Click here to switch to the map view.

The map label for this gene is punA [H]

Identifier: 55980531

GI number: 55980531

Start: 524534

End: 525361

Strand: Direct

Name: punA [H]

Synonym: TTHA0562

Alternate gene names: 55980531

Gene position: 524534-525361 (Clockwise)

Preceding gene: 55980528

Following gene: 55980532

Centisome position: 28.36

GC content: 69.57

Gene sequence:

>828_bases
ATGCCGGGTATGGAGCTCTACGACAAGATCCAGGAGGCCGTGGCCTACGTCCGCTCCAAGACGGACTTCGTCCCCGAGGT
GGGCCTGGTTTTGGGCTCGGGGCTCGGCCCCTTGGCCGACGAGGTGGAGAAGGTGGCGGAGATCCCCTACGGGGAGATCC
CCCACTTCCCCGTCTCCACCGCCCCGGGGCACGCCGGGAGGCTCGTCCTGGGGCGCCTCGAGGGCAAGCCCGTCTTGGTC
TACAAGGGCCGGGTCCACTACTACGAGGGCTACTCCGCCGAGGAGGTGGTCTTCCCCGTGCGGGTGGGCTTCTTCCTCGG
GGCCAGGACCTTCCTCCTCACCTCCGCCGCCGGGGGGCTCAACCCCCGCTTCCGGGCGGGCGGGATCATGCTCCACCTGG
ACTACATCAACTTCGCCGGGGCGAACCCCCTAAGGGGCCCGAACGACGAGCGCCTGGGCCCCCGCTTCCCCGTGATGTTT
GAGGCCTACGACCCGGAGCTCATAGAGCTCGCCCGCAAGGTGGCGCGCAGGCAGGACCTCCACCTCTTTGAGGGGGTCTA
CGCCTGGTTCATGGGGCCGAGCTTCGCCAGCCGGGCGGAGCTTAAGCTTCTGCGGGAGCTCGGGGCGGACGCCATCGGCA
TGTCCACCGTGCCCGAGGTCATCGCCCTCCGCCATCTGGGCGCCCGGGTTCTTGGGCTATCCACCATCACCGACATGGCC
GTGCCCGAACGGGAGCACCACGCCACGGAGGAGGAGGTCCTGCGCGTGGCCGCGGAGACGGGCCCCGTCTTCCGCCGGTA
TGTCCGGGGGATCCTCGCCGAGCTCTGA

Upstream 100 bases:

>100_bases
AGGGGGCCGCGAGGGCCGTAAGGGCCAGAAGGCCCAGGGCGAGAAGCCGCTTCATGGCCGGAAGCTTACCCAAAGGCCGT
TAAGCCTGTGTGAGAGTAAA

Downstream 100 bases:

>100_bases
GGCCGCGCGGCGCGGGAGGCGAGGGGCGCATGGGGCTTCCCCAGGTTCTGGAGCGCATCGCGCGGGCCTGCCGCTGCGCG
GGGCGCAGGCCCGAGGAGGT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPGMELYDKIQEAVAYVRSKTDFVPEVGLVLGSGLGPLADEVEKVAEIPYGEIPHFPVSTAPGHAGRLVLGRLEGKPVLV
YKGRVHYYEGYSAEEVVFPVRVGFFLGARTFLLTSAAGGLNPRFRAGGIMLHLDYINFAGANPLRGPNDERLGPRFPVMF
EAYDPELIELARKVARRQDLHLFEGVYAWFMGPSFASRAELKLLRELGADAIGMSTVPEVIALRHLGARVLGLSTITDMA
VPEREHHATEEEVLRVAAETGPVFRRYVRGILAEL

Sequences:

>Translated_275_residues
MPGMELYDKIQEAVAYVRSKTDFVPEVGLVLGSGLGPLADEVEKVAEIPYGEIPHFPVSTAPGHAGRLVLGRLEGKPVLV
YKGRVHYYEGYSAEEVVFPVRVGFFLGARTFLLTSAAGGLNPRFRAGGIMLHLDYINFAGANPLRGPNDERLGPRFPVMF
EAYDPELIELARKVARRQDLHLFEGVYAWFMGPSFASRAELKLLRELGADAIGMSTVPEVIALRHLGARVLGLSTITDMA
VPEREHHATEEEVLRVAAETGPVFRRYVRGILAEL
>Mature_274_residues
PGMELYDKIQEAVAYVRSKTDFVPEVGLVLGSGLGPLADEVEKVAEIPYGEIPHFPVSTAPGHAGRLVLGRLEGKPVLVY
KGRVHYYEGYSAEEVVFPVRVGFFLGARTFLLTSAAGGLNPRFRAGGIMLHLDYINFAGANPLRGPNDERLGPRFPVMFE
AYDPELIELARKVARRQDLHLFEGVYAWFMGPSFASRAELKLLRELGADAIGMSTVPEVIALRHLGARVLGLSTITDMAV
PEREHHATEEEVLRVAAETGPVFRRYVRGILAEL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=276, Percent_Identity=46.0144927536232, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1788746, Length=256, Percent_Identity=40.625, Blast_Score=196, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17541190, Length=278, Percent_Identity=39.568345323741, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323238, Length=298, Percent_Identity=39.261744966443, Blast_Score=193, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24656093, Length=280, Percent_Identity=41.7857142857143, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI45552885, Length=280, Percent_Identity=41.7857142857143, Blast_Score=223, Evalue=7e-59,
Organism=Drosophila melanogaster, GI45552887, Length=280, Percent_Identity=41.7857142857143, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24656090, Length=280, Percent_Identity=41.7857142857143, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24762376, Length=251, Percent_Identity=35.0597609561753, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 30283; Mature: 30152

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGMELYDKIQEAVAYVRSKTDFVPEVGLVLGSGLGPLADEVEKVAEIPYGEIPHFPVST
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
APGHAGRLVLGRLEGKPVLVYKGRVHYYEGYSAEEVVFPVRVGFFLGARTFLLTSAAGGL
CCCCCCCEEEEEECCCEEEEEECEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
NPRFRAGGIMLHLDYINFAGANPLRGPNDERLGPRFPVMFEAYDPELIELARKVARRQDL
CCCEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
HLFEGVYAWFMGPSFASRAELKLLRELGADAIGMSTVPEVIALRHLGARVLGLSTITDMA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPEREHHATEEEVLRVAAETGPVFRRYVRGILAEL
CCCHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGMELYDKIQEAVAYVRSKTDFVPEVGLVLGSGLGPLADEVEKVAEIPYGEIPHFPVST
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
APGHAGRLVLGRLEGKPVLVYKGRVHYYEGYSAEEVVFPVRVGFFLGARTFLLTSAAGGL
CCCCCCCEEEEEECCCEEEEEECEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
NPRFRAGGIMLHLDYINFAGANPLRGPNDERLGPRFPVMFEAYDPELIELARKVARRQDL
CCCEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
HLFEGVYAWFMGPSFASRAELKLLRELGADAIGMSTVPEVIALRHLGARVLGLSTITDMA
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPEREHHATEEEVLRVAAETGPVFRRYVRGILAEL
CCCHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]