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The map label for this gene is pbpF [H]

Identifier: 56963302

GI number: 56963302

Start: 1658412

End: 1660559

Strand: Direct

Name: pbpF [H]

Synonym: ABC1537

Alternate gene names: 56963302

Gene position: 1658412-1660559 (Clockwise)

Preceding gene: 56963300

Following gene: 56963303

Centisome position: 38.53

GC content: 46.32

Gene sequence:

>2148_bases
ATGGTCAAAAAACTCGCACTAGCGGCTGGCATCGTAGCAGCAATGATGGTGCTTTCCTTTTTAATCTTTGCCGTCGCCAT
ATTGGCGGGCACGTACCAAATCGATGAAGAAAAGCTAATGATGAATAATTCAAGTGTAATCGTTGATACCGACGGCAACG
TCATCACGAACTTATATATGGAAAATCGTGAAGTGATTCCAATTGAACAAGTCCCTGAACTTGTTCAACAAGCATTTGTC
GCAATGGAAGATTCCCGCTTTTATGAACACCAGGGCATTGATTTTAAAGCGATTGGCCGTGCCCTTTACCGTGATATTCT
AGCAGGTGCCAAAGTAGAGGGGGGCAGCACCCTTACACAGCAACTTGCTAAAAATTTGTTTCTCTCCAACGAAAAAGCAT
GGCTACGGAAAACAAATGAAGTGCTTATCGCGATGAATTTAGAGCGGCGTTACAGCAAAAGCGAGATATTGGAAAAGTAC
TTAAATCAAGTTTATTTTGGCCACGGCGCTTATGGGATTGAAGCAGCTGCCCATTTGTATTTTAACAAGCCAGCTTCAGA
ATTAACCATTTATGAAGGAGCCTCCCTTGTAGCTGTCGTGAATGCACCAAGCGCATTTTCGCCAATTGATGAACCGGAGC
GCAACAAAGAGAGACGTGATTTAGTGCTAAAGGTGATGGCGCAACAAGGGTATTTAAGCACTGAGGAAGCTGAGGAAGCG
CAGAGCCAACCGCTGGAAACCGACAGACAAGAGCAAACGGTGGATGAGTCGTTGTATACGTACATTGATATGGTCATGGA
TGAAGCGAGTGAGCGATTCAACATTAGCCATGAAGAGCTGCTAACTGGCGGGTACCGGATTGTGGTTCCAATGAACTTGG
AGTTGCAAAAGAAAACATATGAGGCGCTGCAAAACGATCGTTTCTTCCCAGAAGAAAACAAAGACGCCCAAAGCGCTATG
ATGTTTATGGACTCAGAAACAGGCGGCATTCTCGCTGTCCAAGGGGGCCGTGATTATGTGCGGCGCGGCTTAAACCGCGT
GAATGTAAGGCGGGCGCCTGCTTCAACATTTAAGCCGTTAGCGGTGTTTGCTCCGGCATTAGAAAGTGGCATATATCAGC
CTTATTCGTTGCTGCGCGATGAATTGCTTCAGTACGATGACGGCTATACACCTAAAAATGTAAATGGCGAATATAGTGGC
GAAATGACGATGTATGAAGCACTGACCACATCCTCCAACGCTCCAGCAGTATGGCTTTTAAATGAAATCGGCATTCAGGA
GTCTGTGTCAACGCTCAAAAAGTTCGGCTTTCACTTGCCTGATAGGCAGCTCTCCTTGGCACTCGGAGGCTTGCGAGAAG
GGGTAACTCCTTATGAGATGGTACGTGCTTACCGAGCATTTGCCCATGAAGGACAAATTATTGAACCTCATGTGATTAGA
GACATCTATGACAGCAATGACAAACGTATCGGCGGTGCTAAGCCTGTTGAAACACAAGTCATTAGTGCCCAAACAGCATG
GTCGATGACAAGAATGCTGGAAAATGTCATGGCTGCTGGCACAGGTGCAAGTGGACCTGCAACTACGTCTGCGGTTGCGG
GTAAAACAGGAACGCAAGGATACGAGGCAGTTGCCGGGGCAAACAGCGATGTGTGGTTTGTTGGCTATACGCCAAAAGTG
GTTGGTGCCGTTTGGATGGGGTATGATCAAACAAACGAAAGCCAATACTTCGCCGGGGGAAGTGGCTATCCGACACGGTT
GTTCAAAGAAATGGTCAACTCCTTTCCTGCTTCATTGCAGGCAACGGCGTTCGAACAGCCTGAAGGTACTGCTGATTTAG
CTTCTCCGCTAGCAATGGAAAAGATTGAAGGTTTCACGGCCCAATACACAATTGGCGGCAAAGGTTTAATAAGTGTTCAG
TTAGAATGGGACGCGGTTGAAGATGAGCGTATTGCCTTTAATGTGTATGAAGTCGACGGTGATTCCAAGAAACAAGTTGC
TTCCAAATTAACAGAACCTCATTTTACGATTGGAAAAATGAATCCATTCTCTTTGCCGAAATACCAAGTGGTCACCTATG
ACCTAGAAACGGATACCGAAGGGGCTGCTTCTGAAGCGCTCGAACCTTCGTTTTCATTTTCATTCTAA

