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The map label for this gene is metC [H]

Identifier: 56963214

GI number: 56963214

Start: 1564043

End: 1566328

Strand: Direct

Name: metC [H]

Synonym: ABC1449

Alternate gene names: 56963214

Gene position: 1564043-1566328 (Clockwise)

Preceding gene: 56963209

Following gene: 56963217

Centisome position: 36.34

GC content: 46.54

Gene sequence:

>2286_bases
GTGAGAAGTTCATCATTAGGGTATCCGCGGATTGGCGGCCAGCGAGAATGGAAAAAAAACCTTGAGGCGTTTTGGGCAGG
AAAGCGCTCAAAAACTGATTTTTTGCAGGAACAGAAATCGTTAAGGCTATCTTATTTGCAAAAACAGCAACAGCTCGGCC
TTGATCTCATTCCGGTAGGCGATTTTAGTGATTATGACCATGTGTTAGACACGGCTGTTGCTTTCGGAATTGTGCCAGAA
AGATTTGCGTATGCTGGCGGAAAAGTGGAGGAGGAAACGTATTTTGCGATTGCCCGGGGAACGGATGATGCTGTCGCAGC
CGAAATGACAAAGTGGTTCAACACCAACTACCACTATATTGTCCCAGAGTTAACGGCTAGCAGAGAACCAGCCCTTGTCG
ATAACCGCTGGCTACGTTTGTACGAAGAGGCAAAAAGTGAACTTGGCATTAACGGCAAACCGGTACTTCTTGGGCCAGTC
AGTTTTGTGAAACTAGCAAAACAATATGGAAATAAATCCTTCTCCGAACATGTGCAGACACTTGTGCCTTTGTACGGACA
AGTACTGGCTGAATTGGCACAGGCAGGCGCGCCATTGATTCAAGTAGATGAACCGATTTTGGCTGGCGATGTGACCGAAG
CAGAGTGGGCTGTTGTCGAGGAAACGTATCAGTCATTAGCGAAACTAGCGCCAAACGCCAAGCTTTTGCTGCAAACCTAT
TTTGAGAGCGTCGCTGACTATAACCGTTTTATACAGCTGCCAGTTGCGGGGCTAGGATTTGACTTTGTAGCTGGAAAAGA
AGAAACATTTCAAAATCTTCGTGTAAACGGATTTCCAAACGATAAGCTGTTAGCACTTGGCGTTATTGATGGACGTAACA
TTTGGCGGACGGATCTTACAGCTGTCAATGCCGAACTAAAGGAAGTGTTACAGGTAGTTGAACGGGAAAGGCTCATTCTC
CAACCATCATCTAGCCTTTTGCATGTGCCAGTAACGGTCCAATCAGAAGAGAAAATAGCAGATGAATTAAAAGAAGCGAT
TGCATTTGCGGATGAAAAAATAATCGAGATCGTTTCATTGGCGAAAGTGGTCAACGGTCACGAGCCACCTTCGTTATTAG
AAGCGAGCAAACACGCGTTAGGGCGATTAAATGCTTCAGAAGCCCGGAACAATCAATCGGTCCAACAAGAAGTAGCACAA
ATCAGCAGCGAGGACCCATCCCGTGGTCTAGACGCACCTACGCGTGCCAAGTTGCAAGCGGCCAAACTGAAACTGCCGCT
GTTGCCGACGACGACGATTGGCAGTTTTCCACAAACAAAAGAGATCCGTAAAAAACGGTTAGACTGGCGCAAAGGAAAAT
TGGACAGTGCTGGCTATGAAACATTCATTAACGCCGAGATAGACCGCTGGATCGCTATCCAAGAACAGCTTGGGCTCGAC
GTCCTTGTCCATGGCGAATTTGAACGGACAGACATGGTTGAATTTTTTGGCGAAAAGCTTGGCGGCTTCCAGTTTACTAC
ATATGGCTGGGTTCAGTCTTATGGGTCACGTTGTGTCAAACCGCCAATTATTTACGGAGATGTTTACTTTAAACAACCAA
TGACTGTAAAAGAAACGGTTTATGCCCAATCCAAAACAGACAAACCGGTTAAGGGGATGCTAACTGGTCCAGTCACGATT
TACAATTGGTCGTTTCCAAGGGCTGATATTACGGAAGGGGAGGCGGTTAATCAAATTGCTCTCGCTTTGCGCAAAGAAGT
CCTTGCCCTTGAAAAACATGGAATTGGCGTCATTCAAGTAGATGAACCTGCTTTGCGGGAAGGATTGCCGCTTAAGCGCG
CGAAATGGCAAAGCTATCTTGACCAATCGGTAACGGCCTTTCAGTTGACGGTCAACCAAGTAAAGCCAGAAACGCAAATC
CATACGCATATGTGTTATTCTGATTTCCAAGATATTATCGAAGCGATTGACGGGCTTGACGCAGACGTCATTTCAATCGA
AATGTCCCGTAGCCACGGGGAACTAATTTCATCATTCGAAGAGTACACGTATAACAAAGGCATTGGCCTCGGTGTATATG
ACATTCACAGCCCCCGCGTTCCGGCTAAAGAAGAAATTGCCCGTAACATTGAGCGTGCGCTGAATGTCTTACATCCAAAG
CAATTTTGGGTCAATCCTGACTGCGGCTTGAAAACACGGCAAGAACCAGAAACAGTGGCTGCATTAAAGGTGATGGTGGA
GGCAGCGAAGGAAGCCCGGCAAACACTTGAGACGAAGACCATTTAA

Upstream 100 bases:

>100_bases
TTAACAATTGGCTAACTTTCCAAATCCCTTTTAATTACGTAATGGGGATTTTTTGTTTGTCGATGATAAAAACATGAAGG
GGTGGAGAAATTTGAGCAAA

Downstream 100 bases:

>100_bases
CTTTTTGGCAGCAAAGCATTCAAACGTTTGACAAGCGTTTTCTATCAGTCTGAAGGGCATTATCGACTTACTTTGTTAAT
AACTTCAGCATGGGGCAGAC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 761; Mature: 761

Protein sequence:

>761_residues
MRSSSLGYPRIGGQREWKKNLEAFWAGKRSKTDFLQEQKSLRLSYLQKQQQLGLDLIPVGDFSDYDHVLDTAVAFGIVPE
RFAYAGGKVEEETYFAIARGTDDAVAAEMTKWFNTNYHYIVPELTASREPALVDNRWLRLYEEAKSELGINGKPVLLGPV
SFVKLAKQYGNKSFSEHVQTLVPLYGQVLAELAQAGAPLIQVDEPILAGDVTEAEWAVVEETYQSLAKLAPNAKLLLQTY
FESVADYNRFIQLPVAGLGFDFVAGKEETFQNLRVNGFPNDKLLALGVIDGRNIWRTDLTAVNAELKEVLQVVERERLIL
QPSSSLLHVPVTVQSEEKIADELKEAIAFADEKIIEIVSLAKVVNGHEPPSLLEASKHALGRLNASEARNNQSVQQEVAQ
ISSEDPSRGLDAPTRAKLQAAKLKLPLLPTTTIGSFPQTKEIRKKRLDWRKGKLDSAGYETFINAEIDRWIAIQEQLGLD
VLVHGEFERTDMVEFFGEKLGGFQFTTYGWVQSYGSRCVKPPIIYGDVYFKQPMTVKETVYAQSKTDKPVKGMLTGPVTI
YNWSFPRADITEGEAVNQIALALRKEVLALEKHGIGVIQVDEPALREGLPLKRAKWQSYLDQSVTAFQLTVNQVKPETQI
HTHMCYSDFQDIIEAIDGLDADVISIEMSRSHGELISSFEEYTYNKGIGLGVYDIHSPRVPAKEEIARNIERALNVLHPK
QFWVNPDCGLKTRQEPETVAALKVMVEAAKEARQTLETKTI

Sequences:

>Translated_761_residues
MRSSSLGYPRIGGQREWKKNLEAFWAGKRSKTDFLQEQKSLRLSYLQKQQQLGLDLIPVGDFSDYDHVLDTAVAFGIVPE
RFAYAGGKVEEETYFAIARGTDDAVAAEMTKWFNTNYHYIVPELTASREPALVDNRWLRLYEEAKSELGINGKPVLLGPV
SFVKLAKQYGNKSFSEHVQTLVPLYGQVLAELAQAGAPLIQVDEPILAGDVTEAEWAVVEETYQSLAKLAPNAKLLLQTY
FESVADYNRFIQLPVAGLGFDFVAGKEETFQNLRVNGFPNDKLLALGVIDGRNIWRTDLTAVNAELKEVLQVVERERLIL
QPSSSLLHVPVTVQSEEKIADELKEAIAFADEKIIEIVSLAKVVNGHEPPSLLEASKHALGRLNASEARNNQSVQQEVAQ
ISSEDPSRGLDAPTRAKLQAAKLKLPLLPTTTIGSFPQTKEIRKKRLDWRKGKLDSAGYETFINAEIDRWIAIQEQLGLD
VLVHGEFERTDMVEFFGEKLGGFQFTTYGWVQSYGSRCVKPPIIYGDVYFKQPMTVKETVYAQSKTDKPVKGMLTGPVTI
YNWSFPRADITEGEAVNQIALALRKEVLALEKHGIGVIQVDEPALREGLPLKRAKWQSYLDQSVTAFQLTVNQVKPETQI
HTHMCYSDFQDIIEAIDGLDADVISIEMSRSHGELISSFEEYTYNKGIGLGVYDIHSPRVPAKEEIARNIERALNVLHPK
QFWVNPDCGLKTRQEPETVAALKVMVEAAKEARQTLETKTI
>Mature_761_residues
MRSSSLGYPRIGGQREWKKNLEAFWAGKRSKTDFLQEQKSLRLSYLQKQQQLGLDLIPVGDFSDYDHVLDTAVAFGIVPE
RFAYAGGKVEEETYFAIARGTDDAVAAEMTKWFNTNYHYIVPELTASREPALVDNRWLRLYEEAKSELGINGKPVLLGPV
SFVKLAKQYGNKSFSEHVQTLVPLYGQVLAELAQAGAPLIQVDEPILAGDVTEAEWAVVEETYQSLAKLAPNAKLLLQTY
FESVADYNRFIQLPVAGLGFDFVAGKEETFQNLRVNGFPNDKLLALGVIDGRNIWRTDLTAVNAELKEVLQVVERERLIL
QPSSSLLHVPVTVQSEEKIADELKEAIAFADEKIIEIVSLAKVVNGHEPPSLLEASKHALGRLNASEARNNQSVQQEVAQ
ISSEDPSRGLDAPTRAKLQAAKLKLPLLPTTTIGSFPQTKEIRKKRLDWRKGKLDSAGYETFINAEIDRWIAIQEQLGLD
VLVHGEFERTDMVEFFGEKLGGFQFTTYGWVQSYGSRCVKPPIIYGDVYFKQPMTVKETVYAQSKTDKPVKGMLTGPVTI
YNWSFPRADITEGEAVNQIALALRKEVLALEKHGIGVIQVDEPALREGLPLKRAKWQSYLDQSVTAFQLTVNQVKPETQI
HTHMCYSDFQDIIEAIDGLDADVISIEMSRSHGELISSFEEYTYNKGIGLGVYDIHSPRVPAKEEIARNIERALNVLHPK
QFWVNPDCGLKTRQEPETVAALKVMVEAAKEARQTLETKTI

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=760, Percent_Identity=47.8947368421053, Blast_Score=671, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=771, Percent_Identity=43.0609597924773, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85226; Mature: 85226

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSSLGYPRIGGQREWKKNLEAFWAGKRSKTDFLQEQKSLRLSYLQKQQQLGLDLIPVG
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
DFSDYDHVLDTAVAFGIVPERFAYAGGKVEEETYFAIARGTDDAVAAEMTKWFNTNYHYI
CCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEE
VPELTASREPALVDNRWLRLYEEAKSELGINGKPVLLGPVSFVKLAKQYGNKSFSEHVQT
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHH
LVPLYGQVLAELAQAGAPLIQVDEPILAGDVTEAEWAVVEETYQSLAKLAPNAKLLLQTY
HHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
FESVADYNRFIQLPVAGLGFDFVAGKEETFQNLRVNGFPNDKLLALGVIDGRNIWRTDLT
HHHHHHHHHEEECCCCCCCCHHHCCCHHHHHCCEECCCCCCCEEEEEEECCCCCCHHHHH
AVNAELKEVLQVVERERLILQPSSSLLHVPVTVQSEEKIADELKEAIAFADEKIIEIVSL
HHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
AKVVNGHEPPSLLEASKHALGRLNASEARNNQSVQQEVAQISSEDPSRGLDAPTRAKLQA
HHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AKLKLPLLPTTTIGSFPQTKEIRKKRLDWRKGKLDSAGYETFINAEIDRWIAIQEQLGLD
HHEECCCCCCCCCCCCCCHHHHHHHHCCHHCCCCCCCCCHHEECCCHHHHHHHHHHCCCE
VLVHGEFERTDMVEFFGEKLGGFQFTTYGWVQSYGSRCVKPPIIYGDVYFKQPMTVKETV
EEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCEEECCEEECCCCCHHHHH
YAQSKTDKPVKGMLTGPVTIYNWSFPRADITEGEAVNQIALALRKEVLALEKHGIGVIQV
HHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
DEPALREGLPLKRAKWQSYLDQSVTAFQLTVNQVKPETQIHTHMCYSDFQDIIEAIDGLD
CCHHHHCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
ADVISIEMSRSHGELISSFEEYTYNKGIGLGVYDIHSPRVPAKEEIARNIERALNVLHPK
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCH
QFWVNPDCGLKTRQEPETVAALKVMVEAAKEARQTLETKTI
HEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSSSLGYPRIGGQREWKKNLEAFWAGKRSKTDFLQEQKSLRLSYLQKQQQLGLDLIPVG
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
DFSDYDHVLDTAVAFGIVPERFAYAGGKVEEETYFAIARGTDDAVAAEMTKWFNTNYHYI
CCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEE
VPELTASREPALVDNRWLRLYEEAKSELGINGKPVLLGPVSFVKLAKQYGNKSFSEHVQT
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHH
LVPLYGQVLAELAQAGAPLIQVDEPILAGDVTEAEWAVVEETYQSLAKLAPNAKLLLQTY
HHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
FESVADYNRFIQLPVAGLGFDFVAGKEETFQNLRVNGFPNDKLLALGVIDGRNIWRTDLT
HHHHHHHHHEEECCCCCCCCHHHCCCHHHHHCCEECCCCCCCEEEEEEECCCCCCHHHHH
AVNAELKEVLQVVERERLILQPSSSLLHVPVTVQSEEKIADELKEAIAFADEKIIEIVSL
HHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
AKVVNGHEPPSLLEASKHALGRLNASEARNNQSVQQEVAQISSEDPSRGLDAPTRAKLQA
HHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AKLKLPLLPTTTIGSFPQTKEIRKKRLDWRKGKLDSAGYETFINAEIDRWIAIQEQLGLD
HHEECCCCCCCCCCCCCCHHHHHHHHCCHHCCCCCCCCCHHEECCCHHHHHHHHHHCCCE
VLVHGEFERTDMVEFFGEKLGGFQFTTYGWVQSYGSRCVKPPIIYGDVYFKQPMTVKETV
EEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCEEECCEEECCCCCHHHHH
YAQSKTDKPVKGMLTGPVTIYNWSFPRADITEGEAVNQIALALRKEVLALEKHGIGVIQV
HHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
DEPALREGLPLKRAKWQSYLDQSVTAFQLTVNQVKPETQIHTHMCYSDFQDIIEAIDGLD
CCHHHHCCCCCHHHHHHHHHHCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
ADVISIEMSRSHGELISSFEEYTYNKGIGLGVYDIHSPRVPAKEEIARNIERALNVLHPK
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCH
QFWVNPDCGLKTRQEPETVAALKVMVEAAKEARQTLETKTI
HEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]