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The map label for this gene is ywjE [H]

Identifier: 56965651

GI number: 56965651

Start: 4050389

End: 4051585

Strand: Direct

Name: ywjE [H]

Synonym: ABC3893

Alternate gene names: 56965651

Gene position: 4050389-4051585 (Clockwise)

Preceding gene: 56965643

Following gene: 56965657

Centisome position: 94.11

GC content: 46.03

Gene sequence:

>1197_bases
ATGAATATCCTCATTACGATTCTCGCCTTAGTGGTTGTGATTGGCGTTTGGCTTTATTTCGATTACAAACTGGGCCTCAG
AGCACTTCGGAAAAAATCGCAAGCCACTTCAACAGGCCCTCTTGCAAGCAAGATTGAATTGATCACAAATGGTGAAGACT
TTATCGATAGGCTTCTTGAAGACATCCAGAAGGCAAATAAACACATTCACATTCAGTTTTATATTTTTCGCGATGATTAT
ATCGGAAAACGGATGGTAGACACATTGGTCGCTAAGGCGGAGGCGGGGATAGACACTCGTTTGCTCATTGACCAATTTGG
CTGCAAGCTCCGGCGAGGGACAAAAAAGCAGTTACGGGCTGCCGGCGTTGATGTGGCAATAGCAGCAAAAGTGCACGGCC
CGTGGTTTTTCTTTTCCTTGAATCGCCGCAACCACCGCAAGCTTGCTGTCATTGACGGTCATACAAGCTACTTAGGCGGC
TATAACGTCGGCGATGAATATTTAGGGCGCAATCCCTTTTTTGGCTTTTGGCGCGATTTTCATGTCCGTATAACGGGTGA
AGGCAGCCGTGCCATTCAAACACAATTTTTGGCTGACTTTGAAGCGGCGACTGGCAAACATATTGAAAGAAAATCGTTTT
TCCCAGACTTACAAGCGGGTAGCCAAACATTGCAGTTTATAAGCACACAAGGGATGGAGTTTGAACAAACACTGCTTAAG
CTTATTCGTAGCGCTAAAAACAGCTTAATCATCGGCAGCCCTTATTTTATCCCGACACGGGCCGTAATGGATGCCATCAT
TGACGTGGCGCAAAGAGGCGTCTTCGTTTCCTTAATCCTCCCTGAAAAAGCAGATCATCCCCTTGTTAAGCATGGGGCCA
CTCCTTATGTAATTGAAGCGCTACAGGCAGGTGTGCTTGTCTACCACTATTATCGAGGATTTTATCATGTTAAAGCGGTA
GTAGCCGATCAATCTACATGTTTAGTGGGGACGCCGAATATCGATAAACGCAGTTTTCATCTAAACGATGAAATGTCATG
CTTAACCCATGACCAGACATTTATTGAAACAGTTCTCGCTGTGCTCCATGCTGATATGCACGCTTCCATGACTGTTACAA
TCGAACAGCTCCAGAAGCGCCCACTCCTTGACCGTGTGAAAGAAGTTACCGCAGCCCCCCTTTCACCATTGCTGTGA

Upstream 100 bases:

>100_bases
CTTATGAATGAGCATTCAGTCAGATGAACTTTTTTTCTTTTGCATGACCACCCTAATTCAGGAAAAGCTATAGACAATAC
TTGTTGCCAGGAGGTCTTTT

Downstream 100 bases:

>100_bases
CATCACGTTTGAACAGTAACGTACGCCCTAGGATAAAAGGAGCCAACCATAATCGAAAAAGAGTGGCAACCACCATACAA
GGTGGTTGCCACTCTTTCTT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNILITILALVVVIGVWLYFDYKLGLRALRKKSQATSTGPLASKIELITNGEDFIDRLLEDIQKANKHIHIQFYIFRDDY
IGKRMVDTLVAKAEAGIDTRLLIDQFGCKLRRGTKKQLRAAGVDVAIAAKVHGPWFFFSLNRRNHRKLAVIDGHTSYLGG
YNVGDEYLGRNPFFGFWRDFHVRITGEGSRAIQTQFLADFEAATGKHIERKSFFPDLQAGSQTLQFISTQGMEFEQTLLK
LIRSAKNSLIIGSPYFIPTRAVMDAIIDVAQRGVFVSLILPEKADHPLVKHGATPYVIEALQAGVLVYHYYRGFYHVKAV
VADQSTCLVGTPNIDKRSFHLNDEMSCLTHDQTFIETVLAVLHADMHASMTVTIEQLQKRPLLDRVKEVTAAPLSPLL

Sequences:

>Translated_398_residues
MNILITILALVVVIGVWLYFDYKLGLRALRKKSQATSTGPLASKIELITNGEDFIDRLLEDIQKANKHIHIQFYIFRDDY
IGKRMVDTLVAKAEAGIDTRLLIDQFGCKLRRGTKKQLRAAGVDVAIAAKVHGPWFFFSLNRRNHRKLAVIDGHTSYLGG
YNVGDEYLGRNPFFGFWRDFHVRITGEGSRAIQTQFLADFEAATGKHIERKSFFPDLQAGSQTLQFISTQGMEFEQTLLK
LIRSAKNSLIIGSPYFIPTRAVMDAIIDVAQRGVFVSLILPEKADHPLVKHGATPYVIEALQAGVLVYHYYRGFYHVKAV
VADQSTCLVGTPNIDKRSFHLNDEMSCLTHDQTFIETVLAVLHADMHASMTVTIEQLQKRPLLDRVKEVTAAPLSPLL
>Mature_398_residues
MNILITILALVVVIGVWLYFDYKLGLRALRKKSQATSTGPLASKIELITNGEDFIDRLLEDIQKANKHIHIQFYIFRDDY
IGKRMVDTLVAKAEAGIDTRLLIDQFGCKLRRGTKKQLRAAGVDVAIAAKVHGPWFFFSLNRRNHRKLAVIDGHTSYLGG
YNVGDEYLGRNPFFGFWRDFHVRITGEGSRAIQTQFLADFEAATGKHIERKSFFPDLQAGSQTLQFISTQGMEFEQTLLK
LIRSAKNSLIIGSPYFIPTRAVMDAIIDVAQRGVFVSLILPEKADHPLVKHGATPYVIEALQAGVLVYHYYRGFYHVKAV
VADQSTCLVGTPNIDKRSFHLNDEMSCLTHDQTFIETVLAVLHADMHASMTVTIEQLQKRPLLDRVKEVTAAPLSPLL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=374, Percent_Identity=33.6898395721925, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787007, Length=369, Percent_Identity=28.4552845528455, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 44803; Mature: 44803

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILITILALVVVIGVWLYFDYKLGLRALRKKSQATSTGPLASKIELITNGEDFIDRLLE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEECCHHHHHHHHH
DIQKANKHIHIQFYIFRDDYIGKRMVDTLVAKAEAGIDTRLLIDQFGCKLRRGTKKQLRA
HHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCHHHHHH
AGVDVAIAAKVHGPWFFFSLNRRNHRKLAVIDGHTSYLGGYNVGDEYLGRNPFFGFWRDF
CCCCEEEEEEECCCEEEEEECCCCCCEEEEECCCCHHCCCCCCCHHHCCCCCCEECEEEE
HVRITGEGSRAIQTQFLADFEAATGKHIERKSFFPDLQAGSQTLQFISTQGMEFEQTLLK
EEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHH
LIRSAKNSLIIGSPYFIPTRAVMDAIIDVAQRGVFVSLILPEKADHPLVKHGATPYVIEA
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHCCCCHHHHHH
LQAGVLVYHYYRGFYHVKAVVADQSTCLVGTPNIDKRSFHLNDEMSCLTHDQTFIETVLA
HHHHHHHHHHHHHHEEEEEEEECCCEEEEECCCCCCCEEECCCCCHHHCCCHHHHHHHHH
VLHADMHASMTVTIEQLQKRPLLDRVKEVTAAPLSPLL
HHHHCCCCHHEEEHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNILITILALVVVIGVWLYFDYKLGLRALRKKSQATSTGPLASKIELITNGEDFIDRLLE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEECCHHHHHHHHH
DIQKANKHIHIQFYIFRDDYIGKRMVDTLVAKAEAGIDTRLLIDQFGCKLRRGTKKQLRA
HHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCHHHHHH
AGVDVAIAAKVHGPWFFFSLNRRNHRKLAVIDGHTSYLGGYNVGDEYLGRNPFFGFWRDF
CCCCEEEEEEECCCEEEEEECCCCCCEEEEECCCCHHCCCCCCCHHHCCCCCCEECEEEE
HVRITGEGSRAIQTQFLADFEAATGKHIERKSFFPDLQAGSQTLQFISTQGMEFEQTLLK
EEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHH
LIRSAKNSLIIGSPYFIPTRAVMDAIIDVAQRGVFVSLILPEKADHPLVKHGATPYVIEA
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHCCCCHHHHHH
LQAGVLVYHYYRGFYHVKAVVADQSTCLVGTPNIDKRSFHLNDEMSCLTHDQTFIETVLA
HHHHHHHHHHHHHHEEEEEEEECCCEEEEECCCCCCCEEECCCCCHHHCCCHHHHHHHHH
VLHADMHASMTVTIEQLQKRPLLDRVKEVTAAPLSPLL
HHHHCCCCHHEEEHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]