BacMap

Definition	Bacillus clausii KSM-K16, complete genome.
Accession	NC_006582
Length	4,303,871

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The map label for this gene is yqhE [C]

Identifier: 56963875

GI number: 56963875

Start: 2229729

End: 2230553

Strand: Direct

Name: yqhE [C]

Synonym: ABC2110

Alternate gene names: 56963875

Gene position: 2229729-2230553 (Clockwise)

Preceding gene: 56963872

Following gene: 56963876

Centisome position: 51.81

GC content: 46.18

Gene sequence:

>825_bases
ATGAAAATGGTGACATTAAACAACGGTGTAAACATGCCGCAGCTTGGTTTTGGTGTTTGGCAAGTGCCAGATGAAGAAGC
AACGCCCGCTGTTGCTAAAGCGCTCGAAGTGGGATATACGTCGATTGACACAGCGATGATTTACAAAAACGAAGCCGGCG
TTGGGAAAGCCATTAAAGAATCGGGCATTGATCGAGAAAAGCTATTTATTACAACGAAAGTGTGGAATGCAGACCAAGGG
TACGAGCAAACACTGAAAGCGTTTGATGCGAGCCTGGAGCGCCTTGGCCTTGACTACATCGACCTCTATTTGATTCATTG
GCCGACGCCTCATTATGACCAGTACATTGAGACATACAAAGCGCTTGAGAAATTGTACAACGATGGCCGCGTCCGGGCAA
TCGGCGTTTGTAACTTCGACATTGACCACTTGGAGCGACTCCTTGACGAGTGCGATATAACGCCTGTATTAAACCAAGTG
GAATGCCATCCATACTTTGCCCAAAATGAACTAAAAGACTTTTGTGCCAAACACAATATTTATGTGGAAGCATGGAGTCC
CCTTGACCAAGGCGGCGAGGTACTAACCGACGAAGTCGTTGAAAAAATTGCCAAAGCACATAACAAAACTACGGCACAAG
TCGTCCTCCGCTGGCACTTGCAAAATAATACAATTGTGATTCCTAAATCAGTCACGCCATCGCGGATTGAAGAAAACTTT
GATGTGTTCGATTTTGAACTGTCCGATGAAGAAATGGATGCGATTAATCGCCTTGACCGCAACCAACGCCGGGGCGCTAA
ACCGAGCGAAATGAATACGCGTTAA

Upstream 100 bases:

>100_bases
CAGTTGTCTTAACACAAGCAAGCACAAAAAAGGACAAGTATCGGTAAATGTCGTATACTATCATAGAGTTGATCCAATTC
CGATAAAGGAGTGTCTGAAC

Downstream 100 bases:

>100_bases
AATCCAACCAGCAAGCCCTTTCGGGTTTGCTGGTTGTTTCCTTTTTGACATTTCCAGTGATCGTAAAAAATAGGGATTTC
TATGATTTTCGTGAAAATCG

Product: oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKMVTLNNGVNMPQLGFGVWQVPDEEATPAVAKALEVGYTSIDTAMIYKNEAGVGKAIKESGIDREKLFITTKVWNADQG
YEQTLKAFDASLERLGLDYIDLYLIHWPTPHYDQYIETYKALEKLYNDGRVRAIGVCNFDIDHLERLLDECDITPVLNQV
ECHPYFAQNELKDFCAKHNIYVEAWSPLDQGGEVLTDEVVEKIAKAHNKTTAQVVLRWHLQNNTIVIPKSVTPSRIEENF
DVFDFELSDEEMDAINRLDRNQRRGAKPSEMNTR

Sequences:

>Translated_274_residues
MKMVTLNNGVNMPQLGFGVWQVPDEEATPAVAKALEVGYTSIDTAMIYKNEAGVGKAIKESGIDREKLFITTKVWNADQG
YEQTLKAFDASLERLGLDYIDLYLIHWPTPHYDQYIETYKALEKLYNDGRVRAIGVCNFDIDHLERLLDECDITPVLNQV
ECHPYFAQNELKDFCAKHNIYVEAWSPLDQGGEVLTDEVVEKIAKAHNKTTAQVVLRWHLQNNTIVIPKSVTPSRIEENF
DVFDFELSDEEMDAINRLDRNQRRGAKPSEMNTR
>Mature_274_residues
MKMVTLNNGVNMPQLGFGVWQVPDEEATPAVAKALEVGYTSIDTAMIYKNEAGVGKAIKESGIDREKLFITTKVWNADQG
YEQTLKAFDASLERLGLDYIDLYLIHWPTPHYDQYIETYKALEKLYNDGRVRAIGVCNFDIDHLERLLDECDITPVLNQV
ECHPYFAQNELKDFCAKHNIYVEAWSPLDQGGEVLTDEVVEKIAKAHNKTTAQVVLRWHLQNNTIVIPKSVTPSRIEENF
DVFDFELSDEEMDAINRLDRNQRRGAKPSEMNTR

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=294, Percent_Identity=40.8163265306122, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=41.0958904109589, Blast_Score=212, Evalue=2e-55,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=41.0958904109589, Blast_Score=212, Evalue=2e-55,
Organism=Homo sapiens, GI93277124, Length=310, Percent_Identity=36.4516129032258, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI310109922, Length=281, Percent_Identity=40.2135231316726, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI4502049, Length=294, Percent_Identity=41.4965986394558, Blast_Score=197, Evalue=6e-51,
Organism=Homo sapiens, GI291291012, Length=267, Percent_Identity=41.1985018726592, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI45446745, Length=299, Percent_Identity=38.4615384615385, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI4503285, Length=299, Percent_Identity=38.4615384615385, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI310109920, Length=299, Percent_Identity=38.1270903010033, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=38.1270903010033, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI5174695, Length=298, Percent_Identity=35.5704697986577, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=37.037037037037, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=37.037037037037, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=34.6570397111913, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=33.0935251798561, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=40.9090909090909, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI207028673, Length=113, Percent_Identity=43.3628318584071, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI310109926, Length=113, Percent_Identity=42.4778761061947, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI27436969, Length=207, Percent_Identity=28.9855072463768, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4504825, Length=207, Percent_Identity=28.9855072463768, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI87082198, Length=271, Percent_Identity=52.0295202952029, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1786400, Length=254, Percent_Identity=37.007874015748, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788081, Length=273, Percent_Identity=32.6007326007326, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788070, Length=315, Percent_Identity=25.3968253968254, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081735, Length=287, Percent_Identity=26.1324041811847, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994888, Length=234, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=39.3220338983051, Blast_Score=213, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17537075, Length=295, Percent_Identity=39.6610169491525, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17564128, Length=297, Percent_Identity=38.3838383838384, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=38.6029411764706, Blast_Score=200, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI71998625, Length=265, Percent_Identity=38.1132075471698, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=37.4570446735395, Blast_Score=188, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=37.3626373626374, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17537079, Length=293, Percent_Identity=32.7645051194539, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17566692, Length=293, Percent_Identity=33.4470989761092, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17562292, Length=276, Percent_Identity=34.7826086956522, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17552492, Length=261, Percent_Identity=30.6513409961686, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17561300, Length=274, Percent_Identity=33.5766423357664, Blast_Score=154, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17550246, Length=77, Percent_Identity=48.0519480519481, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324694, Length=299, Percent_Identity=40.8026755852843, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6320576, Length=284, Percent_Identity=36.9718309859155, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6319625, Length=290, Percent_Identity=41.3793103448276, Blast_Score=198, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6322556, Length=267, Percent_Identity=34.4569288389513, Blast_Score=175, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6321896, Length=308, Percent_Identity=33.1168831168831, Blast_Score=168, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6320079, Length=283, Percent_Identity=31.8021201413428, Blast_Score=138, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24663317, Length=290, Percent_Identity=41.7241379310345, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI20129731, Length=285, Percent_Identity=39.2982456140351, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24644950, Length=301, Percent_Identity=38.8704318936877, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI221378297, Length=321, Percent_Identity=37.0716510903427, Blast_Score=200, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24662789, Length=292, Percent_Identity=37.3287671232877, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI45553081, Length=289, Percent_Identity=36.6782006920415, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=39.0243902439024, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=35.8620689655172, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=40.5498281786942, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24662785, Length=293, Percent_Identity=39.9317406143345, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=35.546875, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31358; Mature: 31358

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMVTLNNGVNMPQLGFGVWQVPDEEATPAVAKALEVGYTSIDTAMIYKNEAGVGKAIKE
CEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHCEEEEEECCCCCCHHHHH
SGIDREKLFITTKVWNADQGYEQTLKAFDASLERLGLDYIDLYLIHWPTPHYDQYIETYK
CCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
ALEKLYNDGRVRAIGVCNFDIDHLERLLDECDITPVLNQVECHPYFAQNELKDFCAKHNI
HHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHCCE
YVEAWSPLDQGGEVLTDEVVEKIAKAHNKTTAQVVLRWHLQNNTIVIPKSVTPSRIEENF
EEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEECCCCHHHHHCCC
DVFDFELSDEEMDAINRLDRNQRRGAKPSEMNTR
CEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKMVTLNNGVNMPQLGFGVWQVPDEEATPAVAKALEVGYTSIDTAMIYKNEAGVGKAIKE
CEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHCEEEEEECCCCCCHHHHH
SGIDREKLFITTKVWNADQGYEQTLKAFDASLERLGLDYIDLYLIHWPTPHYDQYIETYK
CCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
ALEKLYNDGRVRAIGVCNFDIDHLERLLDECDITPVLNQVECHPYFAQNELKDFCAKHNI
HHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHCCE
YVEAWSPLDQGGEVLTDEVVEKIAKAHNKTTAQVVLRWHLQNNTIVIPKSVTPSRIEENF
EEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEECCCCHHHHHCCC
DVFDFELSDEEMDAINRLDRNQRRGAKPSEMNTR
CEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA