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The map label for this gene is fgs [H]

Identifier: 57233655

GI number: 57233655

Start: 16437

End: 17756

Strand: Direct

Name: fgs [H]

Synonym: DET0016

Alternate gene names: 57233655

Gene position: 16437-17756 (Clockwise)

Preceding gene: 57233656

Following gene: 57233644

Centisome position: 1.12

GC content: 53.11

Gene sequence:

>1320_bases
ATGGGGCTGACAGATTATGAATCTGCCACCAGCCGGGCAGCGGCTAATTATGACCTCAGGCGGGTGGTAGAGCTTCTCTT
GAGGCTTCATAGTCCCCATATAGGTATTCCGTCTGTTCATCTGGCCGGTACCAAGGGTAAAGGCAGTACGGCCGCTCTGA
TTGCTTCGGTTATGTCGGCGGCCGGTTACCGTACCGGTCTTTATACCTCACCCCATCTGCTGGATTTCCGCGAACGTATT
CGTCTGGACGGCAAGATGATTTCCAAAGCCCATCTGGTGCGGATTATGGATTACATCAGCCCTATGGTGGAGGAAATGAA
TGCCCGGACCTGTTTGGGTAAAATAACTTCGTTTGAGGTTATGACGGTGCTGGCGTTTGAGTTTTTTCACCGCCAGCAGG
CTTTTTTTCAGGTGATTGAAACCGGGCTGGGCGGCAGACTGGATGCCACCAATGTTATTACCCCCAACCTTTCGGTGCTT
ACCCCTATAAGTCTGGACCATACTGAGGTCTTGGGAGACAGCCTGGCCAAAATATCTGCCGAGAAAGCGGGTATTATTAA
ACATTTTGTGCCGGTGGTTTCTGCTGCCCAAAGACCGGAAGCTATGGCAGTTATCCGTGAGCGTTGCCTTAAAACCGGCT
CAAAACTGATTGAGGTGACTGACAGCTACAAGGCCGAACTCTTAGGTTACACTGCCCAAGGTCAGTCACTCAAACTTAGC
GGCCGTCAGGCTGAATACCGGCTGTGTCTGCCGCTTTTAGGGCATTACCAGCGTCAAAACGCCCTTACCGCCCTGGCATC
TCTGGAAGCGCTGGTAGAAGAGGGCTTTAAAATAAGCAACGAAGATATAGCTGAAGGCTTCGGGCAGGTCAGATGGCCGG
GGCGGTTTAAGGTTATCAAGGGCAAGCCCCTGATGGTTATTGACGGCGCCCACAATCAGGAAGCCGCTGCCGAACTCCTG
CTTTCGCTGCAAGAATATCCCCCGGCCGCCAAAGTAGAAAAAGAAAAAAGGCTGCTGGTCTTCGGCACTTCCTTTGATAA
AGATGCCGGCGCTATGGCGGATGTGCTTTCCGGGTATTTCGGAACAGTGATACTGACCCATTCCCTCCACCCGCGGGCTA
TGAAACCGGATTTGCTCAGGAAAGCCTTTGACGGGCGTGGGCTTAAGGTACTTGAGGCAGACGGCGTGCCTAAAGCCTTG
GAACTGGCAGGCAGGATAGCTCCGGCAGATGGTCTGGTATGTGTTGCCGGCTCTCTCTTTGTTGCCGCCGAGGCTTTAAA
AGGGCAGAGCCAGCCCCGCTCAGGAACTGGCTCTAAGTAA

Upstream 100 bases:

>100_bases
TCCGGTTATCAAAGACCTGAACGTATCAGACGTTTCCTCAGCGGGCAAGGGTTAGGGGGATTTTCTGGAAGAGAGTTACC
GGGCGGCTTTAAATTACATA

Downstream 100 bases:

>100_bases
CTCTATCAGTTGGTTTTTCTTGTCCGGGTAGGGGATAACCTCTTCCTTCAGTTTTTTCCAGGTCTCCGGGCAGCTTTTGT
ATTCTATCCCCAGAAACTGG

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MGLTDYESATSRAAANYDLRRVVELLLRLHSPHIGIPSVHLAGTKGKGSTAALIASVMSAAGYRTGLYTSPHLLDFRERI
RLDGKMISKAHLVRIMDYISPMVEEMNARTCLGKITSFEVMTVLAFEFFHRQQAFFQVIETGLGGRLDATNVITPNLSVL
TPISLDHTEVLGDSLAKISAEKAGIIKHFVPVVSAAQRPEAMAVIRERCLKTGSKLIEVTDSYKAELLGYTAQGQSLKLS
GRQAEYRLCLPLLGHYQRQNALTALASLEALVEEGFKISNEDIAEGFGQVRWPGRFKVIKGKPLMVIDGAHNQEAAAELL
LSLQEYPPAAKVEKEKRLLVFGTSFDKDAGAMADVLSGYFGTVILTHSLHPRAMKPDLLRKAFDGRGLKVLEADGVPKAL
ELAGRIAPADGLVCVAGSLFVAAEALKGQSQPRSGTGSK

Sequences:

>Translated_439_residues
MGLTDYESATSRAAANYDLRRVVELLLRLHSPHIGIPSVHLAGTKGKGSTAALIASVMSAAGYRTGLYTSPHLLDFRERI
RLDGKMISKAHLVRIMDYISPMVEEMNARTCLGKITSFEVMTVLAFEFFHRQQAFFQVIETGLGGRLDATNVITPNLSVL
TPISLDHTEVLGDSLAKISAEKAGIIKHFVPVVSAAQRPEAMAVIRERCLKTGSKLIEVTDSYKAELLGYTAQGQSLKLS
GRQAEYRLCLPLLGHYQRQNALTALASLEALVEEGFKISNEDIAEGFGQVRWPGRFKVIKGKPLMVIDGAHNQEAAAELL
LSLQEYPPAAKVEKEKRLLVFGTSFDKDAGAMADVLSGYFGTVILTHSLHPRAMKPDLLRKAFDGRGLKVLEADGVPKAL
ELAGRIAPADGLVCVAGSLFVAAEALKGQSQPRSGTGSK
>Mature_438_residues
GLTDYESATSRAAANYDLRRVVELLLRLHSPHIGIPSVHLAGTKGKGSTAALIASVMSAAGYRTGLYTSPHLLDFRERIR
LDGKMISKAHLVRIMDYISPMVEEMNARTCLGKITSFEVMTVLAFEFFHRQQAFFQVIETGLGGRLDATNVITPNLSVLT
PISLDHTEVLGDSLAKISAEKAGIIKHFVPVVSAAQRPEAMAVIRERCLKTGSKLIEVTDSYKAELLGYTAQGQSLKLSG
RQAEYRLCLPLLGHYQRQNALTALASLEALVEEGFKISNEDIAEGFGQVRWPGRFKVIKGKPLMVIDGAHNQEAAAELLL
SLQEYPPAAKVEKEKRLLVFGTSFDKDAGAMADVLSGYFGTVILTHSLHPRAMKPDLLRKAFDGRGLKVLEADGVPKALE
LAGRIAPADGLVCVAGSLFVAAEALKGQSQPRSGTGSK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=340, Percent_Identity=30.8823529411765, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI66932984, Length=340, Percent_Identity=30.8823529411765, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1788654, Length=401, Percent_Identity=30.4239401496259, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17553150, Length=333, Percent_Identity=27.6276276276276, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=333, Percent_Identity=27.6276276276276, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=333, Percent_Identity=27.6276276276276, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=434, Percent_Identity=32.0276497695853, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=342, Percent_Identity=31.5789473684211, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322718, Length=286, Percent_Identity=24.1258741258741, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641571, Length=288, Percent_Identity=31.25, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24581568, Length=186, Percent_Identity=31.1827956989247, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47523; Mature: 47391

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTDYESATSRAAANYDLRRVVELLLRLHSPHIGIPSVHLAGTKGKGSTAALIASVMSA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
AGYRTGLYTSPHLLDFRERIRLDGKMISKAHLVRIMDYISPMVEEMNARTCLGKITSFEV
CCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTVLAFEFFHRQQAFFQVIETGLGGRLDATNVITPNLSVLTPISLDHTEVLGDSLAKISA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCCHHHHHHHHHHHHHH
EKAGIIKHFVPVVSAAQRPEAMAVIRERCLKTGSKLIEVTDSYKAELLGYTAQGQSLKLS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEEECCCCEEEEC
GRQAEYRLCLPLLGHYQRQNALTALASLEALVEEGFKISNEDIAEGFGQVRWPGRFKVIK
CCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEC
GKPLMVIDGAHNQEAAAELLLSLQEYPPAAKVEKEKRLLVFGTSFDKDAGAMADVLSGYF
CCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
GTVILTHSLHPRAMKPDLLRKAFDGRGLKVLEADGVPKALELAGRIAPADGLVCVAGSLF
HHEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCEEHHHHHH
VAAEALKGQSQPRSGTGSK
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GLTDYESATSRAAANYDLRRVVELLLRLHSPHIGIPSVHLAGTKGKGSTAALIASVMSA
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
AGYRTGLYTSPHLLDFRERIRLDGKMISKAHLVRIMDYISPMVEEMNARTCLGKITSFEV
CCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTVLAFEFFHRQQAFFQVIETGLGGRLDATNVITPNLSVLTPISLDHTEVLGDSLAKISA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCCHHHHHHHHHHHHHH
EKAGIIKHFVPVVSAAQRPEAMAVIRERCLKTGSKLIEVTDSYKAELLGYTAQGQSLKLS
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEEECCCCEEEEC
GRQAEYRLCLPLLGHYQRQNALTALASLEALVEEGFKISNEDIAEGFGQVRWPGRFKVIK
CCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEC
GKPLMVIDGAHNQEAAAELLLSLQEYPPAAKVEKEKRLLVFGTSFDKDAGAMADVLSGYF
CCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
GTVILTHSLHPRAMKPDLLRKAFDGRGLKVLEADGVPKALELAGRIAPADGLVCVAGSLF
HHEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCEEHHHHHH
VAAEALKGQSQPRSGTGSK
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105929; 9618466 [H]