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The map label for this gene is nagP [H]

Identifier: 56965099

GI number: 56965099

Start: 3462615

End: 3464075

Strand: Direct

Name: nagP [H]

Synonym: ABC3337

Alternate gene names: 56965099

Gene position: 3462615-3464075 (Clockwise)

Preceding gene: 56965098

Following gene: 56965100

Centisome position: 80.45

GC content: 47.16

Gene sequence:

>1461_bases
ATGATGAATTACTTACAGCGCATTGGGCGCTCATTGATGTTGCCAGTTGCCGTGCTGCCTGCAGCTGCGATATTAATGGG
AATCGGCTATTGGATGGACCCGAGCGGTTGGGGAGAAGGCAATCCGGTCGCTGCCTTTTTAATTGCAGCAGGCGAATCTC
TTGTTAGCGGGCCGAGCATGGGCCTTTTGTTTGCAATCGGTGTAGCTCTAGGCATGGCAAAGGAGCGTGATGGATCAGCT
GCACTAAGCGGGCTCGTTGCTTTCCTCGTTGTAACCCACTTGTTGTCTACCGATTCAGTTGCACTGCTGACAAGCACGGA
CCCAGAAAATGTAAATGCCGCGTTTGGTTCGATTCAGACGCAATTTATTGGGATTTTATCCGGTATTGTCGCTGCTATGA
TGTATAACCGTTTCAGCCATGTCCAACTTCCGGCCGCATTTGCTTTTTTCAGCGGAAAACGGCTCGTGCCAATTATGACG
GCAGCTTCCATGTTGGTCATTTCCGCCGTTCTTTTTATCGTCTGGCCATTTGTTTACTCTGGCCTTGTCACATTTGGAAC
GTGGATTAGCAACTTGCACTATGTAGGCGCTGGTGTATATGGATTTTTTAACCGCCTTCTCATCCCAACTGGTCTTCACC
ATGCCTTAAATTCCGTCTTTTGGTTTGACGTTGCTGGCATCAATGACATTGCGAATTTCTGGTCTGGAAGCGGTGAAAAA
GGCATTACAGGCATGTACCAAGCTGGCTTTTTTCCAGTTATGATGTTTGGTCTTCCAGCCGCAGCGCTAGCAATGTACCA
TACGGCGAAAACGAAACGCAAAAAACAAACAGCGTCGTTGCTACTCGCTGCAGGATTTGCTTCGTTTTTCACAGGTGTGA
CCGAGCCATTTGAATTCGCGTTTATGTTTGTAGCACCTGTTCTTTATGTAATCCACGCGGTGCTTACCGGCTTATCGTTG
GCCATTGCCGCCTATTTTCAGTGGACAGCTGGATTTGGCTTTAGCGCCGGACTAATTGACTTTATTTTAAGCTCAAGGCT
GCCGCTAGCGAACCAGCCATGGATGCTGCTTGTGCAAGGGCTTGGCTTTGCTGTCGTTTATTACGCGCTATTCCGCTTTA
CGATTGTCAAATTTAATTTGATGACGCCTGGGCGTGAAGAGGAGAACGATGAAGAAAAAGTGCCAGAGGATACTGAAAAC
GCTCAAGACAAGAGCAAGTATACAGCAATGGCGGCCATGATCTATGATGGACTAGGCGGTGATCGTAATGTCGTCTCAAT
TGAGAACTGTGTCACCCGCTTACGTGTCGAAGTCAAAAATATGGCTCAAGTCGACCAAACAAAAATTAAACGTACCGGCA
TTCCTGGCATTAATGTCGTATCCAATCATAATATTCAAGTCATTATTGGCCCACATGTACAATTTGTCGCTGACGAAATC
GAGAAAATCCGCAGTCGTTAA

Upstream 100 bases:

>100_bases
CATTACGTTTCCATGCGCTACCTCCGCCATTCCTTATTGCCATCAGGATGTTGAAAAGACGGTTGCAACAAACACTACTT
TAAAAAAGGATGGATCGTTG

Downstream 100 bases:

>100_bases
AATCGACTCAGACTAAATATAGAACATGTTTTGCTCAAAGGAAGTTGTTGAGCAACAATTGCAAAGCATACACGAGACAA
ATGAACGTGTGCGCTTGAGC

Product: PTS system, N-acetylglucosamine-specific enzyme II, CB component

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MMNYLQRIGRSLMLPVAVLPAAAILMGIGYWMDPSGWGEGNPVAAFLIAAGESLVSGPSMGLLFAIGVALGMAKERDGSA
ALSGLVAFLVVTHLLSTDSVALLTSTDPENVNAAFGSIQTQFIGILSGIVAAMMYNRFSHVQLPAAFAFFSGKRLVPIMT
AASMLVISAVLFIVWPFVYSGLVTFGTWISNLHYVGAGVYGFFNRLLIPTGLHHALNSVFWFDVAGINDIANFWSGSGEK
GITGMYQAGFFPVMMFGLPAAALAMYHTAKTKRKKQTASLLLAAGFASFFTGVTEPFEFAFMFVAPVLYVIHAVLTGLSL
AIAAYFQWTAGFGFSAGLIDFILSSRLPLANQPWMLLVQGLGFAVVYYALFRFTIVKFNLMTPGREEENDEEKVPEDTEN
AQDKSKYTAMAAMIYDGLGGDRNVVSIENCVTRLRVEVKNMAQVDQTKIKRTGIPGINVVSNHNIQVIIGPHVQFVADEI
EKIRSR

Sequences:

>Translated_486_residues
MMNYLQRIGRSLMLPVAVLPAAAILMGIGYWMDPSGWGEGNPVAAFLIAAGESLVSGPSMGLLFAIGVALGMAKERDGSA
ALSGLVAFLVVTHLLSTDSVALLTSTDPENVNAAFGSIQTQFIGILSGIVAAMMYNRFSHVQLPAAFAFFSGKRLVPIMT
AASMLVISAVLFIVWPFVYSGLVTFGTWISNLHYVGAGVYGFFNRLLIPTGLHHALNSVFWFDVAGINDIANFWSGSGEK
GITGMYQAGFFPVMMFGLPAAALAMYHTAKTKRKKQTASLLLAAGFASFFTGVTEPFEFAFMFVAPVLYVIHAVLTGLSL
AIAAYFQWTAGFGFSAGLIDFILSSRLPLANQPWMLLVQGLGFAVVYYALFRFTIVKFNLMTPGREEENDEEKVPEDTEN
AQDKSKYTAMAAMIYDGLGGDRNVVSIENCVTRLRVEVKNMAQVDQTKIKRTGIPGINVVSNHNIQVIIGPHVQFVADEI
EKIRSR
>Mature_486_residues
MMNYLQRIGRSLMLPVAVLPAAAILMGIGYWMDPSGWGEGNPVAAFLIAAGESLVSGPSMGLLFAIGVALGMAKERDGSA
ALSGLVAFLVVTHLLSTDSVALLTSTDPENVNAAFGSIQTQFIGILSGIVAAMMYNRFSHVQLPAAFAFFSGKRLVPIMT
AASMLVISAVLFIVWPFVYSGLVTFGTWISNLHYVGAGVYGFFNRLLIPTGLHHALNSVFWFDVAGINDIANFWSGSGEK
GITGMYQAGFFPVMMFGLPAAALAMYHTAKTKRKKQTASLLLAAGFASFFTGVTEPFEFAFMFVAPVLYVIHAVLTGLSL
AIAAYFQWTAGFGFSAGLIDFILSSRLPLANQPWMLLVQGLGFAVVYYALFRFTIVKFNLMTPGREEENDEEKVPEDTEN
AQDKSKYTAMAAMIYDGLGGDRNVVSIENCVTRLRVEVKNMAQVDQTKIKRTGIPGINVVSNHNIQVIIGPHVQFVADEI
EKIRSR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=496, Percent_Identity=40.9274193548387, Blast_Score=318, Evalue=7e-88,
Organism=Escherichia coli, GI1787343, Length=502, Percent_Identity=37.0517928286853, Blast_Score=294, Evalue=8e-81,
Organism=Escherichia coli, GI1787908, Length=518, Percent_Identity=34.3629343629344, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 52647; Mature: 52647

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCC
>Mature Secondary Structure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HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]