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The map label for this gene is narK [H]

Identifier: 56963390

GI number: 56963390

Start: 1755664

End: 1756869

Strand: Direct

Name: narK [H]

Synonym: ABC1625

Alternate gene names: 56963390

Gene position: 1755664-1756869 (Clockwise)

Preceding gene: 56963389

Following gene: 56963401

Centisome position: 40.79

GC content: 48.51

Gene sequence:

>1206_bases
ATGAAACTTTCTGAGTTACGCAAAAGCGGGCATGCGCCTTCGTTGTTTTCCGCTTTTTTATATTTCGATGTAAGCTTTAT
GATTTGGGTATTGCTTGGTGCCCTTGGCGCATACATTACTGCTGATTTTGGCTTGAGTGCTTCACAAATTGGGTTCATTG
TCGGGATTCCGATTTTAGCAGGCTCTGTTTTCCGCATCCTTGTTGGCATTTTGACAGATCGTTATGGGCCAAAAAAGACG
AGCATAGCAGGCATGGTTGTAACGATGGTACCGCTGTTGTGGGGCTGGCTATTTGGTACGAGCTTGTCAGAATTGATCGC
GATCGGGCTGCTGCTTGGCGTTGCGGGTGCTAGTTTTTCTGCTTCGCTGCCAATGGCTAGCCGGTGGTACCCCCCTCATT
TACAAGGAGTAGCAATGGGCATAGCAGGAGCCGGAAATAGTGGTACCTTATTTGCTACATTGTTCGGGCCAAGGCTGGCA
GAAATTGTTGGTTGGAATGGGGTGATGGGACTAGCGCTTATTCCTCTTTCCCTTGTGCTCATCTCTTACATCCTGATGGC
GAAGGAGCCACCAAACCAGCCAGCTCCAAAGCCGTTAAAAGCGTATTTCCGTGTATTCCAAGAGCGTGATACATGGTACT
TTTGCTTGCTTTACAGTGTTACATTTGGTGGATTTGTAGGCTTGTCAAGCTTTTTAAGCTATTTCTTTGTAAACCAGTAT
GCTGTTTCAGGGGTCAGAGCAGGGGAGTTTGTCACACTTGTCGTTGCTGCCGGCAGCTTATTGCGCCCAATTGGCGGGTT
GATTGCAGACAAAATTGGCGGTGCTCGCTTGCTTACTGTGCTTTTTGTTGGCGTAGCCCTTTGTATGTTTGCCGTTAGTT
TTCTCCCGCCGATTGCGTTTGCTCTTACTGTGTTATTTATAGGCATGGGCTGCCTCGGCATGGGCAACGGTGCTGTGTTT
CAACTTGTCCCGCAGCGTTTCCATAAAGAAATCGGCATGGTAACTGGCATCGTTGGAGCTGCTGGAGGCGTTGGCGGCTT
TTTCTTGCCAAATATTCTCGGCTTGTTTAAGGATATAACGGGGACGTACGCTAGCGGATTTCTTCTGTTCGCCCTTTCTG
CCTGTTTCGCCCTTGGGCTGTTACTGTTTGCTCAGCACTCATGGAAGAAGGCGTGGACAGAGACAAAGCAGACGACGTCT
ATTTAA

Upstream 100 bases:

>100_bases
GCAATTTAGACCCTGATTGCCGCATGCCAGCTTTTAAAGTCAGCGCAGTAAGGGCTGAACCATTAGTTGTTCATGAGCAA
ATGGAAGGAGTGTTGGGAAC

Downstream 100 bases:

>100_bases
GCGACTTGAAGATAAAATGGAGGCCAGCGAACGCTGGCCTCTGTTTTATTGGTGACGGTTTGTCTGTATCGATTGTTAAT
CGTTCTTTTTTGGTGGTTTG

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKLSELRKSGHAPSLFSAFLYFDVSFMIWVLLGALGAYITADFGLSASQIGFIVGIPILAGSVFRILVGILTDRYGPKKT
SIAGMVVTMVPLLWGWLFGTSLSELIAIGLLLGVAGASFSASLPMASRWYPPHLQGVAMGIAGAGNSGTLFATLFGPRLA
EIVGWNGVMGLALIPLSLVLISYILMAKEPPNQPAPKPLKAYFRVFQERDTWYFCLLYSVTFGGFVGLSSFLSYFFVNQY
AVSGVRAGEFVTLVVAAGSLLRPIGGLIADKIGGARLLTVLFVGVALCMFAVSFLPPIAFALTVLFIGMGCLGMGNGAVF
QLVPQRFHKEIGMVTGIVGAAGGVGGFFLPNILGLFKDITGTYASGFLLFALSACFALGLLLFAQHSWKKAWTETKQTTS
I

Sequences:

>Translated_401_residues
MKLSELRKSGHAPSLFSAFLYFDVSFMIWVLLGALGAYITADFGLSASQIGFIVGIPILAGSVFRILVGILTDRYGPKKT
SIAGMVVTMVPLLWGWLFGTSLSELIAIGLLLGVAGASFSASLPMASRWYPPHLQGVAMGIAGAGNSGTLFATLFGPRLA
EIVGWNGVMGLALIPLSLVLISYILMAKEPPNQPAPKPLKAYFRVFQERDTWYFCLLYSVTFGGFVGLSSFLSYFFVNQY
AVSGVRAGEFVTLVVAAGSLLRPIGGLIADKIGGARLLTVLFVGVALCMFAVSFLPPIAFALTVLFIGMGCLGMGNGAVF
QLVPQRFHKEIGMVTGIVGAAGGVGGFFLPNILGLFKDITGTYASGFLLFALSACFALGLLLFAQHSWKKAWTETKQTTS
I
>Mature_401_residues
MKLSELRKSGHAPSLFSAFLYFDVSFMIWVLLGALGAYITADFGLSASQIGFIVGIPILAGSVFRILVGILTDRYGPKKT
SIAGMVVTMVPLLWGWLFGTSLSELIAIGLLLGVAGASFSASLPMASRWYPPHLQGVAMGIAGAGNSGTLFATLFGPRLA
EIVGWNGVMGLALIPLSLVLISYILMAKEPPNQPAPKPLKAYFRVFQERDTWYFCLLYSVTFGGFVGLSSFLSYFFVNQY
AVSGVRAGEFVTLVVAAGSLLRPIGGLIADKIGGARLLTVLFVGVALCMFAVSFLPPIAFALTVLFIGMGCLGMGNGAVF
QLVPQRFHKEIGMVTGIVGAAGGVGGFFLPNILGLFKDITGTYASGFLLFALSACFALGLLLFAQHSWKKAWTETKQTTS
I

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=424, Percent_Identity=23.8207547169811, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787743, Length=424, Percent_Identity=24.7641509433962, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42581; Mature: 42581

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]