Click here to switch to the map view.

The map label for this gene is sigF [H]

Identifier: 56963560

GI number: 56963560

Start: 1912149

End: 1912913

Strand: Direct

Name: sigF [H]

Synonym: ABC1795

Alternate gene names: 56963560

Gene position: 1912149-1912913 (Clockwise)

Preceding gene: 56963559

Following gene: 56963561

Centisome position: 44.43

GC content: 44.31

Gene sequence:

>765_bases
ATGAGTGCAGAGGTGAAAAACGGCGGCAAAAAAAAGCCACTATCCGATAAACAAGTGAAAGAGCTGATTGCAAAAAGCCA
GGAAGGCGACACAGAAGCACGGGATTCGATCGTCAACCATAACACACGTCTCGTCTGGTCAGTCGTCCAACGTTTTTTGA
ATCGCGGTTATGAGGCAGACGACCTTTTTCAAATTGGCTGCATTGGTTTGATTAAGTCTGTCGACAAATTTGACCTTTCC
TATGATGTGAAATTCTCCACGTATGCTGTGCCAATGATTATTGGGGAAATCCAACGTTTTTTGCGGGATGATGGTACGGT
GAAAGTAAGCCGGTCCATTAAAGAATTAAGCAATAAAATTCGCAAAGCAAAAGACGAGCTGACGAAAACGCTGCGCCGGG
CGCCGACCATTAATGAGATTGCCGAACATTTAGGCGTGACGCCTGAGGAAATTGTATTTGCTGGAGATGCCAACCGGAGC
TTGTCTTCAATCCATGAAACGGTCTATGAAAATGATGGCGACCCGATTACACTGCTAGATCAAATTGCCGACCACTCACA
AGTCAAGTGGTTTGACAAGATTGCTTTAAAAGAAGCCATTTGCGACCTTGGCGAAAGGGAGCGGCTGATAGTATACTTGC
GTTATTATAAGGATCAAACACAATCAGAAGTGGCTGAACGCCTAGGCATTTCGCAAGTGCAAGTTTCTCGGCTAGAGAAA
AAAATATTGGAACAAATGAAAGAAACAATGTCAGACGCCACTTAA

Upstream 100 bases:

>100_bases
AGAGTTGAAAGTAATCTCCGAACCAATGATTGGGACAACCGTTTACGTGAAAAAACAGCTGACCACGACGAAAGCAATGT
GCAGATAAGGGGTCTTGCTT

Downstream 100 bases:

>100_bases
AAAAGCAAGCTCAGAAGTTTAGGAGCTTGCTTTCTTTTTTGTCTAGGGGCCTATGTAGAGGAATGTGTAAGTATGGTACA
GACAATCCAGGTGGCAAGCT

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSAEVKNGGKKKPLSDKQVKELIAKSQEGDTEARDSIVNHNTRLVWSVVQRFLNRGYEADDLFQIGCIGLIKSVDKFDLS
YDVKFSTYAVPMIIGEIQRFLRDDGTVKVSRSIKELSNKIRKAKDELTKTLRRAPTINEIAEHLGVTPEEIVFAGDANRS
LSSIHETVYENDGDPITLLDQIADHSQVKWFDKIALKEAICDLGERERLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILEQMKETMSDAT

Sequences:

>Translated_254_residues
MSAEVKNGGKKKPLSDKQVKELIAKSQEGDTEARDSIVNHNTRLVWSVVQRFLNRGYEADDLFQIGCIGLIKSVDKFDLS
YDVKFSTYAVPMIIGEIQRFLRDDGTVKVSRSIKELSNKIRKAKDELTKTLRRAPTINEIAEHLGVTPEEIVFAGDANRS
LSSIHETVYENDGDPITLLDQIADHSQVKWFDKIALKEAICDLGERERLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
KILEQMKETMSDAT
>Mature_253_residues
SAEVKNGGKKKPLSDKQVKELIAKSQEGDTEARDSIVNHNTRLVWSVVQRFLNRGYEADDLFQIGCIGLIKSVDKFDLSY
DVKFSTYAVPMIIGEIQRFLRDDGTVKVSRSIKELSNKIRKAKDELTKTLRRAPTINEIAEHLGVTPEEIVFAGDANRSL
SSIHETVYENDGDPITLLDQIADHSQVKWFDKIALKEAICDLGERERLIVYLRYYKDQTQSEVAERLGISQVQVSRLEKK
ILEQMKETMSDAT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=248, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789098, Length=247, Percent_Identity=27.9352226720648, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788231, Length=198, Percent_Identity=29.2929292929293, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=27.1604938271605, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28894; Mature: 28763

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEVKNGGKKKPLSDKQVKELIAKSQEGDTEARDSIVNHNTRLVWSVVQRFLNRGYEAD
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
DLFQIGCIGLIKSVDKFDLSYDVKFSTYAVPMIIGEIQRFLRDDGTVKVSRSIKELSNKI
HHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RKAKDELTKTLRRAPTINEIAEHLGVTPEEIVFAGDANRSLSSIHETVYENDGDPITLLD
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHCCCCCCHHHH
QIADHSQVKWFDKIALKEAICDLGERERLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
HHCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILEQMKETMSDAT
HHHHHHHHHHCCCC
>Mature Secondary Structure 
SAEVKNGGKKKPLSDKQVKELIAKSQEGDTEARDSIVNHNTRLVWSVVQRFLNRGYEAD
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
DLFQIGCIGLIKSVDKFDLSYDVKFSTYAVPMIIGEIQRFLRDDGTVKVSRSIKELSNKI
HHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RKAKDELTKTLRRAPTINEIAEHLGVTPEEIVFAGDANRSLSSIHETVYENDGDPITLLD
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHCCCCCCHHHH
QIADHSQVKWFDKIALKEAICDLGERERLIVYLRYYKDQTQSEVAERLGISQVQVSRLEK
HHCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCHHHHHHHHHCHHHHHHHHHHH
KILEQMKETMSDAT
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]