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The map label for this gene is tgt

Identifier: 57233581

GI number: 57233581

Start: 45042

End: 46220

Strand: Direct

Name: tgt

Synonym: DET0052

Alternate gene names: 57233581

Gene position: 45042-46220 (Clockwise)

Preceding gene: 57233582

Following gene: 57233580

Centisome position: 3.06

GC content: 53.1

Gene sequence:

>1179_bases
TTGGATAAATCCTTTATATTAAATAAAACCAGTCCCCGCAGTTCTGCCCGCCGCGGCCAGCTGCTCACAGCCCATGGCCA
GGTGGAGACGCCTGTGTTCTGCCCGGTAGGCAGCCAGGCCACTGTAAAAACCCTTACCCCCGAAGACCTGAAATCTGTAA
ACGTAAATATGATACTCTCTAATACCTATCACCTGTATCTCCGCCCCGGCATACCCGTGGTTAAAGAGATGGGGGGACTG
CATAAATTTATGAACTGGGACGGGGTTATCCTGACTGACAGCGGTGGCTATCAGATATTTTCACTGGCCAATCTGCGAAA
GCTGGATGAGGGCGGGGTCAGTTTCCGTTCCCATATAGACGGCAGCACCCGTTACATTACCCCCGAAGATGCGGTCAGCT
TCCAGCAGGATTTGGGTTCGGATATTGCCATGGTGCTGGACGAATGTCCCCATTCCGAAGCCTCCGAGAATGAGGTTTTG
GCGGCTATGGAACGCACCCACCAGTGGGCAAAACGCTGTCTTGCCGCCCATACCCTGAAAAGCCAGCACCTTTTTGCCAT
TGTTCAGGGGGGGCTTTCCCCCGTACTCAGGCAACAGTCTGCCGAATATCTGGCTTCGCTGGATTTCCCCGGTTATGCCC
TGGGCGGGCTTTCGTTAGGTGAGCCTAAGGATATTACCTTTGAAACAGTCCGCCATACCCTGCGGTTTCTGCCCGAAAAC
AAGCCCCGCTACCTGATGGGGGTGGGTGCGCCGGAAGATTTGCTGGAAGGGGTAAGCTGCGGGGTAGATATATTTGACTG
TGTACTGCCTACCAGAGTGGCCCGCAACGGGGCTTTTTTCAGCCGCCTGGGCAGGCTTAATATTCGCAATGCCTCTTTTG
CCACCCAAAAAGGGCCGGTTGACCCGGAGTGTAACTGCTATACCTGCCGCAATTATTCGGCGGCCTACCTGCATCACCTG
TTCCGCTGTGAAGAAATACTGGCCTACCGGCTGGCTACTATACATAACATTGCCTTCCTGAGCAACCTTATGCAGGAAGT
TCGTACTTCTATTGAAAAAGACTGTTTTGAAGAGTTTAAAGGGGATTTCCTGACCCGCTATCAGCCTACCAACGAATCTG
TCCGTATTGAGCAGAAACAGAAATGGCTCTCCGCCCGCAGCGGCGAAACCTCTTCCTAG

Upstream 100 bases:

>100_bases
CCGGGATGATGCGGCCGCGGCCGCAGTTATTTTGCAGGATTATCTGGACGAGACTAATCCGCCCTGCTTCCAGCCTTAAA
TATTCAAGGAGCATACACTT

Downstream 100 bases:

>100_bases
ATATTATCTGGCGGCTGTCTTGCGGCAGGGATAAAAAACGGGAGGTCAGGGGGCTGTTTTAACAGTGCTGTTTTCTGTTA
TTATATTTTTATGATAACAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MDKSFILNKTSPRSSARRGQLLTAHGQVETPVFCPVGSQATVKTLTPEDLKSVNVNMILSNTYHLYLRPGIPVVKEMGGL
HKFMNWDGVILTDSGGYQIFSLANLRKLDEGGVSFRSHIDGSTRYITPEDAVSFQQDLGSDIAMVLDECPHSEASENEVL
AAMERTHQWAKRCLAAHTLKSQHLFAIVQGGLSPVLRQQSAEYLASLDFPGYALGGLSLGEPKDITFETVRHTLRFLPEN
KPRYLMGVGAPEDLLEGVSCGVDIFDCVLPTRVARNGAFFSRLGRLNIRNASFATQKGPVDPECNCYTCRNYSAAYLHHL
FRCEEILAYRLATIHNIAFLSNLMQEVRTSIEKDCFEEFKGDFLTRYQPTNESVRIEQKQKWLSARSGETSS

Sequences:

>Translated_392_residues
MDKSFILNKTSPRSSARRGQLLTAHGQVETPVFCPVGSQATVKTLTPEDLKSVNVNMILSNTYHLYLRPGIPVVKEMGGL
HKFMNWDGVILTDSGGYQIFSLANLRKLDEGGVSFRSHIDGSTRYITPEDAVSFQQDLGSDIAMVLDECPHSEASENEVL
AAMERTHQWAKRCLAAHTLKSQHLFAIVQGGLSPVLRQQSAEYLASLDFPGYALGGLSLGEPKDITFETVRHTLRFLPEN
KPRYLMGVGAPEDLLEGVSCGVDIFDCVLPTRVARNGAFFSRLGRLNIRNASFATQKGPVDPECNCYTCRNYSAAYLHHL
FRCEEILAYRLATIHNIAFLSNLMQEVRTSIEKDCFEEFKGDFLTRYQPTNESVRIEQKQKWLSARSGETSS
>Mature_392_residues
MDKSFILNKTSPRSSARRGQLLTAHGQVETPVFCPVGSQATVKTLTPEDLKSVNVNMILSNTYHLYLRPGIPVVKEMGGL
HKFMNWDGVILTDSGGYQIFSLANLRKLDEGGVSFRSHIDGSTRYITPEDAVSFQQDLGSDIAMVLDECPHSEASENEVL
AAMERTHQWAKRCLAAHTLKSQHLFAIVQGGLSPVLRQQSAEYLASLDFPGYALGGLSLGEPKDITFETVRHTLRFLPEN
KPRYLMGVGAPEDLLEGVSCGVDIFDCVLPTRVARNGAFFSRLGRLNIRNASFATQKGPVDPECNCYTCRNYSAAYLHHL
FRCEEILAYRLATIHNIAFLSNLMQEVRTSIEKDCFEEFKGDFLTRYQPTNESVRIEQKQKWLSARSGETSS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=353, Percent_Identity=47.0254957507082, Blast_Score=319, Evalue=3e-87,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=49.5890410958904, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=43.1266846361186, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=225, Percent_Identity=24.8888888888889, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=45.0704225352113, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_DEHE1 (Q3ZAE2)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_180807.1
- HSSP:   P28720
- ProteinModelPortal:   Q3ZAE2
- SMR:   Q3ZAE2
- STRING:   Q3ZAE2
- GeneID:   3229043
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0052
- NMPDR:   fig|243164.3.peg.64
- TIGR:   DET0052
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q3ZAE2
- ProtClustDB:   PRK00112
- BioCyc:   DETH243164:DET_0052-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43750; Mature: 43750

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSFILNKTSPRSSARRGQLLTAHGQVETPVFCPVGSQATVKTLTPEDLKSVNVNMILS
CCCCEEEECCCCCCHHHCCCEEEECCCCCCCEEECCCCCCEEECCCHHHHHHCCEEEEEE
NTYHLYLRPGIPVVKEMGGLHKFMNWDGVILTDSGGYQIFSLANLRKLDEGGVSFRSHID
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEHHHHHHCCCCCHHHHCCC
GSTRYITPEDAVSFQQDLGSDIAMVLDECPHSEASENEVLAAMERTHQWAKRCLAAHTLK
CCCEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SQHLFAIVQGGLSPVLRQQSAEYLASLDFPGYALGGLSLGEPKDITFETVRHTLRFLPEN
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCC
KPRYLMGVGAPEDLLEGVSCGVDIFDCVLPTRVARNGAFFSRLGRLNIRNASFATQKGPV
CCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCCCCCCC
DPECNCYTCRNYSAAYLHHLFRCEEILAYRLATIHNIAFLSNLMQEVRTSIEKDCFEEFK
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDFLTRYQPTNESVRIEQKQKWLSARSGETSS
CCEEEEECCCCCCEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDKSFILNKTSPRSSARRGQLLTAHGQVETPVFCPVGSQATVKTLTPEDLKSVNVNMILS
CCCCEEEECCCCCCHHHCCCEEEECCCCCCCEEECCCCCCEEECCCHHHHHHCCEEEEEE
NTYHLYLRPGIPVVKEMGGLHKFMNWDGVILTDSGGYQIFSLANLRKLDEGGVSFRSHID
CEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEHHHHHHCCCCCHHHHCCC
GSTRYITPEDAVSFQQDLGSDIAMVLDECPHSEASENEVLAAMERTHQWAKRCLAAHTLK
CCCEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SQHLFAIVQGGLSPVLRQQSAEYLASLDFPGYALGGLSLGEPKDITFETVRHTLRFLPEN
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCC
KPRYLMGVGAPEDLLEGVSCGVDIFDCVLPTRVARNGAFFSRLGRLNIRNASFATQKGPV
CCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCCCCCCC
DPECNCYTCRNYSAAYLHHLFRCEEILAYRLATIHNIAFLSNLMQEVRTSIEKDCFEEFK
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GDFLTRYQPTNESVRIEQKQKWLSARSGETSS
CCEEEEECCCCCCEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA