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The map label for this gene is amiA [H]

Identifier: 55981341

GI number: 55981341

Start: 1308767

End: 1309849

Strand: Reverse

Name: amiA [H]

Synonym: TTHA1372

Alternate gene names: 55981341

Gene position: 1309849-1308767 (Counterclockwise)

Preceding gene: 55981344

Following gene: 55981338

Centisome position: 70.81

GC content: 70.36

Gene sequence:

>1083_bases
ATGCGCTTCCTTCTCCTCTTCCTCTGGCTTTGGGCCTGGGCTTGGGCCTTTCCCCGGGTGGGGGTCCAGGAGGGCTTCAC
CCGGGTCGTCTTTGACCTGCCCTCGGAGGCGGTGCGCTACACCCTGGAGCGGGGGGAGAACCTCCTCGTGGTGGTGCTCT
TGGGCCTCAAGGCCCCGCCCACGGAGGAGGTGGTGAACTCCAAGGAGGTGGCCTCGGTCCAGACCCTTCCGGAGAAGGAA
GGGGTCCGGGTCCTCATCCGCACCAAGGGGCCGGTGGAGGTGACCGTTTCCCGCTACAAGGACCCCGAGCGCCTGGTCTT
GGACCTGAGCCTCGCGCAAAAGGCCACCGCCCCCCCTCCGCCGCCGAAGCCCAAGCCCCCCGACCCCCCTAAGCCCGTGG
TCCTCCTGGACCCGGGGCACGGCGGGGTGGACCCGGGGATGGTGGGGCACGTGGTGGAAAAGGAGGTGGTCCTGGACGTG
GCCCTCCGCCTCAAGCGCCTCCTGGAGAAGGAGGGGATTGAGGTCCGCCTCACCCGGGACAAGGACATGCACCTCTCCCC
CGACAAGCGGGAGGACCTTTCCCGGCGGGCGGCCATGGCGGACAGCTCCCGGGTGAACCTCTTCATCTCCATCCACGTGA
ACGCCTCCCCCACCCGCACCGCCCAAGGGGTGGAGGCCTACTACTTCGGCCGGGCGCAGGACCCGAGGGTCGTGGCCCAG
GTGATCCGGGAAAACGGCGGCGGGGAGCTGGGCCGGCGCCTCACCGAGGAGGCCAAGAGCGTGGCGGAGCGGATCCTCAC
GGACATCGTGGCCCAGGCGAACCAGCGCTACAGCCAGCGCCTGGCGGAGACCCTGGGCCGGAAGCTCTCCCAGGCCACGG
GAAGCCCCTACCGGGGGAGTTTCCCCGGCGACTTCTTCGTTCTCCGCTACGCCAAGGTGCCGGCGGTCCTGGTGGAGATC
GGCTTCGGGGACCACCCTGCCGAGGGGCGGAGGCTCGCCGAGGCGGCCTACCGGGAACGGGTAGCCCAGGGCCTGGCGGA
GGGGATCCTCGCCTTTCTCGCCCAGGGGGCCTTCGCCCGCTAG

Upstream 100 bases:

>100_bases
GCCCAGGAAGCGGTGCCCCACGATCTGGTGGACCACGACCTTCTTCGTCATGAGCCGGATTTTACCAGGCCCTCATCCGG
ACGGCTAGAATGGGGCGTAG

Downstream 100 bases:

>100_bases
GGCCTGGGGCAGGGCCTCTTGCCGGGTGGGGGGATCTTCCGGAAGGAGGCCGGATACCAGGAGAGGGGAAGGGGCGAGGC
CTTGAGGGTCCAGGGCCTTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRFLLLFLWLWAWAWAFPRVGVQEGFTRVVFDLPSEAVRYTLERGENLLVVVLLGLKAPPTEEVVNSKEVASVQTLPEKE
GVRVLIRTKGPVEVTVSRYKDPERLVLDLSLAQKATAPPPPPKPKPPDPPKPVVLLDPGHGGVDPGMVGHVVEKEVVLDV
ALRLKRLLEKEGIEVRLTRDKDMHLSPDKREDLSRRAAMADSSRVNLFISIHVNASPTRTAQGVEAYYFGRAQDPRVVAQ
VIRENGGGELGRRLTEEAKSVAERILTDIVAQANQRYSQRLAETLGRKLSQATGSPYRGSFPGDFFVLRYAKVPAVLVEI
GFGDHPAEGRRLAEAAYRERVAQGLAEGILAFLAQGAFAR

