Click here to switch to the map view.

The map label for this gene is miaB [H]

Identifier: 55981277

GI number: 55981277

Start: 1247254

End: 1248651

Strand: Reverse

Name: miaB [H]

Synonym: TTHA1308

Alternate gene names: 55981277

Gene position: 1248651-1247254 (Counterclockwise)

Preceding gene: 55981279

Following gene: 55981276

Centisome position: 67.5

GC content: 67.67

Gene sequence:

>1398_bases
ATGCCCCCATGCTACCCCTTCCTGCTATCCTGGGAAGGATGGGCCCGGGATCCCCCCGGTCGCCCGGGAGGTGCTATGCG
TGCGCACATCATCACCTACGGCTGCCAGATGAACGAGTACGACTCCCACCTGGTGGCGAGCGAGCTCGTGAGCCTCGGGT
GGGAGCTCGTGGACTCGGTGGAGGAGGCGGACTTCGTCCTGGTGAACACCTGCGCCGTGCGGGGCAAGCCCGTGGAGAAG
GTGCGCTCCCTCCTGGGCCAGCTCCGCAAGGAGAAGGAGCGTCGGGGCCTCCTCATCGGGATGATGGGGTGCCTGGCCCA
GCTGGACGAGGGCCAGCAGATGGCGAAGAAGTTCGGGGTGGACGTCCTCTTGGGCCCCGGGGCCCTCACCTCCTTGCCCG
AGGCCCTGAAGGCGAACGAGAGGTTCTGGGACCTCACCTTCCGAGAGGACGTCCTGGACTACATTCCCCCGCCTCCCAAG
GGGGCGCTTTCCGCCCACGTGACCATCATCCGGGGGTGCAACCACCACTGCACCTACTGCATCGTCCCCACCACCCGCGG
CCCCGAGGTCTCCCGCCACCCCGACCTCATCCTTAAGGAGATTGAGCTCCTGAAGCAGGCGGGGGTGGTGGAGGTCACCC
TCCTCGGCCAGAACGTCAACTCCTACGGCAAGGACCAGCCGGGCTTCCCCTCCTTCGCCGAGCTCCTCCGCATGGTGGGC
GGGATGGGCATCCCCCGGGTGCGCTTCCTCACCAGCCACCCGGTGAACTTCACCGACGACATCATTGAGGCCATCGCCGA
AACCCCCGCCATCTGCCGCTACATCCACCTGCCCGTGCAGTCGGGCTCCGACCGGGTGCTCCGCCGCATGGCCCGGGAGT
ACCGCCGGGCACACTATTTGGAGAGGATCCGGAAGATCCGCGAGGCCCTCCCCGATGCCGTGCTTTCCACCGACATCATC
GTGGGCTTCCCCGGGGAGACGGAGGAGGACTTCCAGGAGACCCTTTCCCTCTACGACGAGGTGGGGTACGACCAGGCCTA
CATGTTCATCTACTCGCCCCGCCCCGGCACCCCCGCCTACAAGCACTTCCAGGACCTGCCCCGGGAGGTGAAGGTGGAGC
GCCTGATGCGCCTCATTGAGAAGCAGAAGGAGTGGAGCTACCGCAGGAACCTGGAGTGGGTGGGGAAGACGGTGGAGGTG
TTGGTCCGGGGCGAGGCCAAGGAGGAGGGCTTCGTCCAGGGGCACGACCGGGGGAACCACCCCGTGCTCGTCCCCGCTTC
CCAGGCCCCGGTGCCGGGCCTCTACCAGGTGGAGATCAAGCAGGCCACGCCCCACCTCCTCTTCGGGGAGGTGGTGGGGG
CGGAGGCCCCGGCCCCCATCCCCCTGCCCGTGGCCTAG

Upstream 100 bases:

>100_bases
CTTGGCCCGCTCTAGGCGCCTTTCCTCCCCGTTCCACCGGAAGGGGGTCCCCTCCGGAAGGTCCAGGACGTAGGGCCCCT
TCTCCCGAAAGCGGAGGCGC

Downstream 100 bases:

>100_bases
GGGGTGAGGGATGCGGTCCATCCTCCCCCTGGGCGTGGCCTTGGCCCTCTTCCTCCTCCTCTTCGCCACCTTCCGCTTCA
CGGGGCTTGGGTTCTTCCTG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPPCYPFLLSWEGWARDPPGRPGGAMRAHIITYGCQMNEYDSHLVASELVSLGWELVDSVEEADFVLVNTCAVRGKPVEK
VRSLLGQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGVDVLLGPGALTSLPEALKANERFWDLTFREDVLDYIPPPPK
GALSAHVTIIRGCNHHCTYCIVPTTRGPEVSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVG
GMGIPRVRFLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYLERIRKIREALPDAVLSTDII
VGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAYKHFQDLPREVKVERLMRLIEKQKEWSYRRNLEWVGKTVEV
LVRGEAKEEGFVQGHDRGNHPVLVPASQAPVPGLYQVEIKQATPHLLFGEVVGAEAPAPIPLPVA

Sequences:

>Translated_465_residues
MPPCYPFLLSWEGWARDPPGRPGGAMRAHIITYGCQMNEYDSHLVASELVSLGWELVDSVEEADFVLVNTCAVRGKPVEK
VRSLLGQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGVDVLLGPGALTSLPEALKANERFWDLTFREDVLDYIPPPPK
GALSAHVTIIRGCNHHCTYCIVPTTRGPEVSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVG
GMGIPRVRFLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYLERIRKIREALPDAVLSTDII
VGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAYKHFQDLPREVKVERLMRLIEKQKEWSYRRNLEWVGKTVEV
LVRGEAKEEGFVQGHDRGNHPVLVPASQAPVPGLYQVEIKQATPHLLFGEVVGAEAPAPIPLPVA
>Mature_464_residues
PPCYPFLLSWEGWARDPPGRPGGAMRAHIITYGCQMNEYDSHLVASELVSLGWELVDSVEEADFVLVNTCAVRGKPVEKV
RSLLGQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGVDVLLGPGALTSLPEALKANERFWDLTFREDVLDYIPPPPKG
ALSAHVTIIRGCNHHCTYCIVPTTRGPEVSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVGG
MGIPRVRFLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYLERIRKIREALPDAVLSTDIIV
GFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAYKHFQDLPREVKVERLMRLIEKQKEWSYRRNLEWVGKTVEVL
VRGEAKEEGFVQGHDRGNHPVLVPASQAPVPGLYQVEIKQATPHLLFGEVVGAEAPAPIPLPVA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=37.3695198329854, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=37.3695198329854, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI93277076, Length=447, Percent_Identity=27.7404921700224, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=43.7641723356009, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=30.5494505494506, Blast_Score=174, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=477, Percent_Identity=33.7526205450734, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=319, Percent_Identity=28.8401253918495, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21356207, Length=451, Percent_Identity=37.2505543237251, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI19922432, Length=448, Percent_Identity=27.0089285714286, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52147; Mature: 52016

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPCYPFLLSWEGWARDPPGRPGGAMRAHIITYGCQMNEYDSHLVASELVSLGWELVDSV
CCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
EEADFVLVNTCAVRGKPVEKVRSLLGQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGV
HHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCC
DVLLGPGALTSLPEALKANERFWDLTFREDVLDYIPPPPKGALSAHVTIIRGCNHHCTYC
EEEECCCHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEE
IVPTTRGPEVSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVG
EEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCHHHHHHHHHHC
GMGIPRVRFLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYL
CCCCCEEEEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ERIRKIREALPDAVLSTDIIVGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAY
HHHHHHHHHCCHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
KHFQDLPREVKVERLMRLIEKQKEWSYRRNLEWVGKTVEVLVRGEAKEEGFVQGHDRGNH
HHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCEECCCCCCC
PVLVPASQAPVPGLYQVEIKQATPHLLFGEVVGAEAPAPIPLPVA
CEEEECCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PPCYPFLLSWEGWARDPPGRPGGAMRAHIITYGCQMNEYDSHLVASELVSLGWELVDSV
CCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
EEADFVLVNTCAVRGKPVEKVRSLLGQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGV
HHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCC
DVLLGPGALTSLPEALKANERFWDLTFREDVLDYIPPPPKGALSAHVTIIRGCNHHCTYC
EEEECCCHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEE
IVPTTRGPEVSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVG
EEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCHHHHHHHHHHC
GMGIPRVRFLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYL
CCCCCEEEEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ERIRKIREALPDAVLSTDIIVGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAY
HHHHHHHHHCCHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHH
KHFQDLPREVKVERLMRLIEKQKEWSYRRNLEWVGKTVEVLVRGEAKEEGFVQGHDRGNH
HHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCEECCCCCCC
PVLVPASQAPVPGLYQVEIKQATPHLLFGEVVGAEAPAPIPLPVA
CEEEECCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA