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The map label for this gene is ogt [H]

Identifier: 55981533

GI number: 55981533

Start: 1487635

End: 1488078

Strand: Reverse

Name: ogt [H]

Synonym: TTHA1564

Alternate gene names: 55981533

Gene position: 1488078-1487635 (Counterclockwise)

Preceding gene: 55981534

Following gene: 55981531

Centisome position: 80.45

GC content: 73.42

Gene sequence:

>444_bases
GTGTGGCTCCCCACGCCCTTAGGTCCCCTGTGGCTGGAGGTCTCGCCCCTGGGGGTGCGCCGCCTCGAGCCCGCCCTCTA
CCCCCGGGGTCCGGAGGCCGAAGGGGCGCTGGCCCTTAGGGTGCGGGAGGCGGTCCAGGCCTACTTCGCCGGGGAGCGGC
CCGACTTCCTGGACCTCCCCCTGGACTACACCGGCCTCTCCCCGGCCAGGCTCCGCCTCTACGAGCGGGTCCGCCTTGTG
CCCTACGGGCGGACCGTGAGCTACGGGGCCTTGGGCCGGGAGCTCGGCCTCTCCCCCAGGGCGGTGGGGGCGGCCCTTAG
GGCCTGCCCCTTTTTCCTCCTCGTCCCCGCCCACCGGGTGATCCACGCCGACGGGCGGCTTGGGGGGTTTCAGGGGCAGG
AGGGGCTGAAGCTTTGGCTCCTCCGCTTTGAGGGGGCCCTTTAG

Upstream 100 bases:

>100_bases
AAGGGGATGGCGGAGCGGGGCTACGGCTACCGGGAGATCCTGGGGCACTACTTCCCGGGCACCCTCCTCTCCCCCCTCGA
GGTGGCGGCGCGCTAGGGCC

Downstream 100 bases:

>100_bases
GCCTCCCGCCACAGGGCCAGGGAGAAGGGCGGAAGCACGGGGCCGCAGAGCCTCCCCCCCTGGGGCGTGCAGACCTCCCC
GGAGAGGAGGTCCACCGCCC

Product: methylated-DNA--protein-cysteine methyltransferase (Ogt)

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MWLPTPLGPLWLEVSPLGVRRLEPALYPRGPEAEGALALRVREAVQAYFAGERPDFLDLPLDYTGLSPARLRLYERVRLV
PYGRTVSYGALGRELGLSPRAVGAALRACPFFLLVPAHRVIHADGRLGGFQGQEGLKLWLLRFEGAL

Sequences:

>Translated_147_residues
MWLPTPLGPLWLEVSPLGVRRLEPALYPRGPEAEGALALRVREAVQAYFAGERPDFLDLPLDYTGLSPARLRLYERVRLV
PYGRTVSYGALGRELGLSPRAVGAALRACPFFLLVPAHRVIHADGRLGGFQGQEGLKLWLLRFEGAL
>Mature_147_residues
MWLPTPLGPLWLEVSPLGVRRLEPALYPRGPEAEGALALRVREAVQAYFAGERPDFLDLPLDYTGLSPARLRLYERVRLV
PYGRTVSYGALGRELGLSPRAVGAALRACPFFLLVPAHRVIHADGRLGGFQGQEGLKLWLLRFEGAL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=166, Percent_Identity=34.9397590361446, Blast_Score=73, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16258; Mature: 16258

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLPTPLGPLWLEVSPLGVRRLEPALYPRGPEAEGALALRVREAVQAYFAGERPDFLDLP
CCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECC
LDYTGLSPARLRLYERVRLVPYGRTVSYGALGRELGLSPRAVGAALRACPFFLLVPAHRV
CCCCCCCHHHHHHHHHHEEEECCCEECCCCCHHHHCCCHHHHHHHHHHCCCEEEECCCEE
IHADGRLGGFQGQEGLKLWLLRFEGAL
EECCCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MWLPTPLGPLWLEVSPLGVRRLEPALYPRGPEAEGALALRVREAVQAYFAGERPDFLDLP
CCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECC
LDYTGLSPARLRLYERVRLVPYGRTVSYGALGRELGLSPRAVGAALRACPFFLLVPAHRV
CCCCCCCHHHHHHHHHHEEEECCCEECCCCCHHHHCCCHHHHHHHHHHCCCEEEECCCEE
IHADGRLGGFQGQEGLKLWLLRFEGAL
EECCCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA