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The map label for this gene is lepA

Identifier: 56963420

GI number: 56963420

Start: 1783174

End: 1784997

Strand: Direct

Name: lepA

Synonym: ABC1655

Alternate gene names: 56963420

Gene position: 1783174-1784997 (Clockwise)

Preceding gene: 56963419

Following gene: 56963421

Centisome position: 41.43

GC content: 45.34

Gene sequence:

>1824_bases
ATGAACAACGACCAACGACTTGAGCGGCAGTCGAGAATCCGAAACTTCTCGATTATCGCCCATATTGACCACGGGAAATC
AACATTGGCAGACCGCATCCTTGAAAGGACCGGGGCATTGACTGACCGTGAAATGAAAGAACAAGCGCTTGATGCAATGG
ACTTGGAACGCGAGCGAGGCATAACGATTAAGCTGAACGCAGTCCAATTGAAATACAAAGCCAAAGACGGAAACGAGTAT
ATTTTTCATTTAATTGATACGCCAGGACATGTCGATTTTACTTATGAAGTGTCCCGCAGCCTCGCTGCTTGTGAAGGGGC
GCTTCTTATCGTTGATAGCGCCCAAGGAATTGAGGCGCAAACGCTCGCCAACGTTTACTTGGCATTAGACAATGACTTGG
AAATTTTGCCTGTTATTAATAAAATCGACTTGCCAAGCGCAGAGCCAGAACGTGTGAAAAAAGAAGTGGAAGACGTCATT
GGCCTTGATGCTTCCGATGCTGTATTGGCTTCTGCCAAAAATGGCATCGGCATTGAAGACATTTTGGAACAGGTCGTTGA
GAAAGTGCCTGCCCCTGCTGGCGATCCAACAGCACCGCTTAAAGCGTTAATATTTGACTCGCTCTATGATCCATACCGTG
GCGTCATTGCTTATATCCGTATCGTCGAGGGCACTGTTCGCCCTGGTGAAAAAATTAGGATGATGCAAACAGGGAAGGAA
TTTGAAGTGAATGAAGTTGGCGTATTTACGCCAAAAGCAGTCAAATGCGAAGAATTAACTGTAGGTGATGTAGGCTTTTT
AACAGCAGCGATCAAAAATGTCAGCGACTCTCGTGTCGGCGACACGATAACAAGCGCCGTCAATCCAGCACAAGAGGCAT
TGCCTGGCTATCGGCGGATGAATCCAATGGTCTATTGCGGCCTTTATCCGATCGATACAAATGATTACAACGATCTACGC
GAGGCATTGGAGCGCCTCGAGCTGAATGATGCTTCTCTCCAGTATGAACCAGAAACGTCACAAGCACTTGGTTTTGGTTT
CCGCTGCGGCTTTTTAGGATTGCTTCACATGGAAATTATTCAGGAGCGGATTGAACGGGAATTTGGGATTTCCTTAATTA
CGACAGCGCCATCGGTTATTTACCATGTGACGCTTACAGACGGCAATACGATCCAAATTGACAACCCGACAAATATGCCT
GATCCCCAAAAAATGGACCATGTCGAAGAGCCGTATGTCAAATCGACTGTGATGGTGCCTAATGACTATGTTGGTGCAGT
CATGGAGCTTTGCCAAAGCAAGCGCGGCATTTTTGTGGACATGCAGTATTTGGATGAAAACCGCGTACAAATCATTTATG
AGATCCCTTTATCGGAAATTGTCTACGATTTCTTTGATCAATTGAAATCGAATACGAAAGGGTATGCCTCTTTTGATTAT
GAACTAATCGGCTACAGGCAATCAAAATTAGTAAAAATGGACATTTTATTGAACGGCGAGAAAGTTGACGCTCTGTCCGT
CATTGTCCACCGCGATTCGGCATATGAACGGGGGAAAGTGATCGTCGAAAAACTGAAAGAGTTAATTCCGCGCCAGCAAT
TTGAAGTGCCTGTCCAAGCAAGTATTGGCACAAAAATTATCGCTCGTTCGACGATCAAAGCAATCCGCAAAAATGTGTTA
GCTAAATGTTATGGCGGCGACATTTCGCGTAAACGAAAGCTTCTTGAGAAGCAAAAAGAAGGCAAGAAGCGAATGAAAGC
AGTTGGAAACGTCGAGATTCCACAGGAAGCTTTTATGGCTGTACTGCGCATGGACGACAAGTAA

Upstream 100 bases:

>100_bases
CATGACGAATCCGCTGAAAGCTCTTATTGAAGCGCCGCCATTGGCTTGCTATAATAAGGGGCAGTGTACATGTACGCATT
TTAGGGGTGAGTCAAGAAAA

Downstream 100 bases:

>100_bases
GCAATACTTGTGCATGATGCCCTTATAGAATTGTCCGCTTCCAGCCGTAAAAGAGCCGACCTCTAAATGAAAATACGTAG
AGGCCACCTTCTGTATTTAG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MNNDQRLERQSRIRNFSIIAHIDHGKSTLADRILERTGALTDREMKEQALDAMDLERERGITIKLNAVQLKYKAKDGNEY
IFHLIDTPGHVDFTYEVSRSLAACEGALLIVDSAQGIEAQTLANVYLALDNDLEILPVINKIDLPSAEPERVKKEVEDVI
GLDASDAVLASAKNGIGIEDILEQVVEKVPAPAGDPTAPLKALIFDSLYDPYRGVIAYIRIVEGTVRPGEKIRMMQTGKE
FEVNEVGVFTPKAVKCEELTVGDVGFLTAAIKNVSDSRVGDTITSAVNPAQEALPGYRRMNPMVYCGLYPIDTNDYNDLR
EALERLELNDASLQYEPETSQALGFGFRCGFLGLLHMEIIQERIEREFGISLITTAPSVIYHVTLTDGNTIQIDNPTNMP
DPQKMDHVEEPYVKSTVMVPNDYVGAVMELCQSKRGIFVDMQYLDENRVQIIYEIPLSEIVYDFFDQLKSNTKGYASFDY
ELIGYRQSKLVKMDILLNGEKVDALSVIVHRDSAYERGKVIVEKLKELIPRQQFEVPVQASIGTKIIARSTIKAIRKNVL
AKCYGGDISRKRKLLEKQKEGKKRMKAVGNVEIPQEAFMAVLRMDDK

Sequences:

>Translated_607_residues
MNNDQRLERQSRIRNFSIIAHIDHGKSTLADRILERTGALTDREMKEQALDAMDLERERGITIKLNAVQLKYKAKDGNEY
IFHLIDTPGHVDFTYEVSRSLAACEGALLIVDSAQGIEAQTLANVYLALDNDLEILPVINKIDLPSAEPERVKKEVEDVI
GLDASDAVLASAKNGIGIEDILEQVVEKVPAPAGDPTAPLKALIFDSLYDPYRGVIAYIRIVEGTVRPGEKIRMMQTGKE
FEVNEVGVFTPKAVKCEELTVGDVGFLTAAIKNVSDSRVGDTITSAVNPAQEALPGYRRMNPMVYCGLYPIDTNDYNDLR
EALERLELNDASLQYEPETSQALGFGFRCGFLGLLHMEIIQERIEREFGISLITTAPSVIYHVTLTDGNTIQIDNPTNMP
DPQKMDHVEEPYVKSTVMVPNDYVGAVMELCQSKRGIFVDMQYLDENRVQIIYEIPLSEIVYDFFDQLKSNTKGYASFDY
ELIGYRQSKLVKMDILLNGEKVDALSVIVHRDSAYERGKVIVEKLKELIPRQQFEVPVQASIGTKIIARSTIKAIRKNVL
AKCYGGDISRKRKLLEKQKEGKKRMKAVGNVEIPQEAFMAVLRMDDK
>Mature_607_residues
MNNDQRLERQSRIRNFSIIAHIDHGKSTLADRILERTGALTDREMKEQALDAMDLERERGITIKLNAVQLKYKAKDGNEY
IFHLIDTPGHVDFTYEVSRSLAACEGALLIVDSAQGIEAQTLANVYLALDNDLEILPVINKIDLPSAEPERVKKEVEDVI
GLDASDAVLASAKNGIGIEDILEQVVEKVPAPAGDPTAPLKALIFDSLYDPYRGVIAYIRIVEGTVRPGEKIRMMQTGKE
FEVNEVGVFTPKAVKCEELTVGDVGFLTAAIKNVSDSRVGDTITSAVNPAQEALPGYRRMNPMVYCGLYPIDTNDYNDLR
EALERLELNDASLQYEPETSQALGFGFRCGFLGLLHMEIIQERIEREFGISLITTAPSVIYHVTLTDGNTIQIDNPTNMP
DPQKMDHVEEPYVKSTVMVPNDYVGAVMELCQSKRGIFVDMQYLDENRVQIIYEIPLSEIVYDFFDQLKSNTKGYASFDY
ELIGYRQSKLVKMDILLNGEKVDALSVIVHRDSAYERGKVIVEKLKELIPRQQFEVPVQASIGTKIIARSTIKAIRKNVL
AKCYGGDISRKRKLLEKQKEGKKRMKAVGNVEIPQEAFMAVLRMDDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=599, Percent_Identity=49.7495826377296, Blast_Score=637, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=41.7910447761194, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=36.9127516778523, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI18390331, Length=181, Percent_Identity=35.3591160220994, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI19923640, Length=136, Percent_Identity=43.3823529411765, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI25306287, Length=136, Percent_Identity=43.3823529411765, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI25306283, Length=136, Percent_Identity=43.3823529411765, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=39.4495412844037, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=39.4495412844037, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=39.4495412844037, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272894, Length=140, Percent_Identity=33.5714285714286, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI217272892, Length=140, Percent_Identity=33.5714285714286, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI53729339, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI53729337, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=59.2905405405405, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=30.8910891089109, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=31.4465408805031, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789738, Length=184, Percent_Identity=33.1521739130435, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789559, Length=255, Percent_Identity=27.843137254902, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=42.7168576104746, Blast_Score=523, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17556745, Length=469, Percent_Identity=24.0938166311301, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=181, Percent_Identity=37.0165745856354, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=34.1614906832298, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=171, Percent_Identity=32.1637426900585, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=36.8421052631579, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=31.5789473684211, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=599, Percent_Identity=45.7429048414023, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=40.2684563758389, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=40.2684563758389, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=185, Percent_Identity=36.2162162162162, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=41.2280701754386, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=143, Percent_Identity=37.7622377622378, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=241, Percent_Identity=25.7261410788382, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78706572, Length=600, Percent_Identity=44, Blast_Score=532, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24582462, Length=186, Percent_Identity=37.6344086021505, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28574573, Length=221, Percent_Identity=34.841628959276, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=39.7350993377483, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=161, Percent_Identity=32.2981366459627, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28572034, Length=228, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921738, Length=231, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363316, Length=294, Percent_Identity=25.8503401360544, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864358, Length=294, Percent_Identity=25.8503401360544, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_BACSK (Q5WHG5)

