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The map label for this gene is hisS

Identifier: 57233674

GI number: 57233674

Start: 8328

End: 9584

Strand: Direct

Name: hisS

Synonym: DET0006

Alternate gene names: 57233674

Gene position: 8328-9584 (Clockwise)

Preceding gene: 57233677

Following gene: 57233673

Centisome position: 0.57

GC content: 52.67

Gene sequence:

>1257_bases
TTGTACCAGTCACCCAGAGGTACAGAAGATATACTGCCTGAAGACCAGCCTTACTGGCATTTTGTCAGGCAGCAGGCCGC
CCGGATAGCCGCCCTGTACGGTTACCAGCGGACAGATACACCCGTTTTTGAAGATGCCGGGCTGTTTGTGCGGAGTGTTG
GCGAGGGGACAGACATTGTCTCCAAGGAAATGTATACCTTTGAAGACCGGGGCGGAGACAAACTGACTTTGCGTCCTGAA
GGGACTGCCCCCATTTGCCGGGCTTACCTTGAGCATGGCATGCAGACCCGCACCAAGCCGGTCAAGCTTTATTATCTTAG
CTCTATTTTCCGTTATGACCGCCCCCAAGCCGGGCGTTACCGCCAGCACCACCAGTTTGGTTTTGAGGCTATTGGCGAGG
CTGATGCTTCGCTGGATGCCGAAGTTATAGAAATGGCCTGGCGTTTTTACAATCTGCTGGGTATCAATGACCTTTCGCTG
GAGTTAAACAGCATAGGCTGCCGTGAGTGCCGCCCTGCCTATATTTCGGCCCTTAAAGCTTATTACGGGCAGCATGAAGG
AAAGCTTTGTTCAGACTGTAAAACCCGTTTGGATAAAAACACCCTGCGTTTGCTGGACTGCAAGCGGGAGGAATGCCAGT
GCGTGGCTGAAAATGCTCCCCGCAGCGCAGATTACCTTTGCCCGGACTGCCTTGCCCACTATAACCGCCTGAAGGAGTGC
CTGACGGTAGTGGATTTGCCCTTCCATGAAAATTTCCGTCTGGTAAGGGGTCTTGATTACTACAGCCGCACTGTTTTTGA
AATCCAGCCCAGGATAGAGGGTGCCCAGAGTACCATTGGCGGCGGAGGGCGTTATGACGGGCTGATAGAACAGCTTGGCG
GAGAGCCTACCCCGGCCATAGGTTTTGCTACCGGTATTGAGCGGATTATTTTAAACCTGAAGCGGCAGGGTATTACCCCG
CCTCCCTTGCCTTCCCCTTCGGTTTTTCTGGCTTATATGGGAGAGGCCGCCTCTTTAGCTTCAATTGCTTTGGCATCAGA
CCTGCGTAAAGCGGGTATAGGTATATACCAGACATATGCCCAAAAGAGTATTAAGGCTCAGCTCAGGCAGGCCAACAGCC
TGGGTGCAGACTGGGCGGTGATACTGGGTGAAGAAGAACTGAAACAGGGCTGTGCTGTCCTCCGGAATATGAAAGAGGCC
GGTCAGGCAACCATACCCCTTGACCAGCTGATTTGTGAGATAAAAAAACAGATATGA

Upstream 100 bases:

>100_bases
TAAAAGTATAGCGCACTGGCAGCGGTGTGTACAAAATCTGGCGTCATGCCTAACTAAAGGCTATAATAGAGACCACTTAC
AGTTGAAGGAGACAGATAGC

Downstream 100 bases:

>100_bases
CAAAGAATATACTAATTATTGGCGGTTACGGCAATGCCGGCAGCTGTATAGCTATGCTCCTCCTTCAGGAGACAGATGCC
CGCATATTTCTGGGCGGCAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MYQSPRGTEDILPEDQPYWHFVRQQAARIAALYGYQRTDTPVFEDAGLFVRSVGEGTDIVSKEMYTFEDRGGDKLTLRPE
GTAPICRAYLEHGMQTRTKPVKLYYLSSIFRYDRPQAGRYRQHHQFGFEAIGEADASLDAEVIEMAWRFYNLLGINDLSL
ELNSIGCRECRPAYISALKAYYGQHEGKLCSDCKTRLDKNTLRLLDCKREECQCVAENAPRSADYLCPDCLAHYNRLKEC
LTVVDLPFHENFRLVRGLDYYSRTVFEIQPRIEGAQSTIGGGGRYDGLIEQLGGEPTPAIGFATGIERIILNLKRQGITP
PPLPSPSVFLAYMGEAASLASIALASDLRKAGIGIYQTYAQKSIKAQLRQANSLGADWAVILGEEELKQGCAVLRNMKEA
GQATIPLDQLICEIKKQI

Sequences:

>Translated_418_residues
MYQSPRGTEDILPEDQPYWHFVRQQAARIAALYGYQRTDTPVFEDAGLFVRSVGEGTDIVSKEMYTFEDRGGDKLTLRPE
GTAPICRAYLEHGMQTRTKPVKLYYLSSIFRYDRPQAGRYRQHHQFGFEAIGEADASLDAEVIEMAWRFYNLLGINDLSL
ELNSIGCRECRPAYISALKAYYGQHEGKLCSDCKTRLDKNTLRLLDCKREECQCVAENAPRSADYLCPDCLAHYNRLKEC
LTVVDLPFHENFRLVRGLDYYSRTVFEIQPRIEGAQSTIGGGGRYDGLIEQLGGEPTPAIGFATGIERIILNLKRQGITP
PPLPSPSVFLAYMGEAASLASIALASDLRKAGIGIYQTYAQKSIKAQLRQANSLGADWAVILGEEELKQGCAVLRNMKEA
GQATIPLDQLICEIKKQI
>Mature_418_residues
MYQSPRGTEDILPEDQPYWHFVRQQAARIAALYGYQRTDTPVFEDAGLFVRSVGEGTDIVSKEMYTFEDRGGDKLTLRPE
GTAPICRAYLEHGMQTRTKPVKLYYLSSIFRYDRPQAGRYRQHHQFGFEAIGEADASLDAEVIEMAWRFYNLLGINDLSL
ELNSIGCRECRPAYISALKAYYGQHEGKLCSDCKTRLDKNTLRLLDCKREECQCVAENAPRSADYLCPDCLAHYNRLKEC
LTVVDLPFHENFRLVRGLDYYSRTVFEIQPRIEGAQSTIGGGGRYDGLIEQLGGEPTPAIGFATGIERIILNLKRQGITP
PPLPSPSVFLAYMGEAASLASIALASDLRKAGIGIYQTYAQKSIKAQLRQANSLGADWAVILGEEELKQGCAVLRNMKEA
GQATIPLDQLICEIKKQI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=464, Percent_Identity=26.2931034482759, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI15029520, Length=465, Percent_Identity=24.7311827956989, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788861, Length=418, Percent_Identity=42.1052631578947, Blast_Score=323, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71993693, Length=468, Percent_Identity=25.8547008547009, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71993686, Length=468, Percent_Identity=25.8547008547009, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6325290, Length=455, Percent_Identity=24.6153846153846, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24643061, Length=473, Percent_Identity=24.3128964059197, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24643059, Length=493, Percent_Identity=24.1379310344828, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_DEHE1 (Q3ZAI8)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_180761.1
- ProteinModelPortal:   Q3ZAI8
- SMR:   Q3ZAI8
- STRING:   Q3ZAI8
- GeneID:   3229137
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0006
- NMPDR:   fig|243164.3.peg.18
- TIGR:   DET0006
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q3ZAI8
- ProtClustDB:   PRK00037
- BioCyc:   DETH243164:DET_0006-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 46861; Mature: 46861

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQSPRGTEDILPEDQPYWHFVRQQAARIAALYGYQRTDTPVFEDAGLFVRSVGEGTDIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHH
SKEMYTFEDRGGDKLTLRPEGTAPICRAYLEHGMQTRTKPVKLYYLSSIFRYDRPQAGRY
HHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCHHCCCCCEEHHHHHHHHHCCCCCCCCH
RQHHQFGFEAIGEADASLDAEVIEMAWRFYNLLGINDLSLELNSIGCRECRPAYISALKA
HHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHH
YYGQHEGKLCSDCKTRLDKNTLRLLDCKREECQCVAENAPRSADYLCPDCLAHYNRLKEC
HHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LTVVDLPFHENFRLVRGLDYYSRTVFEIQPRIEGAQSTIGGGGRYDGLIEQLGGEPTPAI
HHHHCCCCCCCCHHHHCCHHHHHEEEEECCCCCCHHHCCCCCCHHHHHHHHHCCCCCCCH
GFATGIERIILNLKRQGITPPPLPSPSVFLAYMGEAASLASIALASDLRKAGIGIYQTYA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
QKSIKAQLRQANSLGADWAVILGEEELKQGCAVLRNMKEAGQATIPLDQLICEIKKQI
HHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MYQSPRGTEDILPEDQPYWHFVRQQAARIAALYGYQRTDTPVFEDAGLFVRSVGEGTDIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHH
SKEMYTFEDRGGDKLTLRPEGTAPICRAYLEHGMQTRTKPVKLYYLSSIFRYDRPQAGRY
HHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCHHCCCCCEEHHHHHHHHHCCCCCCCCH
RQHHQFGFEAIGEADASLDAEVIEMAWRFYNLLGINDLSLELNSIGCRECRPAYISALKA
HHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHH
YYGQHEGKLCSDCKTRLDKNTLRLLDCKREECQCVAENAPRSADYLCPDCLAHYNRLKEC
HHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LTVVDLPFHENFRLVRGLDYYSRTVFEIQPRIEGAQSTIGGGGRYDGLIEQLGGEPTPAI
HHHHCCCCCCCCHHHHCCHHHHHEEEEECCCCCCHHHCCCCCCHHHHHHHHHCCCCCCCH
GFATGIERIILNLKRQGITPPPLPSPSVFLAYMGEAASLASIALASDLRKAGIGIYQTYA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
QKSIKAQLRQANSLGADWAVILGEEELKQGCAVLRNMKEAGQATIPLDQLICEIKKQI
HHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA