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The map label for this gene is mccA [H]

Identifier: 56963709

GI number: 56963709

Start: 2059135

End: 2060061

Strand: Direct

Name: mccA [H]

Synonym: ABC1944

Alternate gene names: 56963709

Gene position: 2059135-2060061 (Clockwise)

Preceding gene: 56963708

Following gene: 56963710

Centisome position: 47.84

GC content: 49.3

Gene sequence:

>927_bases
ATGGAAGTGTACCGCCATATCCATGAACTCATAGGTTCAACCCCCATGCTTGAACTGACGGCCTTTCCGCTCCCGCGCCG
TGTGCGTTTATTTGCAAAGTTGGAGTATACCAATCCTGGAGGAAGCATTAAAGACCGTTTAGGCATCAAACTCCTCAAGG
AAGCAATTGCCACCGGTAAAATCACACCGAAGGGCACATTGATTGAGCCAACGGCTGGAAATACAGGGATTGCTCTTGCG
CTCGCAGCGATTGGCACGGATGTAAAGGTCATTTGTGTCGTGCCAAAGGCATTTAGCATCGAAAAGCAGCAGCTGATGCG
CGCCCTTGGAGCTGTCATTGTCCATACACCGAGTGAACATGGGATGAAGGGAGCAATTGCAAAAGCAAAAGCGTTAGAAC
AAGAGCTTGATGATGCTTATTGCCCGCAGCAGTTCAGCAATAAAGCGAATCCACTCGCTTATTATGAGACGCTTGGCCCA
GAAATCGTTCACGAGCTTGATGGCAAAGTTGATGTTTTTGTTGCAGGCGCTGGAACAGGAGGGACATTTTCAGGAACAGC
CGCTTATTTAAAAGAAGTGATCCCTGGCATTCGCACTGTTGTGGTGGAACCAGAGGGTTCGATTTTAAATGGTGGGGAGC
CAGGACCGCACCGGACTGAGGGAATTGGGATGGAATTCATCCCAGACTTTGTAAACAGGTCTTTATTTGAAGCCATTTAT
ACGATTTCAGACAACCAAGCGTTTAAGCGGACACGCGAACTCGCTGAACATCAAGGTGTGCTTGTCGGCAGTTCTTCAGG
TGCTGCTCTTGAAGCTGCCTTGCGAGAGGCTGAAATGGCCGACGAAGGTGCCCATATCGTCACTGTCTTTCCTGACGGGG
CGGAACGGTATTTGAGCAAAGGCATTTATGAGGAGGAACCTTTGTGA

Upstream 100 bases:

>100_bases
CATGTGTCCTGGCGGCGCTTTTTCTCTTCTTCAAAAGTAGGCACCAATCCAAATTCGCGACATACGTTGAAATAAACGGC
ATTGCAAATGAGGTGGTTAG

Downstream 100 bases:

>100_bases
GGAAAAAGACACAGCTTATCCATGGTGGCATTGGAATGGATGCTGAAACAGGCGCAGTCACGCCACCGATCCATCCAGCC
AGCACATTTAAACAAAAAGG

Product: cysteine synthase

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEVYRHIHELIGSTPMLELTAFPLPRRVRLFAKLEYTNPGGSIKDRLGIKLLKEAIATGKITPKGTLIEPTAGNTGIALA
LAAIGTDVKVICVVPKAFSIEKQQLMRALGAVIVHTPSEHGMKGAIAKAKALEQELDDAYCPQQFSNKANPLAYYETLGP
EIVHELDGKVDVFVAGAGTGGTFSGTAAYLKEVIPGIRTVVVEPEGSILNGGEPGPHRTEGIGMEFIPDFVNRSLFEAIY
TISDNQAFKRTRELAEHQGVLVGSSSGAALEAALREAEMADEGAHIVTVFPDGAERYLSKGIYEEEPL

Sequences:

>Translated_308_residues
MEVYRHIHELIGSTPMLELTAFPLPRRVRLFAKLEYTNPGGSIKDRLGIKLLKEAIATGKITPKGTLIEPTAGNTGIALA
LAAIGTDVKVICVVPKAFSIEKQQLMRALGAVIVHTPSEHGMKGAIAKAKALEQELDDAYCPQQFSNKANPLAYYETLGP
EIVHELDGKVDVFVAGAGTGGTFSGTAAYLKEVIPGIRTVVVEPEGSILNGGEPGPHRTEGIGMEFIPDFVNRSLFEAIY
TISDNQAFKRTRELAEHQGVLVGSSSGAALEAALREAEMADEGAHIVTVFPDGAERYLSKGIYEEEPL
>Mature_308_residues
MEVYRHIHELIGSTPMLELTAFPLPRRVRLFAKLEYTNPGGSIKDRLGIKLLKEAIATGKITPKGTLIEPTAGNTGIALA
LAAIGTDVKVICVVPKAFSIEKQQLMRALGAVIVHTPSEHGMKGAIAKAKALEQELDDAYCPQQFSNKANPLAYYETLGP
EIVHELDGKVDVFVAGAGTGGTFSGTAAYLKEVIPGIRTVVVEPEGSILNGGEPGPHRTEGIGMEFIPDFVNRSLFEAIY
TISDNQAFKRTRELAEHQGVLVGSSSGAALEAALREAEMADEGAHIVTVFPDGAERYLSKGIYEEEPL

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=301, Percent_Identity=38.8704318936877, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI295821200, Length=301, Percent_Identity=38.8704318936877, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4557415, Length=301, Percent_Identity=38.8704318936877, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1788754, Length=308, Percent_Identity=41.2337662337662, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI2367138, Length=302, Percent_Identity=36.4238410596026, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=40, Blast_Score=211, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=40.3333333333333, Blast_Score=207, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=41.296928327645, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115535073, Length=303, Percent_Identity=42.2442244224422, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=41.8060200668896, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25147552, Length=306, Percent_Identity=38.2352941176471, Blast_Score=173, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17534315, Length=319, Percent_Identity=37.6175548589342, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=40.1869158878505, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=39.3162393162393, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=323, Percent_Identity=40.2476780185758, Blast_Score=208, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321449, Length=336, Percent_Identity=32.1428571428571, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24643623, Length=315, Percent_Identity=37.4603174603175, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI20129101, Length=315, Percent_Identity=37.4603174603175, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 33022; Mature: 33022

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVYRHIHELIGSTPMLELTAFPLPRRVRLFAKLEYTNPGGSIKDRLGIKLLKEAIATGK
CHHHHHHHHHHCCCCEEEEECCCCCHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
ITPKGTLIEPTAGNTGIALALAAIGTDVKVICVVPKAFSIEKQQLMRALGAVIVHTPSEH
CCCCCCEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCEEEECCCCC
GMKGAIAKAKALEQELDDAYCPQQFSNKANPLAYYETLGPEIVHELDGKVDVFVAGAGTG
CCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHCCHHHHHHCCCCEEEEEEECCCC
GTFSGTAAYLKEVIPGIRTVVVEPEGSILNGGEPGPHRTEGIGMEFIPDFVNRSLFEAIY
CCCCHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TISDNQAFKRTRELAEHQGVLVGSSSGAALEAALREAEMADEGAHIVTVFPDGAERYLSK
HCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHC
GIYEEEPL
CCCCCCCC
>Mature Secondary Structure
MEVYRHIHELIGSTPMLELTAFPLPRRVRLFAKLEYTNPGGSIKDRLGIKLLKEAIATGK
CHHHHHHHHHHCCCCEEEEECCCCCHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
ITPKGTLIEPTAGNTGIALALAAIGTDVKVICVVPKAFSIEKQQLMRALGAVIVHTPSEH
CCCCCCEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCEEEECCCCC
GMKGAIAKAKALEQELDDAYCPQQFSNKANPLAYYETLGPEIVHELDGKVDVFVAGAGTG
CCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHCCHHHHHHCCCCEEEEEEECCCC
GTFSGTAAYLKEVIPGIRTVVVEPEGSILNGGEPGPHRTEGIGMEFIPDFVNRSLFEAIY
CCCCHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TISDNQAFKRTRELAEHQGVLVGSSSGAALEAALREAEMADEGAHIVTVFPDGAERYLSK
HCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHC
GIYEEEPL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 3.6 {S2-}} 0.1 {S2-}} 0.12 {acetyl-CoA}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]