDKTELFSRKQLIEYFCLEKV
CCHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SKTAAIFNYEKLDWMNGMYIRTLSAPDLASRAIPFLEKDERIAASGRLNFDYTTKVMPLI
HHHHHHHCHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHH
QERVKKLSELAELCWFIYSDNISYDPASLIDKKLTKDESLCALKAAFARLEALANFDAVS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
MEEHIRPLAAELELKPGQLFGMLRTASTGQQVAPPLFQTMEVLGRERCLGRIAMAIARLS
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELPS
HCCC
>Mature Secondary Structure 
TNEVRVRYAPSPTGYPHLGNIRTAMFNWLFARHNGGKFIVRIEDTDRERYVEGAVESIL
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCEEEEEEECCCHHHHHHHHHHHHH
ESLNWLGLDWDEGPDKGGDYGPYYQSERLFLYRQAAERLVTEGKAYYCHCSSERLDKMRE
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
EQIARKEPPGYDRCCRDLCLGQKEGAVIRFKIPLEGQTTFIDLIRGEVTFDNAKQDDFVI
HHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEHHCEEEECCCCCCCEEE
LKSDGFPTYHLASVVDDHAMQISHVLRAEEWLPSTPKHLMLYKALGYTPPQYAHLPMILG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEC
PDRSKLSKRHGATSTIEYKQAGYLPETMVNFLSLLGWAYDDKTELFSRKQLIEYFCLEKV
CCHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SKTAAIFNYEKLDWMNGMYIRTLSAPDLASRAIPFLEKDERIAASGRLNFDYTTKVMPLI
HHHHHHHCHHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHH
QERVKKLSELAELCWFIYSDNISYDPASLIDKKLTKDESLCALKAAFARLEALANFDAVS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
MEEHIRPLAAELELKPGQLFGMLRTASTGQQVAPPLFQTMEVLGRERCLGRIAMAIARLS
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELPS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA