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The map label for this gene is pinE [H]

Identifier: 57233558

GI number: 57233558

Start: 75226

End: 75843

Strand: Direct

Name: pinE [H]

Synonym: DET0076

Alternate gene names: 57233558

Gene position: 75226-75843 (Clockwise)

Preceding gene: 57233559

Following gene: 57233557

Centisome position: 5.12

GC content: 45.95

Gene sequence:

>618_bases
ATGAAAGTAGCTCTATACGCCAGGGTATCAACTAAAGACAAAGAGCAAAACCCGGAGCTGCAGCTCGCAGCGCTTCGTAA
ATACTGTGCCGACAATGGTTGGGAAATCTACCGGGAATATACAGATGAGGCTTCTGCATCAGATTTTACCGGGCGCAAGG
CTTGGACGGCGCTGATGAAGGAGGCCCAACTGAAAAAGTTCAATGTTCTGCTTGTCTGGAAACTTGACCGAGCTTTTAGA
TCTGTGATCCATGCCGTAAATTCCATGCAGATGCTTAATAGCTATAATGTCGGTTTTAAATCTTATATGGACTCCGGCAT
TGATACCACTACTCCAATGGGGAATTTTGTTTTCTCCATAATGACGGCGGCCGCAGAACTGGAGCAATCAACTATAAGAC
AAAGAGTTAATGCCGGAATTGCTTATGCAAAAGAGAATGGCACCAAATCAGGCAAAGCAATCGGTCGGCCGAGAAAGAGT
ATCGACTTCACAAAAGTTTTGGAAGCGTTCAATCGCGTGGAAATGAATTATACCAGGGCAGCCCGGCTTTTAACCGAGCA
AACCGGGGTAAAAGTAACCCCGGGCTATGTTTATAACCAAATCAAGCGAGGTGGCTGA

Upstream 100 bases:

>100_bases
ACTGGAGATGCTTCTATCAGGGGGTGAAACGCTAGGTGTGGACGGATCGGTAGGCGTTTTGTTTTCCATCATGGACCAAC
GAAAAGCAAGGAGCAACTAA

Downstream 100 bases:

>100_bases
TGTTTTTAACAAAGAAAATGCTTTATATGTTTGGGGTGATCTATTTCCTATTGGGGGCTGCGCTTGTTTCCTTATTGGCA
GGTTATAACATTTGGTTCGT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKVALYARVSTKDKEQNPELQLAALRKYCADNGWEIYREYTDEASASDFTGRKAWTALMKEAQLKKFNVLLVWKLDRAFR
SVIHAVNSMQMLNSYNVGFKSYMDSGIDTTTPMGNFVFSIMTAAAELEQSTIRQRVNAGIAYAKENGTKSGKAIGRPRKS
IDFTKVLEAFNRVEMNYTRAARLLTEQTGVKVTPGYVYNQIKRGG

Sequences:

>Translated_205_residues
MKVALYARVSTKDKEQNPELQLAALRKYCADNGWEIYREYTDEASASDFTGRKAWTALMKEAQLKKFNVLLVWKLDRAFR
SVIHAVNSMQMLNSYNVGFKSYMDSGIDTTTPMGNFVFSIMTAAAELEQSTIRQRVNAGIAYAKENGTKSGKAIGRPRKS
IDFTKVLEAFNRVEMNYTRAARLLTEQTGVKVTPGYVYNQIKRGG
>Mature_205_residues
MKVALYARVSTKDKEQNPELQLAALRKYCADNGWEIYREYTDEASASDFTGRKAWTALMKEAQLKKFNVLLVWKLDRAFR
SVIHAVNSMQMLNSYNVGFKSYMDSGIDTTTPMGNFVFSIMTAAAELEQSTIRQRVNAGIAYAKENGTKSGKAIGRPRKS
IDFTKVLEAFNRVEMNYTRAARLLTEQTGVKVTPGYVYNQIKRGG

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=153, Percent_Identity=39.8692810457516, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23152; Mature: 23152

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVALYARVSTKDKEQNPELQLAALRKYCADNGWEIYREYTDEASASDFTGRKAWTALMK
CCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
EAQLKKFNVLLVWKLDRAFRSVIHAVNSMQMLNSYNVGFKSYMDSGIDTTTPMGNFVFSI
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHH
MTAAAELEQSTIRQRVNAGIAYAKENGTKSGKAIGRPRKSIDFTKVLEAFNRVEMNYTRA
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ARLLTEQTGVKVTPGYVYNQIKRGG
HHHHHHHCCCEECCHHHHHHHHCCC
>Mature Secondary Structure
MKVALYARVSTKDKEQNPELQLAALRKYCADNGWEIYREYTDEASASDFTGRKAWTALMK
CCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
EAQLKKFNVLLVWKLDRAFRSVIHAVNSMQMLNSYNVGFKSYMDSGIDTTTPMGNFVFSI
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHH
MTAAAELEQSTIRQRVNAGIAYAKENGTKSGKAIGRPRKSIDFTKVLEAFNRVEMNYTRA
HHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ARLLTEQTGVKVTPGYVYNQIKRGG
HHHHHHHCCCEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]