Sequences:

>Translated_360_residues
MRFLLLFLWLWAWAWAFPRVGVQEGFTRVVFDLPSEAVRYTLERGENLLVVVLLGLKAPPTEEVVNSKEVASVQTLPEKE
GVRVLIRTKGPVEVTVSRYKDPERLVLDLSLAQKATAPPPPPKPKPPDPPKPVVLLDPGHGGVDPGMVGHVVEKEVVLDV
ALRLKRLLEKEGIEVRLTRDKDMHLSPDKREDLSRRAAMADSSRVNLFISIHVNASPTRTAQGVEAYYFGRAQDPRVVAQ
VIRENGGGELGRRLTEEAKSVAERILTDIVAQANQRYSQRLAETLGRKLSQATGSPYRGSFPGDFFVLRYAKVPAVLVEI
GFGDHPAEGRRLAEAAYRERVAQGLAEGILAFLAQGAFAR
>Mature_360_residues
MRFLLLFLWLWAWAWAFPRVGVQEGFTRVVFDLPSEAVRYTLERGENLLVVVLLGLKAPPTEEVVNSKEVASVQTLPEKE
GVRVLIRTKGPVEVTVSRYKDPERLVLDLSLAQKATAPPPPPKPKPPDPPKPVVLLDPGHGGVDPGMVGHVVEKEVVLDV
ALRLKRLLEKEGIEVRLTRDKDMHLSPDKREDLSRRAAMADSSRVNLFISIHVNASPTRTAQGVEAYYFGRAQDPRVVAQ
VIRENGGGELGRRLTEEAKSVAERILTDIVAQANQRYSQRLAETLGRKLSQATGSPYRGSFPGDFFVLRYAKVPAVLVEI
GFGDHPAEGRRLAEAAYRERVAQGLAEGILAFLAQGAFAR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=244, Percent_Identity=30.327868852459, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082163, Length=282, Percent_Identity=29.0780141843972, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790611, Length=232, Percent_Identity=26.2931034482759, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 39799; Mature: 39799

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLLLFLWLWAWAWAFPRVGVQEGFTRVVFDLPSEAVRYTLERGENLLVVVLLGLKAPP
CHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCC
TEEVVNSKEVASVQTLPEKEGVRVLIRTKGPVEVTVSRYKDPERLVLDLSLAQKATAPPP
HHHHHCCHHHHHHHCCCCCCCCEEEEECCCCEEEEHCCCCCHHHEEEEHHHHHHCCCCCC
PPKPKPPDPPKPVVLLDPGHGGVDPGMVGHVVEKEVVLDVALRLKRLLEKEGIEVRLTRD
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
KDMHLSPDKREDLSRRAAMADSSRVNLFISIHVNASPTRTAQGVEAYYFGRAQDPRVVAQ
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHCCCCEEEECCCCCHHHHHH
VIRENGGGELGRRLTEEAKSVAERILTDIVAQANQRYSQRLAETLGRKLSQATGSPYRGS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FPGDFFVLRYAKVPAVLVEIGFGDHPAEGRRLAEAAYRERVAQGLAEGILAFLAQGAFAR
CCCCEEEEEHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRFLLLFLWLWAWAWAFPRVGVQEGFTRVVFDLPSEAVRYTLERGENLLVVVLLGLKAPP
CHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCC
TEEVVNSKEVASVQTLPEKEGVRVLIRTKGPVEVTVSRYKDPERLVLDLSLAQKATAPPP
HHHHHCCHHHHHHHCCCCCCCCEEEEECCCCEEEEHCCCCCHHHEEEEHHHHHHCCCCCC
PPKPKPPDPPKPVVLLDPGHGGVDPGMVGHVVEKEVVLDVALRLKRLLEKEGIEVRLTRD
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
KDMHLSPDKREDLSRRAAMADSSRVNLFISIHVNASPTRTAQGVEAYYFGRAQDPRVVAQ
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHCCCCEEEECCCCCHHHHHH
VIRENGGGELGRRLTEEAKSVAERILTDIVAQANQRYSQRLAETLGRKLSQATGSPYRGS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FPGDFFVLRYAKVPAVLVEIGFGDHPAEGRRLAEAAYRERVAQGLAEGILAFLAQGAFAR
CCCCEEEEEHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]