| Definition | Bacillus clausii KSM-K16, complete genome. |
|---|---|
| Accession | NC_006582 |
| Length | 4,303,871 |
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The map label for this gene is yngF [H]
Identifier: 56963274
GI number: 56963274
Start: 1634335
End: 1635123
Strand: Direct
Name: yngF [H]
Synonym: ABC1509
Alternate gene names: 56963274
Gene position: 1634335-1635123 (Clockwise)
Preceding gene: 56963273
Following gene: 56963275
Centisome position: 37.97
GC content: 51.84
Gene sequence:
>789_bases TTGAGCTCGAGTAAAACGCTTGTGCAGACAGCAGCTCACGATGGAATTGCCCGTATTACGTTAAACCGTCCACAAGCAGC CAATGCGCTCTCGCTTGCGCTTTTAGATGAGCTCGAAGCGGCTTGCGACTGGGTTGCACAAGAGCACAACATCCGTGTTG TTATTTTAGCTGGCGCAGGCGAAAAAGCGTTTTGTGCCGGCGCTGACTTAAAAGAACGGGCAAACATGACAGAAAGCGAG GCCATTTATGCGGTCAAGCGGATAGGTGAGGTGATTGCACGGGTGGAAAAGCTTCCACAGCCCGTCATTGCCGACGTAGG GGGCAGTGCCTTTGGCGGTGGGTTGGAACTGTGTCTTGCTTGCGATGTCCGCATTTTTGCCGAAGACAGTTTTTATGGTT TGACCGAAACCAAATTGGCGATTATTCCAGGAGCTGGAGGGACGCAGCGGCTGCCTCGTTTAATTGGCTCAGGAAAGGCA AAGGAACTCATTTTCACGGCAAGGCGCTTAACTGGAACAGAAGCCCGTAAATACGGTTTGTGCGAATGGGCACTGCCTCG AGAAAAAACATCCGCAGCTGCTTCACAGTTGGCGGTCGAGATGGCGAGCAACGGCCCGATTGCGTTGCAAGCAGCAAAAA AGGCAATCAACGAAGGGGCAGCCCTTCCTCTTGAAGCCGCTTTGGCTGTGGAACGCGAAGCATATTTGCAGACATTGCAA ACCGAAGATCGTTTAGAAGGGCTTGCTTCATTCAAAGAAAAGCGCCCTCCCGTTTACAAAGGCAAATAG
Upstream 100 bases:
>100_bases TCAATGAAAATGGAGATTCCAATTGAGGCAGAACGCGCAGGGAAAGTCAAGGCGATCGTCAAGCCAGAAGGGGAGTTTGT CAACGAAGGGGACGTTTTGA
Downstream 100 bases:
>100_bases GAGTTGAGAGCGAGATGACATTTGAGCAAACCAAATTGGCGAAGGAAACGGAAATTTTTAAAGGCGGCCATACGAAATAC CATCAACAAAATGCGAAGAA
Product: enoyl-CoA hydratase
Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O
Alternate protein names: NA
Number of amino acids: Translated: 262; Mature: 261
Protein sequence:
>262_residues MSSSKTLVQTAAHDGIARITLNRPQAANALSLALLDELEAACDWVAQEHNIRVVILAGAGEKAFCAGADLKERANMTESE AIYAVKRIGEVIARVEKLPQPVIADVGGSAFGGGLELCLACDVRIFAEDSFYGLTETKLAIIPGAGGTQRLPRLIGSGKA KELIFTARRLTGTEARKYGLCEWALPREKTSAAASQLAVEMASNGPIALQAAKKAINEGAALPLEAALAVEREAYLQTLQ TEDRLEGLASFKEKRPPVYKGK
Sequences:
>Translated_262_residues MSSSKTLVQTAAHDGIARITLNRPQAANALSLALLDELEAACDWVAQEHNIRVVILAGAGEKAFCAGADLKERANMTESE AIYAVKRIGEVIARVEKLPQPVIADVGGSAFGGGLELCLACDVRIFAEDSFYGLTETKLAIIPGAGGTQRLPRLIGSGKA KELIFTARRLTGTEARKYGLCEWALPREKTSAAASQLAVEMASNGPIALQAAKKAINEGAALPLEAALAVEREAYLQTLQ TEDRLEGLASFKEKRPPVYKGK >Mature_261_residues SSSKTLVQTAAHDGIARITLNRPQAANALSLALLDELEAACDWVAQEHNIRVVILAGAGEKAFCAGADLKERANMTESEA IYAVKRIGEVIARVEKLPQPVIADVGGSAFGGGLELCLACDVRIFAEDSFYGLTETKLAIIPGAGGTQRLPRLIGSGKAK ELIFTARRLTGTEARKYGLCEWALPREKTSAAASQLAVEMASNGPIALQAAKKAINEGAALPLEAALAVEREAYLQTLQT EDRLEGLASFKEKRPPVYKGK
Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]
COG id: COG1024
COG function: function code I; Enoyl-CoA hydratase/carnithine racemase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]
Homologues:
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=48.4251968503937, Blast_Score=197, Evalue=7e-51, Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=38.0952380952381, Blast_Score=157, Evalue=1e-38, Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=36, Blast_Score=154, Evalue=5e-38, Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.203007518797, Blast_Score=120, Evalue=2e-27, Organism=Homo sapiens, GI68989263, Length=280, Percent_Identity=29.6428571428571, Blast_Score=107, Evalue=2e-23, Organism=Homo sapiens, GI20127408, Length=212, Percent_Identity=28.7735849056604, Blast_Score=96, Evalue=3e-20, Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=26.8774703557312, Blast_Score=91, Evalue=9e-19, Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=30.2631578947368, Blast_Score=88, Evalue=9e-18, Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=30.2631578947368, Blast_Score=88, Evalue=9e-18, Organism=Homo sapiens, GI37594469, Length=179, Percent_Identity=31.8435754189944, Blast_Score=83, Evalue=2e-16, Organism=Homo sapiens, GI37594471, Length=179, Percent_Identity=31.8435754189944, Blast_Score=83, Evalue=2e-16, Organism=Homo sapiens, GI157694520, Length=184, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=7e-14, Organism=Homo sapiens, GI25453481, Length=247, Percent_Identity=23.8866396761134, Blast_Score=71, Evalue=1e-12, Organism=Homo sapiens, GI51538573, Length=247, Percent_Identity=23.8866396761134, Blast_Score=71, Evalue=1e-12, Organism=Homo sapiens, GI261878539, Length=180, Percent_Identity=31.1111111111111, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI4757966, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI51538581, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI62530384, Length=205, Percent_Identity=28.780487804878, Blast_Score=69, Evalue=6e-12, Organism=Homo sapiens, GI4757968, Length=247, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI49355787, Length=247, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=3e-11, Organism=Homo sapiens, GI221136756, Length=221, Percent_Identity=24.4343891402715, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI221136753, Length=221, Percent_Identity=24.4343891402715, Blast_Score=66, Evalue=4e-11, Organism=Homo sapiens, GI221307494, Length=221, Percent_Identity=24.4343891402715, Blast_Score=65, Evalue=5e-11, Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=39.7540983606557, Blast_Score=167, Evalue=5e-43, Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=34.2105263157895, Blast_Score=142, Evalue=3e-35, Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=32.9317269076305, Blast_Score=130, Evalue=9e-32, Organism=Escherichia coli, GI1788682, Length=215, Percent_Identity=35.8139534883721, Blast_Score=114, Evalue=6e-27, Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=31.4176245210728, Blast_Score=110, Evalue=1e-25, Organism=Escherichia coli, GI1790281, Length=217, Percent_Identity=32.258064516129, Blast_Score=102, Evalue=2e-23, Organism=Escherichia coli, GI87082183, Length=272, Percent_Identity=28.6764705882353, Blast_Score=100, Evalue=1e-22, Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=43.2900432900433, Blast_Score=186, Evalue=2e-47, Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=36.5758754863813, Blast_Score=159, Evalue=1e-39, Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=36.6533864541833, Blast_Score=158, Evalue=3e-39, Organism=Caenorhabditis elegans, GI17534483, Length=262, Percent_Identity=29.0076335877863, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=25.2873563218391, Blast_Score=104, Evalue=4e-23, Organism=Caenorhabditis elegans, GI17558304, Length=174, Percent_Identity=29.8850574712644, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI25144276, Length=155, Percent_Identity=35.4838709677419, Blast_Score=92, Evalue=3e-19, Organism=Caenorhabditis elegans, GI17508951, Length=155, Percent_Identity=35.4838709677419, Blast_Score=92, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17508953, Length=155, Percent_Identity=35.4838709677419, Blast_Score=91, Evalue=4e-19, Organism=Caenorhabditis elegans, GI17560910, Length=217, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17549921, Length=210, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=6e-15, Organism=Caenorhabditis elegans, GI17540306, Length=264, Percent_Identity=25.7575757575758, Blast_Score=77, Evalue=1e-14, Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=24.5, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI25144157, Length=185, Percent_Identity=30.8108108108108, Blast_Score=74, Evalue=9e-14, Organism=Caenorhabditis elegans, GI25144160, Length=185, Percent_Identity=30.8108108108108, Blast_Score=74, Evalue=9e-14, Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=3e-12, Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=47.843137254902, Blast_Score=229, Evalue=2e-60, Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=38.2113821138211, Blast_Score=158, Evalue=4e-39, Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=38.2113821138211, Blast_Score=158, Evalue=4e-39, Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=31.4410480349345, Blast_Score=106, Evalue=1e-23, Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=27.5590551181102, Blast_Score=105, Evalue=4e-23, Organism=Drosophila melanogaster, GI24650670, Length=251, Percent_Identity=28.2868525896414, Blast_Score=87, Evalue=1e-17, Organism=Drosophila melanogaster, GI21357171, Length=252, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=9e-16, Organism=Drosophila melanogaster, GI28571730, Length=182, Percent_Identity=30.2197802197802, Blast_Score=77, Evalue=1e-14, Organism=Drosophila melanogaster, GI28571729, Length=182, Percent_Identity=30.2197802197802, Blast_Score=77, Evalue=1e-14, Organism=Drosophila melanogaster, GI24654903, Length=162, Percent_Identity=24.0740740740741, Blast_Score=68, Evalue=7e-12, Organism=Drosophila melanogaster, GI19921000, Length=180, Percent_Identity=30.5555555555556, Blast_Score=66, Evalue=3e-11, Organism=Drosophila melanogaster, GI24583077, Length=180, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=3e-11, Organism=Drosophila melanogaster, GI24583079, Length=180, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=3e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014748 - InterPro: IPR001753 - InterPro: IPR018376 [H]
Pfam domain/function: PF00378 ECH [H]
EC number: 4.2.1.17
Molecular weight: Translated: 27845; Mature: 27714
Theoretical pI: Translated: 7.25; Mature: 7.25
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 0.8 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSSKTLVQTAAHDGIARITLNRPQAANALSLALLDELEAACDWVAQEHNIRVVILAGAG CCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC EKAFCAGADLKERANMTESEAIYAVKRIGEVIARVEKLPQPVIADVGGSAFGGGLELCLA CCEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHEE CDVRIFAEDSFYGLTETKLAIIPGAGGTQRLPRLIGSGKAKELIFTARRLTGTEARKYGL CCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCC CEWALPREKTSAAASQLAVEMASNGPIALQAAKKAINEGAALPLEAALAVEREAYLQTLQ CCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH TEDRLEGLASFKEKRPPVYKGK HHHHHHHHHHHHHHCCCCCCCC >Mature Secondary Structure SSSKTLVQTAAHDGIARITLNRPQAANALSLALLDELEAACDWVAQEHNIRVVILAGAG CCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC EKAFCAGADLKERANMTESEAIYAVKRIGEVIARVEKLPQPVIADVGGSAFGGGLELCLA CCEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHEE CDVRIFAEDSFYGLTETKLAIIPGAGGTQRLPRLIGSGKAKELIFTARRLTGTEARKYGL CCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCC CEWALPREKTSAAASQLAVEMASNGPIALQAAKKAINEGAALPLEAALAVEREAYLQTLQ CCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH TEDRLEGLASFKEKRPPVYKGK HHHHHHHHHHHHHHCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]
Substrates: (3S)-3-hydroxyacyl-CoA
Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O
General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9387222; 9384377 [H]