Click here to switch to the map view.

The map label for this gene is trpF

Identifier: 57233822

GI number: 57233822

Start: 1343035

End: 1343694

Strand: Direct

Name: trpF

Synonym: DET1485

Alternate gene names: 57233822

Gene position: 1343035-1343694 (Clockwise)

Preceding gene: 57233823

Following gene: 57233821

Centisome position: 91.38

GC content: 50.15

Gene sequence:

>660_bases
GTGATTAAGACTAAGATTTGCGGCTTGACAGAGGTTGGACAAGCCTTGGCCACAGCCCGGACAGGGGCTGACTTTGCCGG
AGTGGTATTTGCCGAAAGCAAACGGCGCATCACCACGGAGAAAGCACTTGAGATAGCCGAAGCCCTGAAACCACTAAACC
CAAGACCGATGCTGGTAGGCGTCTTTGCCAACCAGACGGCAGAAGAAGTAAACCGGATTGCCTCCGTCTGCCGTCTGGAC
AGGGTACAGCTAAGCGGTAACGAAAGCTGGGAATACTGCAATCAGGTAAACCTGCCGGTTATCAAGGTTATTCACGTTGC
CGAAGGCACTACCGCCGAACTGGTTATTCAGGAAATACAGGCCGGGCTTAAAGCCCTGAAAAAAACACCCGTATTCCTGC
TGGATACCCATACCAAGGCCTTATTCGGAGGCAGCGGTCAAAGTTTTGACTGGCAGATTGTAAAACAGGTCTCTCTGAAA
TACCCCGTCATGGTGGCCGGCGGACTTAATCCGGAAAACATACAGGGATTTATAAAAATAGCCAAACCATGGGGAATAGA
CGTTGCCAGCGGAGTGGAAACAGACGGGATAAAAGATACAGCCAAAATACACCTTTTCATTGAACGGGTGAAAGATGCGG
ATGGTAACCGGATATGTTAG

Upstream 100 bases:

>100_bases
CAGATATTTCCCGTCTGGAAGAACTGGGAGTAGATGCAGTGCTGGTAGGAGAAGCCTTAACAGCCTCGGTTGATATTGCC
GCCAAAATGAGGGAACTGCT

Downstream 100 bases:

>100_bases
TAATAATGAAAAACGATGCAACTCAGGAACAGATAGATAACGTCATCCGTGAGATTACGGATGCAGGCTACCGGGGCATA
CCCATACCGGGTGACCACCG

Product: N-(5'phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MIKTKICGLTEVGQALATARTGADFAGVVFAESKRRITTEKALEIAEALKPLNPRPMLVGVFANQTAEEVNRIASVCRLD
RVQLSGNESWEYCNQVNLPVIKVIHVAEGTTAELVIQEIQAGLKALKKTPVFLLDTHTKALFGGSGQSFDWQIVKQVSLK
YPVMVAGGLNPENIQGFIKIAKPWGIDVASGVETDGIKDTAKIHLFIERVKDADGNRIC

Sequences:

>Translated_219_residues
MIKTKICGLTEVGQALATARTGADFAGVVFAESKRRITTEKALEIAEALKPLNPRPMLVGVFANQTAEEVNRIASVCRLD
RVQLSGNESWEYCNQVNLPVIKVIHVAEGTTAELVIQEIQAGLKALKKTPVFLLDTHTKALFGGSGQSFDWQIVKQVSLK
YPVMVAGGLNPENIQGFIKIAKPWGIDVASGVETDGIKDTAKIHLFIERVKDADGNRIC
>Mature_219_residues
MIKTKICGLTEVGQALATARTGADFAGVVFAESKRRITTEKALEIAEALKPLNPRPMLVGVFANQTAEEVNRIASVCRLD
RVQLSGNESWEYCNQVNLPVIKVIHVAEGTTAELVIQEIQAGLKALKKTPVFLLDTHTKALFGGSGQSFDWQIVKQVSLK
YPVMVAGGLNPENIQGFIKIAKPWGIDVASGVETDGIKDTAKIHLFIERVKDADGNRIC

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=204, Percent_Identity=34.8039215686275, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320210, Length=224, Percent_Identity=31.6964285714286, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_DEHE1 (Q3Z6G4)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_182185.1
- ProteinModelPortal:   Q3Z6G4
- SMR:   Q3Z6G4
- STRING:   Q3Z6G4
- GeneID:   3229285
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1485
- NMPDR:   fig|243164.3.peg.1410
- TIGR:   DET1485
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- PhylomeDB:   Q3Z6G4
- ProtClustDB:   CLSK836911
- BioCyc:   DETH243164:DET_1485-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 23799; Mature: 23799

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTKICGLTEVGQALATARTGADFAGVVFAESKRRITTEKALEIAEALKPLNPRPMLVG
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
VFANQTAEEVNRIASVCRLDRVQLSGNESWEYCNQVNLPVIKVIHVAEGTTAELVIQEIQ
EECCCCHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHH
AGLKALKKTPVFLLDTHTKALFGGSGQSFDWQIVKQVSLKYPVMVAGGLNPENIQGFIKI
HHHHHHHCCCEEEEECCCCEEECCCCCCCHHHHHHHHCCCCCEEEECCCCHHHCEEEEEE
AKPWGIDVASGVETDGIKDTAKIHLFIERVKDADGNRIC
ECCCCCCCCCCCCCCCCCCHHHHHHEEEEHHCCCCCCCC
>Mature Secondary Structure
MIKTKICGLTEVGQALATARTGADFAGVVFAESKRRITTEKALEIAEALKPLNPRPMLVG
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEE
VFANQTAEEVNRIASVCRLDRVQLSGNESWEYCNQVNLPVIKVIHVAEGTTAELVIQEIQ
EECCCCHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHHH
AGLKALKKTPVFLLDTHTKALFGGSGQSFDWQIVKQVSLKYPVMVAGGLNPENIQGFIKI
HHHHHHHCCCEEEEECCCCEEECCCCCCCHHHHHHHHCCCCCEEEECCCCHHHCEEEEEE
AKPWGIDVASGVETDGIKDTAKIHLFIERVKDADGNRIC
ECCCCCCCCCCCCCCCCCCHHHHHHEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA