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The map label for this gene is pnp [H]

Identifier: 55981717

GI number: 55981717

Start: 1637275

End: 1637652

Strand: Reverse

Name: pnp [H]

Synonym: TTHA1748

Alternate gene names: 55981717

Gene position: 1637652-1637275 (Counterclockwise)

Preceding gene: 55981718

Following gene: 55981716

Centisome position: 88.53

GC content: 65.34

Gene sequence:

>378_bases
GTGGAACTAGAAGCAGGAACCGTTGTGGAAGGCCGCGTGGTGCGGGTGGTGAGCTTTGGCGCGTTTGTGGAGCTTGCGGG
GGGGGAGCAGGGCCTGGTCCACATCTCCCAGATCGCCCACGAGTACGTGACGAACGTTCGGGACTACCTCAACGAGGGGG
ACATCGTCCAGGTCCTGATTAAAGGGCGGGACGCCAAGGGGCGGCTGGACCTCTCCATCAAGGACCTGACCCCGGCCCCC
GAAGGGGGCGCGCCCCCGCCCAGGCCCAGGCGGCTTCCCAAGCAATCCCCCGAGTTTGAGAACAAGCTCAAGAGCTTCCT
CCGGGGCACCGGAGGGTTTGGGGGCAAGGGCAAAAAGGGCGGCCGGAAGAAGCGCTAA

Upstream 100 bases:

>100_bases
ACCTTCGCCGAGCTCCTCGCGGGGCTGGACCCTTCTGGGGTCCGGCCCTATGTGGTATAGTGGGGGCACCGCGGGTGGAT
TCCGCGCGAGGAGAAGGAAC

Downstream 100 bases:

>100_bases
AGGCCGAGGAAGGCGCCCCCGGGGCGTTGTCGTCCCCGGGGGTTTGCCGTAGAGTAGGGGGGTATGGCGAAGCCCATTGA
GGTGACCGACCAGAACTTTG

Product: hypothetical protein

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MELEAGTVVEGRVVRVVSFGAFVELAGGEQGLVHISQIAHEYVTNVRDYLNEGDIVQVLIKGRDAKGRLDLSIKDLTPAP
EGGAPPPRPRRLPKQSPEFENKLKSFLRGTGGFGGKGKKGGRKKR

Sequences:

>Translated_125_residues
MELEAGTVVEGRVVRVVSFGAFVELAGGEQGLVHISQIAHEYVTNVRDYLNEGDIVQVLIKGRDAKGRLDLSIKDLTPAP
EGGAPPPRPRRLPKQSPEFENKLKSFLRGTGGFGGKGKKGGRKKR
>Mature_125_residues
MELEAGTVVEGRVVRVVSFGAFVELAGGEQGLVHISQIAHEYVTNVRDYLNEGDIVQVLIKGRDAKGRLDLSIKDLTPAP
EGGAPPPRPRRLPKQSPEFENKLKSFLRGTGGFGGKGKKGGRKKR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1098

COG function: function code J; Predicted RNA binding protein (contains ribosomal protein S1 domain)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI145693187, Length=76, Percent_Identity=52.6315789473684, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 13534; Mature: 13534

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEAGTVVEGRVVRVVSFGAFVELAGGEQGLVHISQIAHEYVTNVRDYLNEGDIVQVLI
CCCCCCCEECCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KGRDAKGRLDLSIKDLTPAPEGGAPPPRPRRLPKQSPEFENKLKSFLRGTGGFGGKGKKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GRKKR
CCCCC
>Mature Secondary Structure
MELEAGTVVEGRVVRVVSFGAFVELAGGEQGLVHISQIAHEYVTNVRDYLNEGDIVQVLI
CCCCCCCEECCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KGRDAKGRLDLSIKDLTPAPEGGAPPPRPRRLPKQSPEFENKLKSFLRGTGGFGGKGKKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GRKKR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA