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The map label for this gene is appA [H]

Identifier: 55981603

GI number: 55981603

Start: 1547987

End: 1549855

Strand: Reverse

Name: appA [H]

Synonym: TTHA1634

Alternate gene names: 55981603

Gene position: 1549855-1547987 (Counterclockwise)

Preceding gene: 55981604

Following gene: 55981602

Centisome position: 83.79

GC content: 64.31

Gene sequence:

>1869_bases
ATGAGGAACCTAGGTAAGCTGGCTGTACTCGGTATAACCGCTTTGGGCCTGGCCCTGGCGGGGCCCCAGGACAACAGCCT
CGTCATCGGGGCCTCCCAGGAGCCCAGGGTCTTGGCGGGGGACTTCCTCCGCGTCATCTCCAACCAGGCCATCAAGTCGG
AGATTGAGCAGTACCTCTTCGCCCCCTTCATCGGCTTCAACGCCGACAGCCAGAACTTCCCCGTTTTGGCCACCGAGGTG
CCCACCTTGGAGAACGGCCGCCTGCGGGTCACGGACATCGGCGGGGGCAAGAAGCGCCTGGAGATGGACATCACCATCCG
CCCCGACGCCAAGTGGTCCGACGGCAGGCCCATCACCACGGAGGACGTGGCCTTCTACTTTGAGGTGGGGAAGGCCAAGG
GCATGCCGGTGCTCAACCCCGACTTCTGGGAGCGGGTGAACGTCCGCATCAAGGACGCCCGCAACTTCACCCTCATCTTT
GAGCCTGCCTACTACTACGACACTTACGGCCCCATTAACACCTATGCCCCCAAGCACATCATGGGGCCCGAGTGGGAGCG
GGTGAAGGCGGCGGCCCGGGGCCTGGACCCCGACAAGGACGCGGAGAAGCTCAACGAGCTCTACCGCAACTTCTTCCTCA
AGTTCGCCACCCCCCAGGCGCTGAACCGCGGGGCCATGGTCTACTCCGGGCCCTTCAAGCTCAAGCGCTGGGTGCCGGGG
AACTCCATTGAGATGGAGCGGAACCCCAACTTCCCCATCAAGCCTGAGGGCGGCGAGAGCAAGTACGTGCAGAAGGTGGT
CTACCGCTTCATCCAGAACACCAACTCCCTCCTGGTGGCGGTGATCGGCGGCTCCATTGACGCCACCTCCAGCGTCTCCC
TCACCTTTGACCAGGGCCGCTCGCCCCAGCTGGTGCGGCGCGCTCCCGGCCGCTTTGACATCTGGTTCGTCCCCGGCGCC
ATCTGGGAGCACATTGACATCAACAAGTTTGAGAACTGCCAGGTGGTCAAGGACCTCGGTCTTAACGACAAGCGCACCCG
CCAGGCCATCCTCCACGCCCTGAACCGGGAGGGCCTGGTGAAGGCCTTCTTTGACGGCCTCCAGCCCGTGGCCCACACCT
GGATCGCCCCCGTCAACCCCCTCTTCAACCCCAACGTGAAGAAGTACGAGTTTGACCTGAAGAAGGCGGAGGCCCTCCTG
GCGGAGATGGGCTGGAGGAAGGGGCCGGACGGCATCCTCCAGCGCACCGTGAACGGGCGCACCGTGCGCTTTGAGATTGA
GTACGTCACCACCGCGGGCAACGTCGTCCGCGAGCGCACCCAGCAGTTCTTCGCCGAGGACCTCAAGAAGATCGGCATCG
CCGTCAAGATCAACAACGCCCCCAGCGCCGTGGTCTTCGCCGACGAGTTCATCCAGCGGGCCAGCGAGTGCAAGTGGACG
GGGATGTTTGAGTTCGCCTGGGTCTCCAACCTGCAGGAGGACGGCTCCCTCTTCCAGTACAAGAACCTGAACACCGGGGC
CATCATGGTCCCCACCAAGGAGAACAACTACCAGGGCCAGAACATCGGCGGCTGGCGGAACGACGAGTTTGACCGCCTCA
CGAGCCAGGCCGTGCTGGAGTTTGACCCCGAGCGGCGCAAGCAGCTCTTCTGGCGCGCCCAGGAGATCTGGGCCGAGGAG
CTCCCGGCGCTTCCCCTCTACTTCCGCGCCAACCCCTACGTGGTGCGGAAGGGCCTCGTGAACTACGTGGCCAGCGCCTA
CTCCGGCGGCTACGGCTACCCCGGCTGGAACGCCTGGGAGATCGGCTGGGAGAGCCGGGGCGCCGTGAAGAAGTGGGACC
AGGCGAAGTACGCCCTTTCCACCAGGTAA

Upstream 100 bases:

>100_bases
CCTGGCTTCAGGCCCCTTTAGCTTTGGGCCTTATTGACCTTCATCGGAGGGGCCGTATAATAACCTCGGCCACTGCGATA
GTGCCACGAAAGGAGGCGGT

Downstream 100 bases:

>100_bases
CCCGGGTGGTATAGTGAGGCCCGCTGGGCTTTAGCCCAGCGGGCTTTTTGGTGGAACAAACGGGAGGTACAAGGGTGTTC
GCCTACACGGTGCGCAGGCT

Product: peptide ABC transporter peptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MRNLGKLAVLGITALGLALAGPQDNSLVIGASQEPRVLAGDFLRVISNQAIKSEIEQYLFAPFIGFNADSQNFPVLATEV
PTLENGRLRVTDIGGGKKRLEMDITIRPDAKWSDGRPITTEDVAFYFEVGKAKGMPVLNPDFWERVNVRIKDARNFTLIF
EPAYYYDTYGPINTYAPKHIMGPEWERVKAAARGLDPDKDAEKLNELYRNFFLKFATPQALNRGAMVYSGPFKLKRWVPG
NSIEMERNPNFPIKPEGGESKYVQKVVYRFIQNTNSLLVAVIGGSIDATSSVSLTFDQGRSPQLVRRAPGRFDIWFVPGA
IWEHIDINKFENCQVVKDLGLNDKRTRQAILHALNREGLVKAFFDGLQPVAHTWIAPVNPLFNPNVKKYEFDLKKAEALL
AEMGWRKGPDGILQRTVNGRTVRFEIEYVTTAGNVVRERTQQFFAEDLKKIGIAVKINNAPSAVVFADEFIQRASECKWT
GMFEFAWVSNLQEDGSLFQYKNLNTGAIMVPTKENNYQGQNIGGWRNDEFDRLTSQAVLEFDPERRKQLFWRAQEIWAEE
LPALPLYFRANPYVVRKGLVNYVASAYSGGYGYPGWNAWEIGWESRGAVKKWDQAKYALSTR

Sequences:

>Translated_622_residues
MRNLGKLAVLGITALGLALAGPQDNSLVIGASQEPRVLAGDFLRVISNQAIKSEIEQYLFAPFIGFNADSQNFPVLATEV
PTLENGRLRVTDIGGGKKRLEMDITIRPDAKWSDGRPITTEDVAFYFEVGKAKGMPVLNPDFWERVNVRIKDARNFTLIF
EPAYYYDTYGPINTYAPKHIMGPEWERVKAAARGLDPDKDAEKLNELYRNFFLKFATPQALNRGAMVYSGPFKLKRWVPG
NSIEMERNPNFPIKPEGGESKYVQKVVYRFIQNTNSLLVAVIGGSIDATSSVSLTFDQGRSPQLVRRAPGRFDIWFVPGA
IWEHIDINKFENCQVVKDLGLNDKRTRQAILHALNREGLVKAFFDGLQPVAHTWIAPVNPLFNPNVKKYEFDLKKAEALL
AEMGWRKGPDGILQRTVNGRTVRFEIEYVTTAGNVVRERTQQFFAEDLKKIGIAVKINNAPSAVVFADEFIQRASECKWT
GMFEFAWVSNLQEDGSLFQYKNLNTGAIMVPTKENNYQGQNIGGWRNDEFDRLTSQAVLEFDPERRKQLFWRAQEIWAEE
LPALPLYFRANPYVVRKGLVNYVASAYSGGYGYPGWNAWEIGWESRGAVKKWDQAKYALSTR
>Mature_622_residues
MRNLGKLAVLGITALGLALAGPQDNSLVIGASQEPRVLAGDFLRVISNQAIKSEIEQYLFAPFIGFNADSQNFPVLATEV
PTLENGRLRVTDIGGGKKRLEMDITIRPDAKWSDGRPITTEDVAFYFEVGKAKGMPVLNPDFWERVNVRIKDARNFTLIF
EPAYYYDTYGPINTYAPKHIMGPEWERVKAAARGLDPDKDAEKLNELYRNFFLKFATPQALNRGAMVYSGPFKLKRWVPG
NSIEMERNPNFPIKPEGGESKYVQKVVYRFIQNTNSLLVAVIGGSIDATSSVSLTFDQGRSPQLVRRAPGRFDIWFVPGA
IWEHIDINKFENCQVVKDLGLNDKRTRQAILHALNREGLVKAFFDGLQPVAHTWIAPVNPLFNPNVKKYEFDLKKAEALL
AEMGWRKGPDGILQRTVNGRTVRFEIEYVTTAGNVVRERTQQFFAEDLKKIGIAVKINNAPSAVVFADEFIQRASECKWT
GMFEFAWVSNLQEDGSLFQYKNLNTGAIMVPTKENNYQGQNIGGWRNDEFDRLTSQAVLEFDPERRKQLFWRAQEIWAEE
LPALPLYFRANPYVVRKGLVNYVASAYSGGYGYPGWNAWEIGWESRGAVKKWDQAKYALSTR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=248, Percent_Identity=25.4032258064516, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 70471; Mature: 70471

