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The map label for this gene is ycdT [H]

Identifier: 55981727

GI number: 55981727

Start: 1646104

End: 1647282

Strand: Direct

Name: ycdT [H]

Synonym: TTHA1758

Alternate gene names: 55981727

Gene position: 1646104-1647282 (Clockwise)

Preceding gene: 55981726

Following gene: 55981741

Centisome position: 88.99

GC content: 70.82

Gene sequence:

>1179_bases
GTGGTCCTCCCCTGGCTGGCCCTGCCCCTCGGGGGGCTTTTCCTCCTGCCCGCGGTGTTGGCCAAGGACCCGGTGCCCTT
CGCCCTGCGCCTCCTCGCCCTCTACGGGGGGCTTGCGGGGGCCTTGGGCCTCCTCTACGCCCTCCACCTGGAGTGGCGCC
GCACCGAGGAACGGGGCCGCCGCTGGACCCGGTTCCTGGAGGCCCTGGCCCGGCTTTCCCCCCGGGTGACCCAGACCCTA
AGCCCCGAGGGGGTTCTCCTCGGGGTCCTCGAGGCCGCCCAGGAGCTCCTCCCCCAGGCGGTGGGCCTGGAGCTCAGGGC
CCGGGGGAGCCTGGTGGGGGAGCGCGCCCCCCACCTCCTTCCCGTCCCCCTGAACGGGGACGCCGCCTACCTCCACCTAA
GGACCCCGCCGGAGGAGCCCGTTCCGCCGGGGTTTCTCGCCCTCCTGGGGGAACGCCTGCGCCAGGTCCTGAGGCAGGCG
GAGTGGGGCGCCATGGCCTACACCGACCCCCTCACGGGGCTTTTCAACCGCCGGGGCCTCGAGGTGGAGCTTCCCAAGCT
CCTCGCCCTCTCCCGGCGCTACCTGGCCCCGGTGAGCGTGGTCATGCTGGACATAGACCGCTTCAAGGCAGTGAACGACA
CCTACGGCCACCCCGTGGGGGACGAGGTGCTCAGGCGCCTGGGGCGGATCCTCAAGGAAAGCGTCCGCAAGGAGGACCTG
GCGGTGCGCTACGGGGGCGAGGAGTTCCTCCTGGTGCTCTTCGGCGCCGAGAGGGAGGCGGCCAAGGAGGTGGTGGAGCG
GATCCGCGAACGCTTCCGGAACGAACGGGTGGCCCCCATCCCCTACCCCCTCACCCTCTCCGCCGGGATCGCCGGGGGGG
AGGTGCCGGAGGGGAGGGAGAGAGACCCTAGAAGAGTGGATCCTCAAGGCCGACTACGCCCTCCTCCGGGCCAAGGAGAC
GGGGCGGGACCGGGTGACCCTGGCCTGAGTACCCCGCTCTGGCTTTCGCCAGAGCGGGGGCCCCGAGGAAGGGCTACTTC
TTGGGCATCATCTTGGCCATCATGGCCTCGAGGGTCTTCCGCTCCTCGTTCATGTGGCCGAAGGCCTTGCCCAAGGCCTT
CAGGAAAAGGAAGAGCACCCCCAGGGCCTTCTGGACCTCGGGGTCGGAAAGCTGCTTGA

Upstream 100 bases:

>100_bases
TGGCGGAGTTTCTCAAAGGGATTGAGGTGCTGAGGGGGGCCCTCGAGGCCCTGGCGCAAACGCACGCGGGGGAGAAGGAG
GGGTAGGCTAAGCTAAACGC

Downstream 100 bases:

>100_bases
AGAACCCGGCGAGGCCCACCCGGGGCGGGTCCTGCATCGCCTCCGGGGTGAAGGTCTCCGCGAGGCCCTTTTGCAGGGCC
CCCGCCATCATGCCCACCGC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MVLPWLALPLGGLFLLPAVLAKDPVPFALRLLALYGGLAGALGLLYALHLEWRRTEERGRRWTRFLEALARLSPRVTQTL
SPEGVLLGVLEAAQELLPQAVGLELRARGSLVGERAPHLLPVPLNGDAAYLHLRTPPEEPVPPGFLALLGERLRQVLRQA
EWGAMAYTDPLTGLFNRRGLEVELPKLLALSRRYLAPVSVVMLDIDRFKAVNDTYGHPVGDEVLRRLGRILKESVRKEDL
AVRYGGEEFLLVLFGAEREAAKEVVERIRERFRNERVAPIPYPLTLSAGIAGGEVPEGRERDPRRVDPQGRLRPPPGQGD
GAGPGDPGLSTPLWLSPERGPRGRATSWASSWPSWPRGSSAPRSCGRRPCPRPSGKGRAPPGPSGPRGRKAA

Sequences:

>Translated_392_residues
MVLPWLALPLGGLFLLPAVLAKDPVPFALRLLALYGGLAGALGLLYALHLEWRRTEERGRRWTRFLEALARLSPRVTQTL
SPEGVLLGVLEAAQELLPQAVGLELRARGSLVGERAPHLLPVPLNGDAAYLHLRTPPEEPVPPGFLALLGERLRQVLRQA
EWGAMAYTDPLTGLFNRRGLEVELPKLLALSRRYLAPVSVVMLDIDRFKAVNDTYGHPVGDEVLRRLGRILKESVRKEDL
AVRYGGEEFLLVLFGAEREAAKEVVERIRERFRNERVAPIPYPLTLSAGIAGGEVPEGRERDPRRVDPQGRLRPPPGQGD
GAGPGDPGLSTPLWLSPERGPRGRATSWASSWPSWPRGSSAPRSCGRRPCPRPSGKGRAPPGPSGPRGRKAA
>Mature_392_residues
MVLPWLALPLGGLFLLPAVLAKDPVPFALRLLALYGGLAGALGLLYALHLEWRRTEERGRRWTRFLEALARLSPRVTQTL
SPEGVLLGVLEAAQELLPQAVGLELRARGSLVGERAPHLLPVPLNGDAAYLHLRTPPEEPVPPGFLALLGERLRQVLRQA
EWGAMAYTDPLTGLFNRRGLEVELPKLLALSRRYLAPVSVVMLDIDRFKAVNDTYGHPVGDEVLRRLGRILKESVRKEDL
AVRYGGEEFLLVLFGAEREAAKEVVERIRERFRNERVAPIPYPLTLSAGIAGGEVPEGRERDPRRVDPQGRLRPPPGQGD
GAGPGDPGLSTPLWLSPERGPRGRATSWASSWPSWPRGSSAPRSCGRRPCPRPSGKGRAPPGPSGPRGRKAA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=131, Percent_Identity=42.7480916030534, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI145693134, Length=154, Percent_Identity=35.7142857142857, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=129, Percent_Identity=35.6589147286822, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=161, Percent_Identity=37.888198757764, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=133, Percent_Identity=39.8496240601504, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081974, Length=123, Percent_Identity=35.7723577235772, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=127, Percent_Identity=36.2204724409449, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=115, Percent_Identity=39.1304347826087, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=127, Percent_Identity=38.5826771653543, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787541, Length=128, Percent_Identity=34.375, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=30.6748466257669, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787802, Length=123, Percent_Identity=34.1463414634146, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=32.0987654320988, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42896; Mature: 42896

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPWLALPLGGLFLLPAVLAKDPVPFALRLLALYGGLAGALGLLYALHLEWRRTEERGR
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWTRFLEALARLSPRVTQTLSPEGVLLGVLEAAQELLPQAVGLELRARGSLVGERAPHLL
HHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCEE
PVPLNGDAAYLHLRTPPEEPVPPGFLALLGERLRQVLRQAEWGAMAYTDPLTGLFNRRGL
EEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCC
EVELPKLLALSRRYLAPVSVVMLDIDRFKAVNDTYGHPVGDEVLRRLGRILKESVRKEDL
CEEHHHHHHHHHHHHCHHHHHEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRYGGEEFLLVLFGAEREAAKEVVERIRERFRNERVAPIPYPLTLSAGIAGGEVPEGRE
EEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCC
RDPRRVDPQGRLRPPPGQGDGAGPGDPGLSTPLWLSPERGPRGRATSWASSWPSWPRGSS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCC
APRSCGRRPCPRPSGKGRAPPGPSGPRGRKAA
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVLPWLALPLGGLFLLPAVLAKDPVPFALRLLALYGGLAGALGLLYALHLEWRRTEERGR
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWTRFLEALARLSPRVTQTLSPEGVLLGVLEAAQELLPQAVGLELRARGSLVGERAPHLL
HHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCEE
PVPLNGDAAYLHLRTPPEEPVPPGFLALLGERLRQVLRQAEWGAMAYTDPLTGLFNRRGL
EEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHCCCC
EVELPKLLALSRRYLAPVSVVMLDIDRFKAVNDTYGHPVGDEVLRRLGRILKESVRKEDL
CEEHHHHHHHHHHHHCHHHHHEEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRYGGEEFLLVLFGAEREAAKEVVERIRERFRNERVAPIPYPLTLSAGIAGGEVPEGRE
EEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCC
RDPRRVDPQGRLRPPPGQGDGAGPGDPGLSTPLWLSPERGPRGRATSWASSWPSWPRGSS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCCC
APRSCGRRPCPRPSGKGRAPPGPSGPRGRKAA
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]