Upstream 100 bases:

>100_bases
TTTGTATTTTCCGGTATGATAATTGAAGCATTGGCAAGCAACGTTTACGTGCTTGTGCTAAAGGTCAAGCAAATGCCAGT
GGCCTAAAGGGGGTCATCGG

Downstream 100 bases:

>100_bases
AGCCGCTCCTGTAGCGGCTTCTTTGTTTTTATAGATGGGGCAAATCTCATAAAACTAGGGAATGAACGAAAGCAACATTC
ATTTGAGCCGTAAGATCGCG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MVKKLALAAGIVAAMMVLSFLIFAVAILAGTYQIDEEKLMMNNSSVIVDTDGNVITNLYMENREVIPIEQVPELVQQAFV
AMEDSRFYEHQGIDFKAIGRALYRDILAGAKVEGGSTLTQQLAKNLFLSNEKAWLRKTNEVLIAMNLERRYSKSEILEKY
LNQVYFGHGAYGIEAAAHLYFNKPASELTIYEGASLVAVVNAPSAFSPIDEPERNKERRDLVLKVMAQQGYLSTEEAEEA
QSQPLETDRQEQTVDESLYTYIDMVMDEASERFNISHEELLTGGYRIVVPMNLELQKKTYEALQNDRFFPEENKDAQSAM
MFMDSETGGILAVQGGRDYVRRGLNRVNVRRAPASTFKPLAVFAPALESGIYQPYSLLRDELLQYDDGYTPKNVNGEYSG
EMTMYEALTTSSNAPAVWLLNEIGIQESVSTLKKFGFHLPDRQLSLALGGLREGVTPYEMVRAYRAFAHEGQIIEPHVIR
DIYDSNDKRIGGAKPVETQVISAQTAWSMTRMLENVMAAGTGASGPATTSAVAGKTGTQGYEAVAGANSDVWFVGYTPKV
VGAVWMGYDQTNESQYFAGGSGYPTRLFKEMVNSFPASLQATAFEQPEGTADLASPLAMEKIEGFTAQYTIGGKGLISVQ
LEWDAVEDERIAFNVYEVDGDSKKQVASKLTEPHFTIGKMNPFSLPKYQVVTYDLETDTEGAASEALEPSFSFSF

Sequences:

>Translated_715_residues
MVKKLALAAGIVAAMMVLSFLIFAVAILAGTYQIDEEKLMMNNSSVIVDTDGNVITNLYMENREVIPIEQVPELVQQAFV
AMEDSRFYEHQGIDFKAIGRALYRDILAGAKVEGGSTLTQQLAKNLFLSNEKAWLRKTNEVLIAMNLERRYSKSEILEKY
LNQVYFGHGAYGIEAAAHLYFNKPASELTIYEGASLVAVVNAPSAFSPIDEPERNKERRDLVLKVMAQQGYLSTEEAEEA
QSQPLETDRQEQTVDESLYTYIDMVMDEASERFNISHEELLTGGYRIVVPMNLELQKKTYEALQNDRFFPEENKDAQSAM
MFMDSETGGILAVQGGRDYVRRGLNRVNVRRAPASTFKPLAVFAPALESGIYQPYSLLRDELLQYDDGYTPKNVNGEYSG
EMTMYEALTTSSNAPAVWLLNEIGIQESVSTLKKFGFHLPDRQLSLALGGLREGVTPYEMVRAYRAFAHEGQIIEPHVIR
DIYDSNDKRIGGAKPVETQVISAQTAWSMTRMLENVMAAGTGASGPATTSAVAGKTGTQGYEAVAGANSDVWFVGYTPKV
VGAVWMGYDQTNESQYFAGGSGYPTRLFKEMVNSFPASLQATAFEQPEGTADLASPLAMEKIEGFTAQYTIGGKGLISVQ
LEWDAVEDERIAFNVYEVDGDSKKQVASKLTEPHFTIGKMNPFSLPKYQVVTYDLETDTEGAASEALEPSFSFSF
>Mature_715_residues
MVKKLALAAGIVAAMMVLSFLIFAVAILAGTYQIDEEKLMMNNSSVIVDTDGNVITNLYMENREVIPIEQVPELVQQAFV
AMEDSRFYEHQGIDFKAIGRALYRDILAGAKVEGGSTLTQQLAKNLFLSNEKAWLRKTNEVLIAMNLERRYSKSEILEKY
LNQVYFGHGAYGIEAAAHLYFNKPASELTIYEGASLVAVVNAPSAFSPIDEPERNKERRDLVLKVMAQQGYLSTEEAEEA
QSQPLETDRQEQTVDESLYTYIDMVMDEASERFNISHEELLTGGYRIVVPMNLELQKKTYEALQNDRFFPEENKDAQSAM
MFMDSETGGILAVQGGRDYVRRGLNRVNVRRAPASTFKPLAVFAPALESGIYQPYSLLRDELLQYDDGYTPKNVNGEYSG
EMTMYEALTTSSNAPAVWLLNEIGIQESVSTLKKFGFHLPDRQLSLALGGLREGVTPYEMVRAYRAFAHEGQIIEPHVIR
DIYDSNDKRIGGAKPVETQVISAQTAWSMTRMLENVMAAGTGASGPATTSAVAGKTGTQGYEAVAGANSDVWFVGYTPKV
VGAVWMGYDQTNESQYFAGGSGYPTRLFKEMVNSFPASLQATAFEQPEGTADLASPLAMEKIEGFTAQYTIGGKGLISVQ
LEWDAVEDERIAFNVYEVDGDSKKQVASKLTEPHFTIGKMNPFSLPKYQVVTYDLETDTEGAASEALEPSFSFSF

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=576, Percent_Identity=29.6875, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788867, Length=621, Percent_Identity=28.5024154589372, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI87082258, Length=260, Percent_Identity=35.7692307692308, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789601, Length=172, Percent_Identity=37.7906976744186, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79177; Mature: 79177

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKLALAAGIVAAMMVLSFLIFAVAILAGTYQIDEEKLMMNNSSVIVDTDGNVITNLYM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHEECCCCEEEECCCCEEEEEEE
ENREVIPIEQVPELVQQAFVAMEDSRFYEHQGIDFKAIGRALYRDILAGAKVEGGSTLTQ
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
QLAKNLFLSNEKAWLRKTNEVLIAMNLERRYSKSEILEKYLNQVYFGHGAYGIEAAAHLY
HHHHHHHCCCCHHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHHEECCCCHHHHHHHHEE
FNKPASELTIYEGASLVAVVNAPSAFSPIDEPERNKERRDLVLKVMAQQGYLSTEEAEEA
ECCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
QSQPLETDRQEQTVDESLYTYIDMVMDEASERFNISHEELLTGGYRIVVPMNLELQKKTY
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCHHHHHH
EALQNDRFFPEENKDAQSAMMFMDSETGGILAVQGGRDYVRRGLNRVNVRRAPASTFKPL
HHHHCCCCCCCCCCCHHHHEEEEECCCCCEEEEECCHHHHHCCCCCCCEECCCCCCCCHH
AVFAPALESGIYQPYSLLRDELLQYDDGYTPKNVNGEYSGEMTMYEALTTSSNAPAVWLL
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEEE
NEIGIQESVSTLKKFGFHLPDRQLSLALGGLREGVTPYEMVRAYRAFAHEGQIIEPHVIR
HHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
DIYDSNDKRIGGAKPVETQVISAQTAWSMTRMLENVMAAGTGASGPATTSAVAGKTGTQG
HHHCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCC
YEAVAGANSDVWFVGYTPKVVGAVWMGYDQTNESQYFAGGSGYPTRLFKEMVNSFPASLQ
HHHHCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEE
ATAFEQPEGTADLASPLAMEKIEGFTAQYTIGGKGLISVQLEWDAVEDERIAFNVYEVDG
EEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCCEEEEEEEEECC
DSKKQVASKLTEPHFTIGKMNPFSLPKYQVVTYDLETDTEGAASEALEPSFSFSF
CCHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MVKKLALAAGIVAAMMVLSFLIFAVAILAGTYQIDEEKLMMNNSSVIVDTDGNVITNLYM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHEECCCCEEEECCCCEEEEEEE
ENREVIPIEQVPELVQQAFVAMEDSRFYEHQGIDFKAIGRALYRDILAGAKVEGGSTLTQ
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
QLAKNLFLSNEKAWLRKTNEVLIAMNLERRYSKSEILEKYLNQVYFGHGAYGIEAAAHLY
HHHHHHHCCCCHHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHHEECCCCHHHHHHHHEE
FNKPASELTIYEGASLVAVVNAPSAFSPIDEPERNKERRDLVLKVMAQQGYLSTEEAEEA
ECCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
QSQPLETDRQEQTVDESLYTYIDMVMDEASERFNISHEELLTGGYRIVVPMNLELQKKTY
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCHHHHHH
EALQNDRFFPEENKDAQSAMMFMDSETGGILAVQGGRDYVRRGLNRVNVRRAPASTFKPL
HHHHCCCCCCCCCCCHHHHEEEEECCCCCEEEEECCHHHHHCCCCCCCEECCCCCCCCHH
AVFAPALESGIYQPYSLLRDELLQYDDGYTPKNVNGEYSGEMTMYEALTTSSNAPAVWLL
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEEE
NEIGIQESVSTLKKFGFHLPDRQLSLALGGLREGVTPYEMVRAYRAFAHEGQIIEPHVIR
HHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
DIYDSNDKRIGGAKPVETQVISAQTAWSMTRMLENVMAAGTGASGPATTSAVAGKTGTQG
HHHCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCC
YEAVAGANSDVWFVGYTPKVVGAVWMGYDQTNESQYFAGGSGYPTRLFKEMVNSFPASLQ
HHHHCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEE
ATAFEQPEGTADLASPLAMEKIEGFTAQYTIGGKGLISVQLEWDAVEDERIAFNVYEVDG
EEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCCCEEEEEEEEECC
DSKKQVASKLTEPHFTIGKMNPFSLPKYQVVTYDLETDTEGAASEALEPSFSFSF
CCHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]