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The map label for this gene is thiE

Identifier: 55980643

GI number: 55980643

Start: 640222

End: 640842

Strand: Direct

Name: thiE

Synonym: TTHA0674

Alternate gene names: 55980643

Gene position: 640222-640842 (Clockwise)

Preceding gene: 55980634

Following gene: 55980644

Centisome position: 34.61

GC content: 71.66

Gene sequence:

>621_bases
TTGCTTGGAAGGCTCTACCTGGTGGTGACCCCAAGGCCCGGCTGGTCTTTGGAGGCTACCCTGGACCGCACGGAAAGGGC
CTTGGCGGGCGGCGTGGAGGTCGTGCAGCTGAGGGCCAAGGACTGGGAGGCGCGGCCCACCCTGGCCCTCGGGGAAAGGA
TGCTCGCCCTCGCCCGGCGCTACGGGGTGCCCTTCTTCCTGAACGACCGCCCCGACCTCGCCGCCCTCCTGGGGGCGGAC
GGGGTGCACCTGGGGCAGAACGACCTCACCCCCGAGGAGGCGCGGCGCTTCTTTGCCGGGATGGTGGGCCGCTCCACCCA
CGCCCCGGAGCAGGCGCTAAGGGCCCTAGAGGAGGGGGTGGACTACCTCTCCGTGGGCCCCGTCTGGGAGACCCCCACGA
AGCCGGGCAGGCCCGCGGCGGGGCTCGCTTACGTGCGCTGGGCGGCGGAGAACCTGGGGGAGAAGCCCTGGTACGCCATC
GGGGGGATTGACCTGGGGAACCTGGACCAGGTCCTAGAGGCCGGGGCCAGGCGCATCGTGGTGGTCCGGGCCATCCTGGA
CGCCCCTGACCCCGAGCGGGCGGCCCGCGCCCTTAGGGAGAGGCTTTATGGTGTGGCTTAA

Upstream 100 bases:

>100_bases
GCCGGCGGAGGGAAGCCTATGCGGAAGACCCGAATCCCCCAAAACCTAGCCCCCTTCGGGCCGCGTGGGCCCTTCCCGCT
GGCCTAGAGGGGGTGGAAGG

Downstream 100 bases:

>100_bases
CGGGGAGCCCAGGCCCTTGGAGGGAAAGACCCTCAAGGAGGTCTTGGAGGAAATGGGCGTGGAGCTTAAGGGGGTCGCCG
TCCTCCTCAACGAGGAGGCC

Product: thiamin-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MLGRLYLVVTPRPGWSLEATLDRTERALAGGVEVVQLRAKDWEARPTLALGERMLALARRYGVPFFLNDRPDLAALLGAD
GVHLGQNDLTPEEARRFFAGMVGRSTHAPEQALRALEEGVDYLSVGPVWETPTKPGRPAAGLAYVRWAAENLGEKPWYAI
GGIDLGNLDQVLEAGARRIVVVRAILDAPDPERAARALRERLYGVA

Sequences:

>Translated_206_residues
MLGRLYLVVTPRPGWSLEATLDRTERALAGGVEVVQLRAKDWEARPTLALGERMLALARRYGVPFFLNDRPDLAALLGAD
GVHLGQNDLTPEEARRFFAGMVGRSTHAPEQALRALEEGVDYLSVGPVWETPTKPGRPAAGLAYVRWAAENLGEKPWYAI
GGIDLGNLDQVLEAGARRIVVVRAILDAPDPERAARALRERLYGVA
>Mature_206_residues
MLGRLYLVVTPRPGWSLEATLDRTERALAGGVEVVQLRAKDWEARPTLALGERMLALARRYGVPFFLNDRPDLAALLGAD
GVHLGQNDLTPEEARRFFAGMVGRSTHAPEQALRALEEGVDYLSVGPVWETPTKPGRPAAGLAYVRWAAENLGEKPWYAI
GGIDLGNLDQVLEAGARRIVVVRAILDAPDPERAARALRERLYGVA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=170, Percent_Identity=39.4117647058824, Blast_Score=77, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=220, Percent_Identity=31.3636363636364, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_THET2 (Q72KL8)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_004290.1
- HSSP:   Q8U192
- ProteinModelPortal:   Q72KL8
- SMR:   Q72KL8
- STRING:   Q72KL8
- GeneID:   2775542
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- PhylomeDB:   Q72KL8
- ProtClustDB:   CLSK2761890
- BioCyc:   TTHE262724:TT_C0315-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22529; Mature: 22529

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRLYLVVTPRPGWSLEATLDRTERALAGGVEVVQLRAKDWEARPTLALGERMLALARR
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
YGVPFFLNDRPDLAALLGADGVHLGQNDLTPEEARRFFAGMVGRSTHAPEQALRALEEGV
HCCCEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
DYLSVGPVWETPTKPGRPAAGLAYVRWAAENLGEKPWYAIGGIDLGNLDQVLEAGARRIV
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCHHEE
VVRAILDAPDPERAARALRERLYGVA
EHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGRLYLVVTPRPGWSLEATLDRTERALAGGVEVVQLRAKDWEARPTLALGERMLALARR
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
YGVPFFLNDRPDLAALLGADGVHLGQNDLTPEEARRFFAGMVGRSTHAPEQALRALEEGV
HCCCEEECCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
DYLSVGPVWETPTKPGRPAAGLAYVRWAAENLGEKPWYAIGGIDLGNLDQVLEAGARRIV
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCHHEE
VVRAILDAPDPERAARALRERLYGVA
EHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA