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The map label for this gene is pfkA

Identifier: 55981931

GI number: 55981931

Start: 1838240

End: 1839208

Strand: Reverse

Name: pfkA

Synonym: TTHA1962

Alternate gene names: 55981931

Gene position: 1839208-1838240 (Counterclockwise)

Preceding gene: 55981932

Following gene: 55981926

Centisome position: 99.43

GC content: 71.93

Gene sequence:

>969_bases
ATGAAACGCATCGGGGTGTTCACCAGCGGCGGCGACGCGCCGGGGATGAACGCGGCCATCCGGGCGGTGGTGCGCCAGGC
CCACGCCCTGGGGGTGGAGGTCATCGGGATCCGCCGCGGCTACGCCGGCATGATCCAAGGGGAGATGGTGCCCTTGGGGG
TGCGGGACGTGGCCAACATCATCCAGCGGGGCGGGACGATCCTCCTCACGGCGAGGAGCCAGGAGTTCCTCACGGAGGAG
GGGCGGGCCAAGGCCTACGCCAAGCTGCAGGCGGCGGGGATTGAGGGCCTGGTGGCCATCGGCGGCGACGGGACCTTCCG
CGGGGCGCTTTGCCTCGTGGAGGAGCACGGGATGCCGGTGGTGGGGGTTCCGGGCACCATAGACAACGACCTCTACGGCA
CGGACTACACCATCGGCTTTGACACCGCGGTGAACACGGCCCTCGAGGCCATAGACCGGATCCGGGACACCGCGGCGAGC
CACGAGCGGGTCTTCTTCATAGAGGTCATGGGGCGGCACGCCGGGTTCATCGCCCTGGACGTGGGGCTTGCCGGCGGGGC
GGAGGTCATCGCCGTCCCCGAGGAGCCCGTGGACCCCAAGGCCGTGGCCGAGGTGCTGGAGGCCTCCCAGAGGCGGGGGA
AGAAGAGCTCCATCGTGGTGGTGGCCGAGGGGGCCTACCCCGGCGGGGCCGCGGGGCTTCTCGCCGCCATCCGGGAGCAC
CTCCAGGTGGAGGCCCGGGTCACCGTCCTGGGGCACATCCAGCGGGGCGGGAGCCCCACGGCCAAGGACCGGATCCTGGC
GAGCCGCCTGGGGGCGGCCGCGGTGGAGGCCCTGGTGGGCGGGGCGAGCGGGGTCATGGTGGGGGAGGTGGAGGGCGAGG
TGGACCTCACCCCCCTGAAGGAGGCGGTGGAGCGCAGGAAGGACATCAACCGGGCCCTCTTGCGCCTATCGCAGGTGCTG
GCCCTCTAG

Upstream 100 bases:

>100_bases
AGGAGGAGGGGCTTTCGGGGAGGGCCTTGGTCCGGGCCCTCGTGGCCTTGGGCCTTCCCCGGAACGAGGCGTACCGACTG
GCCTTGGAGGAGGAAGAGGG

Downstream 100 bases:

>100_bases
GCCCAACGCCTTCCGCGCCCAGGGCGTTCCCCAGGCCCAGGCGAAGACCCGGCGGGCGAAGGGGCTTTGCGCCACCCCCT
GGTAGATGGGGGTGGGCAGG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: PFK1; Phosphofructokinase 1; Phosphohexokinase 1

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKRIGVFTSGGDAPGMNAAIRAVVRQAHALGVEVIGIRRGYAGMIQGEMVPLGVRDVANIIQRGGTILLTARSQEFLTEE
GRAKAYAKLQAAGIEGLVAIGGDGTFRGALCLVEEHGMPVVGVPGTIDNDLYGTDYTIGFDTAVNTALEAIDRIRDTAAS
HERVFFIEVMGRHAGFIALDVGLAGGAEVIAVPEEPVDPKAVAEVLEASQRRGKKSSIVVVAEGAYPGGAAGLLAAIREH
LQVEARVTVLGHIQRGGSPTAKDRILASRLGAAAVEALVGGASGVMVGEVEGEVDLTPLKEAVERRKDINRALLRLSQVL
AL

Sequences:

>Translated_322_residues
MKRIGVFTSGGDAPGMNAAIRAVVRQAHALGVEVIGIRRGYAGMIQGEMVPLGVRDVANIIQRGGTILLTARSQEFLTEE
GRAKAYAKLQAAGIEGLVAIGGDGTFRGALCLVEEHGMPVVGVPGTIDNDLYGTDYTIGFDTAVNTALEAIDRIRDTAAS
HERVFFIEVMGRHAGFIALDVGLAGGAEVIAVPEEPVDPKAVAEVLEASQRRGKKSSIVVVAEGAYPGGAAGLLAAIREH
LQVEARVTVLGHIQRGGSPTAKDRILASRLGAAAVEALVGGASGVMVGEVEGEVDLTPLKEAVERRKDINRALLRLSQVL
AL
>Mature_322_residues
MKRIGVFTSGGDAPGMNAAIRAVVRQAHALGVEVIGIRRGYAGMIQGEMVPLGVRDVANIIQRGGTILLTARSQEFLTEE
GRAKAYAKLQAAGIEGLVAIGGDGTFRGALCLVEEHGMPVVGVPGTIDNDLYGTDYTIGFDTAVNTALEAIDRIRDTAAS
HERVFFIEVMGRHAGFIALDVGLAGGAEVIAVPEEPVDPKAVAEVLEASQRRGKKSSIVVVAEGAYPGGAAGLLAAIREH
LQVEARVTVLGHIQRGGSPTAKDRILASRLGAAAVEALVGGASGVMVGEVEGEVDLTPLKEAVERRKDINRALLRLSQVL
AL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=347, Percent_Identity=40.6340057636888, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI48762920, Length=347, Percent_Identity=39.7694524495677, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI266453619, Length=347, Percent_Identity=38.328530259366, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI266453768, Length=347, Percent_Identity=38.328530259366, Blast_Score=214, Evalue=9e-56,
Organism=Homo sapiens, GI266453748, Length=347, Percent_Identity=38.328530259366, Blast_Score=214, Evalue=9e-56,
Organism=Homo sapiens, GI4505749, Length=347, Percent_Identity=38.328530259366, Blast_Score=214, Evalue=9e-56,
Organism=Escherichia coli, GI1790350, Length=307, Percent_Identity=48.5342019543974, Blast_Score=265, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI25147584, Length=348, Percent_Identity=41.3793103448276, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=37.741935483871, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI72003601, Length=290, Percent_Identity=31.7241379310345, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321679, Length=348, Percent_Identity=38.2183908045977, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6323861, Length=349, Percent_Identity=35.5300859598854, Blast_Score=189, Evalue=6e-49,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=42.0749279538905, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17647809, Length=347, Percent_Identity=43.2276657060519, Blast_Score=237, Evalue=6e-63,
Organism=Drosophila melanogaster, GI24652337, Length=347, Percent_Identity=43.2276657060519, Blast_Score=237, Evalue=8e-63,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF1_THET8 (P21777)

Other databases:

- EMBL:   M71213
- EMBL:   AP008226
- RefSeq:   YP_145228.1
- ProteinModelPortal:   P21777
- SMR:   P21777
- STRING:   P21777
- GeneID:   3169721
- GenomeReviews:   AP008226_GR
- KEGG:   ttj:TTHA1962
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- ProtClustDB:   PRK03202
- BioCyc:   TTHE300852:TTHA1962-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 33537; Mature: 33537

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 246-246 BINDING 252-252 BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVFTSGGDAPGMNAAIRAVVRQAHALGVEVIGIRRGYAGMIQGEMVPLGVRDVANI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEECHHHHHHH
IQRGGTILLTARSQEFLTEEGRAKAYAKLQAAGIEGLVAIGGDGTFRGALCLVEEHGMPV
HHCCCEEEEEECCHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCE
VGVPGTIDNDLYGTDYTIGFDTAVNTALEAIDRIRDTAASHERVFFIEVMGRHAGFIALD
EECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEE
VGLAGGAEVIAVPEEPVDPKAVAEVLEASQRRGKKSSIVVVAEGAYPGGAAGLLAAIREH
ECCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
LQVEARVTVLGHIQRGGSPTAKDRILASRLGAAAVEALVGGASGVMVGEVEGEVDLTPLK
HHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHH
EAVERRKDINRALLRLSQVLAL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRIGVFTSGGDAPGMNAAIRAVVRQAHALGVEVIGIRRGYAGMIQGEMVPLGVRDVANI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEECHHHHHHH
IQRGGTILLTARSQEFLTEEGRAKAYAKLQAAGIEGLVAIGGDGTFRGALCLVEEHGMPV
HHCCCEEEEEECCHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCE
VGVPGTIDNDLYGTDYTIGFDTAVNTALEAIDRIRDTAASHERVFFIEVMGRHAGFIALD
EECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEE
VGLAGGAEVIAVPEEPVDPKAVAEVLEASQRRGKKSSIVVVAEGAYPGGAAGLLAAIREH
ECCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
LQVEARVTVLGHIQRGGSPTAKDRILASRLGAAAVEALVGGASGVMVGEVEGEVDLTPLK
HHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHH
EAVERRKDINRALLRLSQVLAL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1828151; 1830879