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The map label for this gene is mdeA [H]

Identifier: 55981125

GI number: 55981125

Start: 1100627

End: 1101865

Strand: Reverse

Name: mdeA [H]

Synonym: TTHA1156

Alternate gene names: 55981125

Gene position: 1101865-1100627 (Counterclockwise)

Preceding gene: 55981130

Following gene: 55981124

Centisome position: 59.57

GC content: 70.86

Gene sequence:

>1239_bases
ATGGAGTACACCACCCTCGCGGTCCTCGCCGGGCTTCCCGAAGACCCCCACGGGGCGGTGGGGCTTCCCATCTACGCCGT
GGCCGCCTACGGCTTCAAAACCCTGGAAGAGGGCCAGGAGCGCTTCGCCACCGGGGAGGGCTACGTCTACGCGCGCCAGA
AGGACCCCACGGCCAAAGCCCTGGAGGAGAGGCTCAAGGCCCTGGAGGGGGCGCTTGAGGCCGTGGTCCTGGCCTCGGGC
CAGGCGGCCACCTTCGCCGCCCTCCTCGCCCTCCTCCGCCCGGGGGACGAGGTGGTGGCGGCCAAGGGGCTTTTCGGCCA
GACCATTGGCCTTTTCGGCCAGGTGCTCTCCCTCATGGGGGTAACGGTGCGCTACGTGGACCCCGAGCCCGAGGCCGTGC
GGGAGGCCCTGAGCGCAAAGACCCGCGCGGTCTTCGTGGAGACCGTGGCGAACCCCGCCCTTCTCGTACCCGACCTCGAG
GCCCTGGCCACCCTGGCCGAGGAAGCGGGCGTGGCCCTGGTCGTGGACAACACCTTCGGGGCGGCGGGGGCGCTCTGCCG
GCCTTTGGCCTGGGGGGCCCACGTGGTGGTGGAGAGCCTCACCAAGTGGGCCTCGGGGCACGGCTCCGTTTTGGGCGGGG
CGGTGCTTTCCCGGGAAACCGAGCTTTGGCGGAACTACCCCCAGTTTCTGCAGCCCGACCTCAAGGGCCAGATCCCCTGG
GAGGCCCTTAGGGCCAGGTGCTTCCCCGAAAGGGTCCGCACCTTGGGGCTTTCCCTCTGCGGCATGGCCCTTTCCCCCTT
CAACGCCTACCTCCTCTTCCAGGGCCTGGAGACCGTGGCCCTGAGGGTTGCGCGGATGAGCGAGACCGCGCGCTTCCTCG
CCGAGCGCCTCCAGGGCCACCCCAAGGTGAAGGCCCTCCGCTACCCGGGCCTCCCCGAGGACCCCGCCCACAGGAACGCC
CGGAAGTACCTGGCCTCGGGCGGGCCCATCCTCACCCTGGACCTGGGGGACCTGGAGAGGGCGAGCCGCTTCCTTGGGGC
CATCCGCCTCCTCAAGGCGGCGAACCTCGGGGACGCCCGGACCCTCTTGGTGCACCCCTGGACCACCACCCACAGTCGCC
TCAAGGAGGAGGCGAGGCTCCAGGCCGGGGTGACCCCAGGGCTCGTGCGGGTCTCCGTGGGCCTCGAGGACCCTCTGGAC
CTCCTCGCTCTCTTTGAGGAGGCCCTGGAGGCGGTCTAG

Upstream 100 bases:

>100_bases
CATACACACCTCCTTGGTGCCGCCGGTAGTATACCCGGCCCTTGCCCTTCTGGCAAGCGCCGCGCGGCCCGGGGAGGGGT
TTTTCGCTAGACTAGGGGGT

Downstream 100 bases:

>100_bases
ATGCCCTACACCATCGGCGAGCTCGCCCGGGCGTTTGGCCTTTCGCCTGATGCCCTCCGCTACTACGAAAGGCTTGGGCT
CCTCGCCCCCAGCGGCCGTT

Product: O-acetylhomoserine (thiol)-lyase/O-acetylserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MEYTTLAVLAGLPEDPHGAVGLPIYAVAAYGFKTLEEGQERFATGEGYVYARQKDPTAKALEERLKALEGALEAVVLASG
QAATFAALLALLRPGDEVVAAKGLFGQTIGLFGQVLSLMGVTVRYVDPEPEAVREALSAKTRAVFVETVANPALLVPDLE
ALATLAEEAGVALVVDNTFGAAGALCRPLAWGAHVVVESLTKWASGHGSVLGGAVLSRETELWRNYPQFLQPDLKGQIPW
EALRARCFPERVRTLGLSLCGMALSPFNAYLLFQGLETVALRVARMSETARFLAERLQGHPKVKALRYPGLPEDPAHRNA
RKYLASGGPILTLDLGDLERASRFLGAIRLLKAANLGDARTLLVHPWTTTHSRLKEEARLQAGVTPGLVRVSVGLEDPLD
LLALFEEALEAV

Sequences:

>Translated_412_residues
MEYTTLAVLAGLPEDPHGAVGLPIYAVAAYGFKTLEEGQERFATGEGYVYARQKDPTAKALEERLKALEGALEAVVLASG
QAATFAALLALLRPGDEVVAAKGLFGQTIGLFGQVLSLMGVTVRYVDPEPEAVREALSAKTRAVFVETVANPALLVPDLE
ALATLAEEAGVALVVDNTFGAAGALCRPLAWGAHVVVESLTKWASGHGSVLGGAVLSRETELWRNYPQFLQPDLKGQIPW
EALRARCFPERVRTLGLSLCGMALSPFNAYLLFQGLETVALRVARMSETARFLAERLQGHPKVKALRYPGLPEDPAHRNA
RKYLASGGPILTLDLGDLERASRFLGAIRLLKAANLGDARTLLVHPWTTTHSRLKEEARLQAGVTPGLVRVSVGLEDPLD
LLALFEEALEAV
>Mature_412_residues
MEYTTLAVLAGLPEDPHGAVGLPIYAVAAYGFKTLEEGQERFATGEGYVYARQKDPTAKALEERLKALEGALEAVVLASG
QAATFAALLALLRPGDEVVAAKGLFGQTIGLFGQVLSLMGVTVRYVDPEPEAVREALSAKTRAVFVETVANPALLVPDLE
ALATLAEEAGVALVVDNTFGAAGALCRPLAWGAHVVVESLTKWASGHGSVLGGAVLSRETELWRNYPQFLQPDLKGQIPW
EALRARCFPERVRTLGLSLCGMALSPFNAYLLFQGLETVALRVARMSETARFLAERLQGHPKVKALRYPGLPEDPAHRNA
RKYLASGGPILTLDLGDLERASRFLGAIRLLKAANLGDARTLLVHPWTTTHSRLKEEARLQAGVTPGLVRVSVGLEDPLD
LLALFEEALEAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=370, Percent_Identity=32.972972972973, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI299473759, Length=369, Percent_Identity=30.0813008130081, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI25453487, Length=368, Percent_Identity=29.0760869565217, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1790375, Length=411, Percent_Identity=33.0900243309002, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=24.6851385390428, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=404, Percent_Identity=30.6930693069307, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17538055, Length=364, Percent_Identity=30.4945054945055, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560056, Length=355, Percent_Identity=30.7042253521127, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=420, Percent_Identity=38.0952380952381, Blast_Score=265, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=32.8605200945626, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321254, Length=424, Percent_Identity=25.4716981132075, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=334, Percent_Identity=25.748502994012, Blast_Score=93, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321904, Length=372, Percent_Identity=25, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=31.7708333333333, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44185; Mature: 44185

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYTTLAVLAGLPEDPHGAVGLPIYAVAAYGFKTLEEGQERFATGEGYVYARQKDPTAKA
CCCHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LEERLKALEGALEAVVLASGQAATFAALLALLRPGDEVVAAKGLFGQTIGLFGQVLSLMG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHC
VTVRYVDPEPEAVREALSAKTRAVFVETVANPALLVPDLEALATLAEEAGVALVVDNTFG
CEEEEECCCHHHHHHHHHHHHHHEEEEHHCCCEEECCCHHHHHHHHHHCCEEEEEECCCC
AAGALCRPLAWGAHVVVESLTKWASGHGSVLGGAVLSRETELWRNYPQFLQPDLKGQIPW
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCH
EALRARCFPERVRTLGLSLCGMALSPFNAYLLFQGLETVALRVARMSETARFLAERLQGH
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKVKALRYPGLPEDPAHRNARKYLASGGPILTLDLGDLERASRFLGAIRLLKAANLGDAR
CCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCE
TLLVHPWTTTHSRLKEEARLQAGVTPGLVRVSVGLEDPLDLLALFEEALEAV
EEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEYTTLAVLAGLPEDPHGAVGLPIYAVAAYGFKTLEEGQERFATGEGYVYARQKDPTAKA
CCCHHEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHH
LEERLKALEGALEAVVLASGQAATFAALLALLRPGDEVVAAKGLFGQTIGLFGQVLSLMG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHC
VTVRYVDPEPEAVREALSAKTRAVFVETVANPALLVPDLEALATLAEEAGVALVVDNTFG
CEEEEECCCHHHHHHHHHHHHHHEEEEHHCCCEEECCCHHHHHHHHHHCCEEEEEECCCC
AAGALCRPLAWGAHVVVESLTKWASGHGSVLGGAVLSRETELWRNYPQFLQPDLKGQIPW
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCH
EALRARCFPERVRTLGLSLCGMALSPFNAYLLFQGLETVALRVARMSETARFLAERLQGH
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKVKALRYPGLPEDPAHRNARKYLASGGPILTLDLGDLERASRFLGAIRLLKAANLGDAR
CCEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCE
TLLVHPWTTTHSRLKEEARLQAGVTPGLVRVSVGLEDPLDLLALFEEALEAV
EEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]