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The map label for this gene is dosC [H]

Identifier: 55980767

GI number: 55980767

Start: 762972

End: 763922

Strand: Direct

Name: dosC [H]

Synonym: TTHA0798

Alternate gene names: 55980767

Gene position: 762972-763922 (Clockwise)

Preceding gene: 55980766

Following gene: 55980777

Centisome position: 41.25

GC content: 71.71

Gene sequence:

>951_bases
ATGGAAGGCCTCTACCGCCTCTTTTATGTCCTCGCCTGGATGGGCCTTCCTTTGGGCATTGGGCTTCAGCTCTTCTGGCA
TCCGCCTAGGGGCGGGGAGTGGGGGTTCTACCTTCTCTTCGCCTTCCTTTTTGGGCTCGCCCTTCTCCAGGGGCCGAGGC
GGGCCCCCCGCTTCCTCGCCCACGCCCTGGGGGCTTACCTCCTCTTTGAGCTTTGGCGGGGGAGGGACTTCCCCTGGGCG
GTGGGCTTCTGGTCCCCTTCCCTCTACCTCCTCCTGGCCTTCGCCTACCCTCTCCCCCTGGCCTTGGCCTTGGGCCTCCT
TTGGGCGGCGCTCCTCTCCGCCTTCGCCCTTTGGGCCGCGGGGGGGCCCACCTGGCCTTGGGGGCACTTCGCTTCAAGCC
AGCCCGTCCTCCTCGCCCTCACCCTGCTCCTCGCCCGCTTCCGGGAGCTCTACGGCCAGGTGCGCTTCTGGCGGGAGCGG
GCCCTCACTTGTCCCCTCACGGGCCTCCCCAACCGCAGGGCCTTGGAGATGGCCCTAGAGCGGGAGGCGGCCCGGGTGGA
GCGGGGCGGGCCCCCCTTCGCCCTGGTCCTTTTGGACCTGGACGGCTTCAAGCGGGTGAACGACGAGGGGGGGCACAGGG
AGGGGGACCGGTACCTCAGGCGGGTGGCCCGGGCGTTGGTCTCGGGGGTGCGCCGGGGGGACCTCGTGGCCCGGTGGGGC
GGGGACGAGTTCGCCGTCCTCCTCCCGGGGACGGGGGAGAAGGAGGCGGTGGAGGTGGCGGAGCGGCTTGGGCGCCGCCT
CCAGGAGGAGGGGGTCTCGGCGAGCCTGGGGGTGGCCGTGTACGCGGGGGACCTCGAGGACCTCTTCCTCCGGGCCGACC
AGGCCCTCTACCGGGCCAAGGCTCTGGGGAAGGGCCGGGTGGTCCTCGCCCCGCCTCAGGGCAGGCGGTAG

Upstream 100 bases:

>100_bases
GCGCTGGACCCCCTGGGCCTCTTCAACCCAGGGAAGGTCCTTCCCTAAGCCCTGTTCGTTTTTCACGGCGTTTTGACGGC
GGGGAGGCCATAATGGGGCC

Downstream 100 bases:

>100_bases
CCCGCCTCCTGGAGGACGCGCCGGGCCCTTTCCCGCTCCTCCCGGGTGGCGAAGGAGAGGCGGAGGGCCCCCGCCGCCTC
CCGGATGGTGAGGACCTCTA

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MEGLYRLFYVLAWMGLPLGIGLQLFWHPPRGGEWGFYLLFAFLFGLALLQGPRRAPRFLAHALGAYLLFELWRGRDFPWA
VGFWSPSLYLLLAFAYPLPLALALGLLWAALLSAFALWAAGGPTWPWGHFASSQPVLLALTLLLARFRELYGQVRFWRER
ALTCPLTGLPNRRALEMALEREAARVERGGPPFALVLLDLDGFKRVNDEGGHREGDRYLRRVARALVSGVRRGDLVARWG
GDEFAVLLPGTGEKEAVEVAERLGRRLQEEGVSASLGVAVYAGDLEDLFLRADQALYRAKALGKGRVVLAPPQGRR

Sequences:

>Translated_316_residues
MEGLYRLFYVLAWMGLPLGIGLQLFWHPPRGGEWGFYLLFAFLFGLALLQGPRRAPRFLAHALGAYLLFELWRGRDFPWA
VGFWSPSLYLLLAFAYPLPLALALGLLWAALLSAFALWAAGGPTWPWGHFASSQPVLLALTLLLARFRELYGQVRFWRER
ALTCPLTGLPNRRALEMALEREAARVERGGPPFALVLLDLDGFKRVNDEGGHREGDRYLRRVARALVSGVRRGDLVARWG
GDEFAVLLPGTGEKEAVEVAERLGRRLQEEGVSASLGVAVYAGDLEDLFLRADQALYRAKALGKGRVVLAPPQGRR
>Mature_316_residues
MEGLYRLFYVLAWMGLPLGIGLQLFWHPPRGGEWGFYLLFAFLFGLALLQGPRRAPRFLAHALGAYLLFELWRGRDFPWA
VGFWSPSLYLLLAFAYPLPLALALGLLWAALLSAFALWAAGGPTWPWGHFASSQPVLLALTLLLARFRELYGQVRFWRER
ALTCPLTGLPNRRALEMALEREAARVERGGPPFALVLLDLDGFKRVNDEGGHREGDRYLRRVARALVSGVRRGDLVARWG
GDEFAVLLPGTGEKEAVEVAERLGRRLQEEGVSASLGVAVYAGDLEDLFLRADQALYRAKALGKGRVVLAPPQGRR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=183, Percent_Identity=37.1584699453552, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=153, Percent_Identity=40.5228758169935, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787262, Length=201, Percent_Identity=28.8557213930348, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=157, Percent_Identity=40.1273885350318, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081974, Length=176, Percent_Identity=31.25, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=35.1190476190476, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=36.875, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=203, Percent_Identity=30.5418719211823, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786584, Length=156, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=26.2569832402235, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787802, Length=157, Percent_Identity=30.5732484076433, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=216, Percent_Identity=28.2407407407407, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35251; Mature: 35251

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50887 GGDEF ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLYRLFYVLAWMGLPLGIGLQLFWHPPRGGEWGFYLLFAFLFGLALLQGPRRAPRFLA
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
HALGAYLLFELWRGRDFPWAVGFWSPSLYLLLAFAYPLPLALALGLLWAALLSAFALWAA
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGPTWPWGHFASSQPVLLALTLLLARFRELYGQVRFWRERALTCPLTGLPNRRALEMALE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
REAARVERGGPPFALVLLDLDGFKRVNDEGGHREGDRYLRRVARALVSGVRRGDLVARWG
HHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
GDEFAVLLPGTGEKEAVEVAERLGRRLQEEGVSASLGVAVYAGDLEDLFLRADQALYRAK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
ALGKGRVVLAPPQGRR
HCCCCCEEECCCCCCC
>Mature Secondary Structure
MEGLYRLFYVLAWMGLPLGIGLQLFWHPPRGGEWGFYLLFAFLFGLALLQGPRRAPRFLA
CCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
HALGAYLLFELWRGRDFPWAVGFWSPSLYLLLAFAYPLPLALALGLLWAALLSAFALWAA
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGPTWPWGHFASSQPVLLALTLLLARFRELYGQVRFWRERALTCPLTGLPNRRALEMALE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
REAARVERGGPPFALVLLDLDGFKRVNDEGGHREGDRYLRRVARALVSGVRRGDLVARWG
HHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
GDEFAVLLPGTGEKEAVEVAERLGRRLQEEGVSASLGVAVYAGDLEDLFLRADQALYRAK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
ALGKGRVVLAPPQGRR
HCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]