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The map label for this gene is potI [H]

Identifier: 56964971

GI number: 56964971

Start: 3326068

End: 3326859

Strand: Direct

Name: potI [H]

Synonym: ABC3207

Alternate gene names: 56964971

Gene position: 3326068-3326859 (Clockwise)

Preceding gene: 56964970

Following gene: 56964972

Centisome position: 77.28

GC content: 44.44

Gene sequence:

>792_bases
ATGAACCAATCAAAACATATCGGCCTTGGGTTTGTTTCCTTTCTCATATTTTTGTTTTTGCTCGGGCCACTCGTTATTAT
TTCGGTTGCTTCTTTTGAGCCTAGTACAGTGCTGCAGTTTCCGCCAAGTGGCTTTTCCCTTGAATGGTACGCAAATATTT
TTGCTGTGTCGGCTTTCATGGACACGTTCAAGCTCTCAATGCTTGTTAGTTTGTTAGGCAACCTGTGTGCATTGTTGCTC
GGGATTCCTGCTGCCTATGCATTAAGCCGCTACGAGTTTCCAGGAAAAGCGCTCTTGAATGTGTTGTTCGTATCGCCGAT
ACTCATTCCTGGCACCGTGCTCGGTTTTACATTTTTACGCTATTTGATTGTCGCTTATCAGCTTCCGCTTGAAGTCGCGC
TATTTGTAGGGCATACGATTTTGATGCTGCCCTTTATTATCCGTGTGATTGCTTCTAGTATGGCCACGTTTGACTTCTCG
ATTGAAGAAGCAGCGATGAGCCTCGGGGCAAGCCGCTTTGGCGCGTTTTTCAAAGTCATTTTGCCTAATATTAAATCTGG
CATTATTGCGGCAGTGCTCATTGCGTTTCTAGAGTCATTCAACAACGTTGATATTTCCGTCTTTCTTACCGGGCCAGGGC
TCTCAACGCTGCCTATCCAAATGCTGACATACGTTGAACATTATTTTGACCCAACGGTTGCGGCTATTTCGGTGATGTTA
ATGGCATTAACAGCTATATTGATGTTTGCGATTGAAAGGCTAGTCGGCTTGTCGTATTTTACGAAACGATAA

Upstream 100 bases:

>100_bases
GCTGCTGCGATGATTGCAACGATTATGATTGTCATCACGTTTGTCGTGACCGGTCTTTTCCAAAAAATCGCAGCGATTTT
AAATCCAAGGGGGTAAACAG

Downstream 100 bases:

>100_bases
CGAAACCGATTCCCGTTTCTCCTATGCTTGATGGCAACTTTTGCAAAAGAATGGAGGTCTCTTATGGCTTTACTAACATT
AGACCAAGTGACGGTCGCTT

Product: spermidine/putrescine ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNQSKHIGLGFVSFLIFLFLLGPLVIISVASFEPSTVLQFPPSGFSLEWYANIFAVSAFMDTFKLSMLVSLLGNLCALLL
GIPAAYALSRYEFPGKALLNVLFVSPILIPGTVLGFTFLRYLIVAYQLPLEVALFVGHTILMLPFIIRVIASSMATFDFS
IEEAAMSLGASRFGAFFKVILPNIKSGIIAAVLIAFLESFNNVDISVFLTGPGLSTLPIQMLTYVEHYFDPTVAAISVML
MALTAILMFAIERLVGLSYFTKR

Sequences:

>Translated_263_residues
MNQSKHIGLGFVSFLIFLFLLGPLVIISVASFEPSTVLQFPPSGFSLEWYANIFAVSAFMDTFKLSMLVSLLGNLCALLL
GIPAAYALSRYEFPGKALLNVLFVSPILIPGTVLGFTFLRYLIVAYQLPLEVALFVGHTILMLPFIIRVIASSMATFDFS
IEEAAMSLGASRFGAFFKVILPNIKSGIIAAVLIAFLESFNNVDISVFLTGPGLSTLPIQMLTYVEHYFDPTVAAISVML
MALTAILMFAIERLVGLSYFTKR
>Mature_263_residues
MNQSKHIGLGFVSFLIFLFLLGPLVIISVASFEPSTVLQFPPSGFSLEWYANIFAVSAFMDTFKLSMLVSLLGNLCALLL
GIPAAYALSRYEFPGKALLNVLFVSPILIPGTVLGFTFLRYLIVAYQLPLEVALFVGHTILMLPFIIRVIASSMATFDFS
IEEAAMSLGASRFGAFFKVILPNIKSGIIAAVLIAFLESFNNVDISVFLTGPGLSTLPIQMLTYVEHYFDPTVAAISVML
MALTAILMFAIERLVGLSYFTKR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=240, Percent_Identity=28.3333333333333, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787368, Length=211, Percent_Identity=28.436018957346, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787714, Length=231, Percent_Identity=30.7359307359307, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788764, Length=216, Percent_Identity=26.3888888888889, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787571, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787080, Length=206, Percent_Identity=27.1844660194175, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28766; Mature: 28766

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00878 ODR_DC_2_1 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]