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The map label for this gene is kipI [H]

Identifier: 55980957

GI number: 55980957

Start: 932553

End: 934037

Strand: Reverse

Name: kipI [H]

Synonym: TTHA0988

Alternate gene names: 55980957

Gene position: 934037-932553 (Counterclockwise)

Preceding gene: 55980961

Following gene: 55980954

Centisome position: 50.5

GC content: 74.68

Gene sequence:

>1485_bases
GTGGTCCGGGGTTTTTACCTGCGCTTCGGCGAGGGGGTCTCTGAGGAGGCGAACCGCAGGGCCCTCGCCCTGGCCGAGGC
CCTCCTCCGTGCCCCCCCTCCGGGGCTTCTGGACGCCGTGCCCGCCTACGGCGTCCTCTACCTGGAGTACGACCCCAGGC
GGCTTTCCCGAGGGCGGCTTTTGCGCCTTCTTAAGGGGCTTCCCCAGGAGCGGGCCGAGGAGGGGCGGGTGGTGGAGATC
CCCGTGCGCTACGACGGGGAGGACCTTCCCGAGGTGGCCTCCCGCCTGGGCCTCTCCCTCGAGGCGGTGAAGGCCCTCCA
CCAAAAGCCCCTCTACCGCGTCTACGCCCTGGGGTTCACCCCAGGCTTCCCCTTCCTGGCGGAGGTGGAGCCCGCCTTGC
GCCTTCCCCGGAAGCCCCACCCGAGGCCCCGGGTGCCCGCCCACGCCGTGGCCGTGGCGGGGGTGCAGACGGGGATCTAC
CCCCTTCCTTCTCCCGGGGGCTGGAACCTGCTCGGGACCAGCCTGGTGGCCGTCTACGACCCCCACCGCGAGACGCCCTT
CCTCCTCCGCCCCGGGGACCGGGTGCGCTTCCTGGAGGCCGAGGGGCCCACGCCGCCCGAGCCCCGCCCCCTGGAGCTCC
TCCCCGAGGAGCCCAGGCTTCCCGCCCTCCTCGTGGAGGAGCCCGGGCTTATGGACCTGGTGGTGGACGGGGGCCGCTTC
CTCGGGGGGCACCTCGGCCTCGCCCGCTCCGGTCCCCTGGACGCCCCTTCCGCCCGGTTGGCCAACCGGCTCGTGGGCAA
CGGGGCGGGGGCGCCCCTCTTGGAGTTCGCCTACAAAGGCCCCGTCCTCACCGCCCTCCGCGACCTGGTGGCGGCCTTCG
CGGGCTACGGCTTCGTCGCCCTTCTGGAAGGGGAGGAGATCCCCCCGGGGCAAAGCTTCCTCTGGCCCCGGGGCAAGACC
CTCCGCTTCCGCCCCCGGGGCCCGGGGGTGCGGGGCTACCTGGCGGTGGCGGGGGGCCTCGAGGTCCGGCCCTTCCTCGG
CTCCGCCTCCCCGGACCTCCGGGGGAGGATCGGGAGGCCCTTGTGGGCAGGGGATGTCCTGGGCCTCGAGGCGCTTCGCC
CGGTCCGCCCCGGGAGGGCCTTCCCCCAGCGCCCGCTCCCCGAGGCCTTCCGCCTCCGCCTCCTCCCCGGGCCCCAGTTT
GCGGGGGAGGCCTTTAGGGCCCTTTGCTCCGGCCCCTTCCGGGTAGCGCGGGCGGACCGGGTGGGGGTGGAGCTTTTGGG
GCCGGAGGTCCCTGGCGGGGAGGGGCTTTCCGAGCCCACCCCCCTCGGCGGGGTCCAGGTGCCCCCCTCGGGGAGGCCCC
TGGTGCTCCTCGCCGACAAGGGGAGCCTCGGGGGGTACGCCAAGCCCGCCCTGGTGGACCCCCGGGACCTCTGGCTCCTG
GGGCAGGCCCGGCCTGGTGTGGAGATACACTTCACGAGTGGGTGA

Upstream 100 bases:

>100_bases
TCAAAAAGTGTAGCCCTCGTCATTCTTGGGGCCTTGGTCTTCGTCCTCCTCCTTCCCGACCGGGAGCTCAAGGGGAGGCC
CGCTCCGGTAGAGTGAGGGG

Downstream 100 bases:

>100_bases
AATCGTGAATAAAAGTACAAGATGGCCAAAGAACCCCCTTCAGCCCCCGGGGAGGGGCCTAAGACTCAGCCCATCCCCTC
CACCACCACGGCGATGCCCT

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MVRGFYLRFGEGVSEEANRRALALAEALLRAPPPGLLDAVPAYGVLYLEYDPRRLSRGRLLRLLKGLPQERAEEGRVVEI
PVRYDGEDLPEVASRLGLSLEAVKALHQKPLYRVYALGFTPGFPFLAEVEPALRLPRKPHPRPRVPAHAVAVAGVQTGIY
PLPSPGGWNLLGTSLVAVYDPHRETPFLLRPGDRVRFLEAEGPTPPEPRPLELLPEEPRLPALLVEEPGLMDLVVDGGRF
LGGHLGLARSGPLDAPSARLANRLVGNGAGAPLLEFAYKGPVLTALRDLVAAFAGYGFVALLEGEEIPPGQSFLWPRGKT
LRFRPRGPGVRGYLAVAGGLEVRPFLGSASPDLRGRIGRPLWAGDVLGLEALRPVRPGRAFPQRPLPEAFRLRLLPGPQF
AGEAFRALCSGPFRVARADRVGVELLGPEVPGGEGLSEPTPLGGVQVPPSGRPLVLLADKGSLGGYAKPALVDPRDLWLL
GQARPGVEIHFTSG

Sequences:

>Translated_494_residues
MVRGFYLRFGEGVSEEANRRALALAEALLRAPPPGLLDAVPAYGVLYLEYDPRRLSRGRLLRLLKGLPQERAEEGRVVEI
PVRYDGEDLPEVASRLGLSLEAVKALHQKPLYRVYALGFTPGFPFLAEVEPALRLPRKPHPRPRVPAHAVAVAGVQTGIY
PLPSPGGWNLLGTSLVAVYDPHRETPFLLRPGDRVRFLEAEGPTPPEPRPLELLPEEPRLPALLVEEPGLMDLVVDGGRF
LGGHLGLARSGPLDAPSARLANRLVGNGAGAPLLEFAYKGPVLTALRDLVAAFAGYGFVALLEGEEIPPGQSFLWPRGKT
LRFRPRGPGVRGYLAVAGGLEVRPFLGSASPDLRGRIGRPLWAGDVLGLEALRPVRPGRAFPQRPLPEAFRLRLLPGPQF
AGEAFRALCSGPFRVARADRVGVELLGPEVPGGEGLSEPTPLGGVQVPPSGRPLVLLADKGSLGGYAKPALVDPRDLWLL
GQARPGVEIHFTSG
>Mature_494_residues
MVRGFYLRFGEGVSEEANRRALALAEALLRAPPPGLLDAVPAYGVLYLEYDPRRLSRGRLLRLLKGLPQERAEEGRVVEI
PVRYDGEDLPEVASRLGLSLEAVKALHQKPLYRVYALGFTPGFPFLAEVEPALRLPRKPHPRPRVPAHAVAVAGVQTGIY
PLPSPGGWNLLGTSLVAVYDPHRETPFLLRPGDRVRFLEAEGPTPPEPRPLELLPEEPRLPALLVEEPGLMDLVVDGGRF
LGGHLGLARSGPLDAPSARLANRLVGNGAGAPLLEFAYKGPVLTALRDLVAAFAGYGFVALLEGEEIPPGQSFLWPRGKT
LRFRPRGPGVRGYLAVAGGLEVRPFLGSASPDLRGRIGRPLWAGDVLGLEALRPVRPGRAFPQRPLPEAFRLRLLPGPQF
AGEAFRALCSGPFRVARADRVGVELLGPEVPGGEGLSEPTPLGGVQVPPSGRPLVLLADKGSLGGYAKPALVDPRDLWLL
GQARPGVEIHFTSG

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=192, Percent_Identity=39.5833333333333, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786930, Length=283, Percent_Identity=33.9222614840989, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319685, Length=302, Percent_Identity=32.4503311258278, Blast_Score=116, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 53092; Mature: 53092

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRGFYLRFGEGVSEEANRRALALAEALLRAPPPGLLDAVPAYGVLYLEYDPRRLSRGRL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEECHHHHHHHHH
LRLLKGLPQERAEEGRVVEIPVRYDGEDLPEVASRLGLSLEAVKALHQKPLYRVYALGFT
HHHHHCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECC
PGFPFLAEVEPALRLPRKPHPRPRVPAHAVAVAGVQTGIYPLPSPGGWNLLGTSLVAVYD
CCCCHHHCCCHHHHCCCCCCCCCCCCHHHEEEHHHHCCCEECCCCCCCCHHCCEEEEEEC
PHRETPFLLRPGDRVRFLEAEGPTPPEPRPLELLPEEPRLPALLVEEPGLMDLVVDGGRF
CCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCEE
LGGHLGLARSGPLDAPSARLANRLVGNGAGAPLLEFAYKGPVLTALRDLVAAFAGYGFVA
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEE
LLEGEEIPPGQSFLWPRGKTLRFRPRGPGVRGYLAVAGGLEVRPFLGSASPDLRGRIGRP
EEECCCCCCCCCEECCCCCEEEECCCCCCCEEEEEEECCCEEEECCCCCCCCHHHCCCCC
LWAGDVLGLEALRPVRPGRAFPQRPLPEAFRLRLLPGPQFAGEAFRALCSGPFRVARADR
CCCCCHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHHCCCCEEEHHCC
VGVELLGPEVPGGEGLSEPTPLGGVQVPPSGRPLVLLADKGSLGGYAKPALVDPRDLWLL
CCEEEECCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEE
GQARPGVEIHFTSG
ECCCCCEEEEECCC
>Mature Secondary Structure
MVRGFYLRFGEGVSEEANRRALALAEALLRAPPPGLLDAVPAYGVLYLEYDPRRLSRGRL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEECHHHHHHHHH
LRLLKGLPQERAEEGRVVEIPVRYDGEDLPEVASRLGLSLEAVKALHQKPLYRVYALGFT
HHHHHCCCHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECC
PGFPFLAEVEPALRLPRKPHPRPRVPAHAVAVAGVQTGIYPLPSPGGWNLLGTSLVAVYD
CCCCHHHCCCHHHHCCCCCCCCCCCCHHHEEEHHHHCCCEECCCCCCCCHHCCEEEEEEC
PHRETPFLLRPGDRVRFLEAEGPTPPEPRPLELLPEEPRLPALLVEEPGLMDLVVDGGRF
CCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCEE
LGGHLGLARSGPLDAPSARLANRLVGNGAGAPLLEFAYKGPVLTALRDLVAAFAGYGFVA
ECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEE
LLEGEEIPPGQSFLWPRGKTLRFRPRGPGVRGYLAVAGGLEVRPFLGSASPDLRGRIGRP
EEECCCCCCCCCEECCCCCEEEECCCCCCCEEEEEEECCCEEEECCCCCCCCHHHCCCCC
LWAGDVLGLEALRPVRPGRAFPQRPLPEAFRLRLLPGPQFAGEAFRALCSGPFRVARADR
CCCCCHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHHCCCCEEEHHCC
VGVELLGPEVPGGEGLSEPTPLGGVQVPPSGRPLVLLADKGSLGGYAKPALVDPRDLWLL
CCEEEECCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCEECCCCEEEE
GQARPGVEIHFTSG
ECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]