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLGKLAVLGITALGLALAGPQDNSLVIGASQEPRVLAGDFLRVISNQAIKSEIEQYLF
CCCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
APFIGFNADSQNFPVLATEVPTLENGRLRVTDIGGGKKRLEMDITIRPDAKWSDGRPITT
HHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCH
EDVAFYFEVGKAKGMPVLNPDFWERVNVRIKDARNFTLIFEPAYYYDTYGPINTYAPKHI
HHEEEEEEECCCCCCCCCCCCHHHHCCEEEECCCCEEEEECCCEEECCCCCCCCCCCCCC
MGPEWERVKAAARGLDPDKDAEKLNELYRNFFLKFATPQALNRGAMVYSGPFKLKRWVPG
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCEEEEECCC
NSIEMERNPNFPIKPEGGESKYVQKVVYRFIQNTNSLLVAVIGGSIDATSSVSLTFDQGR
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCC
SPQLVRRAPGRFDIWFVPGAIWEHIDINKFENCQVVKDLGLNDKRTRQAILHALNREGLV
CCHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHH
KAFFDGLQPVAHTWIAPVNPLFNPNVKKYEFDLKKAEALLAEMGWRKGPDGILQRTVNGR
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCE
TVRFEIEYVTTAGNVVRERTQQFFAEDLKKIGIAVKINNAPSAVVFADEFIQRASECKWT
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEHHHHHHHHHCCCCC
GMFEFAWVSNLQEDGSLFQYKNLNTGAIMVPTKENNYQGQNIGGWRNDEFDRLTSQAVLE
CEEEHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEE
FDPERRKQLFWRAQEIWAEELPALPLYFRANPYVVRKGLVNYVASAYSGGYGYPGWNAWE
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
IGWESRGAVKKWDQAKYALSTR
ECCCCCCCCHHHHHHCEEECCC
>Mature Secondary Structure
MRNLGKLAVLGITALGLALAGPQDNSLVIGASQEPRVLAGDFLRVISNQAIKSEIEQYLF
CCCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
APFIGFNADSQNFPVLATEVPTLENGRLRVTDIGGGKKRLEMDITIRPDAKWSDGRPITT
HHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCH
EDVAFYFEVGKAKGMPVLNPDFWERVNVRIKDARNFTLIFEPAYYYDTYGPINTYAPKHI
HHEEEEEEECCCCCCCCCCCCHHHHCCEEEECCCCEEEEECCCEEECCCCCCCCCCCCCC
MGPEWERVKAAARGLDPDKDAEKLNELYRNFFLKFATPQALNRGAMVYSGPFKLKRWVPG
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCEEEEECCC
NSIEMERNPNFPIKPEGGESKYVQKVVYRFIQNTNSLLVAVIGGSIDATSSVSLTFDQGR
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCC
SPQLVRRAPGRFDIWFVPGAIWEHIDINKFENCQVVKDLGLNDKRTRQAILHALNREGLV
CCHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHH
KAFFDGLQPVAHTWIAPVNPLFNPNVKKYEFDLKKAEALLAEMGWRKGPDGILQRTVNGR
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCE
TVRFEIEYVTTAGNVVRERTQQFFAEDLKKIGIAVKINNAPSAVVFADEFIQRASECKWT
EEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEHHHHHHHHHCCCCC
GMFEFAWVSNLQEDGSLFQYKNLNTGAIMVPTKENNYQGQNIGGWRNDEFDRLTSQAVLE
CEEEHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEE
FDPERRKQLFWRAQEIWAEELPALPLYFRANPYVVRKGLVNYVASAYSGGYGYPGWNAWE
CCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
IGWESRGAVKKWDQAKYALSTR
ECCCCCCCCHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]