Click here to switch to the map view.

The map label for this gene is proB

Identifier: 55981896

GI number: 55981896

Start: 1807406

End: 1808518

Strand: Direct

Name: proB

Synonym: TTHA1927

Alternate gene names: 55981896

Gene position: 1807406-1808518 (Clockwise)

Preceding gene: 55981894

Following gene: 55981897

Centisome position: 97.71

GC content: 70.35

Gene sequence:

>1113_bases
ATGCGCCCGGGGCTTTCGGCCAAGCGCCTGGTGGTCAAGGTGGGAAGCGCCGTCCTCACAGGGGAAAGGGGCCTGGACCT
CGAGGCCATGGCCGAGATCGCCCGCCAGGTGGCCGCCCTGAGGGAGGAAGGCCGGGAGGTGGTCCTCGTCTCCTCGGGGG
CGGTGGCGGCGGGAATGCGACGGCTCGGGCTAAAGGAGCGCCCCAAGGACATGCCCAAGAAGCAGGCCCTCGCCGCCCTC
GGCCAGCCCCTCCTCATGGCCTTCTGGCAAGAGGCCTTCGCCCCCTTCGGCCTTCCCGTGGCCCAGGTCCTCCTCACCGC
CGAGGACCTCTCCTCCAGGAGCCGCTACCTGAACGCCAAGGCCACCTTGCGGGCCCTCCTGGACCTGGGGGCCATACCCG
TGATCAACGAAAACGACACCGTGGCCTTTGAGGAGATCCGCTTTGGGGACAACGACCAGCTCTCCGCCCGGGTGGCGGCC
CTGGTGGAGGCGGGCCTCCTCGCCCTCCTCTCCGATGTGGACGCCCTCTACGAGGAGGACCCCAAGAAAAACCCCCAGGC
CCGCCCCATCCCCGAGGTGGAGTCCGTGGAGGCGGTTTTGGCCCACGCCGGAGAGGAAAACCCGCTAGGAAGCGGGGGGA
TGAAGAGCAAGCTCCTCGCCGCCCGCATCGCCGGTAGGGTGGGGATCCCCACCCTGCTTCTCCCCGGGAAGCGGCCAGGG
GTCCTCCTCCAAGCCCTCTCCGGGGCCCCCCTGGGCACCTACTTCCACGCGCGGCGGCGCTACCGGGGGGAAAAGGCCTG
GCTTTTTGGCCTCCTCCGCCCCAAGGGGGAGCTCGTCCTGGACCGGGGGGCGGTGCGGGCCTTAAAGGAGCGGGGAGCGA
GCCTGCTTCCCGCCGGGGTTAAGGAGGTGCGGGGGAGGTTCTCCCGGGGCGAGGCGGTCCGCCTCCTCTCGGAGGAGGGC
GAGGAGGTGGGGGTGGGGCTCGCCAACTACGCCTCGGAGGAGATCGCCCGCATCAAGGGGCGGCGGAGCGCGGAGATTGA
GGCCGTCCTGGGCTACCGCTACACCGAGGAGGTGGTCCACCGGGACCACCTCGCCTTGAAGGAGGAGGCATGA

Upstream 100 bases:

>100_bases
CCAGGGCGAAGCGGCTCTCCCCACCCCGGTGCCAGAGGCCGAGGGCGGTGCGGGTGCCGTCTTCGGCCAAGTGGTGAGGG
AGGTGCTATCCTGGGCCTTA

Downstream 100 bases:

>100_bases
CGGCAACAAGCCTTTGGGAACTGGCGGAGCGCGCGCGCAAGAGGCTTTCCGAGATCGCGAAGGGGAACCGGGACCGGGCC
CTTCTCGCCATGGCGGACCT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MRPGLSAKRLVVKVGSAVLTGERGLDLEAMAEIARQVAALREEGREVVLVSSGAVAAGMRRLGLKERPKDMPKKQALAAL
GQPLLMAFWQEAFAPFGLPVAQVLLTAEDLSSRSRYLNAKATLRALLDLGAIPVINENDTVAFEEIRFGDNDQLSARVAA
LVEAGLLALLSDVDALYEEDPKKNPQARPIPEVESVEAVLAHAGEENPLGSGGMKSKLLAARIAGRVGIPTLLLPGKRPG
VLLQALSGAPLGTYFHARRRYRGEKAWLFGLLRPKGELVLDRGAVRALKERGASLLPAGVKEVRGRFSRGEAVRLLSEEG
EEVGVGLANYASEEIARIKGRRSAEIEAVLGYRYTEEVVHRDHLALKEEA

Sequences:

>Translated_370_residues
MRPGLSAKRLVVKVGSAVLTGERGLDLEAMAEIARQVAALREEGREVVLVSSGAVAAGMRRLGLKERPKDMPKKQALAAL
GQPLLMAFWQEAFAPFGLPVAQVLLTAEDLSSRSRYLNAKATLRALLDLGAIPVINENDTVAFEEIRFGDNDQLSARVAA
LVEAGLLALLSDVDALYEEDPKKNPQARPIPEVESVEAVLAHAGEENPLGSGGMKSKLLAARIAGRVGIPTLLLPGKRPG
VLLQALSGAPLGTYFHARRRYRGEKAWLFGLLRPKGELVLDRGAVRALKERGASLLPAGVKEVRGRFSRGEAVRLLSEEG
EEVGVGLANYASEEIARIKGRRSAEIEAVLGYRYTEEVVHRDHLALKEEA
>Mature_370_residues
MRPGLSAKRLVVKVGSAVLTGERGLDLEAMAEIARQVAALREEGREVVLVSSGAVAAGMRRLGLKERPKDMPKKQALAAL
GQPLLMAFWQEAFAPFGLPVAQVLLTAEDLSSRSRYLNAKATLRALLDLGAIPVINENDTVAFEEIRFGDNDQLSARVAA
LVEAGLLALLSDVDALYEEDPKKNPQARPIPEVESVEAVLAHAGEENPLGSGGMKSKLLAARIAGRVGIPTLLLPGKRPG
VLLQALSGAPLGTYFHARRRYRGEKAWLFGLLRPKGELVLDRGAVRALKERGASLLPAGVKEVRGRFSRGEAVRLLSEEG
EEVGVGLANYASEEIARIKGRRSAEIEAVLGYRYTEEVVHRDHLALKEEA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=285, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI62912457, Length=283, Percent_Identity=33.5689045936396, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=46.5564738292011, Blast_Score=313, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=37.2262773722628, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=37.2262773722628, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=35.7487922705314, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321822, Length=416, Percent_Identity=36.0576923076923, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI21357643, Length=282, Percent_Identity=36.5248226950355, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_THET2 (Q60050)

Other databases:

- EMBL:   D29973
- EMBL:   AE017221
- RefSeq:   YP_005532.1
- ProteinModelPortal:   Q60050
- SMR:   Q60050
- STRING:   Q60050
- GeneID:   2776015
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q60050
- ProtClustDB:   PRK05429
- BioCyc:   TTHE262724:TT_C1563-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR004521
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027
- TIGRFAMs:   TIGR00451

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39891; Mature: 39891

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGLSAKRLVVKVGSAVLTGERGLDLEAMAEIARQVAALREEGREVVLVSSGAVAAGMR
CCCCCCHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHH
RLGLKERPKDMPKKQALAALGQPLLMAFWQEAFAPFGLPVAQVLLTAEDLSSRSRYLNAK
HCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATLRALLDLGAIPVINENDTVAFEEIRFGDNDQLSARVAALVEAGLLALLSDVDALYEED
HHHHHHHHHCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKKNPQARPIPEVESVEAVLAHAGEENPLGSGGMKSKLLAARIAGRVGIPTLLLPGKRPG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCC
VLLQALSGAPLGTYFHARRRYRGEKAWLFGLLRPKGELVLDRGAVRALKERGASLLPAGV
HHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEECHHHHHHHHHCCCCCCCCCH
KEVRGRFSRGEAVRLLSEEGEEVGVGLANYASEEIARIKGRRSAEIEAVLGYRYTEEVVH
HHHHCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHH
RDHLALKEEA
HHHHHHCCCC
>Mature Secondary Structure
MRPGLSAKRLVVKVGSAVLTGERGLDLEAMAEIARQVAALREEGREVVLVSSGAVAAGMR
CCCCCCHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHH
RLGLKERPKDMPKKQALAALGQPLLMAFWQEAFAPFGLPVAQVLLTAEDLSSRSRYLNAK
HCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATLRALLDLGAIPVINENDTVAFEEIRFGDNDQLSARVAALVEAGLLALLSDVDALYEED
HHHHHHHHHCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKKNPQARPIPEVESVEAVLAHAGEENPLGSGGMKSKLLAARIAGRVGIPTLLLPGKRPG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCC
VLLQALSGAPLGTYFHARRRYRGEKAWLFGLLRPKGELVLDRGAVRALKERGASLLPAGV
HHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEECHHHHHHHHHCCCCCCCCCH
KEVRGRFSRGEAVRLLSEEGEEVGVGLANYASEEIARIKGRRSAEIEAVLGYRYTEEVVH
HHHHCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHH
RDHLALKEEA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7988899