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The map label for this gene is ligT [C]

Identifier: 57233742

GI number: 57233742

Start: 1315300

End: 1315881

Strand: Direct

Name: ligT [C]

Synonym: DET1456

Alternate gene names: 57233742

Gene position: 1315300-1315881 (Clockwise)

Preceding gene: 57233743

Following gene: 57233733

Centisome position: 89.49

GC content: 51.2

Gene sequence:

>582_bases
TTGACCGAAGGACTTATCCGCACCTTTATTGCTATAGAACTGCCGCCGGAGCTAAAAACCAGACTCGCCGGTCTCCGGGA
TGCATTACCCATACCCTCACAAGCTGTCAAATGGGTAAACCCCGAATCAATGCATCTGACTATCAAATTCCTGGGGGATA
CCCCTTTAAGCCAAATACCCAATATCATAAAGGGGCTGGAGGAAGCCGCACAGGTCTGCCACGCCTTTACCCTATCCACC
ACCCGCATAGGGACTTTCCCCTCACTTTCAAGCCCCAAGGTAATCTGGCTGGGGCTGCATGGTGAGATAGAAAAACTAAA
TACCCTCTTTGCGGAGGTTGAAAAAAATATTTCAGGGCTGGGTTTTCCGGCAGAAAAACGGGATTTTGCTCCCCACCTAA
CCCTGGGGCGTCTTAAGGAGGTGGCTGCAAAAAGTGACCTGCAAACCGCCGCCGAATCACTTAAAACCGCACCCCCCTTT
CCTAAAACCAGTTTCAAAGCCTCACATATCTGTCTTTTCCAAAGCCAGCTTTTAGCCGCCGGGCCGGTATACACCAGACT
GGCCCAAATCAGCCTCGCCTAG

Upstream 100 bases:

>100_bases
CCTTGAAACATCTGGCACAAGGCTGTCTTGAGCCGGATATATTGGCAAAGGTAATGCAGCAAAACGCCCTGAACTGGCTG
GGCGAAGGGAAAGACCAATC

Downstream 100 bases:

>100_bases
TCTTGTCAGGATAAGCCTCTTGGAGTATACTCAATCAACTTAAAACAAGGTCTCAGCCGGGAGGTAGCTTCGTGGAGAAT
ACAGGTTTTCCGAAATGTTT

Product: 2'-5' RNA ligase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTEGLIRTFIAIELPPELKTRLAGLRDALPIPSQAVKWVNPESMHLTIKFLGDTPLSQIPNIIKGLEEAAQVCHAFTLST
TRIGTFPSLSSPKVIWLGLHGEIEKLNTLFAEVEKNISGLGFPAEKRDFAPHLTLGRLKEVAAKSDLQTAAESLKTAPPF
PKTSFKASHICLFQSQLLAAGPVYTRLAQISLA

Sequences:

>Translated_193_residues
MTEGLIRTFIAIELPPELKTRLAGLRDALPIPSQAVKWVNPESMHLTIKFLGDTPLSQIPNIIKGLEEAAQVCHAFTLST
TRIGTFPSLSSPKVIWLGLHGEIEKLNTLFAEVEKNISGLGFPAEKRDFAPHLTLGRLKEVAAKSDLQTAAESLKTAPPF
PKTSFKASHICLFQSQLLAAGPVYTRLAQISLA
>Mature_192_residues
TEGLIRTFIAIELPPELKTRLAGLRDALPIPSQAVKWVNPESMHLTIKFLGDTPLSQIPNIIKGLEEAAQVCHAFTLSTT
RIGTFPSLSSPKVIWLGLHGEIEKLNTLFAEVEKNISGLGFPAEKRDFAPHLTLGRLKEVAAKSDLQTAAESLKTAPPFP
KTSFKASHICLFQSQLLAAGPVYTRLAQISLA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21007; Mature: 20876

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGLIRTFIAIELPPELKTRLAGLRDALPIPSQAVKWVNPESMHLTIKFLGDTPLSQIP
CCCHHEEHHHEEECCHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEECCCCHHHHH
NIIKGLEEAAQVCHAFTLSTTRIGTFPSLSSPKVIWLGLHGEIEKLNTLFAEVEKNISGL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
GFPAEKRDFAPHLTLGRLKEVAAKSDLQTAAESLKTAPPFPKTSFKASHICLFQSQLLAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHC
GPVYTRLAQISLA
CHHHHHHHHHCCC
>Mature Secondary Structure 
TEGLIRTFIAIELPPELKTRLAGLRDALPIPSQAVKWVNPESMHLTIKFLGDTPLSQIP
CCHHEEHHHEEECCHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEECCCCHHHHH
NIIKGLEEAAQVCHAFTLSTTRIGTFPSLSSPKVIWLGLHGEIEKLNTLFAEVEKNISGL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCC
GFPAEKRDFAPHLTLGRLKEVAAKSDLQTAAESLKTAPPFPKTSFKASHICLFQSQLLAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHC
GPVYTRLAQISLA
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]