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The map label for this gene is truA

Identifier: 55981600

GI number: 55981600

Start: 1544908

End: 1545669

Strand: Direct

Name: truA

Synonym: TTHA1631

Alternate gene names: 55981600

Gene position: 1544908-1545669 (Clockwise)

Preceding gene: 55981599

Following gene: 55981605

Centisome position: 83.52

GC content: 72.7

Gene sequence:

>762_bases
GTGCGGCGCCTCCTCCTCCTTTGCGAGTACGACGGCACCCTCTTCGCCGGGCTGCAGCGGCAGGGGAGGGGCTTGCGCAC
GGTGCAGGGGGAGCTGGAAAGGGCCCTGCCCGGGATCGGGGCCTTGCCCAAGGCGGTGGCCGCGGGGCGCACGGACGCCG
GGGTGCACGCCCTGGCCATGCCCTTCCACGTGGACGTGGAAAGCGCCATCCCCGTGGAGAAGGTCCCCGAGGCCCTCAAC
CGCCTCCTCCCCGAGGACCTCAAGGTGGTGGGGGCGAGGGAGGTGGCCCCCGACTTCCACGCCCGCAAGGACGCCCTGTG
GCGGGCTTACCGCTACCGGATCCTCGTGAGGCCCCACCCCTCCCCCCTCCTCCGCCACCGGGCCCTTTGGGTGCGGCGCC
CCCTGGACCTGGAGGCCATGGAAGAGGCCCTCTCCCTCCTCCTTGGGCGGCACAACTTCCTGGGCTTCGCCAAGGAGGAA
ACCCGGCCGGGGGAGCGGGAGCTCCTCGAGGCCCGGCTCCAGGTGGCGGAGGGGGAGGCGGGCCTCGAGGTGCGCCTCTA
CTTCCGGGGGAAAAGCTTCCTCCGGGGCCAGGTCCGGGGCATGGTGGGCACCCTCCTGGAGGTGGGGCTTGGGAAGCGGC
CCCCGGAGAGCCTAAAGGCCATCCTGAAAACCGCCGACCGCCGCCTGGCGGGCCCCACCGCCCCGGCCCACGGCCTTTAC
TTCGTGGAGGCCGCCTACCCCGAGGAGAAGCTCAGCCCCTAA

Upstream 100 bases:

>100_bases
CCCTTCAAGGTGGGGGACCGGGTAGGGCTAGAGGTGGTGGGGGAAGGGGTGGCCCTGGAGGGTACCCCCCCTGCCCCGGT
CCGGCAGGAGGCCTGAGGGC

Downstream 100 bases:

>100_bases
ACCCCCATGCCGGCGCAAGCCGGCATGGGACGGCCTTAGAGGCGGCTCCTCGGGTCCAAAGCGTCCCTAAGCCCGTCCCC
GAGGTAGTTGAAGGCCAGGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRRLLLLCEYDGTLFAGLQRQGRGLRTVQGELERALPGIGALPKAVAAGRTDAGVHALAMPFHVDVESAIPVEKVPEALN
RLLPEDLKVVGAREVAPDFHARKDALWRAYRYRILVRPHPSPLLRHRALWVRRPLDLEAMEEALSLLLGRHNFLGFAKEE
TRPGERELLEARLQVAEGEAGLEVRLYFRGKSFLRGQVRGMVGTLLEVGLGKRPPESLKAILKTADRRLAGPTAPAHGLY
FVEAAYPEEKLSP

Sequences:

>Translated_253_residues
MRRLLLLCEYDGTLFAGLQRQGRGLRTVQGELERALPGIGALPKAVAAGRTDAGVHALAMPFHVDVESAIPVEKVPEALN
RLLPEDLKVVGAREVAPDFHARKDALWRAYRYRILVRPHPSPLLRHRALWVRRPLDLEAMEEALSLLLGRHNFLGFAKEE
TRPGERELLEARLQVAEGEAGLEVRLYFRGKSFLRGQVRGMVGTLLEVGLGKRPPESLKAILKTADRRLAGPTAPAHGLY
FVEAAYPEEKLSP
>Mature_253_residues
MRRLLLLCEYDGTLFAGLQRQGRGLRTVQGELERALPGIGALPKAVAAGRTDAGVHALAMPFHVDVESAIPVEKVPEALN
RLLPEDLKVVGAREVAPDFHARKDALWRAYRYRILVRPHPSPLLRHRALWVRRPLDLEAMEEALSLLLGRHNFLGFAKEE
TRPGERELLEARLQVAEGEAGLEVRLYFRGKSFLRGQVRGMVGTLLEVGLGKRPPESLKAILKTADRRLAGPTAPAHGLY
FVEAAYPEEKLSP

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=34.0659340659341, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI199559785, Length=274, Percent_Identity=24.8175182481752, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=34.136546184739, Blast_Score=125, Evalue=4e-30,
Organism=Drosophila melanogaster, GI116007752, Length=310, Percent_Identity=30, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_THET2 (Q72I65)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005236.1
- ProteinModelPortal:   Q72I65
- SMR:   Q72I65
- STRING:   Q72I65
- GeneID:   2774664
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q72I65
- ProtClustDB:   PRK00021
- BioCyc:   TTHE262724:TT_C1267-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28134; Mature: 28134

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLLLCEYDGTLFAGLQRQGRGLRTVQGELERALPGIGALPKAVAAGRTDAGVHALAM
CCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEE
PFHVDVESAIPVEKVPEALNRLLPEDLKVVGAREVAPDFHARKDALWRAYRYRILVRPHP
EEEECCHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCC
SPLLRHRALWVRRPLDLEAMEEALSLLLGRHNFLGFAKEETRPGERELLEARLQVAEGEA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
GLEVRLYFRGKSFLRGQVRGMVGTLLEVGLGKRPPESLKAILKTADRRLAGPTAPAHGLY
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
FVEAAYPEEKLSP
EEEECCCHHCCCC
>Mature Secondary Structure
MRRLLLLCEYDGTLFAGLQRQGRGLRTVQGELERALPGIGALPKAVAAGRTDAGVHALAM
CCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEE
PFHVDVESAIPVEKVPEALNRLLPEDLKVVGAREVAPDFHARKDALWRAYRYRILVRPHP
EEEECCHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCC
SPLLRHRALWVRRPLDLEAMEEALSLLLGRHNFLGFAKEETRPGERELLEARLQVAEGEA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
GLEVRLYFRGKSFLRGQVRGMVGTLLEVGLGKRPPESLKAILKTADRRLAGPTAPAHGLY
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
FVEAAYPEEKLSP
EEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA