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The map label for this gene is exoA [H]

Identifier: 56963720

GI number: 56963720

Start: 2070851

End: 2071633

Strand: Direct

Name: exoA [H]

Synonym: ABC1955

Alternate gene names: 56963720

Gene position: 2070851-2071633 (Clockwise)

Preceding gene: 56963719

Following gene: 56963721

Centisome position: 48.12

GC content: 46.74

Gene sequence:

>783_bases
ATGAAATTCGTATCGTGGAATGTCAATGGCCTGCGCGCATGTGTAAAAAAAGGCTTTTTAGATTTTTTTCAACAAGTGGA
TGCGGACGTGTTTTGCTTACAAGAAACGAAGCTGCAAAAAGGGCAAATCTCGCTTGAGTTGCCTGGTTATGAACAATACT
GGAACGATGCGGAGCGTAAAGGCTATTCAGGGACAGCGATTTTTACTAAACACTCTCCGCTGTCTGTCACATATGGAATC
GGCGATCATTTTCCAGAGAACGAAGGGCGGGTCATCACCCTTGAGTTTGAGACCTACTATGTCGTAACGATGTATACCCC
TAATGCCAAACGAGACTTAAGCAGGCTGCCATACCGGCTGCAGTGGGAGAAAGCCGCTAGGGAATACCTGACTCTATTAG
CGACACAAAAACCAGTTGTCTTCTGCGGTGATCTCAATGTCGCCCACCAGCCGATTGATGTTCGAAACGACCGAGCGAAT
GTCGGCAATTCAGGTTTTACGGATGAAGAAAGAGCCGAGTTTGGCAAACTGCTGGAGGCAGGGTTTGTTGATACATTTCG
CCATTTTTACCCTGATGATGAGGATGCCTTTACATGGTGGTCGTACATGGCCAATGTCCGAGCCCGTAACATTGGCTGGC
GCATTGATTATTTCCTTATTTCCAGGGCGTTGATGCCTTATCTTGTCGACGCTTCTATTCATTCATCGGTTTTGGGCAGC
GATCACTGTCCGATAGAACTATGCCTCGATTTGCAACCAAATGAATCATCGCGACCGTTTTAA

Upstream 100 bases:

>100_bases
GCCAATACTCACGCCGAGGTACAGAAAGCAAAACGGTACTGGTTTTCTCTTATCTATGCTACACTGGGCACTGGATAAAG
AGAGAGGGAGTACGACAACT

Downstream 100 bases:

>100_bases
AGCGAGTTGCTCGTGCTCGTGCAACTCGCTTTTTCGGTCTTCCTATCGATAGTGTGTCATTGCTGACAATGAAGAACACT
GGGATAATTCGCCACTTCTG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKFVSWNVNGLRACVKKGFLDFFQQVDADVFCLQETKLQKGQISLELPGYEQYWNDAERKGYSGTAIFTKHSPLSVTYGI
GDHFPENEGRVITLEFETYYVVTMYTPNAKRDLSRLPYRLQWEKAAREYLTLLATQKPVVFCGDLNVAHQPIDVRNDRAN
VGNSGFTDEERAEFGKLLEAGFVDTFRHFYPDDEDAFTWWSYMANVRARNIGWRIDYFLISRALMPYLVDASIHSSVLGS
DHCPIELCLDLQPNESSRPF

Sequences:

>Translated_260_residues
MKFVSWNVNGLRACVKKGFLDFFQQVDADVFCLQETKLQKGQISLELPGYEQYWNDAERKGYSGTAIFTKHSPLSVTYGI
GDHFPENEGRVITLEFETYYVVTMYTPNAKRDLSRLPYRLQWEKAAREYLTLLATQKPVVFCGDLNVAHQPIDVRNDRAN
VGNSGFTDEERAEFGKLLEAGFVDTFRHFYPDDEDAFTWWSYMANVRARNIGWRIDYFLISRALMPYLVDASIHSSVLGS
DHCPIELCLDLQPNESSRPF
>Mature_260_residues
MKFVSWNVNGLRACVKKGFLDFFQQVDADVFCLQETKLQKGQISLELPGYEQYWNDAERKGYSGTAIFTKHSPLSVTYGI
GDHFPENEGRVITLEFETYYVVTMYTPNAKRDLSRLPYRLQWEKAAREYLTLLATQKPVVFCGDLNVAHQPIDVRNDRAN
VGNSGFTDEERAEFGKLLEAGFVDTFRHFYPDDEDAFTWWSYMANVRARNIGWRIDYFLISRALMPYLVDASIHSSVLGS
DHCPIELCLDLQPNESSRPF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=257, Percent_Identity=50.5836575875486, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI18375503, Length=257, Percent_Identity=50.5836575875486, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI18375501, Length=257, Percent_Identity=50.5836575875486, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI18375507, Length=314, Percent_Identity=29.2993630573248, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=32.8301886792453, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=40.3921568627451, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71989539, Length=144, Percent_Identity=35.4166666666667, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=49.6, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=48.8188976377953, Blast_Score=261, Evalue=3e-70,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29987; Mature: 29987

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVSWNVNGLRACVKKGFLDFFQQVDADVFCLQETKLQKGQISLELPGYEQYWNDAERK
CCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHCHHHHC
GYSGTAIFTKHSPLSVTYGIGDHFPENEGRVITLEFETYYVVTMYTPNAKRDLSRLPYRL
CCCCCEEEEECCCCEEEECCCCCCCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHCCEEE
QWEKAAREYLTLLATQKPVVFCGDLNVAHQPIDVRNDRANVGNSGFTDEERAEFGKLLEA
EHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GFVDTFRHFYPDDEDAFTWWSYMANVRARNIGWRIDYFLISRALMPYLVDASIHSSVLGS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCC
DHCPIELCLDLQPNESSRPF
CCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKFVSWNVNGLRACVKKGFLDFFQQVDADVFCLQETKLQKGQISLELPGYEQYWNDAERK
CCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHCHHHHC
GYSGTAIFTKHSPLSVTYGIGDHFPENEGRVITLEFETYYVVTMYTPNAKRDLSRLPYRL
CCCCCEEEEECCCCEEEECCCCCCCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHCCEEE
QWEKAAREYLTLLATQKPVVFCGDLNVAHQPIDVRNDRANVGNSGFTDEERAEFGKLLEA
EHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GFVDTFRHFYPDDEDAFTWWSYMANVRARNIGWRIDYFLISRALMPYLVDASIHSSVLGS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCC
DHCPIELCLDLQPNESSRPF
CCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]