Other databases:

- EMBL:   AP006627
- RefSeq:   YP_175151.1
- ProteinModelPortal:   Q5WHG5
- SMR:   Q5WHG5
- STRING:   Q5WHG5
- EnsemblBacteria:   EBBACT00000046342
- GeneID:   3204055
- GenomeReviews:   AP006627_GR
- KEGG:   bcl:ABC1655
- NMPDR:   fig|66692.3.peg.38
- eggNOG:   COG0481
- GeneTree:   EBGT00070000031741
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   BCLA66692:ABC1655-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 68074; Mature: 68074

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDQRLERQSRIRNFSIIAHIDHGKSTLADRILERTGALTDREMKEQALDAMDLERERG
CCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ITIKLNAVQLKYKAKDGNEYIFHLIDTPGHVDFTYEVSRSLAACEGALLIVDSAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHH
TLANVYLALDNDLEILPVINKIDLPSAEPERVKKEVEDVIGLDASDAVLASAKNGIGIED
HHEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHH
ILEQVVEKVPAPAGDPTAPLKALIFDSLYDPYRGVIAYIRIVEGTVRPGEKIRMMQTGKE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHEEHHHCCCC
FEVNEVGVFTPKAVKCEELTVGDVGFLTAAIKNVSDSRVGDTITSAVNPAQEALPGYRRM
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCCCHHHC
NPMVYCGLYPIDTNDYNDLREALERLELNDASLQYEPETSQALGFGFRCGFLGLLHMEII
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHH
QERIEREFGISLITTAPSVIYHVTLTDGNTIQIDNPTNMPDPQKMDHVEEPYVKSTVMVP
HHHHHHHHCCEEEECCCEEEEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCCCEEEECC
NDYVGAVMELCQSKRGIFVDMQYLDENRVQIIYEIPLSEIVYDFFDQLKSNTKGYASFDY
CHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEECE
ELIGYRQSKLVKMDILLNGEKVDALSVIVHRDSAYERGKVIVEKLKELIPRQQFEVPVQA
EEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHCCCCEEC
SIGTKIIARSTIKAIRKNVLAKCYGGDISRKRKLLEKQKEGKKRMKAVGNVEIPQEAFMA
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VLRMDDK
HHHCCCC
>Mature Secondary Structure
MNNDQRLERQSRIRNFSIIAHIDHGKSTLADRILERTGALTDREMKEQALDAMDLERERG
CCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ITIKLNAVQLKYKAKDGNEYIFHLIDTPGHVDFTYEVSRSLAACEGALLIVDSAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHH
TLANVYLALDNDLEILPVINKIDLPSAEPERVKKEVEDVIGLDASDAVLASAKNGIGIED
HHEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHH
ILEQVVEKVPAPAGDPTAPLKALIFDSLYDPYRGVIAYIRIVEGTVRPGEKIRMMQTGKE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHEEHHHCCCC
FEVNEVGVFTPKAVKCEELTVGDVGFLTAAIKNVSDSRVGDTITSAVNPAQEALPGYRRM
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCCCHHHC
NPMVYCGLYPIDTNDYNDLREALERLELNDASLQYEPETSQALGFGFRCGFLGLLHMEII
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHH
QERIEREFGISLITTAPSVIYHVTLTDGNTIQIDNPTNMPDPQKMDHVEEPYVKSTVMVP
HHHHHHHHCCEEEECCCEEEEEEEECCCCEEEECCCCCCCCCHHHCCCCCCCCCEEEECC
NDYVGAVMELCQSKRGIFVDMQYLDENRVQIIYEIPLSEIVYDFFDQLKSNTKGYASFDY
CHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEECE
ELIGYRQSKLVKMDILLNGEKVDALSVIVHRDSAYERGKVIVEKLKELIPRQQFEVPVQA
EEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHCCCCEEC
SIGTKIIARSTIKAIRKNVLAKCYGGDISRKRKLLEKQKEGKKRMKAVGNVEIPQEAFMA
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VLRMDDK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA