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The map label for this gene is ykfA [H]

Identifier: 56965100

GI number: 56965100

Start: 3464301

End: 3465221

Strand: Direct

Name: ykfA [H]

Synonym: ABC3338

Alternate gene names: 56965100

Gene position: 3464301-3465221 (Clockwise)

Preceding gene: 56965099

Following gene: 56965101

Centisome position: 80.49

GC content: 48.21

Gene sequence:

>921_bases
ATGGCCCAAAAACCACCGCGTTTACAGCAAGGGGATGCAGTTGGGATCGTCGCTCTTGGTAGCCCCCGCTCTGCTGATGC
GATAAATGCGAATGTTGCTTACTTAAGGGGGCTCGGCTTCCAGCCTGTGCTCGGCGAACATGTCTATGCCCGTACCGGAT
TTTTAGCTGGTCCGCCACAAGACCGCGCCAGTGATTTTATGACGATGATCGAAGACCCTTCGATTAAATGGATTTTGCCG
ACACGAGGCGGCGTAGGCGTTGCTGGCATTTTTCCATACCTTGATTTTGATACCATACGCGCCAATCCAAAAATCATTTC
AGGCTATAGCGACATTACGATTTTATTAAACGTGCTTTACCAGGAAGCAGACTTGATTGGCTTCCATAGTTTGCTTTTGC
TTGATTTTCACAACGGCACGCCTGACTACAATTTCAACCAGTTCTTCTCGTCAACCATGATGCCATCAAGCCCACGCCTT
CTAGAAAACCCACCTGGCTTCCCGCTTAACGGCCTCGTTAATGGAAGAGGAAGAGGCCCGCTTGTTGGTGGCAACCTCAC
CTCTTTTGTTGACAGCCTTGGCACGCCTTATGAAATTGATACAGCCGGAAAAGTCATTTTTTTAGAAGATACGCATGAAC
CAGTCAATACCGTCTACCGCTATTTGGCACACCTCGAAGCTGCTGGCAAATTCGCTGATTGCGAAGCGATTATTATGGGC
ACATGCACGAGCTGCCCGATCGCCTACAACACAAGCTATGACGAATTGATTTCCGGTTTTCTTGTGCCTTTAGGCAAACC
ACTTGTCACGAATCTTCAATCAGGACATGGTTACTACAAAGCCAGTGTGCCAATCGGAGCAGAAGTAGAAGTAGACGGAA
CAAACGGGCGGATTACGATTTTGGAAAATACCGTGAGTTAA

Upstream 100 bases:

>100_bases
TTCGCTTGTTTGGATGTAAATGGTCGTGATTTGCTGTTCTTTGTGCAGGCCTCCTCCACATATACATGCAAAAGCAAGGA
CGCCGCAAAGGAGGTATACT

Downstream 100 bases:

>100_bases
ACTATCCCCATTTCAACAAATGGTTTGAAGGAAGCTTATGCCTCAAAATGGGGCGAGCTTCTTTTTCCACTTTCTCTACT
TCCGCCTCTATCTAAAACAG

Product: carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MAQKPPRLQQGDAVGIVALGSPRSADAINANVAYLRGLGFQPVLGEHVYARTGFLAGPPQDRASDFMTMIEDPSIKWILP
TRGGVGVAGIFPYLDFDTIRANPKIISGYSDITILLNVLYQEADLIGFHSLLLLDFHNGTPDYNFNQFFSSTMMPSSPRL
LENPPGFPLNGLVNGRGRGPLVGGNLTSFVDSLGTPYEIDTAGKVIFLEDTHEPVNTVYRYLAHLEAAGKFADCEAIIMG
TCTSCPIAYNTSYDELISGFLVPLGKPLVTNLQSGHGYYKASVPIGAEVEVDGTNGRITILENTVS

Sequences:

>Translated_306_residues
MAQKPPRLQQGDAVGIVALGSPRSADAINANVAYLRGLGFQPVLGEHVYARTGFLAGPPQDRASDFMTMIEDPSIKWILP
TRGGVGVAGIFPYLDFDTIRANPKIISGYSDITILLNVLYQEADLIGFHSLLLLDFHNGTPDYNFNQFFSSTMMPSSPRL
LENPPGFPLNGLVNGRGRGPLVGGNLTSFVDSLGTPYEIDTAGKVIFLEDTHEPVNTVYRYLAHLEAAGKFADCEAIIMG
TCTSCPIAYNTSYDELISGFLVPLGKPLVTNLQSGHGYYKASVPIGAEVEVDGTNGRITILENTVS
>Mature_305_residues
AQKPPRLQQGDAVGIVALGSPRSADAINANVAYLRGLGFQPVLGEHVYARTGFLAGPPQDRASDFMTMIEDPSIKWILPT
RGGVGVAGIFPYLDFDTIRANPKIISGYSDITILLNVLYQEADLIGFHSLLLLDFHNGTPDYNFNQFFSSTMMPSSPRLL
ENPPGFPLNGLVNGRGRGPLVGGNLTSFVDSLGTPYEIDTAGKVIFLEDTHEPVNTVYRYLAHLEAAGKFADCEAIIMGT
CTSCPIAYNTSYDELISGFLVPLGKPLVTNLQSGHGYYKASVPIGAEVEVDGTNGRITILENTVS

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=288, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 32946; Mature: 32815

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKPPRLQQGDAVGIVALGSPRSADAINANVAYLRGLGFQPVLGEHVYARTGFLAGPPQ
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCC
DRASDFMTMIEDPSIKWILPTRGGVGVAGIFPYLDFDTIRANPKIISGYSDITILLNVLY
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCEEECCCEEEECCHHHHHHHHHHH
QEADLIGFHSLLLLDFHNGTPDYNFNQFFSSTMMPSSPRLLENPPGFPLNGLVNGRGRGP
HHHHHHEEEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCC
LVGGNLTSFVDSLGTPYEIDTAGKVIFLEDTHEPVNTVYRYLAHLEAAGKFADCEAIIMG
EECCCHHHHHHHCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
TCTSCPIAYNTSYDELISGFLVPLGKPLVTNLQSGHGYYKASVPIGAEVEVDGTNGRITI
CCCCCCEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCEEEE
LENTVS
EECCCC
>Mature Secondary Structure 
AQKPPRLQQGDAVGIVALGSPRSADAINANVAYLRGLGFQPVLGEHVYARTGFLAGPPQ
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCC
DRASDFMTMIEDPSIKWILPTRGGVGVAGIFPYLDFDTIRANPKIISGYSDITILLNVLY
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCEEECCCEEEECCHHHHHHHHHHH
QEADLIGFHSLLLLDFHNGTPDYNFNQFFSSTMMPSSPRLLENPPGFPLNGLVNGRGRGP
HHHHHHEEEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCC
LVGGNLTSFVDSLGTPYEIDTAGKVIFLEDTHEPVNTVYRYLAHLEAAGKFADCEAIIMG
EECCCHHHHHHHCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEE
TCTSCPIAYNTSYDELISGFLVPLGKPLVTNLQSGHGYYKASVPIGAEVEVDGTNGRITI
CCCCCCEEECCCHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCEEEEECCCCEEEE
LENTVS
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]