Click here to switch to the map view.

The map label for this gene is potD [H]

Identifier: 55981206

GI number: 55981206

Start: 1173470

End: 1174267

Strand: Reverse

Name: potD [H]

Synonym: TTHA1237

Alternate gene names: 55981206

Gene position: 1174267-1173470 (Counterclockwise)

Preceding gene: 55981207

Following gene: 55981205

Centisome position: 63.48

GC content: 67.79

Gene sequence:

>798_bases
ATGAGGCGGATTGCGGTGTTGCTCCTGCTCGTCGCGGTGGTGCTCCTCGGGTGGCTTTGGCTTCGGCCCAAGGCGCCTTC
GGGCGCGGGCACCCTTTACTTCCTCAACTGGGCCGACTACATCCCCGAGGAGCTTATCCAGAAGTTTGAGGCGGAGACCG
GGGCCAAGGTGGTCCTGGACACCTTTGAGTCCCCCGAGGCCATGCTGGCGAAGCTCAAGGCGGGGGCGGACCAGGAGTTC
TCCCTGGTGGTGGCCCCCGACTACTACGTCCTGCAGATGGCCCGGGACGGCCTCATCGCCCCCTTGGACAAGGGGAAGCT
GAAGAACCTCGCCAACCTGGACCCCTTCTTCCTAGACCCTCCCTACGACCCCGGCCTGCAGTACTCCGTGCCCTACCTCT
GGGGCACCACGGGGATCGCCTACCGGGAGGACCTGGTGACGGGCCCCGTGGACTCCTACGCCGTCTTCTTTGCTCCGGAG
GCCCAGGTGGGGCCCTTCCTCCTCCTGGACGAGATGCGGGAGACCATAGGAGCGGCCCTGAAGTACCTGGGCTACTCGGT
GAACACCACCGACCCCGAGGCCCTGGCGAAGGCCAAGGAGCTCCTCCTCGCCGCCAAGGAGCGCTCCGTGGGCTTCGCCG
GGGGCCTCGAGGCCCTGAACCGCATCCTGGCGGGGGACGCCGCCTTAAGCCTCGCCTACTCGGGGGACGTCCTCCAGGCC
CGGGAGGAGGACCAGCGCCTCCGCTACACCCTGGCCAAGGAGGGGGGACGCTCTGGACGGACGCCATGGTGGTGCTGA

Upstream 100 bases:

>100_bases
AGCTCGGGGTGAGCCCCAAGGTCCACGCCCTCTCCAGCCTGATTGTGGGGCTCAGCGCCTTCTTCCTCGCCCTGGGGTAT
GCTCTTAGCCGGAGGAGGTT

Downstream 100 bases:

>100_bases
AGCGGGGCCCGGCCCAGGAGCTGGCCTACCGCTTCATGGACTTCCTCCTGGAGCCGGAAAACGCCGCGGCCTTGGCGGAG
TACACCCACTACGCCACCCC

Product: spermidine/putrescine ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRRIAVLLLLVAVVLLGWLWLRPKAPSGAGTLYFLNWADYIPEELIQKFEAETGAKVVLDTFESPEAMLAKLKAGADQEF
SLVVAPDYYVLQMARDGLIAPLDKGKLKNLANLDPFFLDPPYDPGLQYSVPYLWGTTGIAYREDLVTGPVDSYAVFFAPE
AQVGPFLLLDEMRETIGAALKYLGYSVNTTDPEALAKAKELLLAAKERSVGFAGGLEALNRILAGDAALSLAYSGDVLQA
REEDQRLRYTLAKEGGRSGRTPWWC

Sequences:

>Translated_265_residues
MRRIAVLLLLVAVVLLGWLWLRPKAPSGAGTLYFLNWADYIPEELIQKFEAETGAKVVLDTFESPEAMLAKLKAGADQEF
SLVVAPDYYVLQMARDGLIAPLDKGKLKNLANLDPFFLDPPYDPGLQYSVPYLWGTTGIAYREDLVTGPVDSYAVFFAPE
AQVGPFLLLDEMRETIGAALKYLGYSVNTTDPEALAKAKELLLAAKERSVGFAGGLEALNRILAGDAALSLAYSGDVLQA
REEDQRLRYTLAKEGGRSGRTPWWC
>Mature_265_residues
MRRIAVLLLLVAVVLLGWLWLRPKAPSGAGTLYFLNWADYIPEELIQKFEAETGAKVVLDTFESPEAMLAKLKAGADQEF
SLVVAPDYYVLQMARDGLIAPLDKGKLKNLANLDPFFLDPPYDPGLQYSVPYLWGTTGIAYREDLVTGPVDSYAVFFAPE
AQVGPFLLLDEMRETIGAALKYLGYSVNTTDPEALAKAKELLLAAKERSVGFAGGLEALNRILAGDAALSLAYSGDVLQA
REEDQRLRYTLAKEGGRSGRTPWWC

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=228, Percent_Identity=40.7894736842105, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787078, Length=244, Percent_Identity=32.3770491803279, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 29125; Mature: 29125

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIAVLLLLVAVVLLGWLWLRPKAPSGAGTLYFLNWADYIPEELIQKFEAETGAKVVLD
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHCHHHHHHHHCCCCCCEEEEE
TFESPEAMLAKLKAGADQEFSLVVAPDYYVLQMARDGLIAPLDKGKLKNLANLDPFFLDP
CCCCHHHHHHHHHCCCCCCEEEEECCCHHEEHHHHCCCCCCCCCCHHHHHCCCCCCCCCC
PYDPGLQYSVPYLWGTTGIAYREDLVTGPVDSYAVFFAPEAQVGPFLLLDEMRETIGAAL
CCCCCCCEECCEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
KYLGYSVNTTDPEALAKAKELLLAAKERSVGFAGGLEALNRILAGDAALSLAYSGDVLQA
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHEEEEECCCHHHH
REEDQRLRYTLAKEGGRSGRTPWWC
HHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRRIAVLLLLVAVVLLGWLWLRPKAPSGAGTLYFLNWADYIPEELIQKFEAETGAKVVLD
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHCHHHHHHHHCCCCCCEEEEE
TFESPEAMLAKLKAGADQEFSLVVAPDYYVLQMARDGLIAPLDKGKLKNLANLDPFFLDP
CCCCHHHHHHHHHCCCCCCEEEEECCCHHEEHHHHCCCCCCCCCCHHHHHCCCCCCCCCC
PYDPGLQYSVPYLWGTTGIAYREDLVTGPVDSYAVFFAPEAQVGPFLLLDEMRETIGAAL
CCCCCCCEECCEEECCCCCHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
KYLGYSVNTTDPEALAKAKELLLAAKERSVGFAGGLEALNRILAGDAALSLAYSGDVLQA
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHEEEEECCCHHHH
REEDQRLRYTLAKEGGRSGRTPWWC
HHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]