| Definition | Bacillus clausii KSM-K16, complete genome. |
|---|---|
| Accession | NC_006582 |
| Length | 4,303,871 |
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The map label for this gene is dkgB [H]
Identifier: 56965239
GI number: 56965239
Start: 3609435
End: 3610268
Strand: Direct
Name: dkgB [H]
Synonym: ABC3477
Alternate gene names: 56965239
Gene position: 3609435-3610268 (Clockwise)
Preceding gene: 56965228
Following gene: 56965240
Centisome position: 83.86
GC content: 46.16
Gene sequence:
>834_bases ATGATTCCCACTGTCACACTGAAAGATGGGCTGCAGTTGCCAGCTATTGGCTTTGGCACGTATGCGTTGAATGGAGCAGC AGGCGTCAATGCAATGGTGAGCGCGATTGATATTGGCTATCGTCTGCTAGATTCTGCCTTCAACTACGAGAATGAGGGAG CTGTCGGAGAAGCGATTCGGCGTAGTTCTGTCCCAAGGGCACAGTTGCGCGTCACTTCAAAGCTACCAGGGCGGCGACAA GCATATGCGCAAGCGATCGTGACCATTCAAGAATCTTTGTTGCGCGCAAGATTGGACTATTATGACCTATACTTGATCCA TTGGCCAAACCCAAAAACCGACTTATATGTAGAAGCATGGCAAGCATTAATCGATGCCCAAAAATGGGGGCTTATTCGCT CAATTGGAGTATGTAACTTTTTGCCAGAGCATTTAGAGCGATTAGAACGGGAAACAGGCGTCATGCCAAGCATTAACCAA ATTGAACTTCACCCTTATTTTTCTCAGGAAAGCCAACGTGTTTGGGATAACAATCATCATATTGTTACCGAATCATGGAG CCCTCTCGGCCGCGCTAGCAGCGTTTTGCAAGACAAAGTGATTAAACAGATTGCGGCTACGCACAGAAAAAGCGTCTCAC AAATTATATTGCGCTGGCACGTACAGCTTGGGGCAATTCCGTTGCCAAAATCGGCTTCACCTGAACGCCAACTTGAAAAC ATAGCGATTTTCGATTTTTCCCTAAGCACGGAAGAAATGGAGCAAATAAACAAATTAACAAAAGCCGACGGCCGCATTCA AAACCAGGATCCTGCCGAATATGAAGAATTTTAA
Upstream 100 bases:
>100_bases AGCAAAGCGTCAACAGCAATTGAAGTCGCAAACAACCTTTGGTTAGACGTTGATAAACGTTCAATGTTAGACTATTGAAA AAAGACAAGGAGTGTTCCAG
Downstream 100 bases:
>100_bases CGATTAGACAAAAAATGGTAGAGCGGGACGTAACGAATACACGTAGTTTCATAAGCAAGCATATGATCGAGGCAAATGCC GAGCATACCCTTGTCCACAG
Product: 2,5-didehydrogluconate reductase
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 277; Mature: 277
Protein sequence:
>277_residues MIPTVTLKDGLQLPAIGFGTYALNGAAGVNAMVSAIDIGYRLLDSAFNYENEGAVGEAIRRSSVPRAQLRVTSKLPGRRQ AYAQAIVTIQESLLRARLDYYDLYLIHWPNPKTDLYVEAWQALIDAQKWGLIRSIGVCNFLPEHLERLERETGVMPSINQ IELHPYFSQESQRVWDNNHHIVTESWSPLGRASSVLQDKVIKQIAATHRKSVSQIILRWHVQLGAIPLPKSASPERQLEN IAIFDFSLSTEEMEQINKLTKADGRIQNQDPAEYEEF
Sequences:
>Translated_277_residues MIPTVTLKDGLQLPAIGFGTYALNGAAGVNAMVSAIDIGYRLLDSAFNYENEGAVGEAIRRSSVPRAQLRVTSKLPGRRQ AYAQAIVTIQESLLRARLDYYDLYLIHWPNPKTDLYVEAWQALIDAQKWGLIRSIGVCNFLPEHLERLERETGVMPSINQ IELHPYFSQESQRVWDNNHHIVTESWSPLGRASSVLQDKVIKQIAATHRKSVSQIILRWHVQLGAIPLPKSASPERQLEN IAIFDFSLSTEEMEQINKLTKADGRIQNQDPAEYEEF >Mature_277_residues MIPTVTLKDGLQLPAIGFGTYALNGAAGVNAMVSAIDIGYRLLDSAFNYENEGAVGEAIRRSSVPRAQLRVTSKLPGRRQ AYAQAIVTIQESLLRARLDYYDLYLIHWPNPKTDLYVEAWQALIDAQKWGLIRSIGVCNFLPEHLERLERETGVMPSINQ IELHPYFSQESQRVWDNNHHIVTESWSPLGRASSVLQDKVIKQIAATHRKSVSQIILRWHVQLGAIPLPKSASPERQLEN IAIFDFSLSTEEMEQINKLTKADGRIQNQDPAEYEEF
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI223468663, Length=296, Percent_Identity=35.1351351351351, Blast_Score=168, Evalue=5e-42, Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=34.1216216216216, Blast_Score=155, Evalue=3e-38, Organism=Homo sapiens, GI24497577, Length=290, Percent_Identity=32.7586206896552, Blast_Score=155, Evalue=5e-38, Organism=Homo sapiens, GI5174391, Length=290, Percent_Identity=32.7586206896552, Blast_Score=155, Evalue=5e-38, Organism=Homo sapiens, GI291291012, Length=266, Percent_Identity=35.3383458646617, Blast_Score=151, Evalue=6e-37, Organism=Homo sapiens, GI310109922, Length=277, Percent_Identity=35.3790613718412, Blast_Score=144, Evalue=6e-35, Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=30.8474576271186, Blast_Score=144, Evalue=1e-34, Organism=Homo sapiens, GI5453543, Length=296, Percent_Identity=33.7837837837838, Blast_Score=142, Evalue=3e-34, Organism=Homo sapiens, GI310109920, Length=296, Percent_Identity=33.7837837837838, Blast_Score=142, Evalue=4e-34, Organism=Homo sapiens, GI45446745, Length=296, Percent_Identity=33.445945945946, Blast_Score=140, Evalue=9e-34, Organism=Homo sapiens, GI4503285, Length=296, Percent_Identity=33.445945945946, Blast_Score=140, Evalue=9e-34, Organism=Homo sapiens, GI4502049, Length=286, Percent_Identity=33.5664335664336, Blast_Score=140, Evalue=1e-33, Organism=Homo sapiens, GI24497583, Length=295, Percent_Identity=32.5423728813559, Blast_Score=139, Evalue=4e-33, Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=8e-32, Organism=Homo sapiens, GI300116273, Length=278, Percent_Identity=32.0143884892086, Blast_Score=133, Evalue=2e-31, Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=32.258064516129, Blast_Score=127, Evalue=1e-29, Organism=Homo sapiens, GI310109924, Length=144, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI310109926, Length=126, Percent_Identity=38.8888888888889, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI207028673, Length=126, Percent_Identity=38.0952380952381, Blast_Score=72, Evalue=6e-13, Organism=Escherichia coli, GI87082198, Length=274, Percent_Identity=43.0656934306569, Blast_Score=229, Evalue=1e-61, Organism=Escherichia coli, GI1786400, Length=255, Percent_Identity=35.6862745098039, Blast_Score=158, Evalue=4e-40, Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=28.996282527881, Blast_Score=87, Evalue=8e-19, Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=36.8600682593857, Blast_Score=166, Evalue=8e-42, Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=36.3945578231293, Blast_Score=165, Evalue=2e-41, Organism=Caenorhabditis elegans, GI71998625, Length=272, Percent_Identity=31.9852941176471, Blast_Score=150, Evalue=5e-37, Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=33.7037037037037, Blast_Score=149, Evalue=1e-36, Organism=Caenorhabditis elegans, GI17564128, Length=295, Percent_Identity=32.5423728813559, Blast_Score=149, Evalue=2e-36, Organism=Caenorhabditis elegans, GI17561298, Length=276, Percent_Identity=30.4347826086957, Blast_Score=147, Evalue=5e-36, Organism=Caenorhabditis elegans, GI17566692, Length=284, Percent_Identity=32.3943661971831, Blast_Score=142, Evalue=2e-34, Organism=Caenorhabditis elegans, GI17537079, Length=302, Percent_Identity=30.794701986755, Blast_Score=134, Evalue=6e-32, Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=29.1666666666667, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17550248, Length=298, Percent_Identity=31.2080536912752, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=28.7822878228782, Blast_Score=121, Evalue=3e-28, Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=25.0909090909091, Blast_Score=95, Evalue=5e-20, Organism=Saccharomyces cerevisiae, GI6320576, Length=290, Percent_Identity=33.1034482758621, Blast_Score=157, Evalue=1e-39, Organism=Saccharomyces cerevisiae, GI6324694, Length=304, Percent_Identity=33.5526315789474, Blast_Score=157, Evalue=2e-39, Organism=Saccharomyces cerevisiae, GI6321896, Length=318, Percent_Identity=31.1320754716981, Blast_Score=138, Evalue=1e-33, Organism=Saccharomyces cerevisiae, GI6322556, Length=267, Percent_Identity=29.9625468164794, Blast_Score=132, Evalue=7e-32, Organism=Saccharomyces cerevisiae, GI6319625, Length=292, Percent_Identity=30.1369863013699, Blast_Score=119, Evalue=7e-28, Organism=Saccharomyces cerevisiae, GI6320079, Length=279, Percent_Identity=29.7491039426523, Blast_Score=113, Evalue=3e-26, Organism=Drosophila melanogaster, GI21356425, Length=281, Percent_Identity=33.8078291814947, Blast_Score=165, Evalue=3e-41, Organism=Drosophila melanogaster, GI24657054, Length=274, Percent_Identity=34.6715328467153, Blast_Score=164, Evalue=8e-41, Organism=Drosophila melanogaster, GI20129731, Length=283, Percent_Identity=34.6289752650177, Blast_Score=157, Evalue=9e-39, Organism=Drosophila melanogaster, GI24663317, Length=289, Percent_Identity=34.2560553633218, Blast_Score=155, Evalue=4e-38, Organism=Drosophila melanogaster, GI24644950, Length=294, Percent_Identity=30.2721088435374, Blast_Score=142, Evalue=3e-34, Organism=Drosophila melanogaster, GI45553081, Length=306, Percent_Identity=30.3921568627451, Blast_Score=142, Evalue=3e-34, Organism=Drosophila melanogaster, GI24662789, Length=304, Percent_Identity=30.9210526315789, Blast_Score=135, Evalue=4e-32, Organism=Drosophila melanogaster, GI221378297, Length=314, Percent_Identity=28.6624203821656, Blast_Score=134, Evalue=5e-32, Organism=Drosophila melanogaster, GI24662785, Length=278, Percent_Identity=33.8129496402878, Blast_Score=119, Evalue=3e-27, Organism=Drosophila melanogaster, GI24662781, Length=279, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=3e-27, Organism=Drosophila melanogaster, GI281366140, Length=269, Percent_Identity=29.7397769516729, Blast_Score=114, Evalue=8e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 31321; Mature: 31321
Theoretical pI: Translated: 6.41; Mature: 6.41
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIPTVTLKDGLQLPAIGFGTYALNGAAGVNAMVSAIDIGYRLLDSAFNYENEGAVGEAIR CCCCEECCCCCCCCCEECCHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH RSSVPRAQLRVTSKLPGRRQAYAQAIVTIQESLLRARLDYYDLYLIHWPNPKTDLYVEAW HCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHH QALIDAQKWGLIRSIGVCNFLPEHLERLERETGVMPSINQIELHPYFSQESQRVWDNNHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHCCCCE IVTESWSPLGRASSVLQDKVIKQIAATHRKSVSQIILRWHVQLGAIPLPKSASPERQLEN EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCE IAIFDFSLSTEEMEQINKLTKADGRIQNQDPAEYEEF EEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHCCC >Mature Secondary Structure MIPTVTLKDGLQLPAIGFGTYALNGAAGVNAMVSAIDIGYRLLDSAFNYENEGAVGEAIR CCCCEECCCCCCCCCEECCHHEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH RSSVPRAQLRVTSKLPGRRQAYAQAIVTIQESLLRARLDYYDLYLIHWPNPKTDLYVEAW HCCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHH QALIDAQKWGLIRSIGVCNFLPEHLERLERETGVMPSINQIELHPYFSQESQRVWDNNHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHCCCCE IVTESWSPLGRASSVLQDKVIKQIAATHRKSVSQIILRWHVQLGAIPLPKSASPERQLEN EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCE IAIFDFSLSTEEMEQINKLTKADGRIQNQDPAEYEEF EEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA